##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674154
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	62497936
Total Bases	6.2 Gbp
Sequences flagged as poor quality	0
Sequence length	64-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.13645575431483	37.0	35.0	37.0	30.0	37.0
2	34.75043084622827	37.0	35.0	37.0	29.0	37.0
3	34.76226781313226	37.0	35.0	37.0	29.0	37.0
4	34.79240882450902	37.0	35.0	37.0	29.0	37.0
5	34.799685240805395	37.0	35.0	37.0	29.0	37.0
6	34.660824319062314	37.0	35.0	37.0	29.0	37.0
7	34.79880594776762	37.0	35.0	37.0	29.0	37.0
8	34.7664748000638	37.0	35.0	37.0	29.0	37.0
9	34.7316900193312	37.0	35.0	37.0	29.0	37.0
10-11	34.72226130795744	37.0	35.0	37.0	29.0	37.0
12-13	34.63428319456822	37.0	35.0	37.0	29.0	37.0
14-15	34.6699806534411	37.0	35.0	37.0	29.0	37.0
16-17	34.59989615497062	37.0	35.0	37.0	28.0	37.0
18-19	34.55661099752158	37.0	35.0	37.0	28.0	37.0
20-21	34.54362656872381	37.0	35.0	37.0	28.0	37.0
22-23	34.43029562608275	37.0	35.0	37.0	28.0	37.0
24-25	34.4148790849669	37.0	35.0	37.0	28.0	37.0
26-27	34.38250456783085	37.0	35.0	37.0	27.5	37.0
28-29	34.34417058988956	37.0	35.0	37.0	27.5	37.0
30-31	34.25348069094634	37.0	34.0	37.0	27.0	37.0
32-33	34.19982315896	37.0	34.0	37.0	27.0	37.0
34-35	34.16482016302106	37.0	34.0	37.0	27.0	37.0
36-37	34.10581486243002	37.0	34.0	37.0	27.0	37.0
38-39	34.08696841604497	37.0	34.0	37.0	27.0	37.0
40-41	34.002530723574615	37.0	34.0	37.0	26.0	37.0
42-43	33.948696273745746	37.0	34.0	37.0	26.0	37.0
44-45	33.869539219663196	37.0	34.0	37.0	26.0	37.0
46-47	33.78415112780685	37.0	34.0	37.0	25.5	37.0
48-49	33.82995392839853	37.0	34.0	37.0	26.0	37.0
50-51	33.740614458051866	37.0	34.0	37.0	25.0	37.0
52-53	33.63281098595	37.0	33.0	37.0	25.0	37.0
54-55	33.54102038665725	37.0	33.0	37.0	24.5	37.0
56-57	33.49478452376411	37.0	33.0	37.0	24.5	37.0
58-59	33.39682287267854	37.0	33.0	37.0	24.0	37.0
60-61	33.29866083897555	37.0	33.0	37.0	23.5	37.0
62-63	33.23260379350768	37.0	33.0	37.0	23.0	37.0
64-65	33.19462844726341	37.0	33.0	37.0	23.0	37.0
66-67	33.12039557368034	36.0	33.0	37.0	23.0	37.0
68-69	33.07958167714014	36.0	32.5	37.0	23.0	37.0
70-71	32.94106869190523	36.0	32.0	37.0	22.0	37.0
72-73	32.82422912644568	36.0	32.0	37.0	21.5	37.0
74-75	32.72039921748651	36.0	32.0	37.0	21.0	37.0
76-77	32.72698810598004	36.0	32.0	37.0	21.0	37.0
78-79	32.66059581879212	36.0	32.0	37.0	21.0	37.0
80-81	32.55290542027603	36.0	31.5	37.0	20.5	37.0
82-83	32.445790163449594	36.0	31.0	37.0	20.0	37.0
84-85	32.337649337252984	36.0	31.0	37.0	20.0	37.0
86-87	32.20468054972634	36.0	31.0	37.0	19.5	37.0
88-89	32.094126923296656	36.0	31.0	37.0	19.0	37.0
90-91	31.988545546962584	36.0	30.0	37.0	18.5	37.0
92-93	31.93579619843048	36.0	30.0	37.0	18.0	37.0
94-95	31.83824618627318	36.0	30.0	37.0	18.0	37.0
96-97	31.81733840134479	36.0	30.0	37.0	18.0	37.0
98-99	32.13077072074519	36.0	30.5	37.0	19.5	37.0
100	34.09384325691063	36.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	169.0
20	13332.0
21	137315.0
22	457202.0
23	778895.0
24	965471.0
25	1113215.0
26	1280943.0
27	1485471.0
28	1740414.0
29	2064223.0
30	2475467.0
31	3019887.0
32	3797285.0
33	5040843.0
34	7348050.0
35	1.2513481E7
36	1.7737119E7
37	529154.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	25.93911033605909	26.59920851052464	26.249463137914734	21.212218015501534
2	26.97857771513349	32.30807395046599	23.152567399279057	17.56078093512146
3	17.535527903496263	28.18940472514609	29.62966718609393	24.645400185263725
4	19.89698754439656	29.863884574382922	28.52775362566992	21.7113742555506
5	21.7947489451128	27.422419333781615	28.844513410976326	21.938318310129258
6	21.677132358670637	27.999814697784085	28.064349548143724	22.258703395401554
7	22.02466498527966	28.386167033944794	28.149682664816712	21.43948531595884
8	21.06190871418701	27.378924627188294	29.00506848933386	22.55409816929084
9	21.424672807628394	26.90153661198004	29.154212192271146	22.519578388120426
10-11	22.213087148895628	27.497372292663197	28.049832430515952	22.239708127925223
12-13	22.128237700400806	27.70443447813345	27.863545624725727	22.30378219674002
14-15	22.238236373288117	27.710754506271222	27.69669812257109	22.354310997869568
16-17	22.368683238088597	27.690806523110478	27.724647349861016	22.21586288893991
18-19	22.258258513662913	27.617266966146286	27.722662531421438	22.401811988769367
20-21	22.34872887312441	27.493343079856043	27.71765666871837	22.440271378301173
22-23	22.423717912194036	27.684422863316705	27.658088876201642	22.233770348287617
24-25	22.31887559302026	27.632938539847775	27.706312774118093	22.341873093013874
26-27	22.392681374525548	27.516651209577514	27.672496409315844	22.418171006581098
28-29	22.519078600330587	27.559708958446432	27.616961472666386	22.304250968556595
30-31	22.42233961647026	27.533210459355423	27.621679691434785	22.42277023273953
32-33	22.47044001814521	27.456025503154947	27.593858941635457	22.479675537064388
34-35	22.53760530171065	27.5725909341406	27.577463453384933	22.31234031076382
36-37	22.472847751146556	27.52227555885471	27.59179189659039	22.413084793408345
38-39	22.517397886987418	27.42784919100257	27.556357686914968	22.498395235095046
40-41	22.6232045357802	27.48437780931467	27.518638642288156	22.373779012616975
42-43	22.507578550404272	27.454232635443944	27.55528264139399	22.48290617275779
44-45	22.544151942957516	27.385286380263878	27.54173089232175	22.52883078445685
46-47	22.62460131806148	27.45455844306324	27.55277990572978	22.368060333145497
48-49	22.556203463268716	27.428005806367644	27.544311732011025	22.471478998352616
50-51	22.613823215322988	27.32810378095632	27.500949665731746	22.557123337988948
52-53	22.699146651250963	27.384480375641434	27.479766549514075	22.436606423593528
54-55	22.60966362606069	27.375228163906186	27.50228247947401	22.512825730559115
56-57	22.642630686991502	27.28645827211098	27.502139670052912	22.568771370844605
58-59	22.75768677762428	27.344569330659567	27.481165612856685	22.41657827885947
60-61	22.6811260825805	27.31036646674358	27.471943184991638	22.536564265684284
62-63	22.73601405782156	27.206557699098845	27.456720365482095	22.600707877597504
64-65	22.808790467980298	27.30997184973228	27.433536864073393	22.447700818214027
66-67	22.715150679712856	27.29332309164133	27.46487423447975	22.526651994166063
68-69	22.763489132717872	27.211019114013947	27.441904399923985	22.583587353344203
70-71	22.869761523184376	27.266817076015155	27.40212371201322	22.461297688787248
72-73	22.81426408922203	27.237862667654927	27.390876914525535	22.556996328597506
74-75	22.864643729728854	27.144248878081083	27.37509149479564	22.616015897394426
76-77	22.917761552271003	27.2507569020056	27.36692370945542	22.46455783626797
78-79	22.842771476548513	27.224393880033045	27.39567642303598	22.537158220382462
80-81	22.916199889857346	27.12446060820391	27.343044595675643	22.616294906263107
82-83	23.013352219916765	27.202277414903538	27.298839655717334	22.48553070946237
84-85	22.945452675584647	27.161542698688272	27.30597967113099	22.587024954596085
86-87	23.013377793758135	27.06208825585985	27.28514406124633	22.639389889135682
88-89	23.116864061342472	27.15222856564929	27.209530152202575	22.52137722080566
90-91	23.044221396947513	27.11347538248678	27.250713383521713	22.591589837043994
92-93	23.104597510926258	27.03140713988387	27.1993777035416	22.66461764564827
94-95	23.200440218450595	27.105919852437875	27.15256425845246	22.541075670659065
96-97	23.099522578298576	27.099782358290582	27.177217953877893	22.62347710953295
98-99	23.067974119900537	27.00315936596623	27.200760692452175	22.728105821681062
100	23.255095635276028	26.608401593304066	27.230288944065645	22.90621382735426
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	22.0
2	10.0
3	9.5
4	12.5
5	21.0
6	44.0
7	92.0
8	194.0
9	393.0
10	795.5
11	1453.0
12	2531.0
13	4279.5
14	6947.5
15	10913.5
16	16608.0
17	24521.5
18	35627.5
19	50975.0
20	71458.5
21	99267.5
22	135954.0
23	181307.5
24	236311.5
25	301516.0
26	375616.0
27	457813.5
28	545960.5
29	639686.0
30	743107.0
31	854403.5
32	972540.0
33	1104999.5
34	1253776.5
35	1409826.0
36	1575298.0
37	1751622.0
38	1929244.0
39	2105191.5
40	2275119.5
41	2435425.5
42	2572615.0
43	2664724.5
44	2712541.0
45	2723727.0
46	2691926.0
47	2609527.5
48	2488445.5
49	2349088.5
50	2192054.5
51	2020607.5
52	1847938.5
53	1674997.5
54	1505312.5
55	1348642.0
56	1206549.0
57	1080042.5
58	970114.0
59	871664.0
60	782634.5
61	704066.5
62	631045.5
63	562640.0
64	497623.0
65	434986.5
66	374931.5
67	316931.0
68	262757.5
69	213823.5
70	170440.5
71	133027.0
72	101389.0
73	75597.0
74	55708.5
75	39917.5
76	27948.5
77	19205.0
78	12962.0
79	8594.5
80	5758.5
81	3756.5
82	2325.5
83	1490.0
84	942.0
85	560.5
86	358.0
87	215.0
88	115.5
89	59.5
90	32.5
91	20.5
92	15.0
93	7.5
94	6.0
95	8.0
96	6.0
97	4.0
98	3.5
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07532088739698539
2	0.07563129764797352
3	0.11746147904788408
4	0.05787231117520425
5	0.028853752866334657
6	0.28500141188662614
7	0.05064327244342918
8	0.021189499762040143
9	0.06099561431916727
10-11	0.02742250560082496
12-13	0.08767249529648467
14-15	0.050651272707629896
16-17	0.039330098837184004
18-19	0.06460133339443401
20-21	0.029148162588921334
22-23	0.08323154863866224
24-25	0.07279520398881653
26-27	0.031348235244120705
28-29	0.07584810480781318
30-31	0.04692234956367199
32-33	0.017475777120063613
34-35	0.023773585098874304
36-37	0.019049429088346212
38-39	0.07801457635336949
40-41	0.04349583640650149
42-43	0.017683783989282463
44-45	0.12435850681532908
46-47	0.07067833408130471
48-49	0.003856127344749433
50-51	0.02046787593113475
52-53	0.006535415825572224
54-55	0.04574551069974535
56-57	0.019601447318196234
58-59	0.050787277199042226
60-61	0.06547096211305282
62-63	0.07931301923314715
64-65	0.019529445409111883
66-67	0.06007639069128195
68-69	0.01767658756977082
70-71	0.025211243063968058
72-73	0.02111910841937282
74-75	0.10242984901787613
76-77	0.027263324117634293
78-79	0.030753853750044892
80-81	0.02724255239963189
82-83	0.03352920329604789
84-85	0.03787743328207539
86-87	0.0419313819160059
88-89	0.04318404387141077
90-91	0.05431109019948608
92-93	0.05398422670231963
94-95	0.06203277775589439
96-97	0.04226671538870995
98-99	0.054951551956044675
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
64	3.0
65	3.0
66	2.0
67	4.0
68	3.0
69	9.0
70	4.0
71	3.0
72	3.0
73	3.0
74	7.0
75	7.0
76	7.0
77	11.0
78	17.0
79	19.0
80	32.0
81	23.0
82	38.0
83	63.0
84	80.0
85	148.0
86	206.0
87	434.0
88	688.0
89	1228.0
90	2418.0
91	4756.0
92	9302.0
93	18868.0
94	39953.0
95	89678.0
96	209660.0
97	518832.0
98	1464703.0
99	5778484.0
100	5.4358237E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.62799023500926
#Duplication Level	Percentage of total
1	58.06559454889618
2	17.95694871572577
3	8.653540004142894
4	5.129206255312761
5	3.0471248113674085
6	1.8150990143064636
7	1.1220478533567413
8	0.8246325421235209
9	0.5538811103371815
>10	2.6432038183495705
>50	0.14572098895553676
>100	0.04300033712594033
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	3.200105680289986E-6	4.800158520434979E-6	2.720089828246488E-5	5.440179656492976E-5
2	1.600052840144993E-6	6.400211360579972E-6	3.200105680289986E-6	9.600317040869958E-6	8.800290620797462E-5	6.400211360579972E-5
3	3.200105680289986E-6	9.600317040869958E-6	6.400211360579972E-6	1.7600581241594922E-5	1.0720354028971452E-4	6.88022721262347E-5
4	4.800158520434979E-6	1.120036988101495E-5	8.000264200724965E-6	2.8800951122609873E-5	1.376045442524694E-4	8.160269484739464E-5
5	6.400211360579972E-6	1.9200634081739916E-5	9.600317040869958E-6	3.360110964304485E-5	1.5200501981377432E-4	9.280306472840959E-5
6	8.000264200724965E-6	2.0800686921884907E-5	1.120036988101495E-5	4.0001321003624823E-5	1.6640549537507926E-4	9.920327608898957E-5
7	9.600317040869958E-6	2.4000792602174893E-5	1.4400475561304936E-5	4.0001321003624823E-5	1.824060237765292E-4	1.0560348744956953E-4
8	1.120036988101495E-5	2.5600845442319887E-5	1.7600581241594922E-5	4.960163804449478E-5	1.9840655217797913E-4	1.136037516502945E-4
9	1.600052840144993E-5	3.200105680289986E-5	2.0800686921884907E-5	5.7601902245219745E-5	2.048067635385591E-4	1.2000396301087448E-4
10-11	2.1600713341957406E-5	3.360110964304485E-5	2.4000792602174893E-5	7.040232496637969E-5	2.488082166425464E-4	1.2480412153130944E-4
12-13	2.8800951122609873E-5	3.760124174340734E-5	2.8800951122609873E-5	7.920261558717716E-5	2.680088507242863E-4	1.376045442524694E-4
14-15	3.6801215323334835E-5	4.080134742369732E-5	3.440113606311735E-5	9.520314398862708E-5	2.9680980184689616E-4	1.536050726539193E-4
16-17	4.080134742369732E-5	4.8001585204349785E-5	3.6801215323334835E-5	1.0480346102949704E-4	3.224106472892161E-4	1.7120565389551424E-4
18-19	4.240140026384231E-5	5.1201690884639774E-5	4.8001585204349785E-5	1.1840391017072947E-4	3.6001188903262343E-4	1.8800620871703666E-4
20-21	4.6401532364204794E-5	5.9201955085364736E-5	5.440179656492976E-5	1.2720420079152694E-4	4.0081323645632075E-4	2.0240668427834162E-4
22-23	4.8001585204349785E-5	7.040232496637969E-5	6.0802007925509734E-5	1.344044385721794E-4	4.344143460993656E-4	2.1760718625971905E-4
24-25	5.200171730471227E-5	7.360243064666967E-5	6.480214002587223E-5	1.4240470277290438E-4	4.8721608982415035E-4	2.2960758256080648E-4
26-27	6.080200792550973E-5	7.440245706674217E-5	6.800224570616221E-5	1.536050726539193E-4	5.376177542887176E-4	2.4560811096225644E-4
28-29	6.240206076565472E-5	8.320274768753963E-5	7.280240422659718E-5	1.6960560105536924E-4	6.064200264149523E-4	2.776091677651563E-4
30-31	6.400211360579972E-5	8.720287978790213E-5	8.160269484739464E-5	1.8800620871703666E-4	6.944229326229269E-4	3.136103566684186E-4
32-33	6.88022721262347E-5	9.040298546819211E-5	8.640285336782962E-5	2.024066842783416E-4	7.640252311692341E-4	3.4721146631146345E-4
34-35	6.960229854630718E-5	1.0080332892913456E-4	9.760322324884458E-5	2.1760718625971905E-4	8.048265785929314E-4	3.8081257595450833E-4
36-37	7.280240422659717E-5	1.0480346102949704E-4	1.0560348744956954E-4	2.3040760898087898E-4	8.480280052768463E-4	4.176137912778431E-4
38-39	7.840258916710466E-5	1.1440377807036699E-4	1.1600383091051199E-4	2.3760784676153147E-4	8.960295904811961E-4	4.568150858613955E-4
40-41	8.240272126746714E-5	1.2160401585101947E-4	1.2000396301087448E-4	2.4240800528196643E-4	9.672319418676481E-4	4.960163804449478E-4
42-43	8.400277410761212E-5	1.336044121521069E-4	1.2480412153130944E-4	2.520083223228364E-4	0.0010336341347336655	5.400178335489351E-4
44-45	9.200303830833709E-5	1.4080464993275937E-4	1.2960428005174442E-4	2.6480874504399634E-4	0.0010952361690792476	5.8641936591314E-4
46-47	9.600317040869957E-5	1.4400475561304938E-4	1.336044121521069E-4	2.808092734454463E-4	0.001171238678986135	6.296207925970547E-4
48-49	1.0640351386964203E-4	1.4800488771341184E-4	1.3920459709261437E-4	2.944097225866787E-4	0.0012888425627367917	6.832225627419119E-4
50-51	1.1200369881014951E-4	1.5280504623384683E-4	1.4720486129333934E-4	3.032100132074762E-4	0.0013888458652458538	7.264239894258268E-4
52-53	1.136037516502945E-4	1.6160533685464427E-4	1.6160533685464427E-4	3.1121027740820114E-4	0.0015080498018366557	7.800257595706841E-4
54-55	1.1920393659080197E-4	1.7200568031558672E-4	1.7280570673565925E-4	3.200105680289986E-4	0.0016176534213865879	8.544282166374263E-4
56-57	1.2640417437145444E-4	1.784058916761667E-4	1.776058652560942E-4	3.2801083222972356E-4	0.0017216568559960126	9.408310700052559E-4
58-59	1.336044121521069E-4	1.8640615587689168E-4	1.8400607661667417E-4	3.38411175690666E-4	0.001844860924687177	0.0010360342139938829
60-61	1.4400475561304938E-4	1.8960626155718166E-4	1.9200634081739914E-4	3.488115191516085E-4	0.001987265627460081	0.00113123735798251
62-63	1.4880491413348434E-4	1.9600647291776163E-4	1.9920657859805164E-4	3.6161194187276844E-4	0.0021256701981326232	0.0012320406869116445
64-65	1.568051783342093E-4	2.0640681637870407E-4	2.056067899586316E-4	3.7281231175378336E-4	0.002244074108303353	0.0013432443593017216
66-67	1.6400541611486178E-4	2.2400739762029902E-4	2.1040694847906656E-4	3.8641276089501576E-4	0.002420079920719302	0.0014752487186136835
68-69	1.7280570673565925E-4	2.3040760898087898E-4	2.1760718625971905E-4	3.9201294583552325E-4	0.002616886420057136	0.0016160533685464428
70-71	1.7520578599587673E-4	2.3760784676153145E-4	2.2320737120022651E-4	4.112135799172632E-4	0.0028040926023541	0.0017584580713193472
72-73	1.824060237765292E-4	2.464081373823289E-4	2.3040760898087898E-4	4.2481402905849566E-4	0.0030192997093536015	0.0019056629326126865
74-75	1.824060237765292E-4	2.5440840158305383E-4	2.3200766182102399E-4	4.344143460993656E-4	0.0032385069484534654	0.002084068824288853
76-77	1.8560612945681918E-4	2.6240866578377886E-4	2.4000792602174895E-4	4.4801479524059803E-4	0.0034545140818730397	0.0022424740554632078
78-79	1.8960626155718166E-4	2.680088507242863E-4	2.488082166425464E-4	4.584151387015405E-4	0.003696122060734934	0.0024032793658977794
80-81	1.9840655217797913E-4	2.7600911492501126E-4	2.544084015830539E-4	4.6641540290226545E-4	0.003960130779358858	0.0026016859180757585
82-83	2.0800686921884908E-4	2.8560943196588123E-4	2.5680848084327137E-4	4.752156935230629E-4	0.004256140554785681	0.0027912921796329404
84-85	2.1200700131921156E-4	2.944097225866787E-4	2.616086393637064E-4	4.808158784635704E-4	0.004556150462312867	0.0029864986261306293
86-87	2.1600713341957405E-4	3.0000990752718617E-4	2.688088771443588E-4	4.864160634040778E-4	0.004863360607620706	0.0031945054953494787
88-89	2.2560745046044402E-4	3.1121027740820114E-4	2.8160929986551875E-4	5.000165125453103E-4	0.005175370911448979	0.0034073125230887624
>>END_MODULE
