##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674159
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29358125
Total Bases	2.9 Gbp
Sequences flagged as poor quality	0
Sequence length	62-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.14640839631277	37.0	35.0	37.0	30.0	37.0
2	34.73528353735124	37.0	35.0	37.0	29.0	37.0
3	34.9124552402444	37.0	35.0	37.0	30.0	37.0
4	34.91328673067507	37.0	35.0	37.0	29.0	37.0
5	34.91282832265344	37.0	35.0	37.0	29.0	37.0
6	34.73562340919251	37.0	35.0	37.0	29.0	37.0
7	34.94685757349967	37.0	35.0	37.0	30.0	37.0
8	34.9225142613842	37.0	35.0	37.0	29.0	37.0
9	34.919260511357585	37.0	35.0	37.0	29.0	37.0
10-11	34.90981510229281	37.0	35.0	37.0	29.0	37.0
12-13	34.83576110531582	37.0	35.0	37.0	29.0	37.0
14-15	34.916070184999896	37.0	35.0	37.0	29.0	37.0
16-17	34.83738753752156	37.0	35.0	37.0	29.0	37.0
18-19	34.818956489898454	37.0	35.0	37.0	29.0	37.0
20-21	34.8101158708194	37.0	35.0	37.0	29.0	37.0
22-23	34.726071368658594	37.0	35.0	37.0	29.0	37.0
24-25	34.73814126413046	37.0	35.0	37.0	29.0	37.0
26-27	34.69883689779235	37.0	35.0	37.0	29.0	37.0
28-29	34.68633310199476	37.0	35.0	37.0	29.0	37.0
30-31	34.61055798011624	37.0	35.0	37.0	28.0	37.0
32-33	34.53943877546676	37.0	35.0	37.0	28.0	37.0
34-35	34.53068590041087	37.0	35.0	37.0	28.0	37.0
36-37	34.49843343197156	37.0	35.0	37.0	28.0	37.0
38-39	34.486403678709046	37.0	35.0	37.0	28.0	37.0
40-41	34.39240918825708	37.0	35.0	37.0	28.0	37.0
42-43	34.37321388882975	37.0	35.0	37.0	27.5	37.0
44-45	34.25523782598514	37.0	34.5	37.0	27.0	37.0
46-47	34.26975615779277	37.0	34.5	37.0	27.0	37.0
48-49	34.31436041981564	37.0	34.5	37.0	27.0	37.0
50-51	34.22638199816916	37.0	34.0	37.0	27.0	37.0
52-53	34.17447023609307	37.0	34.0	37.0	27.0	37.0
54-55	34.08180989078832	37.0	34.0	37.0	26.5	37.0
56-57	34.05662773082409	37.0	34.0	37.0	26.5	37.0
58-59	33.98211644987546	37.0	34.0	37.0	26.0	37.0
60-61	33.889751610499644	37.0	34.0	37.0	26.0	37.0
62-63	33.900788553138455	37.0	34.0	37.0	26.0	37.0
64-65	33.82389465689293	37.0	34.0	37.0	25.5	37.0
66-67	33.81142408523797	37.0	34.0	37.0	25.5	37.0
68-69	33.77981115916066	37.0	34.0	37.0	25.0	37.0
70-71	33.66267075898153	37.0	33.5	37.0	25.0	37.0
72-73	33.548671569978914	37.0	33.0	37.0	24.5	37.0
74-75	33.54049843258687	37.0	33.0	37.0	24.5	37.0
76-77	33.50898622391792	37.0	33.0	37.0	24.0	37.0
78-79	33.46181049833695	37.0	33.0	37.0	24.0	37.0
80-81	33.40654008259395	37.0	33.0	37.0	24.0	37.0
82-83	33.31124259135696	37.0	33.0	37.0	23.5	37.0
84-85	33.20916356339177	37.0	33.0	37.0	23.0	37.0
86-87	33.100258565049614	36.5	33.0	37.0	22.5	37.0
88-89	33.05262585761133	36.0	32.5	37.0	23.0	37.0
90-91	32.95483558826655	36.0	32.0	37.0	22.0	37.0
92-93	32.85440588748648	36.0	32.0	37.0	22.0	37.0
94-95	32.82624386636239	36.0	32.0	37.0	22.0	37.0
96-97	32.786201661360565	36.0	32.0	37.0	21.5	37.0
98-99	32.928023555139944	36.0	32.0	37.0	22.0	37.0
100	34.41702927969318	36.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	54.0
20	4197.0
21	44447.0
22	146002.0
23	244834.0
24	308372.0
25	366355.0
26	436769.0
27	527818.0
28	645181.0
29	793677.0
30	986691.0
31	1239598.0
32	1601703.0
33	2168277.0
34	3264544.0
35	6180952.0
36	1.0161513E7
37	237141.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	25.45985150468476	31.125498247411016	21.428451837160576	21.98619841074365
2	25.019629041549322	30.661826319785142	27.086580330597222	17.231964308068314
3	18.7032998596331	28.24826620301678	30.316454838462683	22.73197909888744
4	20.477740206608264	28.928626000657825	28.640875324192756	21.952758468541152
5	21.8889516683137	27.93341065665192	28.38780812588717	21.789829549147207
6	21.73284413745505	27.692931560049626	28.370326574880245	22.203897727615075
7	22.067120321440242	28.368544114229167	27.931296955880814	21.633038608449777
8	20.931262841103006	27.648396245182592	29.100938949698214	22.319401964016187
9	21.42391517464293	27.205492961625648	29.090594067100778	22.279997796630642
10-11	21.894357578988156	27.84134794204943	28.25968188166384	22.00461259729857
12-13	21.881532859781583	27.884687802126646	28.089654678881757	22.144124659210014
14-15	21.85612515306054	27.968546384666652	28.10336881944423	22.071959642828578
16-17	22.05784470819264	27.97603832026045	28.01397783959984	21.952139131947067
18-19	22.012208713483968	27.785986971228375	27.991447819116367	22.210356496171286
20-21	22.01545886700132	27.773097585691076	28.068164376600457	22.143279170707146
22-23	22.17676402468326	27.946894741291047	27.87157440494847	22.004766829077223
24-25	22.16674854760701	27.783015621656908	27.858595931528	22.19163989920808
26-27	22.11877331557788	27.77415994569492	27.960592556252085	22.14647418247512
28-29	22.292065080751126	27.83632260480047	27.808874250069305	22.062738064379104
30-31	22.267028336295517	27.691232952533607	27.77010245659452	22.27163625457636
32-33	22.20266822539907	27.70847343212471	27.872160435237664	22.216697907238558
34-35	22.333837518783458	27.84715617738806	27.715837205629306	22.103169098199174
36-37	22.316013363604945	27.68346360368718	27.712646102883294	22.28787692982458
38-39	22.256683804771757	27.7052732200383	27.81279960354767	22.22524337164227
40-41	22.393427329000833	27.770708122266168	27.696046720440282	22.139817828292717
42-43	22.349038890352166	27.65302956874629	27.68596311636824	22.311968424533305
44-45	22.282603843377363	27.673652314702124	27.77930706387977	22.264436778040746
46-47	22.404595224869844	27.77777323213226	27.686115972394887	22.131515570603007
48-49	22.378097528585297	27.639227616504385	27.655591341647824	22.327083513262494
50-51	22.315057150694173	27.63713525863053	27.766558708729942	22.281248881945356
52-53	22.44207972082397	27.71810729670574	27.659777578606825	22.180035403863467
54-55	22.394911892243655	27.62496613849133	27.629172515911716	22.3509494533533
56-57	22.344902212554732	27.610096209430253	27.76532716611414	22.279674411900878
58-59	22.47020697834361	27.70415472016323	27.626588481525193	22.19904981996796
60-61	22.4545976952915	27.56245562174655	27.616044456097022	22.36690222686492
62-63	22.366414966464838	27.59674430797363	27.725089672192986	22.311751053368543
64-65	22.52106713759911	27.68791992354105	27.59752310747956	22.19348983138028
66-67	22.482651573631408	27.564629717222278	27.598654189289313	22.354064519857
68-69	22.39967884627192	27.600100590470834	27.702235855699854	22.29798470755739
70-71	22.54931712392885	27.679931603799098	27.575255708562548	22.195495563709507
72-73	22.52378731374267	27.54609945216972	27.547966256021482	22.382146978066128
74-75	22.451883580939676	27.569178934568512	27.670677912544267	22.30825957194755
76-77	22.550695294573675	27.675416512651267	27.565624015208428	22.20826417756663
78-79	22.54171377593723	27.542046732839314	27.547598569850873	22.36864092137258
80-81	22.47583840422245	27.58255446088923	27.636210559735545	22.305396575152773
82-83	22.621630775593925	27.640163876796347	27.523237523992737	22.214967823616988
84-85	22.59799182063604	27.521021742938917	27.489142364475715	22.391844071949333
86-87	22.551199523517145	27.522673444414092	27.598270330704093	22.327856701364666
88-89	22.67678760644963	27.610259432335205	27.45934404500933	22.253608916205838
90-91	22.657241488707182	27.483492137648696	27.4500990173573	22.409167356286822
92-93	22.609893047968846	27.502108882684595	27.541054293918638	22.346943775427917
94-95	22.72452703173913	27.586812799321773	27.424349717130426	22.264310451808672
96-97	22.699354369008088	27.461514816432004	27.408678457390838	22.430452357169074
98-99	22.57456822023348	27.47562515530944	27.546827694365376	22.402978930091695
100	22.9942249697726	27.017866745148932	27.248020816927284	22.739887468151185
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	0.5
4	1.0
5	1.5
6	1.5
7	2.0
8	16.5
9	39.0
10	46.5
11	95.0
12	218.0
13	615.0
14	1156.5
15	1498.5
16	2365.5
17	4158.0
18	7075.5
19	11442.0
20	17606.0
21	25873.5
22	37618.0
23	54070.5
24	76540.0
25	106146.0
26	140194.0
27	180302.0
28	226751.5
29	278822.5
30	340881.5
31	410429.5
32	478007.0
33	545292.0
34	620806.5
35	696475.5
36	771243.0
37	848326.5
38	925182.5
39	999596.5
40	1073148.5
41	1153055.0
42	1233189.5
43	1298232.5
44	1342785.5
45	1368094.5
46	1371799.5
47	1349293.0
48	1302589.0
49	1242526.0
50	1168961.5
51	1083333.5
52	988791.5
53	885064.0
54	781368.5
55	680523.5
56	583007.5
57	494592.5
58	415422.5
59	344949.5
60	283706.5
61	232902.5
62	191066.0
63	155160.5
64	124913.0
65	99793.0
66	79606.5
67	63038.0
68	48940.5
69	37743.5
70	28816.5
71	21299.0
72	15520.5
73	11220.0
74	7866.5
75	5435.0
76	3740.5
77	2484.5
78	1618.5
79	1064.5
80	707.5
81	463.0
82	272.5
83	162.5
84	104.5
85	70.0
86	46.0
87	22.5
88	15.5
89	10.5
90	5.5
91	3.5
92	1.5
93	1.0
94	1.0
95	1.0
96	1.5
97	1.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1092780959274477
2	0.11224831286057949
3	0.2186175036723224
4	0.04403891597300577
5	0.052905286015370534
6	0.5050901581759735
7	0.0339394971579418
8	0.04063951631788474
9	0.07233772592766056
10-11	0.03338428458901922
12-13	0.13948097843441978
14-15	0.04885870606518638
16-17	0.03439933578864454
18-19	0.022302173589083093
20-21	0.045687522619377086
22-23	0.04584931769314287
24-25	0.022361782300470485
26-27	0.04863389606795393
28-29	0.05363762161241564
30-31	0.0220586294254146
32-33	0.020674004215187447
34-35	0.017606710237796183
36-37	0.03425286866923552
38-39	0.01973559278734592
40-41	0.06244608604943265
42-43	0.0017797458114235838
44-45	0.23455006067315265
46-47	0.08864326315117195
48-49	0.0017252464181551103
50-51	0.03499882911459775
52-53	0.002987247993528197
54-55	0.07397611393779405
56-57	0.02171971132352628
58-59	0.07242799054776149
60-61	0.0805773529474379
62-63	0.11091307961446924
64-65	0.021890022673110857
66-67	0.024492369207452923
68-69	0.004983289035822304
70-71	0.019113962989250097
72-73	0.01065633848277856
74-75	0.008995811004039804
76-77	0.007735513692267981
78-79	0.025364386759194026
80-81	0.008861276872358595
82-83	0.02997304690915535
84-85	0.03357519543449554
86-87	0.020893884951879344
88-89	0.014882020897368488
90-91	0.03678721743064509
92-93	0.05480473246206402
94-95	0.05545528334316438
96-97	0.019919001639551435
98-99	0.04501101348986715
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62	1.0
63	0.0
64	0.0
65	0.0
66	1.0
67	1.0
68	3.0
69	0.0
70	0.0
71	5.0
72	0.0
73	2.0
74	6.0
75	2.0
76	4.0
77	3.0
78	10.0
79	9.0
80	13.0
81	12.0
82	20.0
83	33.0
84	35.0
85	52.0
86	82.0
87	131.0
88	256.0
89	416.0
90	727.0
91	1524.0
92	2945.0
93	5838.0
94	12277.0
95	27625.0
96	65073.0
97	165066.0
98	474911.0
99	2111830.0
100	2.6489212E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.01394669449012
#Duplication Level	Percentage of total
1	54.72443969760036
2	19.588004415450044
3	8.944891591263957
4	4.4891192666737805
5	2.25642771633823
6	1.3014994582302986
7	1.0218381032450192
8	0.8924845845547326
9	0.7859194309054068
>10	5.675454379779313
>50	0.2840927416817379
>100	0.019553609729542284
>500	0.0022974780685614316
>1k	0.01397752647899832
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	6.812424158559172E-6	3.406212079279586E-6	3.406212079279586E-6	1.0218636237838758E-5	1.3624848317118344E-5	1.3624848317118344E-5
2	1.0218636237838758E-5	3.406212079279586E-6	6.812424158559172E-6	1.3624848317118344E-5	3.4062120792795864E-5	1.7031060396397932E-5
3	1.7031060396397932E-5	3.406212079279586E-6	1.0218636237838758E-5	2.724969663423669E-5	4.087454495135503E-5	2.3843484554957104E-5
4	2.0437272475677516E-5	6.812424158559172E-6	1.0218636237838758E-5	3.4062120792795864E-5	4.768696910991421E-5	3.0655908713516276E-5
5	2.0437272475677516E-5	6.812424158559172E-6	1.0218636237838758E-5	3.7468332872075445E-5	6.131181742703255E-5	3.4062120792795864E-5
6	2.0437272475677516E-5	1.3624848317118344E-5	1.3624848317118344E-5	3.7468332872075445E-5	7.834287782343048E-5	4.087454495135503E-5
7	2.0437272475677516E-5	2.0437272475677516E-5	1.3624848317118344E-5	4.087454495135503E-5	9.537393821982842E-5	4.768696910991421E-5
8	2.0437272475677516E-5	2.0437272475677516E-5	1.3624848317118344E-5	5.109318118919379E-5	1.0899878653694675E-4	6.471802950631213E-5
9	2.3843484554957104E-5	2.3843484554957104E-5	1.7031060396397932E-5	5.449939326847338E-5	1.1581121069550593E-4	7.153045366487131E-5
10-11	2.3843484554957104E-5	3.4062120792795864E-5	1.8734166436037723E-5	6.131181742703255E-5	1.39654695250463E-4	7.32335597045111E-5
12-13	2.3843484554957104E-5	4.087454495135503E-5	2.0437272475677516E-5	7.153045366487131E-5	1.7541992208289868E-4	9.026462010090905E-5
14-15	2.3843484554957104E-5	4.428075703063462E-5	2.3843484554957104E-5	8.515530198198966E-5	2.0607583079641496E-4	1.0218636237838759E-4
16-17	2.5546590594596898E-5	4.76869691099142E-5	2.3843484554957104E-5	8.685840802162945E-5	2.452472697081302E-4	1.1921742277478552E-4
18-19	3.4062120792795864E-5	6.301492346667234E-5	2.3843484554957104E-5	9.367083218018863E-5	2.656845421838077E-4	1.2943605901262427E-4
20-21	4.087454495135503E-5	6.812424158559173E-5	2.724969663423669E-5	1.0388946841802737E-4	2.8101249654056585E-4	1.3284227109190385E-4
22-23	4.598386307027441E-5	7.153045366487131E-5	2.724969663423669E-5	1.0729568049730696E-4	3.0826219317480255E-4	1.447640133693824E-4
24-25	5.620249930811317E-5	7.66397717837907E-5	2.724969663423669E-5	1.2773295297298446E-4	3.610584804036361E-4	1.4987333148830178E-4
26-27	6.131181742703255E-5	8.345219594234986E-5	2.724969663423669E-5	1.4306090732974262E-4	4.1044855555319015E-4	1.6349817980542013E-4
28-29	6.301492346667234E-5	8.685840802162945E-5	2.724969663423669E-5	1.48170225448662E-4	4.5302620654418495E-4	1.7712302812253848E-4
30-31	6.982734762523152E-5	9.196772614054882E-5	3.0655908713516276E-5	1.5668575564686097E-4	5.143380239712175E-4	2.179975730738935E-4
32-33	7.153045366487131E-5	9.537393821982842E-5	3.7468332872075445E-5	1.6009196772614055E-4	5.518063568432929E-4	2.452472697081302E-4
34-35	7.493666574415089E-5	9.537393821982842E-5	4.428075703063462E-5	1.6009196772614055E-4	5.807591595171694E-4	2.6227833010452814E-4
36-37	8.685840802162945E-5	9.537393821982842E-5	4.768696910991421E-5	1.6009196772614055E-4	6.165243863496051E-4	2.827156025802057E-4
38-39	9.026462010090903E-5	1.0218636237838759E-4	4.768696910991421E-5	1.6349817980542013E-4	6.50586507142401E-4	2.96340450897324E-4
40-41	9.196772614054882E-5	1.1240499861622634E-4	5.109318118919379E-5	1.669043918846997E-4	7.101952185297937E-4	3.1507461733336177E-4
42-43	9.196772614054882E-5	1.209205288144253E-4	5.449939326847338E-5	1.754199220828987E-4	7.612883997189874E-4	3.3551188980903926E-4
44-45	9.537393821982842E-5	1.2773295297298446E-4	5.620249930811317E-5	1.7712302812253848E-4	8.174908990271007E-4	3.6446469248291574E-4
46-47	9.8780150299108E-5	1.3284227109190385E-4	5.960871138739276E-5	1.8393545228109764E-4	8.89021352691972E-4	3.900112830775126E-4
48-49	1.0218636237838758E-4	1.3284227109190385E-4	6.812424158559173E-5	1.8904477040001703E-4	9.452238520000852E-4	4.206671917910289E-4
50-51	1.0729568049730696E-4	1.3454537713154365E-4	7.153045366487131E-5	2.0777893683605474E-4	9.860983969514402E-4	4.547293125838248E-4
52-53	1.1751431673514572E-4	1.447640133693824E-4	7.32335597045111E-5	2.179975730738935E-4	0.0010627381687352308	5.075255998126583E-4
54-55	1.2773295297298446E-4	1.48170225448662E-4	7.493666574415089E-5	2.231068911928129E-4	0.0011376748344793818	5.501032508036532E-4
56-57	1.3795158921082323E-4	1.5157643752794158E-4	8.174908990271007E-5	2.3843484554957105E-4	0.0012330487726992102	5.773529474378898E-4
58-59	1.4306090732974262E-4	1.5668575564686097E-4	8.174908990271007E-5	2.452472697081302E-4	0.001290954378046963	6.148212803099653E-4
60-61	1.464671194090222E-4	1.6009196772614055E-4	8.685840802162945E-5	2.5205969386668937E-4	0.001374406573989313	6.710237796180784E-4
62-63	1.48170225448662E-4	1.669043918846997E-4	9.026462010090903E-5	2.7590317842164647E-4	0.0014697805122091414	7.204138547676325E-4
64-65	1.6179507376578033E-4	1.7031060396397932E-4	9.196772614054882E-5	2.8612181465948524E-4	0.0015600451323100504	7.578821876397079E-4
66-67	1.737168160432589E-4	1.737168160432589E-4	9.196772614054882E-5	2.96340450897324E-4	0.0016758563430055566	8.055691567496221E-4
68-69	1.8052924020181806E-4	1.8052924020181806E-4	9.196772614054882E-5	2.9974666297660356E-4	0.0017950737657803418	8.532561258595363E-4
70-71	1.8393545228109764E-4	1.8393545228109764E-4	9.196772614054882E-5	3.065590871351628E-4	0.0019568688395461224	9.247865795244077E-4
72-73	1.8563855832073742E-4	1.8904477040001703E-4	9.53739382198284E-5	3.167777233730015E-4	0.0021203670193515422	9.809890788325208E-4
74-75	1.9074787643965683E-4	1.958571945585762E-4	1.0559257445766717E-4	3.286994656504801E-4	0.0022549123964830864	0.001054222638537032
76-77	1.9245098247929664E-4	2.0096651267749558E-4	1.1410810465586614E-4	3.3721499584867903E-4	0.0023758329252975114	0.001127456198241543
78-79	1.97560300598216E-4	2.1288825495497412E-4	1.1751431673514572E-4	3.576522683243565E-4	0.002517190726587614	0.0012075021821046131
80-81	2.0266961871713535E-4	2.1629446703425373E-4	1.1921742277478551E-4	3.6446469248291574E-4	0.0026636578459966363	0.0012620015753730866
82-83	2.1459136099461393E-4	2.231068911928129E-4	1.209205288144253E-4	3.763864347603943E-4	0.002767547314414664	0.001340344453196517
84-85	2.2140378515317312E-4	2.3332552743065166E-4	1.243267408937049E-4	3.8660507099823305E-4	0.0029736231452110787	0.001447640133693824
86-87	2.2991931535137206E-4	2.3843484554957105E-4	1.2943605901262427E-4	4.002299193153514E-4	0.003160964809571456	0.0015276861175568944
88-89	2.418410576288506E-4	2.452472697081302E-4	1.3284227109190385E-4	4.121516615928299E-4	0.0033397909437336342	0.0016060289953803248
>>END_MODULE
