##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674170
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33303969
Total Bases	3.3 Gbp
Sequences flagged as poor quality	0
Sequence length	66-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.60997786780308	37.0	36.0	37.0	32.0	37.0
2	35.217473959334995	37.0	36.0	37.0	31.0	37.0
3	35.42890686692628	37.0	36.0	37.0	31.0	37.0
4	35.50166098821435	37.0	36.0	37.0	32.0	37.0
5	35.46114836943308	37.0	36.0	37.0	31.0	37.0
6	35.366205000971505	37.0	36.0	37.0	31.0	37.0
7	35.43952352946281	37.0	36.0	37.0	31.0	37.0
8	35.41255416133735	37.0	36.0	37.0	31.0	37.0
9	35.423858189394785	37.0	36.0	37.0	31.0	37.0
10-11	35.34469885556283	37.0	36.0	37.0	31.0	37.0
12-13	35.38803305996352	37.0	36.0	37.0	31.0	37.0
14-15	35.41494371136365	37.0	36.0	37.0	31.0	37.0
16-17	35.34765389374462	37.0	36.0	37.0	31.0	37.0
18-19	35.30254158295668	37.0	36.0	37.0	31.0	37.0
20-21	35.253670651086665	37.0	36.0	37.0	31.0	37.0
22-23	35.263473776954335	37.0	36.0	37.0	31.0	37.0
24-25	35.285667663214554	37.0	36.0	37.0	31.0	37.0
26-27	35.200473838418475	37.0	36.0	37.0	31.0	37.0
28-29	35.25816735236572	37.0	36.0	37.0	31.0	37.0
30-31	35.16503187653099	37.0	36.0	37.0	31.0	37.0
32-33	35.11142538296261	37.0	36.0	37.0	30.0	37.0
34-35	35.08349557375579	37.0	36.0	37.0	30.0	37.0
36-37	35.061891482063295	37.0	36.0	37.0	30.0	37.0
38-39	35.034825548870764	37.0	35.0	37.0	30.0	37.0
40-41	34.97294244418735	37.0	35.0	37.0	30.0	37.0
42-43	34.95479574821847	37.0	35.0	37.0	30.0	37.0
44-45	34.90738129440368	37.0	35.0	37.0	30.0	37.0
46-47	34.88651131941661	37.0	35.0	37.0	29.5	37.0
48-49	34.89113997193547	37.0	35.0	37.0	29.5	37.0
50-51	34.84989587877649	37.0	35.0	37.0	29.0	37.0
52-53	34.75013215391835	37.0	35.0	37.0	29.0	37.0
54-55	34.69910188482339	37.0	35.0	37.0	29.0	37.0
56-57	34.68584751865461	37.0	35.0	37.0	29.0	37.0
58-59	34.632379597158526	37.0	35.0	37.0	29.0	37.0
60-61	34.56377269928398	37.0	35.0	37.0	28.0	37.0
62-63	34.46587912689925	37.0	35.0	37.0	28.0	37.0
64-65	34.55177558566668	37.0	35.0	37.0	28.0	37.0
66-67	34.50434457244611	37.0	35.0	37.0	28.0	37.0
68-69	34.423865182108614	37.0	35.0	37.0	28.0	37.0
70-71	34.383272536763045	37.0	35.0	37.0	28.0	37.0
72-73	34.31445756435345	37.0	35.0	37.0	27.5	37.0
74-75	34.27156386234087	37.0	35.0	37.0	27.0	37.0
76-77	34.23548082467874	37.0	34.5	37.0	27.0	37.0
78-79	34.21373891657663	37.0	34.0	37.0	27.0	37.0
80-81	34.12804562550994	37.0	34.0	37.0	27.0	37.0
82-83	34.06376288011295	37.0	34.0	37.0	26.5	37.0
84-85	34.01838035514355	37.0	34.0	37.0	26.0	37.0
86-87	33.99715950959937	37.0	34.0	37.0	26.0	37.0
88-89	33.903631283285414	37.0	34.0	37.0	26.0	37.0
90-91	33.880061202318586	37.0	34.0	37.0	26.0	37.0
92-93	33.82061472643472	37.0	34.0	37.0	25.5	37.0
94-95	33.76833628447275	37.0	34.0	37.0	25.0	37.0
96-97	33.72196729494654	37.0	34.0	37.0	25.0	37.0
98-99	33.853213365750456	37.0	34.0	37.0	25.5	37.0
100	34.660940622619755	37.0	34.0	37.0	28.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	622.0
21	12711.0
22	64072.0
23	130664.0
24	174573.0
25	214157.0
26	268246.0
27	341857.0
28	441643.0
29	576209.0
30	757046.0
31	1020376.0
32	1441009.0
33	2238920.0
34	4053299.0
35	8622018.0
36	1.2584707E7
37	361838.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	27.003764066394282	29.62675914484741	21.411302421088394	21.958174367669915
2	26.52740550674525	30.417054019887935	25.34706723288796	17.708473240478856
3	18.29448897754662	28.12084692213004	29.231429952691407	24.353234147631937
4	20.69420491615933	29.612585126707742	27.612028675093867	22.081181282039054
5	22.438100457411238	27.878460930157654	27.655343755483287	22.02809485694782
6	22.33948841798999	27.79125252089547	27.39655665365015	22.472702407464386
7	22.792180611884103	28.4893840154292	26.980743776700955	21.737691595985744
8	21.46326590638694	27.63793127083468	28.098368554731795	22.800434268046583
9	22.102894284869844	27.088193603959688	28.099550355609708	22.70936175556076
10-11	22.761678100978784	27.755909559853535	27.09947992193722	22.382932417230464
12-13	22.697990067884273	27.899714507745006	26.935989424884106	22.466305999486615
14-15	22.655807043861486	27.984793120563833	26.931122092285754	22.42827774328893
16-17	22.85042048653044	28.01313734849975	26.881307782334385	22.25513438263543
18-19	22.771708818278423	27.858857113102797	26.856533945010515	22.51290012360826
20-21	22.75623821546533	27.80965040553537	26.94954911283463	22.484562266164673
22-23	22.89313373930041	27.998201482208074	26.811928821355828	22.296735957135684
24-25	22.869466025151937	27.85141035459764	26.797661183940534	22.48146243630989
26-27	22.812514365396066	27.793098778391904	26.910061782424737	22.48432507378729
28-29	22.97355966837022	27.84539561340626	26.77447043643882	22.406574281784696
30-31	22.94925691630743	27.748926619482152	26.72324171587223	22.57857474833819
32-33	22.863037586468156	27.754652262855117	26.824248518752714	22.55806163192401
34-35	22.99114629140662	27.899285091735926	26.703526426538982	22.406042190318473
36-37	22.99515696115914	27.772937039324024	26.680235981282905	22.55167001823393
38-39	22.926553262222345	27.719540701624553	26.780327481689554	22.57357855446355
40-41	23.07575799461887	27.802365497309733	26.66531046663898	22.45656604143241
42-43	23.009025958653844	27.708728541747004	26.659552427100447	22.62269307249871
44-45	22.911606018754025	27.72746994780084	26.7620457959517	22.59887823749343
46-47	23.037199329833285	27.84786040932384	26.69161327982795	22.42332698101492
48-49	23.035086292927023	27.70178703864249	26.66694528591696	22.596181382513524
50-51	22.954381219390392	27.666457561181375	26.762465988826143	22.616695230602087
52-53	23.076895910680946	27.776301597576296	26.64415261845472	22.502649873288032
54-55	23.028824574940064	27.672016832877354	26.656119781931082	22.643038810251504
56-57	22.954167288295167	27.69145862315542	26.75014884535123	22.604225243198194
58-59	23.096073327712503	27.786225415538794	26.661981672999442	22.45571958374926
60-61	23.061379753065665	27.67657485179285	26.618390419822735	22.643654975318746
62-63	22.992435094558083	27.613277012224497	26.736414977887485	22.65787291532994
64-65	23.096885168750113	27.76685671862758	26.636928845377266	22.49932926724504
66-67	23.06358693086662	27.66494077266905	26.643253599931356	22.628218696532972
68-69	22.990612189813127	27.680545018729962	26.715263707358645	22.613579084098266
70-71	23.15187915049047	27.75467030717347	26.61383722221945	22.47961332011661
72-73	23.0855104121645	27.656292093650837	26.606333351110955	22.65186414307371
74-75	23.00293492047242	27.64803924973192	26.700095866351752	22.648929963443905
76-77	23.13183128112893	27.75187648820243	26.608110747666647	22.50818148300199
78-79	23.08893604004807	27.652625223970173	26.608454498987854	22.649984236993898
80-81	23.025975725578444	27.649302086715572	26.68716455289571	22.637557634810275
82-83	23.173751716713014	27.72776076440256	26.58460865256278	22.513878866321647
84-85	23.125639131985128	27.609030536713377	26.572353347386525	22.692976983914974
86-87	23.06352652085976	27.59944576824354	26.670418557598964	22.66660915329774
88-89	23.184622893806445	27.716452062474545	26.56667491281442	22.53225013090459
90-91	23.16350878246848	27.590478103429827	26.561751350174294	22.684261763927395
92-93	23.087070227383588	27.576545335319715	26.65255374850935	22.683830688787353
94-95	23.23629441825685	27.664655245558865	26.531003755197556	22.568046580986724
96-97	23.180128221090975	27.57353269963622	26.50812505628902	22.738214022983776
98-99	23.071430151539964	27.560634997393507	26.62701415054562	22.740920700520906
100	23.54925620142869	27.067853052649042	26.35693235415569	23.02595839176658
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.5
3	1.0
4	1.0
5	0.5
6	0.0
7	1.5
8	9.0
9	23.0
10	50.5
11	160.5
12	439.0
13	679.5
14	1043.0
15	1760.5
16	2633.5
17	4054.0
18	6694.5
19	11070.5
20	18206.0
21	28634.0
22	42241.5
23	60813.0
24	84967.0
25	114455.5
26	150761.0
27	192123.0
28	237040.5
29	287111.0
30	335820.5
31	384292.0
32	440431.0
33	502689.5
34	572207.0
35	646341.5
36	719854.5
37	797273.0
38	878745.5
39	961486.5
40	1050709.0
41	1145438.5
42	1238959.5
43	1321804.5
44	1399728.0
45	1472922.5
46	1529950.0
47	1564326.0
48	1572501.5
49	1561691.5
50	1528918.5
51	1469441.0
52	1385677.0
53	1285935.5
54	1164929.0
55	1023989.5
56	882813.0
57	741410.0
58	605138.0
59	484168.5
60	376439.5
61	285379.5
62	211041.0
63	152410.5
64	109226.5
65	77315.0
66	54508.0
67	38933.0
68	28376.5
69	20706.0
70	15097.5
71	11104.0
72	8338.0
73	6146.5
74	4521.0
75	3366.0
76	2407.5
77	1654.0
78	1190.0
79	826.0
80	547.5
81	364.0
82	238.0
83	168.5
84	106.5
85	66.0
86	45.5
87	23.5
88	12.5
89	7.0
90	3.5
91	1.0
92	1.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12631827756025116
2	0.1807412203632546
3	0.02817982445275517
4	2.5522483521408514E-4
5	0.06308257132956135
6	0.1364702207115314
7	0.03066601461225237
8	0.11926806681810206
9	0.02165507660663508
10-11	0.19926303678699675
12-13	0.06284386104250818
14-15	0.014896122441142075
16-17	0.04156261375333373
18-19	0.09463286492970252
20-21	0.21814366930259874
22-23	0.10982174527006075
24-25	0.055792148977799015
26-27	0.16062199673558428
28-29	0.024698257435923028
30-31	0.1072274598862376
32-33	0.06950673056415588
34-35	0.05074770517592063
36-37	0.0011875461450255374
38-39	0.017512927663366488
40-41	0.07419235827417446
42-43	0.03900436011095254
44-45	0.05258082002178179
46-47	0.05471269805709944
48-49	0.0265133564110632
50-51	0.01497118856914622
52-53	0.09920289080259473
54-55	0.14513435320576956
56-57	0.053275932367100154
58-59	0.0655117112317754
60-61	0.05062609804855391
62-63	0.21448494622367684
64-65	0.03609479698951197
66-67	0.039930677329500196
68-69	0.06966137620042202
70-71	0.04451122061693441
72-73	0.09546912882836048
74-75	0.13243473724784185
76-77	0.1025329104444426
78-79	0.0514173676001283
80-81	0.07952222050637374
82-83	0.11814395934453366
84-85	0.09642442915149535
86-87	0.07344983987580592
88-89	0.08187899663435391
90-91	0.0251105954701837
92-93	0.050012995360858875
94-95	0.024757309579132344
96-97	0.11537760121992306
98-99	0.023693888072075047
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
66	2.0
67	1.0
68	3.0
69	1.0
70	0.0
71	2.0
72	1.0
73	6.0
74	4.0
75	6.0
76	4.0
77	10.0
78	14.0
79	7.0
80	18.0
81	15.0
82	28.0
83	25.0
84	42.0
85	53.0
86	93.0
87	115.0
88	213.0
89	342.0
90	578.0
91	985.0
92	2112.0
93	4020.0
94	8182.0
95	17835.0
96	41195.0
97	104461.0
98	323018.0
99	1797964.0
100	3.1002614E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.07881568631371
#Duplication Level	Percentage of total
1	43.28612464980467
2	10.668159844678858
3	10.225648549114123
4	9.402319596354817
5	7.287401662174258
6	5.419466286175867
7	3.661226233391076
8	2.3457135191327687
9	1.5202205678156608
>10	4.6546030691003075
>50	0.6750371437830319
>100	0.849746650116928
>500	0.004332228357606813
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.702380608149137E-5	1.201058048066283E-5	0.0	3.0026451201657076E-6	0.0	6.005290240331415E-6
2	6.005290240331415E-5	1.201058048066283E-5	0.0	9.007935360497122E-6	3.0026451201657076E-6	1.201058048066283E-5
3	7.206348288397698E-5	2.1018515841159953E-5	0.0	1.8015870720994244E-5	9.007935360497122E-6	1.201058048066283E-5
4	9.308199872513693E-5	3.90343865621542E-5	0.0	2.402116096132566E-5	1.201058048066283E-5	4.503967680248561E-5
5	1.23108449926794E-4	5.404761216298274E-5	0.0	2.702380608149137E-5	1.5013225600828537E-5	4.804232192265132E-5
6	1.3812167552762256E-4	6.906083776381128E-5	0.0	3.302909632182278E-5	2.402116096132566E-5	5.404761216298274E-5
7	1.5313490112845108E-4	7.206348288397698E-5	0.0	3.603174144198849E-5	3.0026451201657075E-5	6.605819264364557E-5
8	1.891666425704396E-4	9.007935360497122E-5	0.0	3.90343865621542E-5	3.302909632182278E-5	6.906083776381128E-5
9	2.1619044865193096E-4	9.608464384530264E-5	0.0	4.2037031682319905E-5	3.90343865621542E-5	7.206348288397698E-5
10-11	2.822486412955765E-4	1.0959654688604833E-4	0.0	4.6540999362568466E-5	4.804232192265132E-5	8.707670848480552E-5
12-13	3.738293174606306E-4	1.2460977248687687E-4	3.0026451201657076E-6	5.404761216298274E-5	6.005290240331415E-5	1.0509257920579977E-4
14-15	4.398875101042762E-4	1.4712961088811968E-4	4.503967680248561E-6	6.1554224963397E-5	6.305554752347985E-5	1.261110950469597E-4
16-17	5.044443801878388E-4	1.876653200103567E-4	6.005290240331415E-6	6.906083776381128E-5	6.755951520372842E-5	1.366203529675397E-4
18-19	5.659986051512358E-4	2.0267854561118527E-4	7.506612800414269E-6	7.506612800414269E-5	8.10714182444741E-5	1.4412696576795395E-4
20-21	6.185448947541358E-4	2.2970235169267664E-4	1.201058048066283E-5	7.80687731243084E-5	9.75859664053855E-5	1.5163357856836824E-4
22-23	6.876057325179471E-4	2.6423277057458227E-4	1.201058048066283E-5	8.10714182444741E-5	1.1710315968646259E-4	1.6364415904903106E-4
24-25	7.491599574813441E-4	2.9125657665607363E-4	1.201058048066283E-5	8.407406336463981E-5	1.3211638528729113E-4	1.7415341696961104E-4
26-27	8.437432787665639E-4	3.272883180980621E-4	1.5013225600828537E-5	8.407406336463981E-5	1.4412696576795395E-4	1.8466267489019103E-4
28-29	9.007935360497122E-4	3.4530418881905636E-4	2.402116096132566E-5	8.857803104488837E-5	1.591401913687825E-4	2.0267854561118527E-4
30-31	9.833662768542692E-4	3.6181873697996775E-4	2.5522483521408517E-5	9.908728896546834E-5	1.8316135233010817E-4	2.2669970657251091E-4
32-33	0.0010479231469378318	3.7082667234046487E-4	3.0026451201657078E-5	1.0659390176588263E-4	2.0267854561118527E-4	2.5822748033425087E-4
34-35	0.0011109786944613117	3.8133593026104485E-4	3.302909632182278E-5	1.1109786944613118E-4	2.176917712120138E-4	2.972618668964051E-4
36-37	0.001171031596864626	3.9634915586187337E-4	3.302909632182278E-5	1.23108449926794E-4	2.3871028705317376E-4	3.603174144198849E-4
38-39	0.0012370897895082715	4.143650265828676E-4	3.302909632182278E-5	1.3361770784737397E-4	2.5822748033425087E-4	5.164549606685017E-4
40-41	0.0013271691431132428	4.488954454647733E-4	3.603174144198849E-5	1.366203529675397E-4	2.8074731873549365E-4	5.494840569903245E-4
42-43	0.0014022352711173854	4.6691131618576754E-4	3.90343865621542E-5	1.3812167552762256E-4	3.18280382737565E-4	5.720038953915673E-4
44-45	0.0014742987540013624	4.909324771470932E-4	3.90343865621542E-5	1.3812167552762256E-4	3.483068339392221E-4	5.96025056352893E-4
46-47	0.001531349011284511	5.059457027479218E-4	4.053570912223705E-5	1.4863093344820254E-4	3.6181873697996775E-4	6.2605150755455E-4
48-49	0.0016244310100096478	5.194576057886674E-4	4.353835424240276E-5	1.561375462486168E-4	3.90343865621542E-4	6.515739910759586E-4
50-51	0.0017040011056940392	5.404761216298273E-4	4.503967680248561E-5	1.591401913687825E-4	4.398875101042762E-4	6.891070550780298E-4
52-53	0.0017685579757776018	5.554893472306558E-4	5.104496704281703E-5	1.6514548160911392E-4	4.7892189666643033E-4	7.071229257990242E-4
54-55	0.001849629394022076	5.599933149109044E-4	5.254628960289988E-5	1.7415341696961104E-4	5.194576057886674E-4	7.431546672410127E-4
56-57	0.0019156875866657213	5.75006540511733E-4	5.5548934723065595E-5	1.756547395296939E-4	5.690012502714016E-4	7.656745056422555E-4
58-59	0.0020117722305110237	5.930224112327272E-4	5.7050257283148444E-5	1.816600297700253E-4	6.245501849944672E-4	8.107141824447411E-4
60-61	0.002061315874993758	5.990277014730587E-4	5.7050257283148444E-5	1.8466267489019103E-4	6.6808853923687E-4	8.542525366871437E-4
62-63	0.002137883325557984	6.245501849944672E-4	5.7050257283148444E-5	1.891666425704396E-4	7.461573123611784E-4	8.812763427686352E-4
64-65	0.0022009388730814634	6.530753136360414E-4	6.1554224963397E-5	2.0267854561118527E-4	8.047088922044096E-4	9.143054390904579E-4
66-67	0.002279007646205772	6.620832489965386E-4	6.906083776381128E-5	2.0718251329143383E-4	8.572551818073096E-4	9.428305677320321E-4
68-69	0.002375092290051075	6.921097001981956E-4	7.356480544405984E-5	2.146891260918481E-4	9.24814697011038E-4	9.713556963736065E-4
70-71	0.00240962270893298	7.206348288397698E-4	7.506612800414269E-5	2.2369706145234522E-4	0.0010043847926954291	0.0010103900829357606
72-73	0.002496699417417786	7.491599574813441E-4	7.506612800414269E-5	2.2519838401242808E-4	0.0010794509206995717	0.0010434191792575835
74-75	0.002555250997261017	7.701784733225041E-4	7.506612800414269E-5	2.342063193729252E-4	0.0011830421773452887	0.001088458856060069
76-77	0.0026318184478252428	7.791864086830011E-4	7.957009568439125E-5	2.3570764193300804E-4	0.0012866334339910056	0.0011319972103024717
78-79	0.0026843647374281427	7.911969891636639E-4	8.257274080455696E-5	2.372089644930909E-4	0.0013797154327161425	0.001180039532225123
80-81	0.0027564282203121192	8.137168275649067E-4	9.158067616505408E-5	2.372089644930909E-4	0.0014983199149626882	0.0012205752413473603
82-83	0.0028119771550351854	8.272287306056524E-4	9.308199872513693E-5	2.372089644930909E-4	0.0016214283648894822	0.0012611109504695973
84-85	0.002882539315359079	8.42241956206481E-4	9.458332128521978E-5	2.402116096132566E-4	0.0017700592983376847	0.0013121559175124143
86-87	0.0029726186689640506	8.617591494875581E-4	1.0809522432596548E-4	2.432142547334223E-4	0.0018886637805842301	0.0013586969168749826
88-89	0.0030206609908867018	8.827776653287181E-4	1.0809522432596548E-4	2.5822748033425087E-4	0.002005766940270693	0.0014007339485573025
>>END_MODULE
