##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674196
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	51189874
Total Bases	5.1 Gbp
Sequences flagged as poor quality	0
Sequence length	62-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.17141618672474	37.0	35.0	37.0	30.0	37.0
2	34.67969659390058	37.0	35.0	37.0	29.0	37.0
3	34.97396494861464	37.0	35.0	37.0	30.0	37.0
4	34.95749571487517	37.0	35.0	37.0	30.0	37.0
5	34.938933039764855	37.0	35.0	37.0	29.0	37.0
6	34.76570698337722	37.0	35.0	37.0	29.0	37.0
7	34.913276969581915	37.0	35.0	37.0	29.0	37.0
8	34.903397378942564	37.0	35.0	37.0	29.0	37.0
9	34.83875519990536	37.0	35.0	37.0	29.0	37.0
10-11	34.87973612710983	37.0	35.0	37.0	29.0	37.0
12-13	34.867205816916055	37.0	35.0	37.0	29.0	37.0
14-15	34.90331894546175	37.0	35.0	37.0	29.0	37.0
16-17	34.84589286154524	37.0	35.0	37.0	29.0	37.0
18-19	34.863035499950634	37.0	35.0	37.0	29.0	37.0
20-21	34.771239835440895	37.0	35.0	37.0	29.0	37.0
22-23	34.763374891291974	37.0	35.0	37.0	29.0	37.0
24-25	34.83589811141164	37.0	35.0	37.0	29.0	37.0
26-27	34.815733136987205	37.0	35.0	37.0	29.0	37.0
28-29	34.84025971620872	37.0	35.0	37.0	29.0	37.0
30-31	34.8509597327784	37.0	35.0	37.0	29.0	37.0
32-33	34.82075753888357	37.0	35.0	37.0	29.0	37.0
34-35	34.68604947142476	37.0	35.0	37.0	29.0	37.0
36-37	34.8021232871173	37.0	35.0	37.0	29.0	37.0
38-39	34.70816112967967	37.0	35.0	37.0	29.0	37.0
40-41	34.762518725871445	37.0	35.0	37.0	29.0	37.0
42-43	34.804475510137024	37.0	35.0	37.0	29.0	37.0
44-45	34.780353307765516	37.0	35.0	37.0	29.0	37.0
46-47	34.70830521090949	37.0	35.0	37.0	29.0	37.0
48-49	34.726714086071006	37.0	35.0	37.0	29.0	37.0
50-51	34.74589821221283	37.0	35.0	37.0	29.0	37.0
52-53	34.68982970147572	37.0	35.0	37.0	29.0	37.0
54-55	34.65960567709153	37.0	35.0	37.0	29.0	37.0
56-57	34.69336668029306	37.0	35.0	37.0	29.0	37.0
58-59	34.55440242927732	37.0	35.0	37.0	28.5	37.0
60-61	34.63902684151948	37.0	35.0	37.0	29.0	37.0
62-63	34.649198524306634	37.0	35.0	37.0	29.0	37.0
64-65	34.598471540961945	37.0	35.0	37.0	28.0	37.0
66-67	34.64019073500285	37.0	35.0	37.0	29.0	37.0
68-69	34.577417281532796	37.0	35.0	37.0	28.0	37.0
70-71	34.582706561474524	37.0	35.0	37.0	28.0	37.0
72-73	34.597139283887834	37.0	35.0	37.0	28.0	37.0
74-75	34.56584614907548	37.0	35.0	37.0	28.0	37.0
76-77	34.547876594439245	37.0	35.0	37.0	28.0	37.0
78-79	34.449623346696924	37.0	35.0	37.0	28.0	37.0
80-81	34.4907366885458	37.0	35.0	37.0	28.0	37.0
82-83	34.402834681952214	37.0	34.0	37.0	28.0	37.0
84-85	34.36074854592452	37.0	34.0	37.0	28.0	37.0
86-87	34.296849402940296	37.0	34.0	37.0	27.5	37.0
88-89	34.299006610803275	37.0	34.0	37.0	27.0	37.0
90-91	34.25614657145438	37.0	34.0	37.0	27.0	37.0
92-93	34.206572883775074	37.0	34.0	37.0	27.0	37.0
94-95	34.076475543755095	37.0	34.0	37.0	27.0	37.0
96-97	34.14291478924981	37.0	34.0	37.0	27.0	37.0
98-99	34.20708682653042	37.0	34.0	37.0	27.0	37.0
100	34.89129731231933	37.0	34.0	37.0	29.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	286.0
20	13542.0
21	69646.0
22	129702.0
23	192590.0
24	262822.0
25	343489.0
26	452667.0
27	599152.0
28	786764.0
29	1033113.0
30	1355735.0
31	1792658.0
32	2437445.0
33	3514411.0
34	5655732.0
35	1.134688E7
36	2.031199E7
37	891249.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	24.847522113048306	34.69995430651742	16.61643758006644	23.836086000367835
2	24.577843496968754	29.215995456509308	29.648919932463397	16.55724111405854
3	17.327663037955286	28.23708523052203	31.489412892973924	22.945838838548763
4	19.661421996340664	28.752501854247953	29.355310068666547	22.23076608074484
5	21.95039011887136	28.055042219106646	28.28636463623222	21.70820302578978
6	21.730582858462444	26.940289472499124	29.08429758648021	22.24483008255822
7	22.48688856540018	28.3220158098148	27.76116887716923	21.429926747615795
8	20.201530785025632	26.867409905117963	30.114935760873042	22.81612354898337
9	21.319567462712158	26.218720408630585	29.971493986060533	22.490218142596724
10-11	22.137069181547435	27.304460517090646	28.268730335631936	22.28973996572999
12-13	22.255368570518165	27.418315553429206	27.718548594772425	22.607767281280207
14-15	22.17134006764211	27.58277902603929	27.738644254116906	22.507236652201698
16-17	22.50861054038584	27.65415517251317	27.509377929916184	22.327856357184803
18-19	22.541596010746836	27.33055415706895	27.40724862135966	22.72060121082455
20-21	22.448541734722543	27.34197018352037	27.640263936794014	22.569224144963073
22-23	22.575116440640283	27.606762301141906	27.41788394987146	22.40023730834635
24-25	22.554004851267972	27.361498435416685	27.409162772019062	22.675333941296277
26-27	22.438060110185063	27.29158376853691	27.71789537060494	22.552460750673088
28-29	22.64488372957923	27.437651359470973	27.44543043558498	22.472034475364815
30-31	22.66377327525726	27.218721728530188	27.345315986568387	22.77218900964417
32-33	22.563025451474584	27.25770999573065	27.499390902677153	22.679873650117614
34-35	22.76001854240257	27.445080022804152	27.24959908748923	22.545302347304048
36-37	22.755489093375925	27.20548466993662	27.215485659188275	22.82354057749918
38-39	22.63978226640827	27.218964683767155	27.42977917553897	22.711473874285602
40-41	22.78870625411113	27.37934739846814	27.240534261203546	22.591412086217183
42-43	22.769887512439034	27.158190736176017	27.21504108446901	22.856880666915934
44-45	22.639066394132715	27.19538636439435	27.427547364992034	22.737999876480902
46-47	22.80883967316536	27.364474880291787	27.22538265621542	22.60130279032743
48-49	22.783292606518252	27.14337252454719	27.198714022647195	22.87462084628736
50-51	22.679930730393433	27.16033305503447	27.38957982447083	22.770156390101274
52-53	22.81310171040374	27.334425116794332	27.219796552625642	22.632676620176287
54-55	22.808511200828352	27.11988074946317	27.171095425792846	22.90051262391563
56-57	22.674196099643375	27.174195342414	27.38616778375913	22.765440774183503
58-59	22.83065230730322	27.31810205939331	27.22346257770315	22.627783055600318
60-61	22.82478344891487	27.11656619476791	27.16174662915167	22.896903727165547
62-63	22.702887068944616	27.15596442485176	27.362978000683796	22.77817050551983
64-65	22.83892403622424	27.336677855716406	27.173056062345307	22.651342045714046
66-67	22.821484233714624	27.129613322173597	27.157912997085965	22.890989447025817
68-69	22.695464738845217	27.165137005315486	27.36546610596046	22.773932149878835
70-71	22.851079630093462	27.33310648200129	27.187508805404974	22.62830508250027
72-73	22.834007816235204	27.11954395000174	27.153385699581197	22.89306253418186
74-75	22.71620341825644	27.140105862975407	27.356530669632384	22.787160049135768
76-77	22.857124435671484	27.31666149237308	27.165807225944956	22.660406846010485
78-79	22.833186026464663	27.10641409637538	27.16599932599047	22.89440055116949
80-81	22.716835427258708	27.144715306498668	27.36574222788284	22.772707038359787
82-83	22.872615618471553	27.318910610757435	27.15666199323912	22.651811777531897
84-85	22.860563174752095	27.10784069217491	27.126284919589487	22.905311213483508
86-87	22.741468921570238	27.14509297688131	27.31886508207352	22.79457301947494
88-89	22.905083037577064	27.314804777021696	27.141152385542938	22.6389597998583
90-91	22.866145888230605	27.100966436924733	27.141537755270573	22.891349919574086
92-93	22.745036015369312	27.13210235317936	27.336280779067245	22.78658085238408
94-95	22.918321688707806	27.28353524602235	27.13349947720084	22.664643588069005
96-97	22.88589079194842	27.090937237436997	27.118282628073615	22.904889342540972
98-99	22.756489083714534	27.090337517490422	27.328049884426107	22.825123514368933
100	23.38085001616318	26.59693524287463	26.797135979747928	23.225078761214263
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	6.5
2	2.0
3	2.0
4	6.5
5	6.5
6	9.0
7	23.0
8	46.0
9	153.0
10	329.0
11	633.0
12	1220.5
13	2153.0
14	3780.0
15	6477.5
16	10198.0
17	15439.0
18	22867.0
19	33381.5
20	48055.0
21	68701.5
22	96266.0
23	129604.0
24	171993.5
25	225501.0
26	288204.5
27	358164.0
28	433352.0
29	518276.5
30	611750.0
31	710650.5
32	815919.0
33	930092.0
34	1053230.0
35	1175746.5
36	1299570.5
37	1432125.0
38	1559403.0
39	1677938.5
40	1790928.0
41	1897650.5
42	1994181.0
43	2060381.5
44	2096702.0
45	2107750.5
46	2088325.0
47	2033081.5
48	1954538.5
49	1874917.5
50	1791955.0
51	1702463.0
52	1608339.0
53	1507440.5
54	1399033.5
55	1285574.5
56	1169752.5
57	1056463.0
58	945944.0
59	839066.5
60	737001.0
61	639784.0
62	550381.5
63	468778.5
64	395546.5
65	330151.0
66	273142.5
67	223420.5
68	179719.0
69	141126.5
70	107389.5
71	79433.5
72	57674.0
73	40739.0
74	27424.0
75	18088.5
76	11719.0
77	7377.0
78	4600.5
79	2749.0
80	1601.0
81	951.0
82	586.5
83	356.0
84	213.0
85	135.0
86	81.0
87	57.5
88	38.0
89	20.0
90	14.5
91	13.0
92	12.0
93	6.0
94	4.5
95	4.0
96	1.0
97	1.0
98	1.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04034196294368687
2	0.0020277447840563157
3	0.0010470820850232996
4	0.05922851070115937
5	0.046999529633536506
6	0.314351232823898
7	0.030943619826061693
8	0.0026704500190799455
9	0.23706250966743933
10-11	0.07739030574679671
12-13	0.12747540656185244
14-15	8.947081995161777E-4
16-17	0.019279203539356242
18-19	0.01477245284878021
20-21	0.171056291328242
22-23	0.21884113252554596
24-25	0.03817258858656304
26-27	0.039349579176537926
28-29	0.015048874705180949
30-31	6.807987064004103E-4
32-33	0.0308381302130183
34-35	0.2742622496003799
36-37	0.048711782334138976
38-39	0.19985300217773538
40-41	0.0897433347853132
42-43	4.180514294682577E-4
44-45	0.014661102701678851
46-47	0.14765810910181182
48-49	0.1279354584854028
50-51	0.03334643878982785
52-53	0.0702433844631069
54-55	0.10385843106392487
56-57	0.03218702980202686
58-59	0.2494155387059558
60-61	0.014300679857113927
62-63	0.015557764716470386
64-65	0.07496014201715803
66-67	0.045330257724819384
68-69	0.060658486936155276
70-71	0.027176277695044927
72-73	0.0579841375622384
74-75	0.014035006974135521
76-77	0.014330967121878039
78-79	0.18386169832350505
80-81	0.0019710959582648073
82-83	0.0457465549625689
84-85	0.03169991017382019
86-87	0.16014528392973323
88-89	0.032072469343954836
90-91	0.03415457707523024
92-93	0.10603628689487869
94-95	0.12119383725840023
96-97	0.08017079982501328
98-99	0.01034783255835592
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62	2.0
63	0.0
64	2.0
65	0.0
66	0.0
67	1.0
68	0.0
69	3.0
70	1.0
71	1.0
72	4.0
73	0.0
74	6.0
75	7.0
76	2.0
77	6.0
78	8.0
79	19.0
80	32.0
81	84.0
82	98.0
83	114.0
84	129.0
85	128.0
86	166.0
87	166.0
88	266.0
89	456.0
90	609.0
91	991.0
92	1587.0
93	2901.0
94	5830.0
95	11947.0
96	28277.0
97	80228.0
98	312510.0
99	2061654.0
100	4.8681639E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.09020772460515
#Duplication Level	Percentage of total
1	64.56736236837767
2	18.000977121766315
3	8.505060663885729
4	3.955755026082265
5	1.967783647079609
6	0.8920381414951588
7	0.40678147175875384
8	0.2724581283482129
9	0.12780810571481224
>10	0.7383768398922466
>50	0.1991222603601163
>100	0.35841143703188455
>500	0.0
>1k	0.008064788207208346
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	7.81404541062164E-6	9.76755676327705E-6	5.86053405796623E-6	1.758160217389869E-5	2.1488624879209508E-5	2.1488624879209508E-5
2	1.367457946858787E-5	1.172106811593246E-5	5.86053405796623E-6	2.344213623186492E-5	3.516320434779738E-5	3.711671570045279E-5
3	1.367457946858787E-5	1.562809082124328E-5	5.86053405796623E-6	3.516320434779738E-5	4.4930761111074426E-5	4.4930761111074426E-5
4	1.758160217389869E-5	1.562809082124328E-5	5.86053405796623E-6	4.883778381638525E-5	5.665182922700689E-5	4.688427246372984E-5
5	2.1488624879209508E-5	2.539564758452033E-5	9.76755676327705E-6	5.274480652169607E-5	7.032640869559476E-5	6.251236328497312E-5
6	2.539564758452033E-5	2.734915893717574E-5	9.76755676327705E-6	6.251236328497312E-5	7.81404541062164E-5	8.00939654588718E-5
7	2.539564758452033E-5	3.516320434779738E-5	9.76755676327705E-6	7.423343140090558E-5	8.986152222214885E-5	9.76755676327705E-5
8	3.125618164248656E-5	4.2977249758419016E-5	1.172106811593246E-5	8.400098816418262E-5	1.0548961304339214E-4	1.1916419251198E-4
9	4.102373840576361E-5	5.274480652169607E-5	1.172106811593246E-5	8.790801086949344E-5	1.1330365845401378E-4	1.5042037415446656E-4
10-11	4.102373840576361E-5	5.958209625599E-5	1.172106811593246E-5	1.0060583466175362E-4	1.2209445954096312E-4	1.6702522065203756E-4
12-13	4.6884272463729835E-5	6.739614166661165E-5	1.172106811593246E-5	1.2209445954096312E-4	1.3381552765689557E-4	1.9437437958921329E-4
14-15	6.055885193231771E-5	7.521018707723328E-5	1.2697823792260165E-5	1.377225503622064E-4	1.5139712983079428E-4	2.2367704987904445E-4
16-17	6.544263031395624E-5	9.083827789847657E-5	1.367457946858787E-5	1.5335064118344966E-4	1.7288575471000376E-4	2.637240326084803E-4
18-19	7.130316437192246E-5	9.962907898542591E-5	1.562809082124328E-5	1.7581602173898688E-4	1.933976239128856E-4	2.988872369562777E-4
20-21	7.91172097825441E-5	1.0255934601440902E-4	1.758160217389869E-5	2.0707220338147346E-4	2.0804895905780118E-4	3.4186448671469675E-4
22-23	8.888476654582115E-5	1.1330365845401378E-4	1.758160217389869E-5	2.2465380555537214E-4	2.3051433961333837E-4	3.8484173647311575E-4
24-25	9.66988119564428E-5	1.2209445954096312E-4	1.8558357850226394E-5	2.5395647584520325E-4	2.4321216340559854E-4	4.2391196352622394E-4
26-27	1.0060583466175362E-4	1.2307121521729084E-4	2.2465380555537213E-5	2.695845666664465E-4	2.5590998719785874E-4	4.66889213284643E-4
28-29	1.2014094818830771E-4	1.35769039009551E-4	2.344213623186492E-5	2.871661688403453E-4	2.8228239045870673E-4	5.049826846614234E-4
30-31	1.35769039009551E-4	1.5042037415446656E-4	2.539564758452033E-5	3.14515327777521E-4	3.0377101533791626E-4	5.381923776565654E-4
32-33	1.4065281739118952E-4	1.562809082124328E-4	2.930267028983115E-5	3.4381799806735213E-4	3.1744559480650415E-4	5.782393603860013E-4
34-35	1.4944361847813885E-4	1.6897873200469294E-4	3.320969299514197E-5	3.6823688997554473E-4	3.4284124239102444E-4	6.02658252294194E-4
36-37	1.6604846497570984E-4	1.7581602173898688E-4	3.711671570045279E-5	3.975395602653759E-4	3.6628337862288935E-4	6.368447009656637E-4
38-39	1.806998001206254E-4	1.8558357850226396E-4	4.102373840576361E-5	4.248887192025517E-4	3.867952478257712E-4	6.95450041545326E-4
40-41	1.933976239128856E-4	1.9925815797085182E-4	4.4930761111074426E-5	4.483308554344166E-4	3.9949307161803133E-4	7.296364902167956E-4
42-43	2.1390949311576738E-4	2.1293273743943969E-4	4.590751678740213E-5	4.620054349030045E-4	4.112141397339638E-4	7.667532059172483E-4
44-45	2.2563056123169983E-4	2.2367704987904445E-4	4.883778381638525E-5	4.815405484295585E-4	4.200049408209131E-4	8.087536999993397E-4
46-47	2.353981179949769E-4	2.314910952896661E-4	5.567507355067918E-5	4.952151278981464E-4	4.4149356570012265E-4	8.517309497577587E-4
48-49	2.490726974635648E-4	2.4321216340559854E-4	5.762858490333459E-5	5.157269971010283E-4	4.5614490084503823E-4	8.956849551925055E-4
50-51	2.607937655794972E-4	2.588402542268418E-4	6.544263031395624E-5	5.352621106275823E-4	4.756800143715923E-4	9.523367844195123E-4
52-53	2.6860781099011884E-4	2.695845666664465E-4	7.130316437192246E-5	5.655415365937411E-4	4.942383722218186E-4	9.845697217383265E-4
54-55	2.7642185640074047E-4	2.842359018113621E-4	7.325667572457787E-5	5.831231387676398E-4	5.088897073667342E-4	0.0010285237271730734
56-57	2.8716616884034524E-4	2.940034585746392E-4	8.497774384051033E-5	5.928906955309169E-4	5.215875311589944E-4	0.0010861523120764079
58-59	3.0572452669057164E-4	3.115850607485379E-4	9.474530060378739E-5	6.143793204101265E-4	5.3623886630391E-4	0.0011388971185981038
60-61	3.2818990724610886E-4	3.301434185987643E-4	9.86523233090982E-5	6.348911896130083E-4	5.528437128014809E-4	0.0011828511240328508
62-63	3.350271969804028E-4	3.4088773103836906E-4	1.0158259033808132E-4	6.593100815212008E-4	5.753090933570183E-4	0.0012355959305545468
64-65	3.457715094200075E-4	3.5260879915430146E-4	1.0548961304339214E-4	6.905662631636873E-4	5.928906955309169E-4	0.0012756429132839827
66-67	3.613996002412508E-4	3.6530662294656166E-4	1.1037339142503066E-4	7.061943539849306E-4	6.163328317627818E-4	0.0013147131403370908
68-69	3.711671570045279E-4	3.819114694441326E-4	1.1623392548299689E-4	7.227992004825017E-4	6.397749679946468E-4	0.0013567136344191824
70-71	3.819114694441326E-4	3.8972551485475426E-4	1.172106811593246E-4	7.364737799510896E-4	6.681008826081502E-4	0.0014075049295882228
72-73	3.9363253756006513E-4	3.926557818837374E-4	1.172106811593246E-4	7.579624048302991E-4	6.837289734293935E-4	0.0014612264917862466
74-75	4.0437684999966984E-4	3.955860489127205E-4	1.172106811593246E-4	7.706602286225592E-4	7.12054888042897E-4	0.0015217853437185644
76-77	4.1609791811560235E-4	3.9949307161803133E-4	1.2307121521729084E-4	7.980093875597349E-4	7.41357558332728E-4	0.0015735533945639326
78-79	4.200049408209131E-4	4.102373840576361E-4	1.2600148224627394E-4	8.253585464969107E-4	7.560088934776436E-4	0.0016380192692015613
80-81	4.2977249758419014E-4	4.23911963526224E-4	1.2893174927525706E-4	8.458704156997926E-4	7.833580524148194E-4	0.0016927175870759127
82-83	4.4247032137645034E-4	4.356330316421564E-4	1.3088526062791247E-4	8.693125519316574E-4	7.999628989123904E-4	0.0017435088822449533
84-85	4.522378781397274E-4	4.4637734408176116E-4	1.347922833332233E-4	8.927546881635223E-4	8.194980124389445E-4	0.0018099282682352373
86-87	4.6102867922667674E-4	4.580984121976936E-4	1.4065281739118952E-4	9.103362903374211E-4	8.527077054340864E-4	0.0018919757450467645
88-89	4.727497473426092E-4	4.678659689609707E-4	1.4455984009650034E-4	9.298714038639751E-4	8.888476654582115E-4	0.0019867210456505522
>>END_MODULE
