##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674185
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	47926700
Total Bases	4.7 Gbp
Sequences flagged as poor quality	0
Sequence length	61-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.452790970377684	37.0	36.0	37.0	31.0	37.0
2	34.995284423922364	37.0	35.0	37.0	30.0	37.0
3	35.314252639968956	37.0	36.0	37.0	31.0	37.0
4	35.27000377660052	37.0	36.0	37.0	31.0	37.0
5	35.25224609664342	37.0	36.0	37.0	31.0	37.0
6	35.08694170472826	37.0	36.0	37.0	30.0	37.0
7	35.24180258603242	37.0	36.0	37.0	31.0	37.0
8	35.234413489766666	37.0	36.0	37.0	31.0	37.0
9	35.15176544598314	37.0	36.0	37.0	30.0	37.0
10-11	35.20748696446866	37.0	36.0	37.0	30.5	37.0
12-13	35.19523703906174	37.0	36.0	37.0	30.5	37.0
14-15	35.23711799477118	37.0	36.0	37.0	31.0	37.0
16-17	35.191459030143946	37.0	36.0	37.0	30.0	37.0
18-19	35.20950755007125	37.0	36.0	37.0	31.0	37.0
20-21	35.1199098727849	37.0	36.0	37.0	30.5	37.0
22-23	35.11787025812335	37.0	36.0	37.0	30.0	37.0
24-25	35.19192686957375	37.0	36.0	37.0	30.5	37.0
26-27	35.1747710879322	37.0	36.0	37.0	30.0	37.0
28-29	35.20231621413534	37.0	36.0	37.0	31.0	37.0
30-31	35.21500430866302	37.0	36.0	37.0	31.0	37.0
32-33	35.186393993327314	37.0	36.0	37.0	30.5	37.0
34-35	35.05819903102029	37.0	36.0	37.0	30.0	37.0
36-37	35.17655545864831	37.0	36.0	37.0	30.5	37.0
38-39	35.08370904944425	37.0	36.0	37.0	30.0	37.0
40-41	35.143317962638775	37.0	36.0	37.0	30.0	37.0
42-43	35.187984974972196	37.0	36.0	37.0	30.5	37.0
44-45	35.1661418687287	37.0	36.0	37.0	30.0	37.0
46-47	35.102191231609936	37.0	36.0	37.0	30.0	37.0
48-49	35.122126935507765	37.0	36.0	37.0	30.0	37.0
50-51	35.1428865329764	37.0	36.0	37.0	30.0	37.0
52-53	35.095446629957834	37.0	35.5	37.0	30.0	37.0
54-55	35.06530442321295	37.0	35.0	37.0	30.0	37.0
56-57	35.105685494724234	37.0	35.5	37.0	30.0	37.0
58-59	34.977334085175904	37.0	35.0	37.0	30.0	37.0
60-61	35.06462180788579	37.0	35.0	37.0	30.0	37.0
62-63	35.07364797941429	37.0	35.0	37.0	30.0	37.0
64-65	35.03184483948175	37.0	35.0	37.0	30.0	37.0
66-67	35.07126859944612	37.0	35.0	37.0	30.0	37.0
68-69	35.0164263258161	37.0	35.0	37.0	30.0	37.0
70-71	35.03041440856657	37.0	35.0	37.0	30.0	37.0
72-73	35.04115518041101	37.0	35.0	37.0	30.0	37.0
74-75	35.0140319218535	37.0	35.0	37.0	30.0	37.0
76-77	35.004456546946315	37.0	35.0	37.0	30.0	37.0
78-79	34.90841831841874	37.0	35.0	37.0	30.0	37.0
80-81	34.95761206153033	37.0	35.0	37.0	30.0	37.0
82-83	34.879880745035095	37.0	35.0	37.0	29.0	37.0
84-85	34.84302212995433	37.0	35.0	37.0	29.0	37.0
86-87	34.782644122005145	37.0	35.0	37.0	29.0	37.0
88-89	34.79725621503033	37.0	35.0	37.0	29.0	37.0
90-91	34.76180781991186	37.0	35.0	37.0	29.0	37.0
92-93	34.711109215205155	37.0	35.0	37.0	29.0	37.0
94-95	34.59681567771325	37.0	35.0	37.0	28.5	37.0
96-97	34.659121489811604	37.0	35.0	37.0	29.0	37.5
98-99	34.706323526006244	37.0	35.0	37.0	29.0	37.5
100	35.220019446559206	37.0	35.0	37.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	126.0
20	7261.0
21	39228.0
22	76231.0
23	119532.0
24	169769.0
25	228741.0
26	310897.0
27	420865.0
28	564602.0
29	752933.0
30	1001062.0
31	1338189.0
32	1834631.0
33	2679580.0
34	4423357.0
35	9750047.0
36	2.2932466E7
37	1277183.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	27.878563366714985	32.08067829890574	17.639027005866772	22.401731328512504
2	21.223947544598495	31.024867364907717	30.58657193384797	17.16461315664582
3	16.573699867316474	28.347431783995642	31.894797549642607	23.18407079904528
4	19.106723095542154	28.38283261307961	31.71513695287413	20.795307338504106
5	20.782022030389623	28.68509985581764	29.996744107721124	20.536134006071613
6	19.977137577288985	28.66641756083896	30.434621713641857	20.921823148230207
7	21.402767352095353	29.472031614715892	28.629805998994197	20.49539503419456
8	19.611477668230854	28.352746047141324	30.163325364625702	21.872450920002116
9	19.992158092552955	27.56020531684002	31.08990863803444	21.357727952572585
10-11	21.36856937602546	28.832052032371468	28.59827788690407	21.201100704698998
12-13	21.13824395964367	28.983723076446104	28.652038463703168	21.225994500207058
14-15	20.99251952188279	28.946200426586405	28.67826705896221	21.3830129925686
16-17	21.26323313803914	28.939262142576965	28.58584038434447	21.211664335039426
18-19	21.085272864346972	28.697312000636398	29.00180003525487	21.215615099761752
20-21	20.851186928716473	28.71561461591258	29.025325891907823	21.40787256346313
22-23	21.350483087011703	29.02176617066925	28.479774906764515	21.14797583555453
24-25	21.129180413306788	28.92701229186333	28.8601907627588	21.08361653207108
26-27	20.792801641192646	28.807609245595632	29.14396060105677	21.255628512154953
28-29	21.31803586063299	28.870158713543493	28.624987082445802	21.186818343377723
30-31	21.21719509394077	28.839114201184618	28.78933680098605	21.154353903888566
32-33	20.897984507501565	28.79958428473315	28.977555652212654	21.324875555552634
34-35	21.396884826795436	28.92831566439015	28.480756070454156	21.194043438360254
36-37	21.266603634860154	28.797815166434624	28.76666701772091	21.168914180984313
38-39	20.952739042241568	28.791259586416036	28.937422582748994	21.3185787885934
40-41	21.392316930889873	28.873685952813343	28.517149791185208	21.216847325111573
42-43	21.25481119436427	28.813147020576857	28.75180622899114	21.18023555606773
44-45	20.91850385321431	28.774393226922186	28.961004747889916	21.346098171973587
46-47	21.39716239986849	28.89735375742766	28.52114678124675	21.18433706145711
48-49	21.271759127674507	28.768423718980408	28.782409762624074	21.177407390721015
50-51	20.96822605080184	28.72254777682667	28.947458418331124	21.36176775404037
52-53	21.371140024270083	28.839827915806502	28.568837258257812	21.220194801665603
54-55	21.277989059576953	28.757451432687773	28.75556008948779	21.20899941824748
56-57	20.957136425440684	28.715236547997847	28.968381374329	21.359245652232467
58-59	21.412243679621778	28.815133682669458	28.59283927184524	21.179783365863525
60-61	21.28668015680818	28.76163843991659	28.755646090841047	21.196035312434173
62-63	20.94724613621326	28.72589832606074	28.962508166353924	21.364347371372077
64-65	21.422848143760838	28.85077722207728	28.506497012212655	21.21987762194923
66-67	21.269529925353538	28.767651045829755	28.780389831257487	21.182429197559223
68-69	20.9455903869148	28.72974267346092	28.99526566569265	21.329401273931627
70-71	21.445589156727326	28.793896157798493	28.564332329281385	21.196182356192793
72-73	21.274018093503262	28.767394365295925	28.789705024751335	21.16888251644948
74-75	20.97449419330482	28.73305318686184	28.96658706737246	21.325865552460886
76-77	21.44015784058764	28.840814569936995	28.523777168618015	21.195250420857352
78-79	21.268519319643648	28.769285502244845	28.771036209368518	21.19115896874299
80-81	20.995194862724222	28.705399535406716	28.986783525112468	21.312622076756586
82-83	21.43977219228044	28.82874151127147	28.542520613876942	21.188965682571148
84-85	21.314773150788866	28.71587560429226	28.797290795321416	21.17206044959745
86-87	21.007577134923803	28.70915453235863	28.951738462610578	21.33152987010699
88-89	21.457910371138	28.80871202813663	28.544697102512874	21.188680498212502
90-91	21.336167444736475	28.719485256193206	28.772566400534217	21.171780898536106
92-93	21.022793613977463	28.679692459325945	28.9764214715297	21.32109245516689
94-95	21.478251251776403	28.777532397282467	28.549559733316677	21.194656617624453
96-97	21.346909283047893	28.74838298283697	28.746236066499915	21.158471667615228
98-99	21.036759878227087	28.70345735066588	28.940835733354458	21.31894703775258
100	21.997260777759923	28.378235185900476	28.28743349782905	21.33707053851055
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	7.0
2	2.5
3	2.0
4	2.5
5	6.5
6	12.5
7	23.0
8	60.0
9	139.0
10	263.0
11	487.0
12	905.5
13	1823.5
14	3462.0
15	6313.0
16	10556.0
17	16736.0
18	26279.5
19	39944.5
20	58474.5
21	83350.0
22	114317.0
23	151056.5
24	198053.0
25	257596.5
26	327848.5
27	411524.5
28	509593.5
29	618688.5
30	745525.0
31	889819.0
32	1050115.0
33	1223434.0
34	1405340.5
35	1594608.0
36	1777083.5
37	1945642.5
38	2093507.5
39	2209096.5
40	2287827.0
41	2327073.5
42	2316126.5
43	2257438.5
44	2166257.0
45	2051330.5
46	1912517.0
47	1749418.5
48	1583213.0
49	1422427.5
50	1264872.5
51	1116807.5
52	980361.5
53	856018.5
54	748804.5
55	660946.0
56	587926.5
57	526532.5
58	474533.0
59	430612.0
60	390163.5
61	351748.0
62	314175.0
63	276326.5
64	237625.5
65	200311.0
66	165502.5
67	132760.5
68	104327.5
69	79811.5
70	58888.5
71	42433.0
72	29934.5
73	20542.5
74	14041.5
75	9371.5
76	6011.0
77	3768.5
78	2366.5
79	1493.5
80	938.0
81	581.5
82	366.0
83	231.0
84	162.0
85	102.5
86	63.5
87	43.5
88	31.0
89	23.0
90	17.5
91	12.0
92	8.5
93	10.5
94	8.5
95	5.0
96	7.5
97	6.0
98	2.5
99	3.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04056820102364653
2	0.002159547809467374
3	0.0013145073622844886
4	0.06001665042658894
5	0.047533003524131645
6	0.3139919919376882
7	0.0309535186023657
8	0.0027562924215520784
9	0.2385997784116161
10-11	0.07875151011857691
12-13	0.12706487198158856
14-15	8.732084620889818E-4
16-17	0.01911982256237129
18-19	0.014778818487398464
20-21	0.1720585811249262
22-23	0.22002245095113998
24-25	0.04013629146175305
26-27	0.03883638973682728
28-29	0.015465283443258144
30-31	6.927245147276988E-4
32-33	0.03142402877727864
34-35	0.2774090433933486
36-37	0.04876509336132052
38-39	0.20071171184329403
40-41	0.09071874341442245
42-43	3.9852524793069415E-4
44-45	0.014808029762115898
46-47	0.14868747483135705
48-49	0.12754164171536952
50-51	0.03316418614258858
52-53	0.06920359632522163
54-55	0.10572812232012636
56-57	0.032525711138050396
58-59	0.25002764638500047
60-61	0.015152305499857072
62-63	0.01568332523468104
64-65	0.07614127804236877
66-67	0.04551742936754914
68-69	0.06081683768113822
70-71	0.027131018899332556
72-73	0.058546711400684404
74-75	0.014335437853367865
76-77	0.014472107199004297
78-79	0.18538012230958847
80-81	0.0017297276905762955
82-83	0.046247973769125775
84-85	0.032501033334221156
86-87	0.15952448397809016
88-89	0.031553151949031535
90-91	0.03316875404880606
92-93	0.10723278107515548
94-95	0.12123892090536205
96-97	0.07906827645834584
98-99	0.011255413622935346
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
61	1.0
62	1.0
63	0.0
64	1.0
65	1.0
66	0.0
67	0.0
68	3.0
69	0.0
70	1.0
71	3.0
72	1.0
73	3.0
74	2.0
75	2.0
76	9.0
77	7.0
78	11.0
79	14.0
80	42.0
81	118.0
82	112.0
83	124.0
84	151.0
85	138.0
86	166.0
87	186.0
88	243.0
89	440.0
90	596.0
91	831.0
92	1295.0
93	2227.0
94	4381.0
95	8506.0
96	19744.0
97	55015.0
98	217247.0
99	1542146.0
100	4.6072932E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.54106605314672
#Duplication Level	Percentage of total
1	62.29634339775985
2	13.574818894994555
3	5.512100335280631
4	2.3692236388409538
5	1.2801451479441313
6	0.7750858387603884
7	0.5979221303047341
8	0.48144172146782827
9	0.43184804739272314
>10	8.312966576030625
>50	3.2932654627242464
>100	1.0731486729973188
>500	0.001690135501915681
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.0865196226737915E-6	2.0865196226737915E-6	2.0865196226737915E-6	8.346078490695166E-6	2.2951715849411707E-5	3.547083358545445E-5
2	2.0865196226737915E-6	2.0865196226737915E-6	6.2595588680213745E-6	1.0432598113368957E-5	5.633602981219237E-5	5.424951018951858E-5
3	2.0865196226737915E-6	4.173039245347583E-6	1.460563735871654E-5	1.0432598113368957E-4	6.676862792556133E-5	6.468210830288753E-5
4	4.173039245347583E-6	6.2595588680213745E-6	1.460563735871654E-5	1.1058554000171095E-4	8.972034377497304E-5	7.09416671709089E-5
5	4.173039245347583E-6	8.346078490695166E-6	1.6692156981390332E-5	1.1893161849240611E-4	1.1058554000171095E-4	8.763382415229924E-5
6	4.173039245347583E-6	8.346078490695166E-6	2.0865196226737913E-5	1.2519117736042748E-4	1.3562377547379645E-4	9.59799026429944E-5
7	6.2595588680213745E-6	1.2519117736042749E-5	2.0865196226737913E-5	1.3145073622844886E-4	1.481428932098392E-4	1.0015294188834199E-4
8	8.346078490695166E-6	1.8778676604064123E-5	2.2951715849411707E-5	1.3145073622844886E-4	1.731811286819247E-4	1.1267205962438474E-4
9	8.346078490695166E-6	2.0865196226737913E-5	2.5038235472085498E-5	1.3562377547379645E-4	1.919598052859888E-4	1.439698539644916E-4
10-11	1.2519117736042749E-5	2.190845603807481E-5	3.0254534528769973E-5	1.439698539644916E-4	2.3577671736213843E-4	1.6483505019122954E-4
12-13	1.8778676604064123E-5	2.5038235472085498E-5	3.3384313962780664E-5	1.5335919226652366E-4	2.90026227551657E-4	1.8152720717261984E-4
14-15	2.0865196226737913E-5	3.442757377411756E-5	3.7557353208128245E-5	1.6170527075721883E-4	3.4323247792983867E-4	1.919598052859888E-4
16-17	2.2951715849411707E-5	3.7557353208128245E-5	4.486017188748652E-5	1.7526764830459848E-4	3.9226568906267274E-4	2.0760870245604225E-4
18-19	2.712475509475929E-5	3.964387283080204E-5	5.1119730755507886E-5	1.909165454746519E-4	4.632073562335817E-4	2.430795360414967E-4
20-21	3.3384313962780664E-5	4.1730392453475827E-5	5.216299056684478E-5	2.0656544264470537E-4	5.10154047743742E-4	2.5768517340021325E-4
22-23	3.442757377411756E-5	4.2773652264812724E-5	5.8422549434866165E-5	2.243008594374326E-4	5.414518420838489E-4	2.7855036962695114E-4
24-25	3.547083358545445E-5	4.381691207614962E-5	6.050906905753995E-5	2.3786323698481222E-4	5.790091952919772E-4	3.2445380132577455E-4
26-27	3.547083358545445E-5	4.79899513214972E-5	6.676862792556133E-5	2.524688743435288E-4	6.322154456701588E-4	3.651409339679135E-4
28-29	3.7557353208128245E-5	4.79899513214972E-5	7.40714466049196E-5	2.629014724568977E-4	6.781188773689823E-4	3.8287635076064073E-4
30-31	3.860061301946514E-5	4.90332111328341E-5	8.137426528427786E-5	2.7750710981561426E-4	7.407144660491959E-4	3.964387283080204E-4
32-33	4.5903431698823415E-5	5.1119730755507886E-5	8.659056434096234E-5	2.910694873629939E-4	8.033100547294097E-4	4.100011058554E-4
34-35	4.79899513214972E-5	5.5292770000855474E-5	9.389338302032061E-5	3.150644630237425E-4	8.471269668055594E-4	4.266932628367903E-4
36-37	5.1119730755507886E-5	6.259558868021374E-5	9.493664283165751E-5	3.2445380132577455E-4	8.930303985043828E-4	4.392123805728331E-4
38-39	5.633602981219237E-5	6.363884849155064E-5	1.0119620169967889E-4	3.609678947225659E-4	9.577125068072703E-4	4.611208366109079E-4
40-41	5.842254943486616E-5	6.468210830288753E-5	1.0641250075636336E-4	3.682707134019242E-4	0.001038043512280211	4.882455917056672E-4
42-43	6.468210830288753E-5	6.676862792556133E-5	1.1267205962438474E-4	3.7766005170395626E-4	0.0011027256205830987	5.11197307555079E-4
44-45	6.781188773689823E-5	6.9898407359572E-5	1.1893161849240611E-4	3.870493900059883E-4	0.0011548886111499436	5.404085822725119E-4
46-47	7.19849269822458E-5	7.615796622759339E-5	1.2936421660577507E-4	4.0165502736470485E-4	0.0012373061362455583	5.675333373672713E-4
48-49	7.511470641625649E-5	7.824448585026718E-5	1.3562377547379645E-4	4.079145862327262E-4	0.0012894691268124031	5.957013522733675E-4
50-51	8.033100547294098E-5	8.033100547294098E-5	1.4084007453048092E-4	4.152174049120845E-4	0.0013520647154926168	6.228261073681267E-4
52-53	8.450404471828855E-5	8.763382415229924E-5	1.481428932098392E-4	4.2565000302545344E-4	0.001439698539644916	6.509941222742229E-4
54-55	8.763382415229924E-5	8.972034377497304E-5	1.5335919226652366E-4	4.392123805728331E-4	0.0015388082217219212	6.69772798878287E-4
56-57	9.180686339764683E-5	9.076360358630993E-5	1.5753223151187125E-4	4.4860171887486515E-4	0.0016045335898361456	7.083734118977522E-4
58-59	9.285012320898371E-5	9.389338302032061E-5	1.6379179037989263E-4	4.569477973655603E-4	0.0016650426588936855	7.480172847285542E-4
60-61	9.493664283165751E-5	9.493664283165751E-5	1.742243884932616E-4	4.684236552902662E-4	0.0017255517279512254	7.824448585026717E-4
62-63	9.70231624543313E-5	1.0015294188834199E-4	1.7944068754994607E-4	4.7781299359229827E-4	0.0017954501353107974	8.210454715221369E-4
64-65	1.0015294188834199E-4	1.0015294188834199E-4	1.8465698660663054E-4	4.882455917056672E-4	0.0018663918024817063	8.617326041642759E-4
66-67	1.0223946151101579E-4	1.0849902037903715E-4	1.9300306509732572E-4	4.99721449630373E-4	0.0019404632490866262	9.055495162404255E-4
68-69	1.0536924094502646E-4	1.1580183905839542E-4	2.013491435880209E-4	5.10154047743742E-4	0.0020166212153142193	9.431068694485537E-4
70-71	1.0954228019037405E-4	1.1788835868106921E-4	2.0552218283336846E-4	5.247596851024586E-4	0.0020990387404098343	9.723181441659868E-4
72-73	1.1267205962438474E-4	1.2101813811507989E-4	2.0865196226737913E-4	5.445816215178596E-4	0.00218458604493946	0.0010244811347328315
74-75	1.2310465773775369E-4	1.2310465773775369E-4	2.1178174170138984E-4	5.59187258876576E-4	0.0022471816336196732	0.0010547356692616017
76-77	1.3040747641711195E-4	1.241479175490906E-4	2.169980407580743E-4	5.779659354806402E-4	0.002319166560601919	0.0010849902037903714
78-79	1.3145073622844886E-4	1.2519117736042748E-4	2.211710800034219E-4	6.176098083114423E-4	0.002371329551168764	0.0011225475569984998
80-81	1.3145073622844886E-4	1.2519117736042748E-4	2.2534411924876949E-4	6.363884849155063E-4	0.002442271218339673	0.0011611481700179649
82-83	1.3353725585112266E-4	1.2519117736042748E-4	2.2951715849411707E-4	6.770756175576453E-4	0.0024881746500384962	0.001200792042848767
84-85	1.3666703528513336E-4	1.2936421660577507E-4	2.3577671736213843E-4	7.00027333407057E-4	0.002562246096643416	0.0012466954745475904
86-87	1.3875355490780713E-4	1.3771029509647024E-4	2.368199771734753E-4	7.115031913317629E-4	0.0026164956068329345	0.00129051238662374
88-89	1.481428932098392E-4	1.3979681471914404E-4	2.420362762301598E-4	7.271520885018163E-4	0.0026738748964564634	0.0013218101809638469
>>END_MODULE
