##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674152
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27017640
Total Bases	2.6 Gbp
Sequences flagged as poor quality	0
Sequence length	62-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.71804184229266	37.0	35.0	37.0	29.0	37.0
2	34.189060443473224	37.0	34.0	37.0	27.0	37.0
3	34.4402142822245	37.0	34.0	37.0	28.0	37.0
4	34.44556482357452	37.0	34.0	37.0	28.0	37.0
5	34.428691477123834	37.0	34.0	37.0	28.0	37.0
6	34.33886031496459	37.0	34.0	37.0	28.0	37.0
7	34.43389000667712	37.0	34.0	37.0	28.0	37.0
8	34.38932704706999	37.0	34.0	37.0	28.0	37.0
9	34.376762441131056	37.0	34.0	37.0	28.0	37.0
10-11	34.376648441536716	37.0	34.0	37.0	27.5	37.0
12-13	34.35812265616094	37.0	34.0	37.0	28.0	37.0
14-15	34.416910877485975	37.0	34.0	37.0	28.0	37.0
16-17	34.357155491745395	37.0	34.0	37.0	28.0	37.0
18-19	34.384191346838584	37.0	34.0	37.0	28.0	37.0
20-21	34.30527734842865	37.0	34.0	37.0	27.5	37.0
22-23	34.24839023689708	37.0	34.0	37.0	27.0	37.0
24-25	34.34432715070598	37.0	34.0	37.0	27.5	37.0
26-27	34.32194605080237	37.0	34.0	37.0	27.0	37.0
28-29	34.363259651842284	37.0	34.0	37.0	28.0	37.0
30-31	34.37237081032984	37.0	34.0	37.0	28.0	37.0
32-33	34.34145717390564	37.0	34.0	37.0	27.5	37.0
34-35	34.21024802684468	37.0	34.0	37.0	27.0	37.0
36-37	34.3433283032863	37.0	34.0	37.0	27.5	37.0
38-39	34.293316792288294	37.0	34.0	37.0	27.5	37.0
40-41	34.312064469731624	37.0	34.0	37.0	27.0	37.0
42-43	34.32293581156607	37.0	34.0	37.0	27.0	37.0
44-45	34.31381821284168	37.0	34.0	37.0	27.0	37.0
46-47	34.22704253591357	37.0	34.0	37.0	27.0	37.0
48-49	34.261347068063685	37.0	34.0	37.0	27.0	37.0
50-51	34.28437724390435	37.0	34.0	37.0	27.0	37.0
52-53	34.24134824507248	37.0	34.0	37.0	27.0	37.0
54-55	34.1768063753903	37.0	34.0	37.0	27.0	37.0
56-57	34.21057055686581	37.0	34.0	37.0	27.0	37.0
58-59	34.06712971599296	37.0	34.0	37.0	26.5	37.0
60-61	34.146244435117204	37.0	34.0	37.0	27.0	37.0
62-63	34.1764389709817	37.0	34.0	37.0	27.0	37.0
64-65	34.11251703007802	37.0	34.0	37.0	27.0	37.0
66-67	34.166808934719896	37.0	34.0	37.0	27.0	37.0
68-69	34.08139723548618	37.0	34.0	37.0	27.0	37.0
70-71	34.08611730500773	37.0	34.0	37.0	27.0	37.0
72-73	34.106352552966094	37.0	34.0	37.0	27.0	37.0
74-75	34.08193826979952	37.0	34.0	37.0	27.0	37.0
76-77	34.05917244323493	37.0	34.0	37.0	27.0	37.0
78-79	33.98084877940326	37.0	34.0	37.0	26.0	37.0
80-81	33.997558741977485	37.0	34.0	37.0	26.0	37.0
82-83	33.931397924624164	37.0	33.5	37.0	26.0	37.0
84-85	33.8976286284374	37.0	33.0	37.0	26.0	37.0
86-87	33.82385874107375	37.0	33.0	37.0	26.0	37.0
88-89	33.826172593151426	36.5	33.0	37.0	26.0	37.0
90-91	33.80061537934448	36.5	33.0	37.0	26.0	37.0
92-93	33.75056040308055	36.0	33.0	37.0	26.0	37.0
94-95	33.64160685779619	36.0	33.0	37.0	25.0	37.0
96-97	33.740585210275185	36.0	33.0	37.0	26.0	37.0
98-99	33.78649507154129	36.0	33.0	37.0	25.5	37.0
100	34.57075928306545	37.0	34.0	37.0	28.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	285.0
20	13537.0
21	69534.0
22	126721.0
23	179439.0
24	230776.0
25	283654.0
26	349947.0
27	434834.0
28	545117.0
29	687869.0
30	873887.0
31	1127984.0
32	1495708.0
33	2093918.0
34	3204703.0
35	5872473.0
36	9076223.0
37	351031.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.951232877178942	35.736268022982074	17.472949907136705	24.839549192702286
2	25.010892984917714	29.31845225008428	30.680959980291213	14.989694784706797
3	16.177076790836736	28.383797284056016	32.21348431427554	23.22564161083171
4	18.156385887088426	29.697168125713198	30.265565488576346	21.880880498622034
5	20.926590258244705	28.27407154908454	29.692759717600826	21.106578475069934
6	20.525412230344383	27.125534479746733	30.377679190984875	21.971374098924016
7	21.706794369999812	28.806024690776788	28.450271654212017	21.036909285011383
8	19.29252190472087	26.938371484833585	31.20627157238251	22.562835038063035
9	20.62595744611415	26.08472238271855	30.990862809978164	22.298457361189133
10-11	21.690363819135396	27.463049514857925	28.73243767312609	22.11414899288059
12-13	21.752219500266413	27.665777301370113	28.2542102606527	22.327792937710772
14-15	21.69354278139491	27.74969795415714	28.384136724814084	22.17262253963387
16-17	21.89095596108695	27.847804908821523	28.24306538892458	22.01817374116695
18-19	21.81963773856421	27.56546567487732	28.229381994492865	22.385514592065604
20-21	21.82220457133218	27.585822495243907	28.370393808518497	22.221579124905418
22-23	21.998339451842412	27.933047027916135	27.992330312108738	22.076283208132715
24-25	22.00205773588358	27.649581210364715	27.959806178736475	22.388554875015238
26-27	21.945648964010925	27.57540711358249	28.23683597404515	22.24210794836144
28-29	22.118287707958906	27.793678348403255	27.931763550156468	22.15627039348137
30-31	22.094784782367626	27.546764684501795	27.910552519569244	22.44789801356134
32-33	22.01904420261765	27.53265674443081	28.125499296490663	22.322799756460874
34-35	22.1776585199766	27.77976110443705	27.84669698712614	22.195883388460206
36-37	22.169330355550464	27.537874670012513	27.815001503323387	22.477793471113635
38-39	22.07768548060367	27.49091517412538	28.073755757902795	22.357643587368152
40-41	22.2172915154008	27.701939433090274	27.841799503025488	22.23896954848344
42-43	22.1768164675337	27.488051679688137	27.799114873709357	22.53601697906881
44-45	22.083742022475256	27.48485605087817	28.049107592464008	22.38229433418257
46-47	22.207338017683494	27.71342257973151	27.82383087723875	22.255408525346247
48-49	22.19764980255483	27.464024911961037	27.798140093514316	22.54018519196981
50-51	22.09634855932331	27.456106569435345	28.032316160236576	22.41522871100476
52-53	22.2317972942583	27.662651560660116	27.809254024583467	22.296297120498114
54-55	22.208404071336375	27.438838667985028	27.778192060803637	22.574565199874964
56-57	22.11430927397122	27.46489910097064	28.015156455939543	22.405635169118597
58-59	22.2425222839356	27.64351921627452	27.83712826435715	22.27683023543273
60-61	22.23539425603609	27.439467870894035	27.76259094476868	22.56254692830119
62-63	22.12165937452311	27.44541459582781	28.005341958283008	22.42758407136607
64-65	22.256906446053364	27.666596241481017	27.76330876232259	22.313188550143025
66-67	22.239889881573628	27.4492486518685	27.755932542281414	22.554928924276457
68-69	22.121021889558055	27.45732353714064	27.99667607479891	22.424978498502394
70-71	22.287528883559894	27.67597517079745	27.748878198847372	22.28761774679528
72-73	22.229230215122623	27.455607348851963	27.74249529618187	22.57266713984355
74-75	22.13836768790607	27.43233149599841	27.991209875115437	22.438090940980086
76-77	22.270835723806	27.655549595171546	27.765929506749522	22.307685174272933
78-79	22.23407121580793	27.45033890588565	27.7481114330573	22.56747844524912
80-81	22.141333321044726	27.435167735710824	27.99772505286184	22.425773890382615
82-83	22.271722700338795	27.65563022168812	27.762422222572823	22.31022485540026
84-85	22.256266162483758	27.423345499865015	27.738194451052912	22.58219388659832
86-87	22.15765421006758	27.438752439475746	27.968179970642094	22.435413379814587
88-89	22.28318154804333	27.644678010912248	27.759569508652593	22.312570932391825
90-91	22.267618570489738	27.403962766041122	27.757932259394035	22.570486404075105
92-93	22.173183854766947	27.40680057891463	27.993764131949423	22.426251434369004
94-95	22.317305175799692	27.617395344123906	27.747325278810344	22.317974201266065
96-97	22.282648233700886	27.39494402249768	27.744458923655458	22.577948820145973
98-99	22.171473226028205	27.330521723374517	27.980910408879843	22.517094641717435
100	22.853568209306633	26.732992003637467	27.390244982109003	23.023194804946893
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	5.0
2	1.5
3	1.5
4	1.5
5	1.5
6	2.0
7	2.0
8	4.5
9	18.5
10	47.5
11	97.5
12	176.0
13	381.0
14	734.0
15	1391.0
16	2632.0
17	4655.0
18	7627.0
19	12216.0
20	19786.0
21	31189.5
22	46688.5
23	67062.5
24	92707.0
25	122060.5
26	155669.5
27	193097.5
28	233485.5
29	279325.5
30	328992.0
31	380717.5
32	434387.0
33	489739.5
34	546561.5
35	604816.0
36	670189.0
37	741314.5
38	812040.5
39	885692.0
40	965222.0
41	1044046.5
42	1116888.5
43	1181249.5
44	1236287.0
45	1279341.5
46	1302459.0
47	1306632.5
48	1286961.0
49	1236127.5
50	1160594.0
51	1066856.0
52	959301.5
53	841322.5
54	717617.5
55	597808.0
56	488988.5
57	396471.5
58	320404.0
59	258474.5
60	209475.0
61	170690.5
62	139794.0
63	115979.5
64	97145.5
65	80469.0
66	66032.0
67	53662.0
68	43210.0
69	34186.0
70	26193.0
71	19607.0
72	14350.5
73	9975.0
74	6900.5
75	4777.5
76	3117.5
77	1985.5
78	1233.5
79	747.0
80	462.5
81	284.5
82	159.5
83	92.0
84	62.0
85	44.5
86	34.5
87	27.0
88	19.0
89	11.0
90	6.0
91	5.5
92	3.5
93	1.5
94	2.5
95	3.0
96	2.5
97	1.0
98	0.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03176813370819953
2	9.327239536835934E-4
3	0.0013583717896899952
4	0.04268692602314636
5	0.060804718694897106
6	0.14200722194832707
7	7.772699614029945E-5
8	0.002764860291276366
9	0.19496891660411494
10-11	0.10066016128721826
12-13	0.1680698240112756
14-15	8.068802456469181E-4
16-17	0.022524173095799634
18-19	0.01868779064344628
20-21	0.14372276779170942
22-23	0.2899698123152133
24-25	0.05066504698411853
26-27	0.051464524658704464
28-29	0.01973525444857508
30-31	9.031136694396698E-4
32-33	0.040310700712571486
34-35	0.29304558059105085
36-37	0.021543332430219664
38-39	0.08272743289199205
40-41	0.05793067048047128
42-43	5.163293315034178E-4
44-45	0.018334317875284445
46-47	0.17337191553370318
48-49	0.0853405404765183
50-51	0.02134716429710367
52-53	0.039198464410659106
54-55	0.13554847869762127
56-57	0.04241118025112482
58-59	0.24488445326830915
60-61	0.018589706576888285
62-63	7.64315476191027E-4
64-65	0.09927403353046503
66-67	0.0383120086531619
68-69	0.08084903796640251
70-71	0.03642620694127347
72-73	0.07655740266390078
74-75	0.018676692662715457
76-77	0.018552702648533863
78-79	0.14178521828675844
80-81	0.0027000896933153367
82-83	0.06108058089640384
84-85	0.0404922120780718
86-87	0.21388750902055126
88-89	0.040742326484002
90-91	0.0011326184832750558
92-93	0.13902521851466715
94-95	0.12500504843742025
96-97	0.0010390104723550892
98-99	0.01337153925718668
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62	1.0
63	0.0
64	0.0
65	0.0
66	1.0
67	0.0
68	1.0
69	1.0
70	0.0
71	0.0
72	1.0
73	2.0
74	3.0
75	3.0
76	0.0
77	0.0
78	2.0
79	2.0
80	4.0
81	19.0
82	23.0
83	25.0
84	22.0
85	33.0
86	48.0
87	49.0
88	85.0
89	178.0
90	192.0
91	323.0
92	601.0
93	1266.0
94	2892.0
95	6450.0
96	17145.0
97	51010.0
98	200815.0
99	1276992.0
100	2.5459451E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.93161651988692
#Duplication Level	Percentage of total
1	58.958757375979495
2	15.100203743511445
3	10.024743033861812
4	6.335036099577573
5	3.8418468125615113
6	2.142008531301349
7	1.1723672539435344
8	0.5892905154079527
9	0.33620318650078046
>10	1.4884679627650348
>50	0.011075484589490129
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.70128553049045E-6	0.0	3.70128553049045E-6	0.0	1.48051421219618E-5	3.70128553049045E-5
2	3.70128553049045E-6	3.70128553049045E-6	3.70128553049045E-6	3.70128553049045E-6	4.071414083539495E-5	4.44154263658854E-5
3	7.4025710609809E-6	7.4025710609809E-6	3.70128553049045E-6	7.4025710609809E-6	7.032442507931855E-5	4.44154263658854E-5
4	1.110385659147135E-5	7.4025710609809E-6	3.70128553049045E-6	1.48051421219618E-5	9.253213826226125E-5	5.18179974268663E-5
5	1.110385659147135E-5	1.110385659147135E-5	3.70128553049045E-6	1.850642765245225E-5	1.184411369756944E-4	5.551928295735675E-5
6	1.110385659147135E-5	1.110385659147135E-5	3.70128553049045E-6	1.850642765245225E-5	1.2214242250618485E-4	6.66231395488281E-5
7	1.110385659147135E-5	1.48051421219618E-5	1.110385659147135E-5	2.590899871343315E-5	1.554539922805989E-4	7.032442507931855E-5
8	1.110385659147135E-5	1.48051421219618E-5	1.110385659147135E-5	2.96102842439236E-5	1.776617054635416E-4	7.4025710609809E-5
9	1.110385659147135E-5	1.48051421219618E-5	1.110385659147135E-5	3.331156977441405E-5	2.072719897074652E-4	9.253213826226125E-5
10-11	1.110385659147135E-5	1.48051421219618E-5	1.48051421219618E-5	3.886349807014972E-5	2.2577841735991745E-4	1.0548663761897783E-4
12-13	1.2954499356716575E-5	1.48051421219618E-5	2.0357070417697476E-5	4.996735466162108E-5	2.6279127266482194E-4	1.1659049421044917E-4
14-15	1.48051421219618E-5	1.48051421219618E-5	2.22077131829427E-5	5.92205684878472E-5	2.868496286130099E-4	1.2029177974093963E-4
16-17	1.48051421219618E-5	1.48051421219618E-5	2.22077131829427E-5	7.032442507931856E-5	3.0165477073497166E-4	1.3879820739339188E-4
18-19	1.48051421219618E-5	1.6655784887207026E-5	2.590899871343315E-5	7.772699614029945E-5	3.2756376944840485E-4	1.5730463504584413E-4
20-21	1.48051421219618E-5	1.850642765245225E-5	2.96102842439236E-5	7.957763890554468E-5	3.664272675185545E-4	1.7951234822878681E-4
22-23	1.850642765245225E-5	2.7759641478678376E-5	3.146092700916882E-5	8.512956720128036E-5	3.9603755176247817E-4	1.9061620482025817E-4
24-25	1.850642765245225E-5	2.96102842439236E-5	4.626606913113062E-5	9.623342379275169E-5	4.311997643021374E-4	2.017200614117295E-4
26-27	2.22077131829427E-5	3.331156977441405E-5	4.811671189637585E-5	1.1288920867995873E-4	4.4600490642409925E-4	2.1837584629893656E-4
28-29	3.331156977441405E-5	3.70128553049045E-5	4.811671189637585E-5	1.258437080366753E-4	4.848684044942489E-4	2.368822739513888E-4
30-31	3.70128553049045E-5	3.70128553049045E-5	4.811671189637585E-5	1.258437080366753E-4	5.126280459729273E-4	2.4613548777761493E-4
32-33	4.44154263658854E-5	4.626606913113062E-5	4.996735466162108E-5	1.332462790976562E-4	5.311344736253795E-4	2.664925581953124E-4
34-35	4.626606913113062E-5	4.811671189637585E-5	5.551928295735675E-5	1.350969218629014E-4	5.588941151040579E-4	2.979534852044812E-4
36-37	5.551928295735675E-5	4.811671189637585E-5	5.92205684878472E-5	1.3879820739339188E-4	5.903550421132267E-4	3.090573417959526E-4
38-39	6.292185401833765E-5	4.996735466162108E-5	5.92205684878472E-5	1.406488501586371E-4	6.25517254652886E-4	3.4977148263134756E-4
40-41	6.292185401833765E-5	5.18179974268663E-5	6.292185401833765E-5	1.424994929238823E-4	6.551275388968097E-4	3.719791958142902E-4
42-43	6.292185401833765E-5	5.18179974268663E-5	6.66231395488281E-5	1.5175270675010845E-4	7.050948935584307E-4	3.923362662319877E-4
44-45	6.847378231407332E-5	5.736992572260198E-5	7.032442507931856E-5	1.554539922805989E-4	7.310038922718639E-4	4.052907655887043E-4
46-47	7.957763890554468E-5	5.92205684878472E-5	7.772699614029945E-5	1.5915527781108936E-4	7.550622482200519E-4	4.219465504759113E-4
48-49	8.327892443603512E-5	5.92205684878472E-5	9.438278102750648E-5	1.702591344025607E-4	7.939257462902015E-4	4.3675169259787313E-4
50-51	8.698020996652557E-5	6.107121125309242E-5	9.808406655799693E-5	1.7396041993305114E-4	8.290879588298608E-4	4.8301776172900374E-4
52-53	9.438278102750648E-5	6.292185401833765E-5	1.036359948537326E-4	1.7951234822878684E-4	8.475943864823131E-4	5.033748321467012E-4
54-55	9.993470932324215E-5	6.292185401833765E-5	1.0733728038422305E-4	1.8321363375927726E-4	8.679514569000105E-4	5.255825453296439E-4
56-57	9.993470932324215E-5	6.292185401833765E-5	1.0733728038422305E-4	1.850642765245225E-4	8.957110983786889E-4	5.68147328930284E-4
58-59	9.993470932324215E-5	6.292185401833765E-5	1.110385659147135E-4	1.8691491928976773E-4	9.234707398573672E-4	5.885043993479816E-4
60-61	1.0733728038422305E-4	6.66231395488281E-5	1.1288920867995873E-4	1.9616813311599385E-4	9.475290958055552E-4	6.218159691223956E-4
62-63	1.1473985144520395E-4	7.032442507931856E-5	1.1659049421044917E-4	1.9616813311599385E-4	9.900938794061955E-4	6.717833237840166E-4
64-65	1.1473985144520395E-4	7.4025710609809E-5	1.2029177974093963E-4	1.9801877588123909E-4	0.0010197041636501188	7.013936080279403E-4
66-67	1.184411369756944E-4	7.587635337505422E-5	1.258437080366753E-4	2.0912263247271044E-4	0.0010437625195983068	7.365558205675996E-4
68-69	1.184411369756944E-4	8.512956720128036E-5	1.2954499356716573E-4	2.146745607684461E-4	0.0010752234466074755	7.717180331072588E-4
70-71	1.2029177974093963E-4	8.88308527317708E-5	1.350969218629014E-4	2.1837584629893654E-4	0.001101132445320909	8.14282816707899E-4
72-73	1.258437080366753E-4	9.438278102750648E-5	1.3879820739339188E-4	2.2392777459467222E-4	0.0011307427295648325	8.420424581865773E-4
74-75	1.2954499356716573E-4	9.62334237927517E-5	1.406488501586371E-4	2.3318098842089834E-4	0.0011807100842264535	8.790553134914819E-4
76-77	1.2954499356716573E-4	9.808406655799693E-5	1.48051421219618E-4	2.368822739513888E-4	0.0012010671546441509	9.068149549701602E-4
78-79	1.2954499356716573E-4	1.0178535208848737E-4	1.5175270675010845E-4	2.4613548777761493E-4	0.0012232748678270937	9.419771675098195E-4
80-81	1.3139563633241097E-4	1.0548663761897783E-4	1.5360334951535366E-4	2.4798613054286017E-4	0.0012639890086624888	9.715874517537431E-4
82-83	1.3879820739339188E-4	1.0733728038422305E-4	1.554539922805989E-4	2.5538870160384105E-4	0.0012991512212021479	0.001012301592589138
84-85	1.4435013568912754E-4	1.0733728038422305E-4	1.5730463504584413E-4	2.609406298995767E-4	0.0013380147192722978	0.0010548663761897783
86-87	1.5175270675010845E-4	1.1103856591471349E-4	1.5915527781108934E-4	2.7204448649104806E-4	0.0013861314311686735	0.0010900285887294374
88-89	1.5175270675010845E-4	1.1473985144520395E-4	1.6655784887207025E-4	2.868496286130099E-4	0.0014268455720040684	0.001134444015095323
>>END_MODULE
