##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674191
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	47286968
Total Bases	4.7 Gbp
Sequences flagged as poor quality	0
Sequence length	63-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.145957275163	37.0	35.0	37.0	30.0	37.0
2	34.731456180485075	37.0	35.0	37.0	29.0	37.0
3	34.94708427911893	37.0	35.0	37.0	29.0	37.0
4	34.9331461048634	37.0	35.0	37.0	29.0	37.0
5	34.94151323045284	37.0	35.0	37.0	30.0	37.0
6	34.75556903965592	37.0	35.0	37.0	29.0	37.0
7	34.900793470200924	37.0	35.0	37.0	29.0	37.0
8	34.89881687910293	37.0	35.0	37.0	29.0	37.0
9	34.83045734714901	37.0	35.0	37.0	29.0	37.0
10-11	34.875590585549915	37.0	35.0	37.0	29.0	37.0
12-13	34.85453752289637	37.0	35.0	37.0	29.0	37.0
14-15	34.898695154233614	37.0	35.0	37.0	29.0	37.0
16-17	34.838306549914556	37.0	35.0	37.0	29.0	37.0
18-19	34.84970158162815	37.0	35.0	37.0	29.0	37.0
20-21	34.76363498289846	37.0	35.0	37.0	29.0	37.0
22-23	34.754861593156065	37.0	35.0	37.0	29.0	37.0
24-25	34.82150397124214	37.0	35.0	37.0	29.0	37.0
26-27	34.80848918035937	37.0	35.0	37.0	29.0	37.0
28-29	34.83125021887638	37.0	35.0	37.0	29.0	37.0
30-31	34.83685284072347	37.0	35.0	37.0	29.0	37.0
32-33	34.81260488090503	37.0	35.0	37.0	29.0	37.0
34-35	34.678748508468544	37.0	35.0	37.0	29.0	37.0
36-37	34.789716999829636	37.0	35.0	37.0	29.0	37.0
38-39	34.703880062684505	37.0	35.0	37.0	29.0	37.0
40-41	34.755493976691426	37.0	35.0	37.0	29.0	37.0
42-43	34.790960651145994	37.0	35.0	37.0	29.0	37.0
44-45	34.77561613804463	37.0	35.0	37.0	29.0	37.0
46-47	34.70204666114351	37.0	35.0	37.0	29.0	37.0
48-49	34.71573431182985	37.0	35.0	37.0	29.0	37.0
50-51	34.740704806871946	37.0	35.0	37.0	29.0	37.0
52-53	34.68520886135055	37.0	35.0	37.0	29.0	37.0
54-55	34.64852492762911	37.0	35.0	37.0	29.0	37.0
56-57	34.690283599912775	37.0	35.0	37.0	29.0	37.0
58-59	34.55413229285497	37.0	35.0	37.0	28.5	37.0
60-61	34.630766567651364	37.0	35.0	37.0	29.0	37.0
62-63	34.64689378054436	37.0	35.0	37.0	29.0	37.0
64-65	34.5946288329298	37.0	35.0	37.0	28.0	37.0
66-67	34.63180009655936	37.0	35.0	37.0	29.0	37.0
68-69	34.577050598098694	37.0	35.0	37.0	28.0	37.0
70-71	34.58266556045104	37.0	35.0	37.0	28.0	37.0
72-73	34.58900723404263	37.0	35.0	37.0	28.0	37.0
74-75	34.5659293630666	37.0	35.0	37.0	28.0	37.0
76-77	34.54855512807701	37.0	35.0	37.0	28.0	37.0
78-79	34.44311754704003	37.0	35.0	37.0	28.0	37.0
80-81	34.49372472967276	37.0	35.0	37.0	28.0	37.0
82-83	34.407148730510684	37.0	34.0	37.0	28.0	37.0
84-85	34.35691998755685	37.0	34.0	37.0	28.0	37.0
86-87	34.30121939090605	37.0	34.0	37.0	27.5	37.0
88-89	34.3042187836985	37.0	34.0	37.0	27.0	37.0
90-91	34.2552323317874	37.0	34.0	37.0	27.0	37.0
92-93	34.21331170106344	37.0	34.0	37.0	27.0	37.0
94-95	34.08370838328197	37.0	34.0	37.0	27.0	37.0
96-97	34.14179063428176	37.0	34.0	37.0	27.0	37.0
98-99	34.21662445569352	37.0	34.0	37.0	27.0	37.0
100	34.88806576782015	37.0	34.0	37.0	29.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	263.0
20	12104.0
21	62405.0
22	119081.0
23	180120.0
24	249181.0
25	327260.0
26	429316.0
27	565517.0
28	742121.0
29	969296.0
30	1265852.0
31	1664159.0
32	2248765.0
33	3222163.0
34	5141936.0
35	1.0293291E7
36	1.8929017E7
37	865121.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.939498773246978	32.21129626578461	17.84425521371093	27.004949747257484
2	26.337156030384453	28.754851419870437	27.466118387640254	17.44187416210486
3	18.13967502909079	27.778986486783698	30.270949934746632	23.81038854937888
4	19.133246051523663	28.535032316155977	29.383123646731146	22.94859798558921
5	21.79791789895626	27.296831770357983	28.624227305893506	22.281023024792244
6	21.787686469395393	26.899588631664674	29.102835846767718	22.209889052172215
7	22.603606317802182	28.04775663157392	27.64333976656332	21.705297284060578
8	20.55073423032308	26.259571114181202	29.817186143979146	23.372508511516575
9	21.66688171773594	25.726503243540343	29.8436554348959	22.762959603827813
10-11	22.657235377174683	26.45836932957135	27.86041310037598	23.02398219287798
12-13	22.785741122154697	26.933742490071293	27.29814334257033	22.98237304520368
14-15	22.85267057968915	27.020332420106776	27.226408989448377	22.9005880107557
16-17	22.870051704737225	26.979831558835144	27.26761946333753	22.8824972730901
18-19	22.83121374957199	26.88440994629458	27.29686588772211	22.987510416411318
20-21	22.940934498868582	26.85346156520233	27.20877425642812	22.99682967950097
22-23	22.970961394220332	26.99299511681063	27.085656422961392	22.95038706600765
24-25	22.943118319137707	27.00034965992145	27.06669682610423	22.989835194836612
26-27	23.01540077126031	26.9040400333864	27.122806938859107	22.95775225649418
28-29	23.062062242244625	26.87331361453672	27.061577427657173	23.003046715561478
30-31	23.039155301531306	26.876119938209197	27.020367842094288	23.06435691816521
32-33	23.08827439351873	26.888682920337526	26.98950676974562	23.033535916398122
34-35	23.07132242200891	26.90917022320515	26.990865091065885	23.02864226372005
36-37	23.051592752567263	26.92958106518531	26.9738674479356	23.044958734311834
38-39	23.126244539114982	26.827074483662937	26.99746852641589	23.049212450806188
40-41	23.13291992707604	26.814719459581788	26.974628642553544	23.07773197078863
42-43	23.073849115676502	26.864428543731307	26.938331881764473	23.12339045882772
44-45	23.100829090068604	26.847257867413187	26.977806653490422	23.07410638902779
46-47	23.12130762915336	26.841248848785632	26.975266774108047	23.062176747952964
48-49	23.07864279413052	26.851178190222562	26.959422563135572	23.110756452511342
50-51	23.136020658682728	26.81657306309248	26.9476201824093	23.099786095815496
52-53	23.131871856226066	26.80462493405517	26.95194941786026	23.111553791858498
54-55	23.082409480196105	26.850086256212375	26.921500527639363	23.146003735952153
56-57	23.118130517072544	26.839796138556	26.967805300248195	23.074268044123265
58-59	23.12747566723708	26.791691086423423	26.980310121511803	23.100523124827692
60-61	23.117711962346295	26.833861775890593	26.91095229833437	23.13747396342874
62-63	23.154950168129655	26.80821322328759	26.92734989564503	23.109486712937727
64-65	23.147683481959476	26.824271177486008	26.904450153815784	23.123595186738733
66-67	23.09788594777799	26.86607185851412	26.91600052097266	23.12004167273523
68-69	23.134200811278635	26.826466159915004	26.939160323630336	23.100172705176032
70-71	23.167481156621566	26.81231013149973	26.915025851540143	23.105182860338562
72-73	23.11640542069884	26.85824401016232	26.890257818917057	23.135092750221787
74-75	23.136272545196146	26.830229714937627	26.926501810049697	23.106995929816538
76-77	23.144846957201317	26.83245320571086	26.90972465673965	23.11297518034817
78-79	23.1078213284149	26.868231300292685	26.901239180594388	23.122708190698027
80-81	23.154773970410453	26.811369253301535	26.932451718485968	23.101405057802047
82-83	23.16532094883847	26.817314648716533	26.893358555345188	23.124005847099806
84-85	23.133392975534214	26.85438027103887	26.871545006071702	23.140681747355217
86-87	23.171911664082874	26.817828919362423	26.908570795174363	23.101688621380337
88-89	23.179142072909258	26.83293358445567	26.872861050334997	23.11506329230007
90-91	23.148523798449318	26.837847717536746	26.878562866417568	23.135065617596375
92-93	23.190655218474703	26.808548189124227	26.902964237299088	23.097832355101986
94-95	23.208735956121615	26.81405560136198	26.858627475974355	23.118580966542048
96-97	23.150318228698392	26.853213203564007	26.86024217820055	23.136226389537047
98-99	23.193580364454753	26.75840119101696	26.894970444047793	23.15304800048049
100	23.562647885541324	26.112461745960918	26.695688565583065	23.629201802914693
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	5.5
2	3.5
3	1.5
4	3.5
5	4.5
6	4.0
7	6.0
8	14.0
9	42.0
10	81.5
11	171.5
12	376.5
13	712.5
14	1283.0
15	2424.5
16	4414.0
17	7610.0
18	12292.5
19	19775.5
20	31403.5
21	46676.0
22	66806.0
23	93304.5
24	127017.5
25	169144.5
26	217408.5
27	271639.5
28	330644.5
29	393014.5
30	463647.0
31	542763.0
32	625930.5
33	711298.5
34	798105.5
35	887032.0
36	978404.0
37	1078900.0
38	1185135.0
39	1294867.5
40	1414720.5
41	1540318.5
42	1665929.5
43	1792728.5
44	1914919.5
45	2021320.5
46	2111056.0
47	2179950.0
48	2212595.5
49	2201451.5
50	2147540.0
51	2047832.5
52	1916889.5
53	1760143.5
54	1579227.0
55	1391571.5
56	1204186.5
57	1025305.5
58	863503.0
59	721759.0
60	602302.0
61	503007.0
62	420382.0
63	350202.5
64	289669.5
65	238411.0
66	194651.0
67	156752.0
68	124159.0
69	96912.5
70	74133.5
71	55228.0
72	40216.0
73	28297.5
74	19423.0
75	13064.5
76	8520.0
77	5452.5
78	3364.5
79	2044.5
80	1236.5
81	708.5
82	403.5
83	248.5
84	168.0
85	111.0
86	74.5
87	50.5
88	36.0
89	30.5
90	16.5
91	11.5
92	10.5
93	4.0
94	2.5
95	3.5
96	2.5
97	1.0
98	0.0
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04039167831610604
2	0.002260665137168448
3	0.0010996687290248763
4	0.05876671982860056
5	0.04717367372761138
6	0.31595808807196096
7	0.03109947755584583
8	0.002810499501680886
9	0.2371202991911006
10-11	0.07752664539625378
12-13	0.12774026873535219
14-15	8.839644783315353E-4
16-17	0.01923362902015625
18-19	0.014822265618721842
20-21	0.1726606789422405
22-23	0.22074263674507533
24-25	0.038450340059865966
26-27	0.03937871423686966
28-29	0.014882535924062629
30-31	6.746044703056453E-4
32-33	0.031548861411456956
34-35	0.2757525075407668
36-37	0.04864765277401588
38-39	0.19941435027088222
40-41	0.09085907136190251
42-43	4.22949511163414E-4
44-45	0.014680577532482099
46-47	0.14913834187888722
48-49	0.12890760938616325
50-51	0.03341935562457715
52-53	0.0707404204896368
54-55	0.10406884196931382
56-57	0.03246454710312575
58-59	0.25139273044530996
60-61	0.014458529039121308
62-63	0.01530125594011441
64-65	0.07571325096828477
66-67	0.04553157369483619
68-69	0.06126318644383217
70-71	0.027048685190809437
72-73	0.058488650933651326
74-75	0.01457061759254465
76-77	0.014510350020242906
78-79	0.18522457435482903
80-81	0.0019529730641873516
82-83	0.04635128247165814
84-85	0.031998582596655896
86-87	0.16048982095221845
88-89	0.03174002167715429
90-91	0.034124086327350665
92-93	0.10649857563402769
94-95	0.12152077482581797
96-97	0.08059081475717945
98-99	0.010733664984757835
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
63	1.0
64	0.0
65	0.0
66	1.0
67	2.0
68	3.0
69	4.0
70	1.0
71	4.0
72	1.0
73	3.0
74	5.0
75	4.0
76	5.0
77	6.0
78	12.0
79	14.0
80	47.0
81	86.0
82	103.0
83	133.0
84	127.0
85	131.0
86	197.0
87	220.0
88	262.0
89	481.0
90	665.0
91	919.0
92	1478.0
93	2641.0
94	5444.0
95	10988.0
96	26357.0
97	74188.0
98	293614.0
99	1927815.0
100	4.4941006E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.1914737779289
#Duplication Level	Percentage of total
1	69.93403039089964
2	9.544976943825926
3	5.890988470265201
4	4.206008445861295
5	2.8123239413959626
6	2.027137088546801
7	1.326722318672672
8	0.9618849656741275
9	0.6758444357101987
>10	2.5231933715377597
>50	0.07810377467500083
>100	0.018785852935567636
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.22949511163414E-6	6.344242667451209E-6	0.0	6.344242667451209E-6	1.2688485334902419E-5	1.480323289071949E-5
2	8.45899022326828E-6	6.344242667451209E-6	2.11474755581707E-6	1.2688485334902419E-5	3.595070844889019E-5	2.326222311398777E-5
3	1.480323289071949E-5	1.2688485334902419E-5	4.22949511163414E-6	2.326222311398777E-5	4.863919378379261E-5	2.749171822562191E-5
4	1.9032728002353628E-5	1.480323289071949E-5	6.344242667451209E-6	2.749171822562191E-5	6.555717423032916E-5	2.749171822562191E-5
5	2.5376970669804838E-5	2.11474755581707E-5	6.344242667451209E-6	3.383596089307312E-5	7.401616445359745E-5	4.018020356052433E-5
6	3.383596089307312E-5	2.11474755581707E-5	6.344242667451209E-6	3.595070844889019E-5	9.093414490013401E-5	4.652444622797554E-5
7	4.22949511163414E-5	2.11474755581707E-5	6.344242667451209E-6	4.4409698672158466E-5	1.0996687290248763E-4	5.286868889542675E-5
8	4.652444622797554E-5	2.960646578143898E-5	8.45899022326828E-6	4.863919378379261E-5	1.2265535823739005E-4	5.921293156287796E-5
9	5.921293156287796E-5	3.172121333725605E-5	1.057373777908535E-5	5.286868889542675E-5	1.3534384357229249E-4	7.401616445359745E-5
10-11	6.132767911869503E-5	3.8065456004707257E-5	1.480323289071949E-5	5.4983436451243824E-5	1.459175813513778E-4	8.77620235664084E-5
12-13	6.66145480082377E-5	4.969656756170114E-5	1.480323289071949E-5	5.8155557784969425E-5	1.691798044653656E-4	1.1736848934784739E-4
14-15	7.190141689778038E-5	6.449980045242063E-5	1.480323289071949E-5	6.238505289660355E-5	1.9138465380144485E-4	1.3745859112810954E-4
16-17	8.670464978849986E-5	7.718828578732305E-5	1.480323289071949E-5	6.555717423032916E-5	2.1041738180379844E-4	1.6495030935373146E-4
18-19	9.304889245595108E-5	8.247515467686573E-5	1.7975354224445094E-5	7.401616445359745E-5	2.2099111958288382E-4	1.9455677513517043E-4
20-21	1.0045050890131082E-4	9.304889245595108E-5	2.5376970669804838E-5	8.036040712104866E-5	2.3367960491778623E-4	2.178189982491582E-4
22-23	1.0679475156876204E-4	1.0150788267921935E-4	3.066383955934751E-5	8.776202356640839E-5	2.484828378085057E-4	2.3262223113987767E-4
24-25	1.1736848934784739E-4	1.057373777908535E-4	3.595070844889019E-5	9.199151867804255E-5	2.5799920180968254E-4	2.654008182550423E-4
26-27	1.2688485334902422E-4	1.1842586312575592E-4	3.91228297826158E-5	1.0150788267921935E-4	2.643434444771337E-4	2.8972041514693857E-4
28-29	1.332290960164754E-4	1.2054061068157299E-4	5.1811315117518216E-5	1.057373777908535E-4	2.791466773678532E-4	3.1615475959465196E-4
30-31	1.395733386839266E-4	1.2371273201529858E-4	5.921293156287796E-5	1.0996687290248763E-4	2.907777889248471E-4	3.299006187074629E-4
32-33	1.4486020757346928E-4	1.3217172223856685E-4	6.34424266745121E-5	1.1525374179203031E-4	3.055810218155666E-4	3.436464778202739E-4
34-35	1.543765715746461E-4	1.4274546001765223E-4	6.872929556405477E-5	1.300569746827498E-4	3.172121333725605E-4	3.7325294360171285E-4
36-37	1.7129455202118267E-4	1.5226182401882903E-4	7.613091200941451E-5	1.4063071246183515E-4	3.3518748759700555E-4	4.049741569389689E-4
38-39	1.8292566357817655E-4	1.5543394535255466E-4	8.353252845477426E-5	1.4274546001765223E-4	3.5422021559935925E-4	4.514986031669444E-4
40-41	1.9561414891307896E-4	1.5966344046418877E-4	8.670464978849986E-5	1.5014707646301197E-4	3.7642506493543843E-4	4.8004769517047483E-4
42-43	2.0618788669216434E-4	1.6600768313163999E-4	8.881939734431695E-5	1.6600768313163999E-4	4.0074466182733477E-4	5.107115347298223E-4
44-45	2.1887637202706671E-4	1.7658142091072534E-4	9.622101378967669E-5	1.8504041113399363E-4	4.240068849413225E-4	5.360885053996272E-4
46-47	2.3473697869569478E-4	1.8926990624562774E-4	1.0045050890131082E-4	1.934994013572619E-4	4.4621173427740175E-4	5.75211335182243E-4
48-49	2.4742546403059716E-4	1.9561414891307896E-4	1.0150788267921935E-4	1.9772889646889604E-4	4.641870885018468E-4	6.196210338544015E-4
50-51	2.5482708047595696E-4	1.9984364402471312E-4	1.0362263023503643E-4	2.030157653584387E-4	4.7899032139256636E-4	6.513422471916576E-4
52-53	2.675155658108594E-4	2.0407313913634723E-4	1.0996687290248765E-4	2.0407313913634723E-4	5.159984036193651E-4	6.788339654172794E-4
54-55	2.8443354625739593E-4	2.0724526047007286E-4	1.1736848934784739E-4	2.1358950313752408E-4	5.45604869400804E-4	7.190141689778038E-4
56-57	2.928925364806642E-4	2.146468769154326E-4	1.2265535823739005E-4	2.2099111958288382E-4	5.73096587626426E-4	7.761123529848647E-4
58-59	3.1509738581674343E-4	2.241632409166094E-4	1.2477010579320713E-4	2.2839273602824357E-4	5.91071941850871E-4	8.194646778791146E-4
60-61	3.214416284841946E-4	2.3685172625151184E-4	1.3217172223856688E-4	2.3473697869569478E-4	6.037604271857735E-4	8.511858912163706E-4
62-63	3.299006187074629E-4	2.4742546403059716E-4	1.3957333868392662E-4	2.4742546403059716E-4	6.259652765218528E-4	8.786776094419925E-4
64-65	3.394169827086397E-4	2.5376970669804843E-4	1.4591758135137784E-4	2.6222869692131665E-4	6.50284873413749E-4	9.241446818920595E-4
66-67	3.4893334670981657E-4	2.6222869692131665E-4	1.5014707646301197E-4	2.664581920329508E-4	6.894077031963648E-4	9.738412494537606E-4
68-69	3.531628418214507E-4	2.749171822562191E-4	1.564913191304632E-4	2.72802434700402E-4	7.41219018313883E-4	0.001023537817015462
70-71	3.6162183204471894E-4	2.8020405114576177E-4	1.5754869290837172E-4	2.876056675911215E-4	7.665959889836878E-4	0.0010658327681318032
72-73	3.658513271563531E-4	2.918351627027557E-4	1.6389293557582293E-4	3.055810218155666E-4	7.856287169860415E-4	0.0011271604472504982
74-75	3.690234484900787E-4	2.992367791481154E-4	1.723519257990912E-4	3.172121333725605E-4	8.02546697432578E-4	0.001175799641034291
76-77	3.7748243871334696E-4	3.0452364803765805E-4	1.755240471328168E-4	3.214416284841946E-4	8.332105369919256E-4	0.0012244388348180836
78-79	3.8805617649243234E-4	3.1509738581674343E-4	1.7975354224445094E-4	3.288432449295544E-4	8.522432649942793E-4	0.001268848533490242
80-81	3.912282978261579E-4	3.224990022621032E-4	1.8504041113399363E-4	3.383596089307312E-4	8.871365996652608E-4	0.001316430353496126
82-83	4.0814627827269446E-4	3.2778587115164586E-4	1.8926990624562774E-4	3.47875972931908E-4	9.114561965571572E-4	0.0013777580326148212
84-85	4.218921373855054E-4	3.34130113819097E-4	1.9455677513517043E-4	3.5844971071099336E-4	9.495216525618644E-4	0.0014327414690660648
86-87	4.324658751645908E-4	3.383596089307312E-4	1.9878627024680457E-4	3.647939533784446E-4	9.791281183433034E-4	0.0014940691481847598
88-89	4.38810117832042E-4	3.4258910404236535E-4	2.030157653584387E-4	3.6690870093426163E-4	0.0010182509481259192	0.0015532820797476378
>>END_MODULE
