##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674151
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	51850628
Total Bases	5.1 Gbp
Sequences flagged as poor quality	0
Sequence length	63-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.29848359792286	37.0	36.0	37.0	31.0	37.0
2	34.89176688467496	37.0	35.0	37.0	29.0	37.0
3	35.11321286214701	37.0	35.0	37.0	30.0	37.0
4	35.114537513412564	37.0	35.0	37.0	30.0	37.0
5	35.10816079218944	37.0	35.0	37.0	30.0	37.0
6	34.935412951989704	37.0	35.0	37.0	30.0	37.0
7	35.08063512750511	37.0	35.0	37.0	30.0	37.0
8	35.07893599668648	37.0	35.0	37.0	30.0	37.0
9	35.006557702637664	37.0	35.0	37.0	30.0	37.0
10-11	35.05415087740114	37.0	35.0	37.0	30.0	37.0
12-13	35.03829931433039	37.0	35.0	37.0	30.0	37.0
14-15	35.083376473280126	37.0	35.0	37.0	30.0	37.0
16-17	35.02899915696296	37.0	35.0	37.0	30.0	37.0
18-19	35.04215801397815	37.0	35.0	37.0	30.0	37.0
20-21	34.957121907954516	37.0	35.0	37.0	30.0	37.0
22-23	34.95238229322892	37.0	35.0	37.0	30.0	37.0
24-25	35.02112340857279	37.0	35.0	37.0	30.0	37.0
26-27	35.00885517722177	37.0	35.0	37.0	30.0	37.0
28-29	35.03239141095842	37.0	35.0	37.0	30.0	37.0
30-31	35.04235425075276	37.0	35.0	37.0	30.0	37.0
32-33	35.018027544430126	37.0	35.0	37.0	30.0	37.0
34-35	34.88618323967069	37.0	35.0	37.0	29.5	37.0
36-37	35.001093988678406	37.0	35.0	37.0	30.0	37.0
38-39	34.91328241000282	37.0	35.0	37.0	30.0	37.0
40-41	34.968989748783756	37.0	35.0	37.0	30.0	37.0
42-43	35.00853388892416	37.0	35.0	37.0	30.0	37.0
44-45	34.9912355642057	37.0	35.0	37.0	30.0	37.0
46-47	34.92306414302253	37.0	35.0	37.0	30.0	37.0
48-49	34.93974815502717	37.0	35.0	37.0	30.0	37.0
50-51	34.964432455861484	37.0	35.0	37.0	30.0	37.0
52-53	34.91346407607638	37.0	35.0	37.0	29.5	37.0
54-55	34.879552943891056	37.0	35.0	37.0	29.5	37.0
56-57	34.92233719907887	37.0	35.0	37.0	30.0	37.0
58-59	34.79135561289634	37.0	35.0	37.0	29.0	37.0
60-61	34.87110689382585	37.0	35.0	37.0	29.0	37.0
62-63	34.88670117939555	37.0	35.0	37.0	29.0	37.0
64-65	34.83953653850079	37.0	35.0	37.0	29.0	37.0
66-67	34.87727774009903	37.0	35.0	37.0	29.0	37.0
68-69	34.824515366718664	37.0	35.0	37.0	29.0	37.0
70-71	34.83330699163813	37.0	35.0	37.0	29.0	37.0
72-73	34.84329910956458	37.0	35.0	37.0	29.0	37.0
74-75	34.8207865449821	37.0	35.0	37.0	29.0	37.0
76-77	34.80759616947579	37.0	35.0	37.0	29.0	37.0
78-79	34.7072138263829	37.0	35.0	37.0	29.0	37.0
80-81	34.75971560866878	37.0	35.0	37.0	29.0	37.0
82-83	34.675441131909125	37.0	35.0	37.0	29.0	37.0
84-85	34.63447807030959	37.0	35.0	37.0	29.0	37.0
86-87	34.577383069728036	37.0	35.0	37.0	28.5	37.0
88-89	34.5861682400227	37.0	35.0	37.0	28.0	37.0
90-91	34.544550897847586	37.0	35.0	37.0	28.0	37.0
92-93	34.50035944179628	37.0	35.0	37.0	28.0	37.0
94-95	34.38049233567722	37.0	35.0	37.0	28.0	37.0
96-97	34.43750266813394	37.0	35.0	37.0	28.0	37.0
98-99	34.49930573064209	37.0	35.0	37.0	28.0	37.0
100	35.072653340020615	37.0	35.0	37.0	30.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	216.0
20	10640.0
21	55783.0
22	107331.0
23	163955.0
24	227877.0
25	301441.0
26	397970.0
27	531828.0
28	702575.0
29	923205.0
30	1213484.0
31	1606454.0
32	2187222.0
33	3158371.0
34	5112588.0
35	1.084945E7
36	2.3103228E7
37	1197010.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	24.813019794898757	29.075216895417228	21.80256353591672	24.309199773767293
2	26.050200106766553	31.283279084411326	26.06925908798744	16.597261720834684
3	16.69034608543981	27.886572000036026	31.07545075353666	24.347631160987497
4	19.139609789376237	29.390048986094143	29.74590851755641	21.724432706973214
5	21.195887994672677	27.66412525880636	29.42994179490635	21.710044951614613
6	20.935831013459172	27.455688936362282	29.586272716596667	22.02220733358188
7	21.709581788951105	28.702102350779235	28.699675402407525	20.888640457862138
8	19.824909095478287	27.066635825935226	30.46175599186417	22.646699086722315
9	20.91262015237723	26.575900712086764	30.217625831653134	22.293853303882873
10-11	21.96392785803222	27.515369665347865	28.41222918915146	22.10847328746845
12-13	21.997230359382815	27.761443271918317	27.99903304153874	22.242293327160123
14-15	22.007249656327147	27.91382294472949	27.91864742152689	22.160279977416472
16-17	22.17511638681036	27.929001591046855	27.85495650320074	22.040925518942053
18-19	22.113312635028336	27.790250626344765	27.840082760557305	22.256353978069598
20-21	22.11871735931215	27.743901369981682	27.930098264245267	22.207283006460898
22-23	22.240499262869555	27.958666961056245	27.745290248363276	22.055543527710928
24-25	22.161396103736994	27.839363107990163	27.793652195055714	22.205588593217136
26-27	22.15166806459152	27.757549300654595	27.914650161757393	22.176132472996493
28-29	22.300475727629102	27.83534766425122	27.73981732043611	22.124359287683568
30-31	22.22265916377378	27.73369741472045	27.7547320038762	22.288911417629574
32-33	22.190247510601825	27.743140793341052	27.8255494417116	22.241062254345525
34-35	22.31198937870082	27.851262551831553	27.679317898757144	22.157430170710487
36-37	22.259239648913358	27.73364367255113	27.72105815482649	22.286058523709027
38-39	22.228327090704585	27.702948932977524	27.807182076568214	22.261541899749677
40-41	22.34919342313148	27.78598889632336	27.671587894171818	22.193229786373347
42-43	22.25100648543696	27.715043971533763	27.71747982183462	22.316469721194657
44-45	22.216318077193396	27.708753365120753	27.805614949297784	22.26931360838807
46-47	22.33290099214162	27.817454926880732	27.685672340643425	22.163971740334222
48-49	22.2575017559788	27.705188823939608	27.72353311296241	22.31377630711918
50-51	22.241603709042305	27.685344173469755	27.78953346464342	22.28351865284452
52-53	22.329777388895526	27.767884759350203	27.70233470013282	22.200003151621452
54-55	22.25733320268114	27.70100717222431	27.707425552937607	22.334234072156946
56-57	22.232792427481414	27.7091991486413	27.78843790711209	22.269570516765192
58-59	22.34420328496025	27.768994335227365	27.710185593286074	22.176616786526306
60-61	22.267722619836462	27.696814949127308	27.715479995642607	22.319982435393626
62-63	22.237803898300605	27.68013037157287	27.788140986011694	22.29392474411483
64-65	22.349336861766055	27.791773329368546	27.660542776876284	22.198347031989115
66-67	22.264195653129644	27.705382442273223	27.71862940731114	22.311792497285992
68-69	22.237312043469494	27.69902593380728	27.789336502160417	22.27432552056281
70-71	22.35040124184447	27.786206318207444	27.683341428346075	22.18005101160201
72-73	22.26864702961382	27.69446663021742	27.716925142178706	22.319961197990057
74-75	22.23680423295038	27.68011926275044	27.79642000341877	22.286656500880405
76-77	22.34062936891697	27.774959180001286	27.684391774424142	22.2000196766576
78-79	22.272920776069384	27.67476016223542	27.734032653343167	22.318286408352037
80-81	22.238559910586314	27.672101716997627	27.811017444905094	22.27832092751097
82-83	22.355965327723723	27.7668246314912	27.68125129350334	22.19595874728174
84-85	22.283787709924983	27.666584908535864	27.715171945432616	22.33445543610654
86-87	22.251760363131496	27.67263585750439	27.7885764625958	22.287027316768313
88-89	22.365688091106517	27.765115241778553	27.67226575484446	22.196930912270467
90-91	22.299110254558514	27.663723486078695	27.71074817351385	22.326418085848942
92-93	22.264773557813776	27.651436877242148	27.798417246324792	22.28537231861928
94-95	22.393028203296232	27.74382263109041	27.650860861063077	22.21228830455028
96-97	22.299081634911662	27.68297045626452	27.692380433320174	22.32556747550364
98-99	22.26976209583897	27.643721741024486	27.765038868336855	22.32147729479969
100	22.693957864901673	27.188241519014667	27.483826322660626	22.633974293423034
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	11.0
2	6.0
3	5.5
4	8.0
5	9.0
6	28.0
7	67.0
8	140.5
9	301.0
10	615.5
11	1221.5
12	2132.0
13	3325.5
14	4982.5
15	7707.0
16	11266.5
17	16286.0
18	24329.5
19	35588.5
20	50274.5
21	70271.5
22	98096.0
23	135433.5
24	183525.0
25	243098.5
26	312197.5
27	389795.0
28	473995.5
29	561849.0
30	654389.0
31	748826.0
32	848553.5
33	956545.5
34	1063865.5
35	1177331.5
36	1301937.5
37	1433158.5
38	1571476.5
39	1714054.0
40	1853714.5
41	1985961.0
42	2110142.0
43	2215003.0
44	2288939.5
45	2336099.0
46	2357802.5
47	2339230.0
48	2281335.5
49	2191846.0
50	2073670.0
51	1930704.5
52	1765787.5
53	1590780.5
54	1413628.0
55	1233038.0
56	1055173.5
57	891281.5
58	745294.0
59	616791.5
60	505795.5
61	412672.5
62	334593.5
63	269364.5
64	215289.0
65	172616.0
66	138250.5
67	109210.0
68	86280.5
69	67046.0
70	51232.0
71	38768.0
72	28467.0
73	20230.0
74	14144.5
75	9809.0
76	6665.5
77	4483.5
78	2945.0
79	1936.5
80	1252.5
81	773.5
82	508.5
83	329.5
84	198.5
85	125.0
86	80.0
87	54.0
88	37.0
89	23.0
90	15.0
91	12.5
92	10.5
93	10.0
94	10.0
95	7.0
96	4.0
97	2.0
98	0.5
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04013451871788322
2	0.0020385481155599503
3	7.656609289283825E-4
4	0.059833412239481465
5	0.04674196038667072
6	0.31474642891499793
7	0.03083087055377613
8	0.0027907087258422403
9	0.2373799599881413
10-11	0.07833849186937523
12-13	0.12645941337489683
14-15	8.601631594510291E-4
16-17	0.01912609428761403
18-19	0.014608309083546683
20-21	0.17169223099862937
22-23	0.22023841254150286
24-25	0.03964079277882613
26-27	0.03901784950415644
28-29	0.015309361344668766
30-31	6.1040726449832E-4
32-33	0.03136413314029678
34-35	0.27691564314322287
36-37	0.04857703941406457
38-39	0.20082977587079562
40-41	0.0904251342915268
42-43	2.912211593657072E-4
44-45	0.014614094934395009
46-47	0.1494195981580011
48-49	0.12751822408014038
50-51	0.03295234919816207
52-53	0.06948710437991223
54-55	0.10428899723258897
56-57	0.03235640656078457
58-59	0.24990246984086673
60-61	0.01491881641240681
62-63	0.015502223039612944
64-65	0.07622087266390118
66-67	0.045632043092401625
68-69	0.06106483710596703
70-71	0.027349719069919003
72-73	0.05906390205046917
74-75	0.014345056189882103
76-77	0.014373987807182395
78-79	0.18429874409836838
80-81	0.0015101089499249126
82-83	0.046538663179817204
84-85	0.03170574537922922
86-87	0.16080433915013526
88-89	0.03130304948848092
90-91	0.03365366612756961
92-93	0.10594369624447404
94-95	0.12120304452603607
96-97	0.07943300440078413
98-99	0.011474797862307757
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
63	1.0
64	1.0
65	0.0
66	0.0
67	0.0
68	1.0
69	1.0
70	0.0
71	2.0
72	2.0
73	2.0
74	4.0
75	5.0
76	3.0
77	6.0
78	5.0
79	17.0
80	29.0
81	65.0
82	95.0
83	115.0
84	100.0
85	121.0
86	168.0
87	181.0
88	246.0
89	436.0
90	575.0
91	855.0
92	1426.0
93	2482.0
94	5154.0
95	10461.0
96	24152.0
97	68694.0
98	270371.0
99	1846266.0
100	4.9618586E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.33728157203784
#Duplication Level	Percentage of total
1	74.5313223044835
2	12.826389069385996
3	6.348792100559033
4	3.0180873624790676
5	1.3936135661531799
6	0.7040071511700776
7	0.3880298673120496
8	0.18514600110238605
9	0.12589980396460068
>10	0.47276210458263085
>50	0.0042531968171418185
>100	0.0
>500	0.0016974719903361097
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.857233898883539E-6	5.7858508483253085E-6	5.7858508483253085E-6	2.3143403393301234E-5	2.1214786443859465E-5	3.471510508995185E-5
2	3.857233898883539E-6	7.714467797767079E-6	1.5428935595534157E-5	4.050095593827716E-5	2.7000637292184772E-5	5.2072657634927775E-5
3	3.857233898883539E-6	1.3500318646092386E-5	1.5428935595534157E-5	5.978712543269486E-5	4.050095593827716E-5	5.5929891533811314E-5
4	3.857233898883539E-6	1.7357552544975926E-5	1.7357552544975926E-5	8.293052882599609E-5	5.5929891533811314E-5	5.785850848325308E-5
5	3.857233898883539E-6	1.7357552544975926E-5	1.9286169494417695E-5	9.835946442153024E-5	7.521606102822901E-5	5.785850848325308E-5
6	7.714467797767079E-6	1.7357552544975926E-5	2.3143403393301234E-5	1.1764563391594795E-4	8.293052882599609E-5	6.36443593315784E-5
7	7.714467797767079E-6	1.9286169494417695E-5	2.7000637292184772E-5	1.3500318646092387E-4	1.0414531526985555E-4	6.557297628102017E-5
8	9.643084747208848E-6	1.9286169494417695E-5	3.278648814051008E-5	1.5236073900589978E-4	1.2150286781483149E-4	6.94302101799037E-5
9	1.1571701696650617E-5	2.5072020342743003E-5	4.050095593827716E-5	1.6393244070255042E-4	1.446462712081327E-4	9.643084747208847E-5
10-11	1.639324407025504E-5	2.8929254241626545E-5	4.532249831188158E-5	1.9189738646945606E-4	1.6875398307615483E-4	1.0896685764345998E-4
12-13	2.5072020342743003E-5	3.278648814051008E-5	4.917973221076512E-5	2.3046972545829144E-4	1.9575462036833962E-4	1.3114595256204033E-4
14-15	2.989356271634743E-5	3.471510508995185E-5	5.496558305909043E-5	2.690420644471268E-4	2.2854110850884968E-4	1.4946781358173713E-4
16-17	3.18221796657892E-5	3.567941356467274E-5	6.26800508568575E-5	3.0761440343596226E-4	2.487915864779883E-4	1.6682536612671307E-4
18-19	3.278648814051008E-5	3.857233898883539E-5	6.653728475574104E-5	3.4329381700063495E-4	2.690420644471268E-4	1.909330779947352E-4
20-21	3.664372203939362E-5	4.242957288771893E-5	8.87163796743214E-5	3.780089220905868E-4	2.960427017393116E-4	2.1311217291331553E-4
22-23	3.857233898883539E-5	4.242957288771893E-5	9.739515594680935E-5	4.146526441299805E-4	3.2593626445565906E-4	2.3143403393301236E-4
24-25	3.857233898883539E-5	4.43581898371607E-5	1.0703824069401821E-4	4.503320576946532E-4	3.4329381700063495E-4	2.5168451190215094E-4
26-27	3.857233898883539E-5	4.917973221076512E-5	1.1185978306762263E-4	4.908330136329304E-4	3.712587627675406E-4	2.8157807461849833E-4
28-29	4.3393881362439814E-5	5.2072657634927775E-5	1.2246717628955236E-4	5.380841288942536E-4	3.944021661608419E-4	3.047214780117996E-4
30-31	4.821542373604424E-5	5.5929891533811314E-5	1.2343148476427326E-4	5.843709356808562E-4	4.2622434582663106E-4	3.114716373348458E-4
32-33	5.110834916020689E-5	5.882281695797397E-5	1.272887186631568E-4	6.084786475488782E-4	4.5901083396714115E-4	3.2690057293037993E-4
34-35	5.303696610964866E-5	6.171574238213663E-5	1.3211026103676122E-4	6.422294441641093E-4	4.811899288857215E-4	3.4811535937423943E-4
36-37	5.496558305909043E-5	6.653728475574104E-5	1.398247288345283E-4	6.750159323046193E-4	5.159050339756733E-4	3.799375390400286E-4
38-39	5.785850848325308E-5	6.94302101799037E-5	1.5332504748062067E-4	7.058738034956876E-4	5.409770543184163E-4	4.069381763322134E-4
40-41	5.785850848325308E-5	7.039451865462458E-5	1.6200382375310867E-4	7.405889085856396E-4	5.69906308560043E-4	4.310458882002355E-4
42-43	5.785850848325308E-5	7.907329492711255E-5	1.7164690850031747E-4	7.675895458778242E-4	5.969069458522276E-4	4.541892915935367E-4
44-45	5.785850848325308E-5	8.389483730071698E-5	1.7936137629808458E-4	8.090548102908223E-4	6.239075831444125E-4	4.802256204110006E-4
46-47	5.978712543269486E-5	8.582345425015874E-5	1.8707584409585165E-4	8.418412984313325E-4	6.412651356893883E-4	5.081905661779063E-4
48-49	6.171574238213663E-5	9.064499662376317E-5	1.9961185426722313E-4	8.775207119960051E-4	6.595869967090853E-4	5.255481187228823E-4
50-51	7.039451865462458E-5	9.353792204792581E-5	2.1407648138803642E-4	9.151287425101197E-4	6.885162509507117E-4	5.400127458436955E-4
52-53	7.425175255350812E-5	9.45022305226467E-5	2.2371956613524525E-4	9.469509221759088E-4	7.28052898414268E-4	5.727992339842055E-4
54-55	7.810898645239166E-5	9.643084747208847E-5	2.34326959357175E-4	9.922734204877905E-4	7.579464611306155E-4	6.075143390741574E-4
56-57	8.389483730071698E-5	1.0414531526985555E-4	2.4493435257910473E-4	0.0010269885255777423	7.907329492711255E-4	6.509082204365973E-4
58-59	8.775207119960052E-5	1.0800254916873909E-4	2.545774373263136E-4	0.0010597750137182524	8.273766713105191E-4	6.981593356979205E-4
60-61	8.968068814904227E-5	1.0800254916873909E-4	2.603632881746389E-4	0.0010915971933840416	8.591988509763083E-4	7.357673662120351E-4
62-63	9.45022305226467E-5	1.128240915423435E-4	2.7000637292184774E-4	0.0011224550645751099	8.919853391168185E-4	7.685538543525451E-4
64-65	9.643084747208847E-5	1.1475270849178529E-4	2.7675653224489396E-4	0.0011610274035639453	9.295933696309329E-4	8.11947735714985E-4
66-67	9.643084747208847E-5	1.1860994239066883E-4	2.835066915679401E-4	0.001195742508653897	9.62379857771443E-4	8.62091776400471E-4
68-69	9.739515594680935E-5	1.2053855934011061E-4	2.8929254241626546E-4	0.0012275646883196864	9.922734204877905E-4	8.919853391168185E-4
70-71	1.0703824069401821E-4	1.2343148476427326E-4	3.018285525876369E-4	0.0012593868679854755	0.0010250599086283007	9.257361357320494E-4
72-73	1.1185978306762263E-4	1.25360101713715E-4	3.143645627590084E-4	0.0013018164408731946	0.0010597750137182524	9.71058634043931E-4
74-75	1.137884000170644E-4	1.25360101713715E-4	3.230433390314964E-4	0.001338460162912588	0.001105097512030134	0.0010067380476086038
76-77	1.1571701696650617E-4	1.311459525620403E-4	3.2786488140510085E-4	0.0013731752680025398	0.0011436698510189692	0.0010347029933755092
78-79	1.1571701696650617E-4	1.388604203598074E-4	3.394365831017515E-4	0.0014117476069913752	0.0011909209662802926	0.0010752039493137865
80-81	1.1668132544122706E-4	1.388604203598074E-4	3.539012102225647E-4	0.0014445340951318854	0.0012323862306932907	0.0011147405967773427
82-83	1.2246717628955236E-4	1.4078903730924917E-4	3.6065136954561094E-4	0.0014782848917471163	0.001279637345954614	0.001148491393392574
84-85	1.2825302713787766E-4	1.436819627334118E-4	3.693301458180989E-4	0.0015216787731095562	0.0013211026103676121	0.0011870637323814093
86-87	1.3596749493564474E-4	1.4850350510701624E-4	3.7704461361586595E-4	0.0015679655798961586	0.001356782023932285	0.0012555296340865918
88-89	1.3789611188508652E-4	1.5236073900589978E-4	3.866876983630748E-4	0.0015911089832894598	0.0014001759052947246	0.001306637983246799
>>END_MODULE
