##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674181
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49849976
Total Bases	4.9 Gbp
Sequences flagged as poor quality	0
Sequence length	62-100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.26339641567731	37.0	36.0	37.0	31.0	37.0
2	34.90248221985102	37.0	35.0	37.0	29.0	37.0
3	35.04671566943182	37.0	35.0	37.0	30.0	37.0
4	35.09044941165067	37.0	35.0	37.0	30.0	37.0
5	35.07348190097424	37.0	35.0	37.0	30.0	37.0
6	34.88524187052768	37.0	35.0	37.0	29.0	37.0
7	35.03004047584697	37.0	35.0	37.0	30.0	37.0
8	35.02349740348922	37.0	35.0	37.0	30.0	37.0
9	34.95733737163685	37.0	35.0	37.0	30.0	37.0
10-11	34.99476343779985	37.0	35.0	37.0	30.0	37.0
12-13	34.97304032804349	37.0	35.0	37.0	30.0	37.0
14-15	35.01367020718325	37.0	35.0	37.0	30.0	37.0
16-17	34.95321062942939	37.0	35.0	37.0	30.0	37.0
18-19	34.961078286176104	37.0	35.0	37.0	30.0	37.0
20-21	34.8728133590275	37.0	35.0	37.0	29.5	37.0
22-23	34.86534388702614	37.0	35.0	37.0	29.5	37.0
24-25	34.928575261500626	37.0	35.0	37.0	29.5	37.0
26-27	34.913826839154346	37.0	35.0	37.0	29.0	37.0
28-29	34.93256285619877	37.0	35.0	37.0	30.0	37.0
30-31	34.93740642924281	37.0	35.0	37.0	30.0	37.0
32-33	34.91205414020661	37.0	35.0	37.0	29.0	37.0
34-35	34.773505608107016	37.0	35.0	37.0	29.0	37.0
36-37	34.88269805786867	37.0	35.0	37.0	29.0	37.0
38-39	34.7949504930554	37.0	35.0	37.0	29.0	37.0
40-41	34.84349534090047	37.0	35.0	37.0	29.0	37.0
42-43	34.87677899784746	37.0	35.0	37.0	29.0	37.0
44-45	34.85932151702541	37.0	35.0	37.0	29.0	37.0
46-47	34.78193854095336	37.0	35.0	37.0	29.0	37.0
48-49	34.79133196573655	37.0	35.0	37.0	29.0	37.0
50-51	34.81468202712876	37.0	35.0	37.0	29.0	37.0
52-53	34.75832827883408	37.0	35.0	37.0	29.0	37.0
54-55	34.717994859215175	37.0	35.0	37.0	29.0	37.0
56-57	34.75723728532989	37.0	35.0	37.0	29.0	37.0
58-59	34.613628580282565	37.0	35.0	37.0	28.5	37.0
60-61	34.69228095516034	37.0	35.0	37.0	29.0	37.0
62-63	34.7044128408086	37.0	35.0	37.0	29.0	37.0
64-65	34.64793032084491	37.0	35.0	37.0	29.0	37.0
66-67	34.67870395867922	37.0	35.0	37.0	29.0	37.0
68-69	34.62154103189245	37.0	35.0	37.0	28.5	37.0
70-71	34.61965569135636	37.0	35.0	37.0	28.5	37.0
72-73	34.622197853869544	37.0	35.0	37.0	28.5	37.0
74-75	34.59812526813654	37.0	35.0	37.0	28.0	37.0
76-77	34.574172192307685	37.0	35.0	37.0	28.0	37.0
78-79	34.463483610093206	37.0	35.0	37.0	28.0	37.0
80-81	34.51278094213113	37.0	35.0	37.0	28.0	37.0
82-83	34.417861337798186	37.0	35.0	37.0	28.0	37.0
84-85	34.365692567669456	37.0	34.0	37.0	28.0	37.0
86-87	34.304504737442095	37.0	34.0	37.0	27.5	37.0
88-89	34.29691941451328	37.0	34.0	37.0	27.0	37.0
90-91	34.244583753208666	37.0	34.0	37.0	27.0	37.0
92-93	34.19773475836051	37.0	34.0	37.0	27.0	37.0
94-95	34.06305614431892	37.0	34.0	37.0	26.5	37.0
96-97	34.113359814864715	37.0	34.0	37.0	27.0	37.0
98-99	34.194538086195905	37.0	34.0	37.0	27.0	37.0
100	34.90868627865816	37.0	34.0	37.0	29.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	326.0
20	14049.0
21	70463.0
22	132751.0
23	200455.0
24	272020.0
25	347726.0
26	449627.0
27	582838.0
28	754101.0
29	980555.0
30	1268992.0
31	1664580.0
32	2250263.0
33	3218436.0
34	5144937.0
35	1.0467264E7
36	2.0924073E7
37	1106520.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	24.414203621080187	29.93922388406674	20.185452584450918	25.461119910402157
2	28.15854094391405	29.848811132293402	24.182284836843333	17.810363086949216
3	16.360813246630183	27.181351828799492	30.94416755978869	25.513667364781632
4	19.7040379479002	28.668312474837066	29.483321519542656	22.144328057720077
5	22.304126949823512	26.41211681086969	28.59599460908479	22.68776163022201
6	21.745362088315012	26.256834438314563	28.767475725605706	23.23032774776472
7	23.236915800664814	27.787176428958738	27.383364408813847	21.592543361562598
8	20.80207075563202	25.286591544334257	29.414732287671015	24.496605412362708
9	22.36774737764962	25.032188075280786	28.96843728729378	23.631627259775815
10-11	23.48317290844815	25.9434745325683	26.843759911933496	23.729592647050055
12-13	23.587368315084866	26.270687260718162	26.348995261569552	23.792949162627423
14-15	23.67516113935866	26.309924985686433	26.227583224266827	23.787330650688084
16-17	23.672923491084372	26.379071889257578	26.365128405500787	23.582876214157263
18-19	23.66356324776837	26.37911814997774	26.301732473315926	23.655586128937966
20-21	23.71884581024781	26.199546086007103	26.313264717405556	23.768343386339534
22-23	23.718632551706715	26.364446222942757	26.247911872576267	23.66900935277426
24-25	23.659500500119588	26.371350342148748	26.294399836628806	23.674749321102865
26-27	23.761320191912652	26.16380846803962	26.289856816056318	23.785014523991414
28-29	23.796130195694364	26.270467300195648	26.218738187440017	23.714664316669968
30-31	23.74818726117743	26.27519555633287	26.203521014500435	23.773096167989262
32-33	23.821455553982904	26.12212614332433	26.195421827533615	23.860996475159148
34-35	23.824776312976272	26.292140588262626	26.152265760884593	23.730817337876513
36-37	23.764137452622947	26.273718431219418	26.209202668625064	23.75294144753257
38-39	23.840831973368797	26.105792398157135	26.17654248038616	23.876833148087904
40-41	23.851325398790202	26.2146890443351	26.16208590260297	23.771899654271728
42-43	23.777868318699582	26.237282702999643	26.173473987800566	23.81137499050021
44-45	23.83376846906746	26.112139634426594	26.18341516160302	23.870676734902922
46-47	23.846757264594885	26.238220482638567	26.16654650017366	23.74847575259289
48-49	23.785545281907183	26.231005320978657	26.194996712576245	23.788452684537916
50-51	23.860847461337784	26.07619083968685	26.161917358716597	23.901044340258778
52-53	23.849117102362875	26.18665822893797	26.19350766039752	23.770717008301634
54-55	23.78897199510933	26.203488933730945	26.19530273846355	23.812236332696173
56-57	23.859992133444806	26.08279765059656	26.18212991647409	23.875080299484544
58-59	23.849637927535028	26.18353937442216	26.21218018097012	23.754642517072693
60-61	23.817319461689877	26.208310274028012	26.177279524685122	23.79709073959699
62-63	23.855454462428998	26.064310431113576	26.17662874597691	23.903606360480516
64-65	23.87279717961901	26.20834839839869	26.14832737121452	23.770527050767782
66-67	23.798291067331117	26.225446004067987	26.18787911580046	23.788383812800433
68-69	23.86182543943797	26.07666248844905	26.17356332177554	23.887948750337443
70-71	23.88405636227312	26.19289187928551	26.17446659969356	23.748585158747805
72-73	23.81505277926645	26.20101785001014	26.178246190840355	23.80568317988306
74-75	23.87570860480078	26.057184463348886	26.175054956239553	23.89205197561078
76-77	23.875669916420946	26.180578550819234	26.163189842988317	23.78056168977151
78-79	23.805765776466167	26.193882020084235	26.20317496588123	23.797177237568363
80-81	23.88676862458003	26.037695057835712	26.184619153545892	23.890917164038367
82-83	23.905306885882485	26.176822179174586	26.147242509917373	23.77062842502556
84-85	23.846543423431335	26.177271066000362	26.1679951041383	23.808190406430008
86-87	23.90533380669417	26.04564766791593	26.152090563955966	23.896927961433935
88-89	23.914362271970155	26.15583873063126	26.152342000184596	23.77745699721398
90-91	23.878500728655595	26.15466695789611	26.16730288821666	23.799529425231636
92-93	23.930773490561787	26.006002431315263	26.158094699426243	23.905129378696703
94-95	23.964686826839536	26.117919366206106	26.128059809792802	23.78933399716156
96-97	23.885606926670686	26.157337121377296	26.147779933079583	23.80927601887243
98-99	23.93731995982799	25.979477344942392	26.14480751082229	23.938395184407327
100	24.293580593701723	25.559554204902547	25.952561711087412	24.194303490308318
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	18.0
2	17.0
3	25.0
4	43.5
5	74.0
6	127.0
7	186.5
8	286.5
9	536.0
10	1112.5
11	2130.0
12	3903.0
13	6854.0
14	11382.0
15	18465.5
16	28293.5
17	41311.0
18	58035.5
19	78643.0
20	103233.5
21	132115.5
22	164889.5
23	201513.5
24	240740.5
25	281258.0
26	323823.5
27	370702.0
28	421235.5
29	472415.0
30	525155.5
31	581890.5
32	641172.0
33	704674.0
34	778138.0
35	858793.0
36	942151.5
37	1028378.5
38	1116798.0
39	1206994.0
40	1297825.5
41	1382345.5
42	1460123.0
43	1531362.5
44	1590669.0
45	1636970.0
46	1673662.5
47	1702730.0
48	1722675.0
49	1729836.5
50	1726148.5
51	1715620.0
52	1689414.0
53	1648155.5
54	1599697.0
55	1536759.0
56	1456348.0
57	1370779.0
58	1281432.5
59	1188773.0
60	1096064.5
61	1000914.0
62	904722.5
63	807784.0
64	713518.5
65	620496.0
66	530009.0
67	444451.0
68	364244.5
69	291417.5
70	226161.0
71	171688.5
72	127408.0
73	91523.5
74	64030.0
75	43508.0
76	28977.0
77	18970.0
78	12125.0
79	7609.5
80	4586.0
81	2813.0
82	1758.5
83	1076.5
84	676.0
85	454.5
86	317.5
87	221.0
88	150.5
89	106.0
90	85.0
91	62.0
92	45.5
93	35.0
94	24.0
95	20.5
96	17.5
97	12.0
98	9.5
99	8.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.040529608279049126
2	0.002407222823938772
3	9.989974719345903E-4
4	0.05951858432188613
5	0.04800002310933911
6	0.3148948356564906
7	0.030896704945254136
8	0.0030210646440431586
9	0.2383672160644571
10-11	0.07911137208972778
12-13	0.12685462476451342
14-15	8.485460454384171E-4
16-17	0.01903812350882576
18-19	0.014882655109001457
20-21	0.17236317225107592
22-23	0.22093390777159053
24-25	0.03990774238286494
26-27	0.03910433176537537
28-29	0.01531896424584036
30-31	6.770314192327796E-4
32-33	0.031148460332257735
34-35	0.277605951104169
36-37	0.048481467674126866
38-39	0.20102416899859693
40-41	0.09020265125102568
42-43	4.0621885153966774E-4
44-45	0.015010037316768216
46-47	0.14963297073603404
48-49	0.12692082339217176
50-51	0.03310934392425786
52-53	0.07145941253813241
54-55	0.10577236787435965
56-57	0.03253562248455245
58-59	0.25169219740446813
60-61	0.015005022269218345
62-63	0.015452364820644344
64-65	0.07675631037954625
66-67	0.045832523695625384
68-69	0.06113645153876787
70-71	0.027160502693271915
72-73	0.058686111150952895
74-75	0.014258791488785333
76-77	0.014525596398751393
78-79	0.18401239601220887
80-81	0.0019177599921363817
82-83	0.04635346831546758
84-85	0.03225428828721785
86-87	0.16007527733728125
88-89	0.03184973273427122
90-91	0.033664075021385735
92-93	0.10667011527049237
94-95	0.1213700852943567
96-97	0.07887095663362372
98-99	0.011591899169394947
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62	2.0
63	1.0
64	0.0
65	0.0
66	3.0
67	4.0
68	1.0
69	2.0
70	4.0
71	3.0
72	2.0
73	3.0
74	8.0
75	5.0
76	11.0
77	14.0
78	21.0
79	36.0
80	62.0
81	147.0
82	174.0
83	187.0
84	201.0
85	214.0
86	273.0
87	275.0
88	362.0
89	636.0
90	837.0
91	1209.0
92	1906.0
93	3512.0
94	6891.0
95	13894.0
96	32412.0
97	87958.0
98	336854.0
99	2128403.0
100	4.7233449E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.95975716900941
#Duplication Level	Percentage of total
1	69.2343531740642
2	15.671836825453717
3	7.023770683562278
4	3.3886286895183533
5	1.62528618886171
6	0.9008306301797239
7	0.5327976446027665
8	0.3944646339600842
9	0.26491904818014084
>10	0.9402701888252535
>50	0.01702569195094671
>100	0.0022405965322191846
>500	0.0
>1k	0.0035760043086044482
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	6.01805705984693E-6	0.0	2.0060190199489763E-6	1.4042133139642835E-5	4.4132418438877484E-5	3.41023233391326E-5
2	8.024076079795905E-6	2.0060190199489763E-6	2.0060190199489765E-5	1.604815215959181E-5	6.419260863836724E-5	6.419260863836724E-5
3	1.203611411969386E-5	6.01805705984693E-6	2.2066209219438742E-5	2.6078247259336693E-5	8.224677981790804E-5	8.024076079795906E-5
4	1.203611411969386E-5	1.0030095099744882E-5	2.6078247259336693E-5	3.0090285299234647E-5	8.826483687775497E-5	9.027085589770395E-5
5	1.203611411969386E-5	1.203611411969386E-5	3.209630431918362E-5	3.8114361379030556E-5	1.0030095099744882E-4	1.0431298903734677E-4
6	1.4042133139642835E-5	1.203611411969386E-5	4.2126399418928504E-5	5.416251353862237E-5	1.1434308413709166E-4	1.2437317923683655E-4
7	1.604815215959181E-5	1.8054171179540788E-5	5.015047549872441E-5	7.021066569821418E-5	1.2236716021688757E-4	1.4844540747622426E-4
8	2.407222823938772E-5	2.407222823938772E-5	5.416251353862237E-5	8.024076079795906E-5	1.3440327433658143E-4	1.624875406158671E-4
9	3.209630431918362E-5	2.407222823938772E-5	6.0180570598469295E-5	8.024076079795906E-5	1.4643938845627528E-4	2.0260792101484663E-4
10-11	3.41023233391326E-5	2.9087275789260157E-5	6.920765618823968E-5	9.32798844276274E-5	1.6449355963581608E-4	2.1865607317443842E-4
12-13	4.212639941892851E-5	3.7111351869056066E-5	7.923775128798457E-5	1.0531599854732128E-4	1.8555675934528034E-4	2.6178548210334146E-4
14-15	4.513542794885197E-5	4.012038039897953E-5	8.6258817857806E-5	1.2036114119693859E-4	2.066199590547446E-4	2.858577103427291E-4
16-17	4.613843745882646E-5	4.4132418438877484E-5	9.628891295755088E-5	1.364092933565304E-4	2.4072228239387718E-4	3.2898711927163216E-4
18-19	4.714144696880095E-5	5.416251353862237E-5	1.1133405560716819E-4	1.6048152159591812E-4	2.8385169132278017E-4	3.620864331007902E-4
20-21	5.015047549872441E-5	5.616853255857134E-5	1.2437317923683652E-4	1.8254773081535688E-4	3.1996003368186176E-4	3.9618875643992286E-4
22-23	5.717154206854583E-5	5.717154206854583E-5	1.4042133139642837E-4	1.9358083542507623E-4	3.319961478015556E-4	4.4533622242867276E-4
24-25	7.121367520818866E-5	6.0180570598469295E-5	1.524574455161222E-4	2.066199590547446E-4	3.45035271431224E-4	4.974927169473461E-4
26-27	8.224677981790804E-5	6.218658961841828E-5	1.5947851208594364E-4	2.2968917778415783E-4	3.6308944261076475E-4	5.416251353862237E-4
28-29	9.227687491765292E-5	6.619862765831622E-5	1.70511616695663E-4	2.467403394537241E-4	3.86158661340178E-4	5.616853255857135E-4
30-31	9.829493197749986E-5	7.622872275806111E-5	1.8355074032533136E-4	2.6880654867316285E-4	4.132399181094892E-4	5.927786203949226E-4
32-33	1.0030095099744882E-4	7.923775128798457E-5	2.0160491150487213E-4	2.828486818128057E-4	4.3731214634887684E-4	6.469411339335449E-4
34-35	1.1133405560716819E-4	8.224677981790804E-5	2.1665005415448947E-4	2.95887805442474E-4	4.6038136507829013E-4	6.960885999222949E-4
36-37	1.1935813168696411E-4	8.324978932788252E-5	2.2968917778415783E-4	3.1494498613198927E-4	4.844535933176778E-4	7.542631515008152E-4
38-39	1.2537618874681105E-4	8.826483687775497E-5	2.3470422533403027E-4	3.360081858414536E-4	4.914746598874992E-4	8.234708076890549E-4
40-41	1.2637919825678553E-4	9.327988442762742E-5	2.5777344406344345E-4	3.5105332849107087E-4	5.105318405770145E-4	8.716152641678303E-4
42-43	1.2838521727673448E-4	1.0030095099744882E-4	2.728185867130608E-4	3.6509546163071376E-4	5.275830022465808E-4	9.448349583959679E-4
44-45	1.3139424580665797E-4	1.1033104609719371E-4	2.868607198527036E-4	3.7913759477035654E-4	5.52658239995943E-4	0.0010100305765443096
46-47	1.384153123764794E-4	1.2036114119693859E-4	2.918757674025761E-4	3.9317972790999943E-4	5.707124111754838E-4	0.0010752261946926514
48-49	1.4242735041637732E-4	1.2838521727673448E-4	3.0190586250232096E-4	4.1023088957956564E-4	5.967906584348205E-4	0.0011364097748010951
50-51	1.5646948355602016E-4	1.3440327433658143E-4	3.1193595760206584E-4	4.222670036992596E-4	6.188568676542593E-4	0.0012026084024594115
52-53	1.6549656914579056E-4	1.4042133139642837E-4	3.239720717217597E-4	4.302910797790555E-4	6.379140483437746E-4	0.001279840134727447
54-55	1.6750258816573954E-4	1.5646948355602016E-4	3.340021668215046E-4	4.443332129186983E-4	6.700103526629582E-4	0.001362086914545355
56-57	1.7452365473556096E-4	1.6449355963581608E-4	3.4503527143122395E-4	4.653964126281625E-4	6.990976284522183E-4	0.0014573728179929314
58-59	1.7853569277545892E-4	1.7151462620563747E-4	3.6008041408084127E-4	4.7843553625783093E-4	7.231698566916061E-4	0.0015295895027110946
60-61	1.8756277836522932E-4	1.7753268326548444E-4	3.7412254722048416E-4	4.954866979273971E-4	7.472420849309938E-4	0.0016258784156686454
62-63	1.965898639549997E-4	1.8455374983530583E-4	3.80140604280331E-4	5.085258215570656E-4	7.652962561105345E-4	0.0017362094617658392
64-65	2.066199590547446E-4	1.9257782591510175E-4	3.901706993800759E-4	5.235709642066829E-4	7.983955699396926E-4	0.0018244742986435942
66-67	2.126380161145915E-4	1.9959889248492315E-4	4.012038039897953E-4	5.406221258762491E-4	8.395189598486467E-4	0.0019157481640512727
68-69	2.1765306366446394E-4	2.066199590547446E-4	4.102308895795657E-4	5.50652220975994E-4	8.635911880880344E-4	0.0020180551340686706
70-71	2.2367112072431086E-4	2.1063199709464253E-4	4.1524593712943814E-4	5.56670278035841E-4	8.836513782875242E-4	0.002104313951926476
72-73	2.2868616827418332E-4	2.146440351345405E-4	4.222670036992596E-4	5.727184301954327E-4	9.117356445668099E-4	0.0022096299504737976
74-75	2.2968917778415783E-4	2.146440351345405E-4	4.373121463488769E-4	5.857575538251011E-4	9.327988442762741E-4	0.0022938827493116545
76-77	2.377132538639537E-4	2.1865607317443845E-4	4.4834525095859625E-4	5.95787648924846E-4	9.488469964358658E-4	0.0024012047668789246
78-79	2.4272830141382616E-4	2.2066209219438743E-4	4.533602985084687E-4	6.058177440245909E-4	9.79940291245075E-4	0.0025336020221955573
80-81	2.457373299437496E-4	2.2567713974425987E-4	4.6038136507829013E-4	6.238719152041317E-4	0.0010040125194844627	0.002681044420161807
82-83	2.5677043455346897E-4	2.3269820631408126E-4	4.7342048870795844E-4	6.399200673637235E-4	0.0010401208618435443	0.002812438665968465
84-85	2.6579752014323937E-4	2.3671024435397922E-4	4.8445359331767784E-4	6.529591909933918E-4	0.0010631900805729575	0.0029709141685444343
86-87	2.7482460573300977E-4	2.4172529190385166E-4	4.994987359672952E-4	6.700103526629582E-4	0.0011043134704819117	0.0031193595760206586
88-89	2.8084266279285674E-4	2.5275839651357106E-4	5.145438786169124E-4	6.890675333524734E-4	0.0011464398699008401	0.003274826050066704
>>END_MODULE
