##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674189
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	42336336
Total Bases	4.2 Gbp
Sequences flagged as poor quality	0
Sequence length	69-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.854847453024746	37.0	35.0	37.0	29.0	37.0
2	34.34784061143128	37.0	34.0	37.0	28.0	37.0
3	34.623706524815944	37.0	35.0	37.0	29.0	37.0
4	34.611098867885026	37.0	35.0	37.0	29.0	37.0
5	34.61037256507034	37.0	35.0	37.0	28.0	37.0
6	34.415307763052525	37.0	34.0	37.0	28.0	37.0
7	34.571824897648206	37.0	35.0	37.0	28.0	37.0
8	34.56646515182608	37.0	35.0	37.0	28.0	37.0
9	34.508719200452305	37.0	35.0	37.0	28.0	37.0
10-11	34.552330579575894	37.0	34.5	37.0	28.0	37.0
12-13	34.53855400949199	37.0	35.0	37.0	28.0	37.0
14-15	34.57979078539059	37.0	35.0	37.0	28.0	37.0
16-17	34.51553091415374	37.0	34.0	37.0	28.0	37.0
18-19	34.534861283224885	37.0	34.5	37.0	28.0	37.0
20-21	34.44752426142877	37.0	34.5	37.0	28.0	37.0
22-23	34.43594151605373	37.0	34.0	37.0	28.0	37.0
24-25	34.505558865557	37.0	34.0	37.0	28.0	37.0
26-27	34.49089827943543	37.0	34.0	37.0	28.0	37.0
28-29	34.518617163752666	37.0	34.0	37.0	28.0	37.0
30-31	34.528049014444704	37.0	34.0	37.0	28.0	37.0
32-33	34.50070620187822	37.0	34.0	37.0	28.0	37.0
34-35	34.36766105597802	37.0	34.0	37.0	28.0	37.0
36-37	34.48276856079373	37.0	34.0	37.0	28.0	37.0
38-39	34.39143170065544	37.0	34.0	37.0	28.0	37.0
40-41	34.44949506258643	37.0	34.0	37.0	28.0	37.0
42-43	34.485658454241296	37.0	34.0	37.0	28.0	37.0
44-45	34.46891497648734	37.0	34.0	37.0	28.0	37.0
46-47	34.397587677403166	37.0	34.0	37.0	28.0	37.0
48-49	34.411656419676945	37.0	34.0	37.0	28.0	37.0
50-51	34.43923871919384	37.0	34.0	37.0	28.0	37.0
52-53	34.379593016740984	37.0	34.0	37.0	27.5	37.0
54-55	34.347250232046534	37.0	34.0	37.0	27.5	37.0
56-57	34.386628923202046	37.0	34.0	37.0	28.0	37.0
58-59	34.24597579724424	37.0	34.0	37.0	27.5	37.0
60-61	34.32445734085255	37.0	34.0	37.0	27.0	37.0
62-63	34.346083267574215	37.0	34.0	37.0	27.5	37.0
64-65	34.29395975598833	37.0	34.0	37.0	27.0	37.0
66-67	34.33950063604937	37.0	34.0	37.0	28.0	37.0
68-69	34.277632551858055	37.0	34.0	37.0	27.0	37.0
70-71	34.28607966449858	37.0	34.0	37.0	27.0	37.0
72-73	34.301534547351395	37.0	34.0	37.0	27.0	37.0
74-75	34.27598956340056	37.0	34.0	37.0	27.0	37.0
76-77	34.26029167726499	37.0	34.0	37.0	27.0	37.0
78-79	34.161621209974996	37.0	34.0	37.0	27.0	37.0
80-81	34.20625099808483	37.0	34.0	37.0	27.0	37.0
82-83	34.11810685687368	37.0	34.0	37.0	27.0	37.0
84-85	34.070966079570255	37.0	34.0	37.0	27.0	37.0
86-87	34.01735306264452	37.0	34.0	37.0	26.5	37.0
88-89	34.02120287956933	37.0	34.0	37.0	26.0	37.0
90-91	33.974474148156176	37.0	34.0	37.0	26.0	37.0
92-93	33.93670087064217	37.0	34.0	37.0	26.0	37.0
94-95	33.80220265248556	37.0	33.0	37.0	26.0	37.0
96-97	33.873526615293116	37.0	33.5	37.0	26.0	37.0
98-99	33.9542228121412	37.0	33.5	37.0	26.0	37.0
100	34.69622503819665	37.0	34.0	37.0	29.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	249.0
20	14850.0
21	79848.0
22	149315.0
23	218361.0
24	288778.0
25	366737.0
26	467468.0
27	603478.0
28	776803.0
29	1002200.0
30	1297975.0
31	1694122.0
32	2279034.0
33	3238596.0
34	5079910.0
35	9566809.0
36	1.4708362E7
37	503441.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.567077101355032	36.975329967182866	17.73456640176033	23.72302652970177
2	23.65517993009752	29.940096210390738	31.33592069379419	15.06880316571755
3	16.186898576412844	29.204641627777395	32.860212854258116	21.74824694155165
4	17.83591984954158	30.017668597816975	30.802748698548644	21.343662854092806
5	20.631363788205416	29.10216742409677	29.682178528912505	20.58429025878531
6	20.310994771220923	28.096893831557136	30.773640129442935	20.818471267779003
7	20.923965666487003	29.610771833015637	29.186087458990574	20.27917504150679
8	19.005088585261994	27.86414153384519	31.602163365634784	21.52860651525803
9	20.149403810542026	27.08653761875478	31.43405004677718	21.33000852392602
10-11	21.09115515576355	28.199069497240973	29.398463641241268	21.31131170575421
12-13	21.17072299589022	28.485606975016935	28.86900732888384	21.474662700209006
14-15	21.17775117051737	28.56198203041479	28.899299130432315	21.36096766863552
16-17	21.307061977544507	28.606678370182376	28.814405792492547	21.27185385978057
18-19	21.274735324938984	28.37649677945829	28.792978580520845	21.55578931508188
20-21	21.329871970090135	28.32132459571814	28.85316014544994	21.49564328874179
22-23	21.471788214114486	28.56651581497463	28.565345241478486	21.3963507294324
24-25	21.518050816152883	28.36558461193729	28.462408981040628	21.6539555908692
26-27	21.512965657399942	28.301960712545025	28.63517039683922	21.54990323321581
28-29	21.624923342080812	28.433077345680353	28.44518693157398	21.496812380664856
30-31	21.603795592107115	28.275733809497982	28.40698599793991	21.713484600454986
32-33	21.577517537753856	28.277330962749776	28.526826549608074	21.618324949888294
34-35	21.682435471787063	28.431283057078442	28.338149114353566	21.548132356780926
36-37	21.67415184557445	28.263742676577188	28.30382608759972	21.758279390248642
38-39	21.651174225960975	28.226404361790745	28.46747221741246	21.654949194835822
40-41	21.71948286964687	28.3812613843513	28.32271042115888	21.576545324842947
42-43	21.681669059989446	28.24151191027714	28.290853856840375	21.78596517289304
44-45	21.652657805085912	28.23563703619822	28.44501252568824	21.666692633027626
46-47	21.72906094034768	28.383283407111858	28.300523406265004	21.587132246275463
48-49	21.706631281315996	28.212114099686715	28.28073447212784	21.800520146869452
50-51	21.681411909260866	28.20767048598381	28.41775063036389	21.69316697439143
52-53	21.730218410841346	28.35290074514677	28.309228338847525	21.60765250516436
54-55	21.71618849448947	28.19863282120274	28.248726037831446	21.836452646476346
56-57	21.67899696765587	28.213039701174097	28.409735550208126	21.698227780961908
58-59	21.74560687491671	28.338627306882596	28.303784970084532	21.611980848116158
60-61	21.733723428591514	28.194906375023717	28.25347783314166	21.81789236324311
62-63	21.69278382125226	28.1977116914988	28.397992813631078	21.711511673617863
64-65	21.758323594981068	28.348577363390987	28.254185029185507	21.638914012442438
66-67	21.739266158141483	28.211098391666795	28.235369653011706	21.81426579718002
68-69	21.688897949928496	28.210391998236467	28.393959590195106	21.70675046163993
70-71	21.77602379710933	28.35851374103807	28.250184829560173	21.61527763229243
72-73	21.7222256301076	28.214473768841863	28.24451252888675	21.818788072163784
74-75	21.70240008156807	28.19094638625265	28.389274814922427	21.717378717256857
76-77	21.768914656233473	28.34586394317456	28.242085119495126	21.64313628109684
78-79	21.73574892255117	28.19173964712701	28.246544282032605	21.825967148289212
80-81	21.696662955440605	28.206614470144036	28.38748152703469	21.70924104738067
82-83	21.776601355473012	28.344900583194892	28.24135309145257	21.637144969879525
84-85	21.759321315210858	28.197191606618233	28.203128106694535	21.840358971476377
86-87	21.72509319377634	28.190401667991395	28.35290936533219	21.731595772900082
88-89	21.802808208223333	28.332680682748517	28.2166165445352	21.647894564492955
90-91	21.774472118143542	28.184611790037163	28.210807351529372	21.830108740289923
92-93	21.731744746004182	28.173006769826937	28.365767941782988	21.729480542385897
94-95	21.8166679650901	28.320489664630294	28.21850773903929	21.644334631240312
96-97	21.786497281895308	28.17636549247749	28.191035601578342	21.84610162404886
98-99	21.737821719581472	28.122123400146126	28.3541765761727	21.785878304099704
100	22.184054316393308	27.62741941545373	27.94322962428887	22.245296643864094
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	6.0
2	0.5
3	0.5
4	0.5
5	3.5
6	3.5
7	3.5
8	12.0
9	21.0
10	43.5
11	104.5
12	234.5
13	486.5
14	913.0
15	1733.0
16	3162.5
17	5599.5
18	9911.0
19	16900.5
20	27727.0
21	43964.5
22	66675.5
23	97515.0
24	139017.0
25	190320.5
26	247272.0
27	310437.5
28	381284.0
29	459074.5
30	543669.5
31	636283.0
32	739389.5
33	850651.5
34	970257.0
35	1101335.5
36	1243343.5
37	1394432.5
38	1543821.0
39	1691619.0
40	1837049.0
41	1970718.5
42	2078557.0
43	2149558.0
44	2180849.0
45	2166901.0
46	2112228.0
47	2010155.5
48	1868847.0
49	1712621.5
50	1545548.0
51	1369730.0
52	1197595.5
53	1033120.5
54	876690.5
55	734044.0
56	606917.0
57	494072.0
58	396094.0
59	312790.5
60	242428.0
61	184595.5
62	138451.0
63	102868.5
64	76696.0
65	57444.5
66	42427.5
67	31919.5
68	24676.5
69	19096.0
70	14817.5
71	11441.0
72	8767.0
73	6705.5
74	5175.0
75	3896.5
76	2795.0
77	2130.0
78	1593.5
79	1075.5
80	723.0
81	483.0
82	314.0
83	216.0
84	148.5
85	87.0
86	54.5
87	33.0
88	17.0
89	12.5
90	8.0
91	4.0
92	4.0
93	4.0
94	3.5
95	3.5
96	3.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0400979432891878
2	0.0018518371547315764
3	7.70024123013385E-4
4	0.059315477843902224
5	0.046201447380803104
6	0.312759705988728
7	0.03103716863925116
8	0.002810824252717571
9	0.23603837611266124
10-11	0.07533481404720523
12-13	0.1270114636278397
14-15	8.408852386281136E-4
16-17	0.018965977594282132
18-19	0.014254894424496255
20-21	0.16885140934255624
22-23	0.21750937539800327
24-25	0.03754458108987041
26-27	0.038471680685829776
28-29	0.0147261208433342
30-31	6.613704124041344E-4
32-33	0.031043073732219054
34-35	0.2726357802904814
36-37	0.04919060544115107
38-39	0.19919532006737664
40-41	0.09009282239256604
42-43	3.4603844791859177E-4
44-45	0.01400097542687681
46-47	0.1471336584252355
48-49	0.1285704081713637
50-51	0.033433455365622575
52-53	0.06921123263949908
54-55	0.10200339490880836
56-57	0.032201652972519874
58-59	0.24722970830541405
60-61	0.014152145806854897
62-63	0.014964686599237118
64-65	0.07445967926936332
66-67	0.04440393708137615
68-69	0.06085316405274184
70-71	0.027227205550391157
72-73	0.057464832566489946
74-75	0.014254897959909156
76-77	0.013965549817141645
78-79	0.18214151698123252
80-81	0.0018234948189953457
82-83	0.0457717117570268
84-85	0.031019663277746308
86-87	0.16062511187328107
88-89	0.03138140950467083
90-91	0.03462517425083215
92-93	0.10488389672359624
94-95	0.12176458391924543
96-97	0.08054200958052635
98-99	0.010604941800657072
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
69	4.0
70	1.0
71	3.0
72	0.0
73	2.0
74	1.0
75	3.0
76	2.0
77	3.0
78	11.0
79	6.0
80	26.0
81	50.0
82	63.0
83	64.0
84	95.0
85	102.0
86	108.0
87	124.0
88	187.0
89	407.0
90	538.0
91	838.0
92	1396.0
93	2546.0
94	5176.0
95	11001.0
96	26587.0
97	75979.0
98	291437.0
99	1819859.0
100	4.0099717E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.47539265106047
#Duplication Level	Percentage of total
1	62.34697606685613
2	21.455587831787508
3	8.976439970345334
4	3.4951907311208377
5	1.4922556587103322
6	0.6279505043442564
7	0.34100286028111504
8	0.21048314976951113
9	0.11998598768750068
>10	0.608468584247954
>50	0.22782643624913468
>100	0.09783221860042057
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.724074374315245E-6	2.3620371871576226E-6	4.724074374315245E-6	9.44814874863049E-6	4.724074374315245E-6	2.3620371871576227E-5
2	1.1810185935788114E-5	9.44814874863049E-6	9.44814874863049E-6	1.1810185935788114E-5	7.0861115614728686E-6	3.306852062020672E-5
3	1.4172223122945737E-5	1.653426031010336E-5	1.1810185935788114E-5	2.1258334684418606E-5	1.4172223122945737E-5	4.0154632181679586E-5
4	2.1258334684418606E-5	2.598240905873385E-5	1.653426031010336E-5	3.306852062020672E-5	2.1258334684418606E-5	4.0154632181679586E-5
5	2.8344446245891474E-5	2.598240905873385E-5	2.3620371871576227E-5	3.543055780736434E-5	3.070648343304909E-5	4.7240743743152455E-5
6	3.779259499452196E-5	3.070648343304909E-5	3.306852062020672E-5	4.0154632181679586E-5	3.779259499452196E-5	4.7240743743152455E-5
7	4.0154632181679586E-5	3.779259499452196E-5	3.306852062020672E-5	4.251666936883721E-5	4.0154632181679586E-5	5.432685530462532E-5
8	4.251666936883721E-5	4.487870655599483E-5	3.306852062020672E-5	4.487870655599483E-5	4.487870655599483E-5	5.905092967894057E-5
9	4.487870655599483E-5	4.960278093031008E-5	4.960278093031008E-5	4.7240743743152455E-5	4.960278093031008E-5	8.975741311198966E-5
10-11	4.605972514957364E-5	5.668889249178295E-5	5.314583671104651E-5	6.2593985459677E-5	5.432685530462532E-5	9.920556186062015E-5
12-13	4.842176233673127E-5	6.14129668660982E-5	6.849907842757106E-5	7.32231528018863E-5	5.786991108536176E-5	1.1810185935788113E-4
14-15	4.960278093031008E-5	6.968009702114987E-5	7.086111561472869E-5	8.38523201440956E-5	6.2593985459677E-5	1.3109306388724807E-4
16-17	5.5507873898204136E-5	7.32231528018863E-5	7.558518998904392E-5	9.448148748630491E-5	7.086111561472869E-5	1.4172223122945737E-4
18-19	6.023194827251938E-5	8.030926436335917E-5	8.857639451841085E-5	1.039296362349354E-4	7.558518998904392E-5	1.558944543524031E-4
20-21	7.086111561472869E-5	8.739537592483203E-5	9.802454326704135E-5	1.133777849835659E-4	9.093843170556847E-5	1.6652362169461242E-4
22-23	7.558518998904392E-5	8.857639451841085E-5	1.0983472920282946E-4	1.1928287795145995E-4	9.684352467346253E-5	1.8541991919187337E-4
24-25	8.149028295693798E-5	9.093843170556847E-5	1.133777849835659E-4	1.25187970919354E-4	1.0511065482851421E-4	2.173074212185013E-4
26-27	8.621435733125322E-5	9.920556186062015E-5	1.1810185935788113E-4	1.4054121263587857E-4	1.145588035771447E-4	2.2557455137355297E-4
28-29	9.566250607988372E-5	1.1101574779640827E-4	1.2164491513861757E-4	1.5471343575882427E-4	1.25187970919354E-4	2.409277930900775E-4
30-31	1.0511065482851421E-4	1.1928287795145995E-4	1.2636898951293282E-4	1.7006667747534885E-4	1.2755000810651162E-4	2.610051091809173E-4
32-33	1.0865371060925065E-4	1.2046389654503876E-4	1.3345510107440567E-4	1.7479075184966407E-4	1.3227408248082687E-4	2.7753936949102067E-4
34-35	1.1810185935788113E-4	1.2400695232577517E-4	1.4172223122945737E-4	1.8896297497260982E-4	1.3817917544872092E-4	3.011597413625969E-4
36-37	1.1810185935788113E-4	1.2636898951293282E-4	1.452652870101938E-4	1.9368704934692507E-4	1.44084268416615E-4	3.224180760470155E-4
38-39	1.1928287795145995E-4	1.2873102670009042E-4	1.5353241716524547E-4	2.078592724698708E-4	1.5353241716524547E-4	3.4131437354427646E-4
40-41	1.25187970919354E-4	1.3581713826156332E-4	1.5825649153956072E-4	2.1966945840565892E-4	1.5707547294598192E-4	3.625727082286951E-4
42-43	1.3109306388724807E-4	1.464463056037726E-4	1.653426031010336E-4	2.3147964434144702E-4	1.6416158450745477E-4	3.94460210255323E-4
44-45	1.3581713826156332E-4	1.5353241716524547E-4	1.747907518496641E-4	2.4328983027723514E-4	1.771527890368217E-4	4.3343382384342376E-4
46-47	1.3699815685514212E-4	1.5353241716524547E-4	1.866009377854522E-4	2.539189976194444E-4	1.866009377854522E-4	4.6295928868289403E-4
48-49	1.3936019404229975E-4	1.5707547294598192E-4	1.9250603075334627E-4	2.598240905873385E-4	1.984111237212403E-4	4.936657721159431E-4
50-51	1.4526528701019382E-4	1.6416158450745477E-4	2.0313519809555554E-4	2.680912207423902E-4	2.1140232825060722E-4	5.267342927361498E-4
52-53	1.5117037997808784E-4	1.7242871466250644E-4	2.0549723528271317E-4	2.7990140667817826E-4	2.173074212185013E-4	5.503546646077261E-4
54-55	1.5825649153956072E-4	1.7951482622397932E-4	2.1494538403134367E-4	2.8816853683323E-4	2.2557455137355297E-4	5.846042038215116E-4
56-57	1.6061852872671834E-4	1.8423890059829457E-4	2.267555699671318E-4	3.0706483433049094E-4	2.3266066293502582E-4	6.247588360031912E-4
58-59	1.6652362169461242E-4	2.0077316090839794E-4	2.480139046515504E-4	3.2359909464059434E-4	2.480139046515504E-4	6.44836152094031E-4
60-61	1.7124769606892767E-4	2.1140232825060722E-4	2.6218612777449614E-4	3.3186622479564596E-4	2.6454816496165374E-4	6.69637542559186E-4
62-63	1.747907518496641E-4	2.1848843981208012E-4	2.728152951167054E-4	3.460384479185917E-4	2.7163427652312664E-4	7.038870817729716E-4
64-65	1.7951482622397932E-4	2.3147964434144702E-4	2.8934955542680876E-4	3.5666761526080104E-4	2.8698751823965117E-4	7.487657883289664E-4
66-67	1.8187686341113694E-4	2.3620371871576227E-4	2.94073629801124E-4	3.649347454158527E-4	3.0824585292406977E-4	7.747481973877002E-4
68-69	1.8187686341113694E-4	2.444708488708139E-4	3.106078901112274E-4	3.743828941644832E-4	3.2359909464059434E-4	8.042736622271706E-4
70-71	1.8305788200471577E-4	2.4683288605797157E-4	3.2359909464059434E-4	3.861930801002713E-4	3.2950418760848836E-4	8.444282944088501E-4
72-73	1.8778195637903102E-4	2.610051091809173E-4	3.3186622479564596E-4	3.9800326603605946E-4	3.436764107314341E-4	8.692296848740052E-4
74-75	1.9132501215976744E-4	2.7045325792954776E-4	3.4721946651217054E-4	4.0272734041037466E-4	3.590296524479587E-4	8.881259823712662E-4
76-77	1.9841112372124032E-4	2.739963137102842E-4	3.6021067104153746E-4	4.098134519718475E-4	3.7792594994521964E-4	9.117463542428424E-4
78-79	1.9959214231481912E-4	2.8816853683323E-4	3.7202085697732556E-4	4.2398567509479326E-4	3.9446021025532304E-4	9.365477447079973E-4
80-81	2.0667825387629197E-4	3.023407599561757E-4	3.861930801002713E-4	4.393389168113178E-4	4.062703961911111E-4	9.57806079392416E-4
82-83	2.1494538403134367E-4	3.058838157369121E-4	3.9918428462963824E-4	4.440629911856331E-4	4.2280465650121443E-4	9.778833954832559E-4
84-85	2.2321251418639537E-4	3.1415094589196384E-4	4.0863243337826874E-4	4.558731771214212E-4	4.4170095399847543E-4	0.0010074088603227263
86-87	2.3266066293502582E-4	3.212370574534367E-4	4.2162363790763566E-4	4.665023444636305E-4	4.6414030727647286E-4	0.0010511065482851421
88-89	2.4092779309007752E-4	3.2714215042133077E-4	4.275287308755297E-4	4.783125303994186E-4	4.8421762336731263E-4	0.0010794509945310335
>>END_MODULE
