##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674184
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40076452
Total Bases	4 Gbp
Sequences flagged as poor quality	0
Sequence length	62-100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.18271123401842	37.0	35.0	37.0	30.0	37.0
2	34.68896296009437	37.0	35.0	37.0	29.0	37.0
3	34.95558596354787	37.0	35.0	37.0	30.0	37.0
4	34.94094616958607	37.0	35.0	37.0	30.0	37.0
5	34.917170337334255	37.0	35.0	37.0	29.0	37.0
6	34.746374105172784	37.0	35.0	37.0	29.0	37.0
7	34.886366038590445	37.0	35.0	37.0	29.0	37.0
8	34.87465656889986	37.0	35.0	37.0	29.0	37.0
9	34.79683363686985	37.0	35.0	37.0	29.0	37.0
10-11	34.83513604198296	37.0	35.0	37.0	29.0	37.0
12-13	34.81966595995075	37.0	35.0	37.0	29.0	37.0
14-15	34.85454075375735	37.0	35.0	37.0	29.0	37.0
16-17	34.79095043144039	37.0	35.0	37.0	29.0	37.0
18-19	34.80355551434543	37.0	35.0	37.0	29.0	37.0
20-21	34.708403154051666	37.0	35.0	37.0	29.0	37.0
22-23	34.69852228934837	37.0	35.0	37.0	29.0	37.0
24-25	34.7650735399431	37.0	35.0	37.0	29.0	37.0
26-27	34.743311334047235	37.0	35.0	37.0	29.0	37.0
28-29	34.76279595559008	37.0	35.0	37.0	29.0	37.0
30-31	34.77150582092447	37.0	35.0	37.0	29.0	37.0
32-33	34.737518368642014	37.0	35.0	37.0	29.0	37.0
34-35	34.59864381208197	37.0	35.0	37.0	28.5	37.0
36-37	34.7127261415257	37.0	35.0	37.0	29.0	37.0
38-39	34.614856474819675	37.0	35.0	37.0	28.5	37.0
40-41	34.665695618464426	37.0	35.0	37.0	29.0	37.0
42-43	34.70137930373677	37.0	35.0	37.0	29.0	37.0
44-45	34.675991776916774	37.0	35.0	37.0	29.0	37.0
46-47	34.59945964028951	37.0	35.0	37.0	28.5	37.0
48-49	34.61406654710851	37.0	35.0	37.0	28.5	37.0
50-51	34.62788531030641	37.0	35.0	37.0	29.0	37.0
52-53	34.56918269611292	37.0	35.0	37.0	28.5	37.0
54-55	34.53284681987317	37.0	35.0	37.0	28.0	37.0
56-57	34.56484599734527	37.0	35.0	37.0	28.0	37.0
58-59	34.423371285462096	37.0	34.5	37.0	28.0	37.0
60-61	34.50132443111481	37.0	35.0	37.0	28.0	37.0
62-63	34.50672803467761	37.0	35.0	37.0	28.0	37.0
64-65	34.450199636301846	37.0	35.0	37.0	28.0	37.0
66-67	34.488309018803264	37.0	35.0	37.0	28.0	37.0
68-69	34.42038039921658	37.0	34.0	37.0	28.0	37.0
70-71	34.420312767262665	37.0	34.0	37.0	28.0	37.0
72-73	34.428295253426455	37.0	34.0	37.0	28.0	37.0
74-75	34.39012312112247	37.0	34.0	37.0	28.0	37.0
76-77	34.368302492708786	37.0	34.0	37.0	28.0	37.0
78-79	34.265534136094786	37.0	34.0	37.0	27.0	37.0
80-81	34.30059525922485	37.0	34.0	37.0	27.0	37.0
82-83	34.20362626174446	37.0	34.0	37.0	27.0	37.0
84-85	34.152438019709564	37.0	34.0	37.0	27.0	37.0
86-87	34.08348773386604	37.0	34.0	37.0	26.5	37.0
88-89	34.078326185456774	37.0	34.0	37.0	27.0	37.0
90-91	34.02669642941984	37.0	34.0	37.0	26.0	37.0
92-93	33.97034437079893	37.0	34.0	37.0	26.0	37.0
94-95	33.832297626960724	37.0	34.0	37.0	26.0	37.0
96-97	33.89162098132342	37.0	34.0	37.0	26.0	37.0
98-99	33.97171903949829	37.0	34.0	37.0	26.0	37.0
100	34.77558308822917	37.0	34.0	37.0	29.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	367.0
20	14521.0
21	70222.0
22	127867.0
23	186131.0
24	247468.0
25	314284.0
26	403948.0
27	519784.0
28	670756.0
29	864489.0
30	1120009.0
31	1466885.0
32	1976939.0
33	2825653.0
34	4478692.0
35	8718067.0
36	1.5320067E7
37	750303.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	27.79369123254762	27.382847018155566	20.655337285361817	24.168124463934994
2	27.54846651117725	28.847131709286522	24.70241625766894	18.90198552186729
3	19.099222603025996	26.438581147593137	28.560329673208813	25.90186657617205
4	21.819556912698005	27.215169362509233	27.151485899207405	23.813787825585358
5	23.52635942749144	25.911774323549334	26.887219416056524	23.674646832902706
6	23.32719772482956	25.652084139895475	26.794575376835304	24.226142758439657
7	24.30836053129329	26.391276771277777	26.176605101046768	23.123757596382166
8	22.386851975429362	25.414371685631004	27.436509354275035	24.7622669846646
9	23.37811041262307	25.033306309447816	27.19832772388035	24.39025555404876
10-11	24.067896670231328	25.72141953892153	26.09909550374644	24.11158828710071
12-13	24.073684833809445	25.784431468350117	25.867737860686645	24.27414583715379
14-15	23.991874611801205	25.8980074316583	25.917511567238698	24.192606389301798
16-17	24.214975075299698	25.899989435693584	25.845171342044015	24.039864146962707
18-19	24.202852514203034	25.701211141675373	25.775646050348584	24.32029029377301
20-21	24.12390279920085	25.6944362168184	25.9142625543367	24.26739842964405
22-23	24.283997067620174	25.883257138229403	25.72719922579091	24.105546568359514
24-25	24.243979647481893	25.72751485533426	25.73447179921546	24.294033697968388
26-27	24.16032479628934	25.675771223005327	25.900736761326737	24.26316721937859
28-29	24.35048163042535	25.745516282248477	25.715491627517345	24.188510459808832
30-31	24.336042477291908	25.597650694809694	25.669924282596778	24.396382545301616
32-33	24.24878812962204	25.635005002134463	25.76278837777563	24.353418490467867
34-35	24.406703525151695	25.76164574176933	25.62279307943288	24.208857653646096
36-37	24.374970347031567	25.60657787687405	25.61748729835654	24.400964477737848
38-39	24.30019885059983	25.603172318868417	25.72905219414463	24.367576636387124
40-41	24.43802767556668	25.691452106616374	25.603782775662516	24.266737442154426
42-43	24.405288957569564	25.561769495607383	25.583799977695044	24.44914156912801
44-45	24.28734909451876	25.595615754058016	25.73573201145821	24.381303139965013
46-47	24.439627645787073	25.70665118920454	25.601709267983836	24.25201189702455
48-49	24.41296695625756	25.551312003393846	25.595750001936267	24.439971038412327
50-51	24.326310917298247	25.57000467149888	25.698532980119378	24.405151431083496
52-53	24.44776530661484	25.655725695813675	25.610990814997965	24.285518182573522
54-55	24.411970795572064	25.542129627230093	25.576417397590202	24.469482179607642
56-57	24.310383040638957	25.582163141776178	25.70667800400467	24.400775813580196
58-59	24.458334958264878	25.64428275204727	25.63384900301413	24.26353328667372
60-61	24.435688068959664	25.529353643778798	25.577383854846442	24.457574432415097
62-63	24.338056638054685	25.544803360111786	25.695520047690284	24.421619954143246
64-65	24.469125008460534	25.664025506126713	25.572844619061	24.29400486635175
66-67	24.42486997804129	25.54901859828106	25.58196071896753	24.444150704710125
68-69	24.329717178085872	25.55933088758664	25.702256678266554	24.408695256060938
70-71	24.488112459358792	25.653853702503383	25.58101336006542	24.2770204780724
72-73	24.443220953984092	25.523056839871693	25.57800904948794	24.455713156656277
74-75	24.34714649441173	25.542104838710582	25.690553759056606	24.42019490782108
76-77	24.49230429062828	25.649868151808157	25.575581961934535	24.282245595629025
78-79	24.43954058785634	25.519063983091954	25.59691273767562	24.444482691376084
80-81	24.365290793559197	25.5351755880824	25.690290954816696	24.40924266354171
82-83	24.511991576590674	25.62522795915147	25.560585262486185	24.302195201771674
84-85	24.489212377170862	25.499429340212455	25.5531805624977	24.458177720118986
86-87	24.379538222159024	25.539609336267944	25.659561191572834	24.421291250000206
88-89	24.524975468240108	25.62116298114499	25.56067851433186	24.293183036283043
90-91	24.499587023718078	25.47392922469195	25.553379231346895	24.473104520243076
92-93	24.42860329096085	25.489240601417734	25.65721888291676	24.424937224704653
94-95	24.578697762141033	25.576668988054962	25.523840494534678	24.32079275526933
96-97	24.526441619881286	25.478053056277293	25.521813653326276	24.473691670515137
98-99	24.420117730628323	25.44922838064502	25.656297370658503	24.474356518068145
100	25.101886926218498	24.755677318805336	25.214053082704318	24.928382672271844
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	8.0
2	6.0
3	5.0
4	4.5
5	7.0
6	19.0
7	39.0
8	74.5
9	147.5
10	308.0
11	608.0
12	1167.5
13	2079.5
14	3549.5
15	5867.0
16	9214.0
17	13767.0
18	19892.5
19	27899.5
20	38592.5
21	51729.0
22	68122.5
23	88139.0
24	111348.5
25	138797.5
26	170191.0
27	205386.0
28	244020.5
29	286811.0
30	333902.0
31	385190.5
32	441036.0
33	501559.5
34	567556.0
35	638591.5
36	714317.5
37	792794.5
38	873716.5
39	957121.0
40	1039530.0
41	1118573.0
42	1193259.5
43	1259497.5
44	1314751.0
45	1359278.5
46	1390222.5
47	1407053.5
48	1411746.0
49	1404867.0
50	1387061.0
51	1360851.5
52	1330722.5
53	1296678.0
54	1259759.5
55	1221800.0
56	1181908.0
57	1141816.5
58	1097933.5
59	1048178.5
60	991209.5
61	925031.5
62	853432.0
63	774738.0
64	689654.5
65	602218.0
66	515007.0
67	431734.0
68	353047.0
69	280700.0
70	217215.0
71	163522.0
72	120092.0
73	85519.0
74	59073.0
75	39969.5
76	25968.0
77	16434.0
78	10377.5
79	6394.0
80	3957.5
81	2490.0
82	1531.5
83	971.5
84	582.5
85	369.0
86	252.0
87	164.5
88	102.0
89	78.0
90	64.0
91	44.5
92	28.5
93	20.5
94	17.0
95	11.0
96	6.0
97	5.0
98	4.0
99	2.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03995363661433902
2	0.002100984388538187
3	0.0010030828078293956
4	0.059057124118671984
5	0.04598960007737212
6	0.31317892112804796
7	0.031137985967420467
8	0.002842067955516621
9	0.2358941355387448
10-11	0.07738334720848043
12-13	0.1265381476384187
14-15	8.38397570723077E-4
16-17	0.01887642149559547
18-19	0.014650748025299243
20-21	0.17207611092918104
22-23	0.21901015588904926
24-25	0.03931984797456621
26-27	0.039049115425686884
28-29	0.015094899119313257
30-31	6.549981021274039E-4
32-33	0.03132762351317926
34-35	0.27444420479138226
36-37	0.04823905070239252
38-39	0.19859542456502885
40-41	0.09038724286271649
42-43	4.029797847374313E-4
44-45	0.014468596172136198
46-47	0.14860472179523276
48-49	0.12731166920664533
50-51	0.03317035150716436
52-53	0.07057635740808592
54-55	0.10422454562594513
56-57	0.03234193485990227
58-59	0.2505436359486114
60-61	0.014569653022178709
62-63	0.015562755100710451
64-65	0.07542584329981626
66-67	0.04568892931828689
68-69	0.060369622332418556
70-71	0.026787555632741378
72-73	0.058535633282928407
74-75	0.01435631544010185
76-77	0.014291441734490712
78-79	0.18267724740230348
80-81	0.0017878364838014777
82-83	0.045654294547889364
84-85	0.031516443215487674
86-87	0.1598830468762077
88-89	0.031685549986837196
90-91	0.03398709668146195
92-93	0.10615938551533793
94-95	0.12131071279447261
96-97	0.08022105958115622
98-99	0.01121445033380047
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62	1.0
63	1.0
64	1.0
65	1.0
66	0.0
67	0.0
68	1.0
69	2.0
70	0.0
71	0.0
72	3.0
73	1.0
74	4.0
75	2.0
76	5.0
77	7.0
78	7.0
79	24.0
80	40.0
81	108.0
82	116.0
83	137.0
84	171.0
85	164.0
86	225.0
87	234.0
88	294.0
89	530.0
90	682.0
91	1013.0
92	1712.0
93	3043.0
94	6072.0
95	12467.0
96	28771.0
97	78894.0
98	298619.0
99	1841145.0
100	3.7801955E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.22093650825228
#Duplication Level	Percentage of total
1	87.65400615966203
2	9.50269939550645
3	1.905320158499488
4	0.4375366564874167
5	0.15172138010617425
6	0.07645837896446221
7	0.051371600936890895
8	0.04437586224900524
9	0.04241157459144374
>10	0.13409883299663855
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	9.980923460989012E-6	4.990461730494506E-6	2.495230865247253E-6	1.2476154326236264E-5	3.742846297870879E-5	2.9942770382967037E-5
2	1.4971385191483518E-5	9.980923460989012E-6	7.485692595741759E-6	2.7447539517719783E-5	4.740938643969781E-5	4.740938643969781E-5
3	2.495230865247253E-5	1.9961846921978024E-5	9.980923460989012E-6	3.243800124821429E-5	6.238077163118132E-5	5.2399848170192315E-5
4	2.495230865247253E-5	2.7447539517719783E-5	9.980923460989012E-6	3.992369384395605E-5	7.485692595741758E-5	5.988554076593407E-5
5	3.493323211346154E-5	3.742846297870879E-5	1.2476154326236264E-5	4.990461730494506E-5	8.48378494184066E-5	6.986646422692308E-5
6	3.992369384395605E-5	4.24189247092033E-5	1.746661605673077E-5	6.238077163118132E-5	8.733308028365386E-5	7.485692595741758E-5
7	4.24189247092033E-5	4.990461730494506E-5	2.2457077787225275E-5	6.986646422692308E-5	1.0230446547513737E-4	8.98283111489011E-5
8	4.990461730494506E-5	5.7390309900686816E-5	2.2457077787225275E-5	8.733308028365386E-5	1.0729492720563187E-4	1.0230446547513737E-4
9	4.990461730494506E-5	6.986646422692308E-5	2.495230865247253E-5	9.481877287939561E-5	1.272567741276099E-4	1.3474246672335165E-4
10-11	5.6142694468063194E-5	7.735215682266484E-5	2.7447539517719783E-5	1.1228538893612639E-4	1.4971385191483517E-4	1.5345669821270605E-4
12-13	6.238077163118132E-5	9.481877287939561E-5	2.9942770382967037E-5	1.272567741276099E-4	1.7591377599993134E-4	1.8838993032616757E-4
14-15	7.236169509217034E-5	1.0729492720563187E-4	2.9942770382967037E-5	1.372376975885989E-4	1.9213277662403849E-4	2.1458985441126375E-4
16-17	8.858069571627747E-5	1.185234660992445E-4	3.493323211346154E-5	1.659328525389423E-4	2.108470081133929E-4	2.37046932198489E-4
18-19	9.606638831201924E-5	1.3099962042548076E-4	4.24189247092033E-5	1.9088516119141486E-4	2.1958031614175826E-4	2.857039340708105E-4
20-21	1.0604731177300824E-4	1.3973292845384616E-4	4.491415557445055E-5	1.9587562292190934E-4	2.3455170133324178E-4	3.1065624272328296E-4
22-23	1.1602823523399726E-4	1.5220908278008242E-4	4.8657001872321436E-5	2.108470081133929E-4	2.495230865247253E-4	3.518275519998627E-4
24-25	1.2975200499285716E-4	1.7217092970206045E-4	6.362838706380495E-5	2.17085085276511E-4	2.5700877912046707E-4	3.9299886127644234E-4
26-27	1.4347577475171705E-4	1.8215185316304946E-4	7.360931052479395E-5	2.2706600873750003E-4	2.6823731801407964E-4	4.279320933899039E-4
28-29	1.5345669821270605E-4	1.8963754575879123E-4	8.359023398578297E-5	2.3829454763111266E-4	2.80713472340316E-4	4.5787486377287087E-4
30-31	1.5719954451057694E-4	1.9712323835453297E-4	8.858069571627747E-5	2.5201831738997255E-4	2.9568485753179947E-4	5.015414039146978E-4
32-33	1.6343762167369505E-4	2.0460893095027474E-4	8.98283111489011E-5	2.6574208714883244E-4	3.218847816168956E-4	5.37722251460783E-4
34-35	1.6718046797156594E-4	2.120946235460165E-4	9.980923460989012E-5	2.7821824147506867E-4	3.3685616680837914E-4	5.801411761699863E-4
36-37	1.796566222978022E-4	2.3704693219848904E-4	1.0604731177300824E-4	2.944372420991758E-4	3.568180137303572E-4	6.263029471770605E-4
38-39	1.9088516119141483E-4	2.532659328225962E-4	1.1103777350350276E-4	3.156467044537775E-4	3.680465526239698E-4	6.699694873188874E-4
40-41	1.9712323835453297E-4	2.607516254183379E-4	1.1727585066662089E-4	3.318657050778846E-4	3.8551316868070057E-4	7.111407965954671E-4
42-43	2.1833270070913463E-4	2.7821824147506867E-4	1.3099962042548076E-4	3.4683709026936817E-4	4.017321693048077E-4	7.622930293330358E-4
44-45	2.2831362417012363E-4	2.881991649360577E-4	1.372376975885989E-4	3.60560860028228E-4	4.1545593906366766E-4	8.171881083684754E-4
46-47	2.3829454763111266E-4	2.9693247296442313E-4	1.4222815931909342E-4	3.7054178348921703E-4	4.254368625246566E-4	8.708355719712912E-4
48-49	2.457802402268544E-4	3.069133964254121E-4	1.5345669821270605E-4	3.8052270695020606E-4	4.329225551203984E-4	9.194925738436127E-4
50-51	2.532659328225962E-4	3.20637166184272E-4	1.646852371063187E-4	3.917512458438187E-4	4.528844020423764E-4	9.706448065811815E-4
52-53	2.632468562835852E-4	3.3560855137575553E-4	1.7217092970206045E-4	4.1296070819842035E-4	4.715986335317308E-4	0.0010242922701839974
54-55	2.744753951771978E-4	3.5432278286510994E-4	1.796566222978022E-4	4.291797088225275E-4	4.8657001872321436E-4	0.0010929111189782968
56-57	2.919420112339286E-4	3.692941680565934E-4	1.8589469946092034E-4	4.478939403118819E-4	5.002937884820742E-4	0.0011677680449357145
58-59	2.9942770382967033E-4	3.7428462978708794E-4	1.9213277662403849E-4	4.703510180991072E-4	5.115223273756868E-4	0.001252605894354121
60-61	3.1065624272328296E-4	3.8551316868070057E-4	1.9961846921978023E-4	4.953033267515797E-4	5.215032508366758E-4	0.0013137390505526788
62-63	3.268752433473901E-4	3.929988612764423E-4	2.0585654638289837E-4	5.077794810778159E-4	5.551888675175137E-4	0.0013811102839143545
64-65	3.30618089645261E-4	4.0672263103530223E-4	2.2457077787225278E-4	5.177604045388049E-4	5.838840224678572E-4	0.0014447386709781594
66-67	3.4059901310625005E-4	4.2668447795728024E-4	2.2956123960274726E-4	5.314841742976649E-4	6.125791774182006E-4	0.001509614673474588
68-69	3.4808470570199177E-4	4.416558631487638E-4	2.357993167658654E-4	5.427127131912775E-4	6.325410243401787E-4	0.0015957001383256181
70-71	3.5432278286510994E-4	4.603700946381182E-4	2.457802402268544E-4	5.539412520848901E-4	6.587409484252747E-4	0.0017054902963964972
72-73	3.7802747608495885E-4	4.7534147982960167E-4	2.5201831738997255E-4	5.551888675175139E-4	6.74959949049382E-4	0.0017815948377865386
74-75	3.8676078411332423E-4	4.890652495884616E-4	2.6574208714883244E-4	5.689126372763737E-4	7.036551039997254E-4	0.0018726707643680633
76-77	3.905036304111951E-4	4.977985576168269E-4	2.7572301060982147E-4	5.888744841983517E-4	7.273597972195742E-4	0.0019662419218148355
78-79	4.029797847374313E-4	5.127699428083105E-4	2.832087032055632E-4	5.976077922267171E-4	7.635406447656594E-4	0.002053575002098489
80-81	4.1670355449629126E-4	5.27741327999794E-4	2.9568485753179947E-4	6.138267928508242E-4	7.909881842833792E-4	0.0021920603151197116
82-83	4.291797088225275E-4	5.464555594891483E-4	3.069133964254121E-4	6.250553317444368E-4	8.221785700989698E-4	0.0023055933194884616
84-85	4.3666540141826927E-4	5.551888675175139E-4	3.2188478161689565E-4	6.387791015032968E-4	8.48378494184066E-4	0.002449069094240179
86-87	4.416558631487638E-4	5.739030990068682E-4	3.343609359431319E-4	6.549981021274039E-4	8.845593417301512E-4	0.0025825639455309066
88-89	4.5038917117712915E-4	5.801411761699863E-4	3.4558947483674456E-4	6.687218718862639E-4	9.169973429783655E-4	0.002726039720282624
>>END_MODULE
