##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674172
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1443784
Total Bases	144.3 Mbp
Sequences flagged as poor quality	0
Sequence length	82-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.308583555434886	37.0	36.0	37.0	31.0	37.0
2	34.8934812963712	37.0	35.0	37.0	29.0	37.0
3	35.19750946124905	37.0	35.0	37.0	31.0	37.0
4	34.889229967917636	37.0	35.0	37.0	30.0	37.0
5	35.01206482410111	37.0	35.0	37.0	30.0	37.0
6	34.91615712599668	37.0	35.0	37.0	30.0	37.0
7	34.74252311980185	37.0	35.0	37.0	30.0	37.0
8	34.99256398464036	37.0	35.0	37.0	30.0	37.0
9	35.016741423924906	37.0	35.0	37.0	30.0	37.0
10-11	34.98887402824799	37.0	35.0	37.0	30.0	37.0
12-13	34.92692189413375	37.0	35.0	37.0	29.5	37.0
14-15	34.95288976744444	37.0	35.0	37.0	30.0	37.0
16-17	34.635343998825306	37.0	35.0	37.0	29.0	37.0
18-19	33.776937547444774	37.0	34.5	37.0	27.0	37.0
20-21	33.90786849002343	37.0	35.0	37.0	27.0	37.0
22-23	34.62740686972567	37.0	35.0	37.0	29.0	37.0
24-25	34.86263804003923	37.0	35.0	37.0	29.5	37.0
26-27	34.43650781557352	37.0	35.0	37.0	28.5	37.0
28-29	34.64942124306683	37.0	35.0	37.0	29.0	37.0
30-31	34.81684517905725	37.0	35.0	37.0	29.0	37.0
32-33	33.922196810603246	37.0	35.0	37.0	27.0	37.0
34-35	33.566757908385185	37.0	34.5	37.0	25.0	37.0
36-37	33.57626590958205	37.0	34.0	37.0	25.0	37.0
38-39	34.68041237470425	37.0	35.0	37.0	29.0	37.0
40-41	34.59273305425188	37.0	35.0	37.0	28.5	37.0
42-43	34.80066270300821	37.0	35.0	37.0	29.0	37.0
44-45	34.72496924747746	37.0	35.0	37.0	29.0	37.0
46-47	34.114600937536366	37.0	34.5	37.0	27.5	37.0
48-49	34.72015377646518	37.0	35.0	37.0	29.0	37.0
50-51	34.74313228294537	37.0	35.0	37.0	29.0	37.0
52-53	33.526510890825776	37.0	34.0	37.0	25.0	37.0
54-55	34.262837100286475	37.0	34.5	37.0	28.0	37.0
56-57	34.42658112293806	37.0	35.0	37.0	28.5	37.0
58-59	32.8232595734542	37.0	34.0	37.0	21.5	37.0
60-61	34.6433244169488	37.0	35.0	37.0	29.0	37.0
62-63	34.47493288469744	37.0	35.0	37.0	28.5	37.0
64-65	34.638031035113286	37.0	35.0	37.0	29.0	37.0
66-67	34.62742314639863	37.0	35.0	37.0	29.0	37.0
68-69	34.55415907088595	37.0	35.0	37.0	28.5	37.0
70-71	34.59817015564655	37.0	35.0	37.0	29.0	37.0
72-73	33.989238002360466	37.0	34.5	37.0	27.0	37.0
74-75	33.588665271259416	37.0	34.0	37.0	26.0	37.0
76-77	34.40849323721554	37.0	35.0	37.0	28.0	37.0
78-79	34.249239498429134	37.0	34.5	37.0	27.5	37.0
80-81	33.9113267635602	37.0	34.5	37.0	26.5	37.0
82-83	34.45200647612255	37.0	35.0	37.0	28.0	37.0
84-85	33.8222268847517	37.0	34.0	37.0	26.0	37.0
86-87	34.2369833097956	37.0	34.5	37.0	27.5	37.0
88-89	34.32843237455425	37.0	34.5	37.0	27.5	37.0
90-91	34.38846469120463	37.0	34.0	37.0	28.0	37.0
92-93	33.86278693576128	37.0	34.0	37.0	26.0	37.0
94-95	33.63353980804567	37.0	34.0	37.0	25.5	37.0
96-97	34.381839286372255	37.0	34.0	37.0	28.0	37.0
98-99	34.249246265113726	37.0	34.0	37.0	27.5	37.0
100	34.95259970841955	37.0	35.0	37.0	29.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	10.0
21	126.0
22	578.0
23	1648.0
24	3306.0
25	5726.0
26	9529.0
27	14891.0
28	22691.0
29	32996.0
30	47227.0
31	67066.0
32	96007.0
33	143323.0
34	230042.0
35	398111.0
36	362649.0
37	7858.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	28.049903993483195	26.676789143089884	23.230875584974772	22.04243127845215
2	26.078729337254515	30.00653838321852	25.79205170863745	18.12268057088951
3	19.35106501609316	26.942659586183776	29.607537431230913	24.09873796649215
4	22.112254354489934	28.16982126358135	27.94757139602271	21.770352985906
5	22.50896951059026	26.6443640997936	28.233318095554726	22.61334829406142
6	22.379265809674543	27.296745135311447	27.436781059378696	22.88720799563532
7	23.092821117845723	28.146527140972022	27.05556238956856	21.705089351613697
8	21.803776470408966	27.00317637981357	27.999933507967267	23.19311364181019
9	22.534124509872505	26.615559266552474	27.82975251375405	23.020563709820973
10-11	22.969062439611605	27.140684273701172	27.071490858365443	22.818762428321786
12-13	22.919774106780157	27.141194344427248	26.930151037606503	23.008880511186092
14-15	22.93444920550211	27.24367776524503	26.952565620576912	22.869307408675947
16-17	23.074460136084443	27.35943909627847	26.888318670491685	22.677782097145403
18-19	23.15545334616893	27.219203479873073	26.707990159375335	22.917353014582666
20-21	23.085108760310174	27.09336505885929	26.829635982932633	22.9918901978979
22-23	23.124108744172254	27.291062491779044	26.757927087704793	22.826901676343905
24-25	23.14364446297609	27.25549972589248	26.79140434824919	22.80945146288224
26-27	23.065219390590048	27.074112578272608	26.98218040456451	22.878487626572838
28-29	23.220945507780428	27.255955346382816	26.81102647098934	22.712072674847413
30-31	23.22089972055808	27.173570868777276	26.688036637401858	22.917492773262786
32-33	23.156668950966996	27.001352135537044	26.79718392590354	23.044794987592418
34-35	23.163471960750755	27.122946260482628	26.791923384106337	22.921658394660277
36-37	23.283502864790677	27.13347781472603	26.601855833684603	22.981163486798685
38-39	23.145352127523815	27.03198776028807	26.89875744175595	22.923902670432163
40-41	23.2239199245012	27.25090545668145	26.69103256860174	22.83414205021561
42-43	23.275758473028127	27.092634054187897	26.693471443735078	22.9381360290489
44-45	23.166205951229042	27.030155100989145	26.81987215406343	22.983766793718384
46-47	23.19815753665714	27.120733051451708	26.838898259185445	22.842211152705705
48-49	23.26658198073649	27.09410666271404	26.788897923901793	22.850413432647674
50-51	23.177974393743387	27.002703325211236	26.877494860911234	22.941827420134146
52-53	23.295170644319484	27.153512302447364	26.703598914786795	22.847718138446357
54-55	23.281295726958334	27.006024726167087	26.740994116530253	22.971685430344326
56-57	23.126373054668846	26.94908949053995	26.95378741242291	22.970750042368294
58-59	23.243023522580724	27.112285719168085	26.769029177714977	22.875661580536217
60-61	23.313080814062616	27.052662783779425	26.783298736433963	22.850957665723996
62-63	23.233663694299427	26.977433761953606	26.84587624059334	22.943026303153623
64-65	23.21397588609009	27.128685440400062	26.804292227480435	22.853046446029413
66-67	23.277004621621014	27.087374437881707	26.701090730264603	22.934530210232673
68-69	23.144624027403285	26.959679556116644	26.934603748556153	22.96109266792392
70-71	23.28121028174292	27.130249216803588	26.82881470158191	22.759725799871585
72-73	23.23454867783943	27.084986048681703	26.746303831676343	22.93416144180252
74-75	23.1818742043106	26.99591139430787	26.856276141536	22.96593825984553
76-77	23.20227862795733	27.104203088451502	26.835223500870647	22.85829478272052
78-79	23.22069535076105	27.036455437980813	26.76225866892462	22.980590542333516
80-81	23.20437758535241	26.91291930697364	26.92373752611915	22.958965581554796
82-83	23.227255836536457	27.135214956106278	26.831288746176245	22.80624046118101
84-85	23.263786180269875	27.001907599130163	26.775525116721884	22.95878110387808
86-87	23.175412480574874	26.906914909345943	26.89494760652204	23.02272500355714
88-89	23.25334694646548	27.083633487196824	26.8258590403573	22.837160525980398
90-91	23.27597850336725	26.98898917480109	26.806791428775263	22.928240893056397
92-93	23.21085399445167	26.944038567264865	26.90419199937088	22.94091543891258
94-95	23.29178127484154	27.053344682766916	26.78415909842351	22.870714943968036
96-97	23.232823208541998	27.069808652978207	26.782625321873248	22.914742816606545
98-99	23.138744253854203	26.929822173929125	26.922145525525902	23.00928804669077
100	23.622869003897154	26.602913921481857	26.5641668917857	23.210050182835282
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	0.0
7	0.5
8	2.5
9	4.5
10	6.5
11	11.5
12	23.5
13	45.5
14	72.5
15	114.5
16	167.0
17	256.0
18	415.5
19	653.0
20	989.5
21	1464.0
22	2115.0
23	3037.0
24	4255.0
25	5654.5
26	7251.0
27	9017.0
28	11114.0
29	13446.5
30	15877.5
31	18430.0
32	20782.5
33	23235.0
34	25757.5
35	28157.5
36	30681.5
37	33295.5
38	36200.0
39	39159.5
40	42280.0
41	45687.0
42	49686.5
43	53970.5
44	57766.0
45	61342.0
46	64578.0
47	67416.0
48	69055.0
49	68755.5
50	66761.5
51	63574.0
52	59002.0
53	53127.5
54	47069.5
55	40947.5
56	34811.0
57	29442.5
58	24860.0
59	20749.0
60	17164.5
61	14202.5
62	11814.0
63	9871.5
64	8249.5
65	6778.0
66	5489.0
67	4433.0
68	3594.0
69	2786.5
70	2058.0
71	1545.5
72	1144.0
73	790.0
74	526.5
75	383.0
76	266.5
77	154.0
78	89.0
79	63.0
80	39.0
81	22.0
82	16.0
83	10.0
84	2.0
85	1.0
86	2.5
87	3.5
88	2.5
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00914264183562084
2	1.3852487629728547E-4
3	2.077873144459282E-4
4	0.897779723282707
5	2.7704975259457094E-4
6	0.08983338227878962
7	0.902628093953112
8	1.3852487629728547E-4
9	0.001870085830013354
10-11	3.4631219074321364E-4
12-13	3.1168097166889226E-4
14-15	7.272556005607487E-4
16-17	0.49193646695073506
18-19	2.869819862250863
20-21	2.368325178835615
22-23	0.47839566029267533
24-25	5.194682861148206E-4
26-27	0.9645140824389244
28-29	0.4563009425232583
30-31	9.35042915006677E-4
32-33	2.3661434120339333
34-35	2.8718977353953226
36-37	2.3968959345719307
38-39	0.13298388124539406
40-41	0.4806813207515806
42-43	7.965180387093915E-4
44-45	0.0010389365722296411
46-47	1.4029106847007584
48-49	0.001246723886675569
50-51	0.013263756905465083
52-53	2.8984598804253268
54-55	0.9530857801443983
56-57	0.48334792462030335
58-59	4.749567802385952
60-61	9.004116959323555E-4
62-63	0.4689067062663113
64-65	7.965180387093915E-4
66-67	0.0018354546109390325
68-69	0.0111858837610058
70-71	0.0014545112011214974
72-73	1.428260737063162
74-75	2.779293855590587
76-77	0.3600954159347936
78-79	0.4498595357754345
80-81	1.4032223656724274
82-83	8.311495456209328E-4
84-85	1.4217861762419202
86-87	0.45238855477388884
88-89	0.005194700851030525
90-91	8.657867733408322E-4
92-93	1.3541686868490972
94-95	1.5393076353773048
96-97	6.927544123260407E-4
98-99	0.4502922574950578
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
82	1.0
83	0.0
84	1.0
85	1.0
86	0.0
87	0.0
88	4.0
89	2.0
90	3.0
91	3.0
92	10.0
93	11.0
94	29.0
95	77.0
96	258.0
97	1079.0
98	5813.0
99	55741.0
100	1380751.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.27691323728408
#Duplication Level	Percentage of total
1	96.6738532232364
2	3.0138418514021414
3	0.2378415045493772
4	0.04546595724354235
5	0.02065172138653789
6	0.007119183068824749
7	0.0012265591131589944
8	0.0
9	0.0
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	6.926243814864274E-5	0.0
2	0.0	0.0	0.0	0.0	3.463121907432137E-4	0.0
3	0.0	0.0	0.0	0.0	4.155746288918564E-4	0.0
4	0.0	0.0	0.0	0.0	5.540995051891419E-4	0.0
5	0.0	6.926243814864274E-5	0.0	0.0	6.233619433377846E-4	0.0
6	0.0	6.926243814864274E-5	0.0	0.0	7.6188681963507E-4	0.0
7	0.0	6.926243814864274E-5	6.926243814864274E-5	0.0	9.004116959323555E-4	0.0
8	0.0	6.926243814864274E-5	6.926243814864274E-5	0.0	9.696741340809983E-4	0.0
9	0.0	6.926243814864274E-5	6.926243814864274E-5	0.0	9.696741340809983E-4	0.0
10-11	0.0	6.926243814864274E-5	6.926243814864274E-5	0.0	0.001142830229452605	0.0
12-13	0.0	6.926243814864274E-5	6.926243814864274E-5	0.0	0.0011774614485269264	1.3852487629728547E-4
14-15	0.0	6.926243814864274E-5	6.926243814864274E-5	0.0	0.0012467238866755693	1.3852487629728547E-4
16-17	0.0	6.926243814864274E-5	6.926243814864274E-5	0.0	0.0012467238866755693	1.3852487629728547E-4
18-19	0.0	6.926243814864274E-5	6.926243814864274E-5	0.0	0.0013159863248242121	1.7315609537160682E-4
20-21	0.0	6.926243814864274E-5	6.926243814864274E-5	0.0	0.0014891424201958188	2.077873144459282E-4
22-23	0.0	6.926243814864274E-5	6.926243814864274E-5	0.0	0.0016276672964931041	2.077873144459282E-4
24-25	0.0	6.926243814864274E-5	6.926243814864274E-5	6.926243814864274E-5	0.001696929734641747	2.7704975259457094E-4
26-27	0.0	6.926243814864274E-5	6.926243814864274E-5	6.926243814864274E-5	0.0020086107063106394	4.155746288918564E-4
28-29	0.0	6.926243814864274E-5	6.926243814864274E-5	6.926243814864274E-5	0.0020778731444592823	5.540995051891419E-4
30-31	0.0	6.926243814864274E-5	6.926243814864274E-5	6.926243814864274E-5	0.002181766801682246	5.887307242634632E-4
32-33	0.0	6.926243814864274E-5	6.926243814864274E-5	6.926243814864274E-5	0.0023895541161281742	6.233619433377846E-4
34-35	0.0	6.926243814864274E-5	6.926243814864274E-5	6.926243814864274E-5	0.0025973414305741024	6.233619433377846E-4
36-37	0.0	1.038936572229641E-4	1.3852487629728547E-4	6.926243814864274E-5	0.002770497525945709	7.272556005607487E-4
38-39	0.0	1.3852487629728547E-4	1.3852487629728547E-4	6.926243814864274E-5	0.002839759964094352	7.965180387093913E-4
40-41	0.0	1.3852487629728547E-4	1.3852487629728547E-4	6.926243814864274E-5	0.002839759964094352	8.311492577837128E-4
42-43	0.0	1.3852487629728547E-4	1.3852487629728547E-4	6.926243814864274E-5	0.0030821784976146015	9.004116959323555E-4
44-45	0.0	1.3852487629728547E-4	1.3852487629728547E-4	6.926243814864274E-5	0.0032207033739118872	9.350429150066768E-4
46-47	0.0	2.077873144459282E-4	1.3852487629728547E-4	6.926243814864274E-5	0.0032899658120605297	0.0010389365722296411
48-49	0.0	2.077873144459282E-4	1.3852487629728547E-4	6.926243814864274E-5	0.003393859469283494	0.0011081990103782838
50-51	0.0	2.077873144459282E-4	1.3852487629728547E-4	6.926243814864274E-5	0.0034284906883578154	0.0011081990103782838
52-53	0.0	2.077873144459282E-4	1.3852487629728547E-4	6.926243814864274E-5	0.0035323843455807797	0.0011774614485269264
54-55	0.0	2.077873144459282E-4	1.3852487629728547E-4	6.926243814864274E-5	0.003740171660026708	0.0012467238866755693
56-57	0.0	2.077873144459282E-4	1.3852487629728547E-4	6.926243814864274E-5	0.003774802879101029	0.0012813551057498907
58-59	0.0	2.077873144459282E-4	1.3852487629728547E-4	6.926243814864274E-5	0.003878696536323993	0.001315986324824212
60-61	0.0	2.077873144459282E-4	1.3852487629728547E-4	6.926243814864274E-5	0.0039133277553983145	0.0014545112011214974
62-63	0.0	2.077873144459282E-4	1.3852487629728547E-4	6.926243814864274E-5	0.004017221412621279	0.00152377363927014
64-65	3.463121907432137E-5	2.077873144459282E-4	2.077873144459282E-4	6.926243814864274E-5	0.004190377507992885	0.0015584048583444615
66-67	6.926243814864274E-5	2.077873144459282E-4	2.077873144459282E-4	6.926243814864274E-5	0.004398164822438814	0.001593036077418783
68-69	6.926243814864274E-5	2.077873144459282E-4	2.077873144459282E-4	6.926243814864274E-5	0.004536689698736099	0.001593036077418783
70-71	6.926243814864274E-5	2.4241853352024957E-4	2.077873144459282E-4	6.926243814864274E-5	0.004744477013182027	0.001696929734641747
72-73	6.926243814864274E-5	2.7704975259457094E-4	2.077873144459282E-4	6.926243814864274E-5	0.004883001889479313	0.0017315609537160684
74-75	6.926243814864274E-5	2.7704975259457094E-4	2.077873144459282E-4	6.926243814864274E-5	0.004917633108553634	0.001870085830013354
76-77	6.926243814864274E-5	2.7704975259457094E-4	2.077873144459282E-4	6.926243814864274E-5	0.005090789203925241	0.0019393482681619965
78-79	6.926243814864274E-5	2.7704975259457094E-4	2.077873144459282E-4	1.038936572229641E-4	0.005160051642073883	0.002077873144459282
80-81	6.926243814864274E-5	2.7704975259457094E-4	2.077873144459282E-4	1.3852487629728547E-4	0.005263945299296848	0.002077873144459282
82-83	6.926243814864274E-5	3.116809716688923E-4	2.077873144459282E-4	1.3852487629728547E-4	0.005263945299296848	0.002077873144459282
84-85	6.926243814864274E-5	3.463121907432137E-4	2.077873144459282E-4	2.077873144459282E-4	0.0053332077374454905	0.0021471355826079247
86-87	6.926243814864274E-5	3.463121907432137E-4	2.077873144459282E-4	2.077873144459282E-4	0.005402470175594133	0.0022163980207565675
88-89	6.926243814864274E-5	3.463121907432137E-4	2.077873144459282E-4	2.077873144459282E-4	0.005471732613742776	0.0022856604589052104
>>END_MODULE
