##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674156
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	65201014
Total Bases	6.5 Gbp
Sequences flagged as poor quality	0
Sequence length	61-100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.24250866711981	37.0	36.0	37.0	31.0	37.0
2	34.93160722929861	37.0	35.0	37.0	30.0	37.0
3	34.87324243147507	37.0	35.0	37.0	29.0	37.0
4	34.97854039509263	37.0	35.0	37.0	30.0	37.0
5	34.981649549192596	37.0	35.0	37.0	30.0	37.0
6	34.8187160708881	37.0	35.0	37.0	29.0	37.0
7	34.9635524380035	37.0	35.0	37.0	30.0	37.0
8	34.92708335793673	37.0	35.0	37.0	29.0	37.0
9	34.90253412316563	37.0	35.0	37.0	29.0	37.0
10-11	34.88375676948828	37.0	35.0	37.0	29.0	37.0
12-13	34.788808307490434	37.0	35.0	37.0	29.0	37.0
14-15	34.81809028307443	37.0	35.0	37.0	29.0	37.0
16-17	34.75208392464572	37.0	35.0	37.0	29.0	37.0
18-19	34.70115072903621	37.0	35.0	37.0	29.0	37.0
20-21	34.68954963645197	37.0	35.0	37.0	29.0	37.0
22-23	34.578876518699545	37.0	35.0	37.0	28.0	37.0
24-25	34.552457320679096	37.0	35.0	37.0	28.0	37.0
26-27	34.52436311036513	37.0	35.0	37.0	28.0	37.0
28-29	34.48488284246622	37.0	35.0	37.0	28.0	37.0
30-31	34.394473619689414	37.0	35.0	37.0	28.0	37.0
32-33	34.3468654950673	37.0	35.0	37.0	27.5	37.0
34-35	34.31240231938724	37.0	35.0	37.0	27.0	37.0
36-37	34.24173326506855	37.0	34.0	37.0	27.0	37.0
38-39	34.22217764895497	37.0	34.0	37.0	27.0	37.0
40-41	34.143307587210224	37.0	34.0	37.0	27.0	37.0
42-43	34.08203702169418	37.0	34.0	37.0	26.5	37.0
44-45	34.002623931584864	37.0	34.0	37.0	26.0	37.0
46-47	33.91727470680134	37.0	34.0	37.0	26.0	37.0
48-49	33.95000886029165	37.0	34.0	37.0	26.0	37.0
50-51	33.86403633998698	37.0	34.0	37.0	26.0	37.0
52-53	33.7595025746072	37.0	34.0	37.0	25.0	37.0
54-55	33.65888128672354	37.0	33.5	37.0	25.0	37.0
56-57	33.61470031739077	37.0	33.5	37.0	24.5	37.0
58-59	33.51506327800362	37.0	33.0	37.0	24.5	37.0
60-61	33.411351255978936	37.0	33.0	37.0	24.0	37.0
62-63	33.34399232416834	37.0	33.0	37.0	23.5	37.0
64-65	33.30929113018536	37.0	33.0	37.0	23.5	37.0
66-67	33.21818828754628	37.0	33.0	37.0	23.0	37.0
68-69	33.17862381836569	37.0	33.0	37.0	23.0	37.0
70-71	33.04368352057137	36.5	32.5	37.0	22.5	37.0
72-73	32.918211966494255	36.0	32.0	37.0	22.0	37.0
74-75	32.80960914046679	36.0	32.0	37.0	21.5	37.0
76-77	32.81726078411185	36.0	32.0	37.0	21.5	37.0
78-79	32.73725371366797	36.0	32.0	37.0	21.0	37.0
80-81	32.63057148550298	36.0	32.0	37.0	21.0	37.0
82-83	32.52157134565965	36.0	31.5	37.0	20.0	37.0
84-85	32.40541714611962	36.0	31.0	37.0	20.0	37.0
86-87	32.27942356747004	36.0	31.0	37.0	19.5	37.0
88-89	32.16214679798183	36.0	31.0	37.0	19.0	37.0
90-91	32.04933455695425	36.0	30.5	37.0	18.5	37.0
92-93	31.998660566745983	36.0	30.0	37.0	18.5	37.0
94-95	31.89720342806559	36.0	30.0	37.0	18.0	37.0
96-97	31.862483672142726	36.0	30.0	37.0	18.0	37.0
98-99	32.18831395290623	36.0	30.5	37.0	19.5	37.0
100	34.123345421391164	36.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	215.0
20	13518.0
21	134978.0
22	439660.0
23	750980.0
24	939436.0
25	1091319.0
26	1263936.0
27	1482566.0
28	1751125.0
29	2092130.0
30	2526251.0
31	3100581.0
32	3907297.0
33	5154153.0
34	7411937.0
35	1.2638794E7
36	1.9827308E7
37	674830.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	27.75874518261417	23.680977305019315	24.141387484475665	24.418890027890846
2	30.2995358992979	30.854938194958887	20.25107470111686	18.594451204626353
3	16.231452740164972	25.710539905852414	31.135674444081246	26.92233290990137
4	19.709244809826217	29.060295686199016	28.252285157055667	22.978174346919094
5	22.536129868549512	25.435214827792002	28.643010637413624	23.38564466624486
6	22.142033983426185	26.675091868859575	27.894555445589543	23.288318702124702
7	22.83400209194934	27.018752443566672	27.525855732099863	22.621389732384124
8	21.854598529808626	25.619013978464405	28.12777084463659	24.39861664709038
9	22.355640319059017	25.22583265796017	28.465665678489493	23.952861344491325
10-11	23.505881529017667	25.746306068556045	26.743232533804683	24.004579868621605
12-13	23.39787261631125	26.22774610195614	26.568789777267643	23.805591504464967
14-15	23.636869007252496	26.25820292077492	26.25908142939352	23.845846642579062
16-17	23.72379657953262	26.181744845944742	26.26620719525069	23.828251379271943
18-19	23.51988154873422	26.265997742206142	26.382981227892127	23.83113948116751
20-21	23.746707327844096	26.09528644466213	26.250815770510254	23.90719045698352
22-23	23.7829108016563	26.18890825882089	26.200963052609655	23.827217886913154
24-25	23.607884316218524	26.26768492324715	26.360521146061362	23.763909614472965
26-27	23.844072062566397	26.095015266771938	26.212201958620838	23.848710712040823
28-29	23.903182404430478	26.032402381435134	26.160232077726793	23.904183136407596
30-31	23.727763126961133	26.12041996896974	26.28757845470499	23.864238449364134
32-33	23.90961885817763	26.019908406728515	26.126592169427308	23.943880565666543
34-35	23.938058347463592	26.035264551327238	26.117664843211752	23.909012257997418
36-37	23.79561567134057	26.107256056100255	26.258420072486537	23.838708200072638
38-39	23.989190781840914	25.9634698650966	26.09551703874792	23.951822314314562
40-41	24.050998703617186	25.916516733636996	26.025881364282434	24.006603198463385
42-43	23.85878800782821	26.019094840823403	26.1712450371883	23.950872114160084
44-45	24.037669365775994	25.916202988911714	26.035395089165387	24.01073255614691
46-47	24.07980534679321	25.891298601928092	26.04029429800192	23.988601753276775
48-49	23.937701067336796	25.97954392500957	26.149583166621994	23.93317184103164
50-51	24.158705091122037	25.831108625724326	25.980496962833943	24.02968932031969
52-53	24.184343143733432	25.799173029397764	25.94507798098628	24.071405845882524
54-55	24.015876721816408	25.910158252273234	26.071367009074354	24.002598016836
56-57	24.21116436531538	25.783014082694756	25.952357584359348	24.053463967630513
58-59	24.256708609384727	25.768278070575022	25.92659328197352	24.048420038066727
60-61	24.128890443756642	25.83380493110751	26.03260383336801	24.00470079176783
62-63	24.311709623417997	25.690884758535947	25.89381370025996	24.103591917786098
64-65	24.34342292370067	25.701266158710094	25.855882790580665	24.099428127008572
66-67	24.186169334240546	25.795352001011313	25.99853559507228	24.019943069675865
68-69	24.361066692073397	25.69705373188038	25.852070418967205	24.089809157079017
70-71	24.432797820497925	25.64189335418175	25.81796008094151	24.107348744378815
72-73	24.307475625228854	25.727756367491427	25.91286488141541	24.05190312586431
74-75	24.492284232683673	25.59630897080949	25.75340496263655	24.15800183387029
76-77	24.512948112680743	25.608821570821632	25.747608987744165	24.130621328753463
78-79	24.350995479290077	25.6951460800869	25.904571167088275	24.049287273534745
80-81	24.557586364679594	25.571956426322593	25.730102283373725	24.140354925624088
82-83	24.61952383076878	25.552247128643558	25.685358633504535	24.142870407083127
84-85	24.477867037229135	25.611124600659174	25.80536181867652	24.10564654343517
86-87	24.688503796920532	25.475847394633185	25.643024377376015	24.19262443107027
88-89	24.744355942348115	25.47418995842849	25.58815153156127	24.193302567662123
90-91	24.608291056270943	25.55667642027159	25.718109789238593	24.116922734218875
92-93	24.790640354678136	25.454750550640075	25.567050743533027	24.187558351148763
94-95	24.84985471788388	25.43248692574619	25.512670700697644	24.204987655672287
96-97	24.702445474526503	25.525291897746204	25.63159342811996	24.14066919960734
98-99	24.78574519967774	25.39816511382244	25.546072158593713	24.270017527906106
100	24.90760080413911	24.837224605352603	25.512083091199017	24.743091499309266
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	5.0
2	2.5
3	1.5
4	2.0
5	3.0
6	4.0
7	4.5
8	5.5
9	14.5
10	38.5
11	80.5
12	170.0
13	395.0
14	873.0
15	1847.5
16	3933.5
17	7352.0
18	12121.0
19	18912.5
20	29059.0
21	43957.5
22	65282.5
23	92406.0
24	124428.0
25	162909.0
26	208164.5
27	259635.0
28	317572.5
29	382970.5
30	455454.5
31	537709.0
32	630577.5
33	734561.0
34	854030.5
35	989491.5
36	1143399.5
37	1316037.0
38	1500992.5
39	1698174.0
40	1905968.0
41	2113419.0
42	2309875.5
43	2484576.0
44	2628308.0
45	2734750.0
46	2798159.0
47	2810412.5
48	2776272.0
49	2702268.5
50	2594996.5
51	2462403.5
52	2309702.5
53	2147864.5
54	1983390.0
55	1822064.5
56	1669401.5
57	1532637.0
58	1407052.0
59	1291390.5
60	1190243.5
61	1096085.5
62	1004341.5
63	915238.5
64	827597.0
65	739653.0
66	650298.5
67	562997.0
68	480557.5
69	401290.5
70	327289.5
71	261506.5
72	204299.5
73	155993.5
74	117000.0
75	85781.0
76	61467.0
77	43188.0
78	29897.0
79	20610.0
80	13918.0
81	9181.5
82	5983.5
83	3877.0
84	2572.5
85	1663.5
86	1009.0
87	652.0
88	399.0
89	236.0
90	147.0
91	81.0
92	50.0
93	33.0
94	21.0
95	15.5
96	9.5
97	8.0
98	6.0
99	3.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07432093003952363
2	0.07150195547572312
3	0.1166086159334884
4	0.058094188535166034
5	0.027128412450763417
6	0.28368883956313934
7	0.05368014675354588
8	0.020413792951134165
9	0.05870000733424176
10-11	0.027486535715533503
12-13	0.08721490128972534
14-15	0.051768366669880314
16-17	0.0405085724587044
18-19	0.06347984097302536
20-21	0.028792497000123343
22-23	0.08712364504024432
24-25	0.07625724961884794
26-27	0.031294758698077914
28-29	0.07469822478527711
30-31	0.04865415129893532
32-33	0.017819508144459224
34-35	0.022942127863226178
36-37	0.01874127295014154
38-39	0.08257770346945831
40-41	0.04255992092392919
42-43	0.017107095911115738
44-45	0.12283167866070303
46-47	0.07175195158774678
48-49	0.0034362962514662733
50-51	0.019722852776492095
52-53	0.005789787256989592
54-55	0.04447170100759476
56-57	0.018729770061551496
58-59	0.0495268371133001
60-61	0.06472828781466497
62-63	0.07763913115889778
64-65	0.017964444816217808
66-67	0.05831428160874014
68-69	0.01675817727042704
70-71	0.02287158382892138
72-73	0.017872429929469007
74-75	0.10799072219345687
76-77	0.024565588841550974
78-79	0.02902335345842585
80-81	0.02525118150384083
82-83	0.030935173832517735
84-85	0.03485848831030533
86-87	0.036868596432771385
88-89	0.03963830005190065
90-91	0.048750129789829476
92-93	0.050219194680999384
94-95	0.05757448894274686
96-97	0.038383867136952604
98-99	0.05141051083461123
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
61	1.0
62	0.0
63	0.0
64	0.0
65	4.0
66	0.0
67	2.0
68	4.0
69	8.0
70	2.0
71	7.0
72	6.0
73	6.0
74	5.0
75	5.0
76	6.0
77	10.0
78	11.0
79	17.0
80	21.0
81	22.0
82	30.0
83	47.0
84	76.0
85	123.0
86	252.0
87	435.0
88	694.0
89	1290.0
90	2361.0
91	4628.0
92	9421.0
93	19261.0
94	41121.0
95	92199.0
96	216870.0
97	538402.0
98	1542177.0
99	6053237.0
100	5.6678253E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.01380723075518
#Duplication Level	Percentage of total
1	58.315438524391425
2	21.119287854020282
3	9.003184810574709
4	4.275217020163098
5	2.3031144251070774
6	1.3941634287268576
7	0.964618491637278
8	0.6228121964133322
9	0.4091598085075294
>10	1.5590816343654514
>50	0.02198334525658852
>100	0.0067258410438150885
>500	0.0
>1k	0.005212619792491335
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	9.202310872036437E-6	9.202310872036437E-6	1.5337184786727398E-6	6.134873914690959E-6	1.0736029350709177E-5	5.214642827487315E-5
2	1.6870903265400135E-5	1.0736029350709177E-5	7.668592393363698E-6	1.2269747829381918E-5	4.447783588150945E-5	6.441617610425507E-5
3	2.1472058701418354E-5	1.2269747829381918E-5	1.3803466308054658E-5	1.6870903265400135E-5	5.6747583710891366E-5	7.821964241230973E-5
4	2.7606932616109315E-5	1.3803466308054658E-5	1.9938340222745614E-5	2.1472058701418354E-5	7.515220545496424E-5	9.509054567770986E-5
5	3.2208088052127534E-5	1.8404621744072875E-5	1.9938340222745614E-5	2.6073214137436576E-5	8.89556717630189E-5	1.1349516742178273E-4
6	3.680924348814575E-5	1.8404621744072875E-5	2.1472058701418354E-5	2.9140651094782055E-5	9.969170111372807E-5	1.1656260437912822E-4
7	3.680924348814575E-5	2.3005777180091097E-5	2.4539495658763836E-5	3.067436957345479E-5	1.0889401198576452E-4	1.2269747829381916E-4
8	3.834296196681849E-5	2.9140651094782055E-5	2.4539495658763836E-5	3.374180653080027E-5	1.1349516742178273E-4	1.2729863372983738E-4
9	3.834296196681849E-5	3.2208088052127534E-5	2.6073214137436576E-5	3.374180653080027E-5	1.1656260437912822E-4	1.3343350764452835E-4
10-11	4.447783588150945E-5	3.834296196681849E-5	3.374180653080028E-5	4.217725816350034E-5	1.2806549296917377E-4	1.480038331919194E-4
12-13	5.828130218956411E-5	4.1410398924163964E-5	3.987668044549123E-5	5.061270979620041E-5	1.4953755167059212E-4	1.6487473645731952E-4
14-15	7.515220545496424E-5	4.447783588150945E-5	4.447783588150945E-5	5.751444295022773E-5	1.771444842867014E-4	1.8404621744072877E-4
16-17	8.818881252368254E-5	5.137956903553678E-5	4.83121320781913E-5	6.59498945829278E-5	1.855799359194015E-4	2.0168397994546524E-4
18-19	1.0352599731040992E-4	5.9048161428900474E-5	5.368014675354589E-5	6.978419077960965E-5	2.1395372777484718E-4	2.2622347560422907E-4
20-21	1.0889401198576452E-4	6.671675382226418E-5	5.9815020668236846E-5	7.438534621562786E-5	2.453949565876383E-4	2.5306354898100203E-4
22-23	1.1349516742178273E-4	7.438534621562786E-5	6.441617610425507E-5	7.898650165164609E-5	2.722350299644113E-4	2.768361854004295E-4
24-25	1.295992114478465E-4	7.975336089098247E-5	6.978419077960965E-5	8.742195328434617E-5	2.975413848625115E-4	3.0520997725587515E-4
26-27	1.5107127014926485E-4	9.125624948102801E-5	8.052022013031883E-5	9.66242641563826E-5	3.320500506326481E-4	3.4662037618003917E-4
28-29	1.6487473645731952E-4	1.0505971578908267E-4	8.742195328434616E-5	9.969170111372809E-5	3.788284642321667E-4	3.8803077510420313E-4
30-31	1.9018109135541974E-4	1.1502888590045548E-4	9.66242641563826E-5	1.042928565497463E-4	4.325086109857126E-4	4.2944117402836715E-4
32-33	2.1011943157816533E-4	1.2039690057581006E-4	1.065934342677554E-4	1.1042773046443725E-4	4.631829805591674E-4	4.7085157295253106E-4
34-35	2.162543054928563E-4	1.272986337298374E-4	1.1272830818244637E-4	1.1809632285780095E-4	4.984585055686404E-4	5.107282533980223E-4
36-37	2.292909125615746E-4	1.3650094460187384E-4	1.1579574513979185E-4	1.2883235220851014E-4	5.398689044928044E-4	5.590403854762136E-4
38-39	2.5229668974166564E-4	1.449363962345739E-4	1.2346433753315556E-4	1.3573408536253744E-4	5.858804588529865E-4	6.01217643639714E-4
40-41	2.637995783317112E-4	1.5107127014926485E-4	1.3113292992651926E-4	1.4340267775590115E-4	6.36493168649187E-4	6.518303534359144E-4
42-43	2.768361854004295E-4	1.6027358102130128E-4	1.349672261232011E-4	1.5413870710661036E-4	6.940076115994147E-4	7.116453741041512E-4
44-45	2.868053555118023E-4	1.625741587393104E-4	1.403352407985557E-4	1.6487473645731952E-4	7.400191659595969E-4	7.852638610804426E-4
46-47	2.9370708866582965E-4	1.6640845493599225E-4	1.4953755167059212E-4	1.7177646961134683E-4	8.029016235851792E-4	8.650172219714252E-4
48-49	3.113448511705662E-4	1.7254332885068322E-4	1.556724255852831E-4	1.8174563972271965E-4	8.696183774074434E-4	9.409362866657257E-4
50-51	3.251483174786208E-4	1.7637762504736507E-4	1.6487473645731952E-4	1.8788051363741062E-4	9.30967116554353E-4	0.001049063439412154
52-53	3.335837691113209E-4	1.8634679515873787E-4	1.6794217341466498E-4	1.8941423211608335E-4	9.861809817865717E-4	0.0011625586068339366
54-55	3.427860799833573E-4	1.9554910603077432E-4	1.7177646961134683E-4	1.932485283127652E-4	0.0010482965801728176	0.0012798880704524012
56-57	3.5045467237672097E-4	1.9784968374878342E-4	1.8021192124404692E-4	1.9861654298811978E-4	0.0011265162225851271	0.0014209901704902933
58-59	3.703930125994666E-4	2.0398455766347436E-4	1.8404621744072877E-4	2.0398455766347436E-4	0.0011993678503220825	0.0015659265667248672
60-61	3.8266276042884855E-4	2.0705199462081988E-4	1.9018109135541972E-4	2.085857130994926E-4	0.001283722366649083	0.0017354024586182049
62-63	3.9799994521557596E-4	2.1472058701418355E-4	1.9478224679143793E-4	2.1778802397152904E-4	0.0013926163786348476	0.00190334463203287
64-65	4.0566853760893966E-4	2.2468975712555634E-4	1.9708282450944705E-4	2.2162232016821089E-4	0.0015022772498599485	0.0020866239902342623
66-67	4.2177258163500345E-4	2.292909125615746E-4	2.001502614667925E-4	2.2699033484356547E-4	0.0016242078689144312	0.002274504503871673
68-69	4.363429071823944E-4	2.3312520875825644E-4	2.0475141690281075E-4	2.315914902795837E-4	0.0017568745173196232	0.0024884582316465204
70-71	4.509132327297855E-4	2.3772636419427463E-4	2.131868685355108E-4	2.3465892723692917E-4	0.00191178008366557	0.002745356076824204
72-73	4.647166990378401E-4	2.415606603909565E-4	2.1702116473219264E-4	2.3772636419427463E-4	0.0020697530869688624	0.0029968859073265337
74-75	4.7621958762788564E-4	2.4846239354498384E-4	2.185548832108654E-4	2.4309437886962923E-4	0.0022208243571181273	0.0032491825970681993
76-77	4.930904908932857E-4	2.5536412669901115E-4	2.2468975712555634E-4	2.469286750663111E-4	0.002415606603909565	0.00352141762703261
78-79	5.045933794833313E-4	2.6533329681038397E-4	2.3005777180091095E-4	2.4999611202365657E-4	0.00256667787405883	0.003828161322767158
80-81	5.168631273127133E-4	2.722350299644113E-4	2.3465892723692917E-4	2.538304082203384E-4	0.002723117158883449	0.004118034115236306
82-83	5.24531719706077E-4	2.79903622357775E-4	2.461618158269747E-4	2.59198422895693E-4	0.002893359910016123	0.0044370475588002365
84-85	5.283660159027589E-4	2.8910593322981147E-4	2.5766470441702027E-4	2.6533329681038397E-4	0.0031073136377909706	0.004835047504015812
86-87	5.437032006894863E-4	2.9370708866582965E-4	2.6073214137436573E-4	2.699344522464022E-4	0.0033412057057885637	0.0052330474492313876
88-89	5.498380746041772E-4	3.0597683649521154E-4	2.6533329681038397E-4	2.7836990387910225E-4	0.0035819995069401836	0.0056509857346697095
>>END_MODULE
