##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674198
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41833264
Total Bases	4.1 Gbp
Sequences flagged as poor quality	0
Sequence length	62-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.14776633255297	37.0	35.0	37.0	30.0	37.0
2	34.688734974158365	37.0	35.0	37.0	29.0	37.0
3	34.95278322054908	37.0	35.0	37.0	30.0	37.0
4	34.93223930602212	37.0	35.0	37.0	29.0	37.0
5	34.9228747486689	37.0	35.0	37.0	29.0	37.0
6	34.74413839665965	37.0	35.0	37.0	29.0	37.0
7	34.89350737728713	37.0	35.0	37.0	29.0	37.0
8	34.88648454493056	37.0	35.0	37.0	29.0	37.0
9	34.820964914427904	37.0	35.0	37.0	29.0	37.0
10-11	34.8662492843016	37.0	35.0	37.0	29.0	37.0
12-13	34.85138976963404	37.0	35.0	37.0	29.0	37.0
14-15	34.89148111894879	37.0	35.0	37.0	29.0	37.0
16-17	34.83293819722028	37.0	35.0	37.0	29.0	37.0
18-19	34.84995764375451	37.0	35.0	37.0	29.0	37.0
20-21	34.760416017263196	37.0	35.0	37.0	29.0	37.0
22-23	34.75394866391491	37.0	35.0	37.0	29.0	37.0
24-25	34.82534471849961	37.0	35.0	37.0	29.0	37.0
26-27	34.8075483662953	37.0	35.0	37.0	29.0	37.0
28-29	34.83285974290698	37.0	35.0	37.0	29.0	37.0
30-31	34.84429466225729	37.0	35.0	37.0	29.0	37.0
32-33	34.81486247403502	37.0	35.0	37.0	29.0	37.0
34-35	34.68361125251905	37.0	35.0	37.0	29.0	37.0
36-37	34.79797475281872	37.0	35.0	37.0	29.0	37.0
38-39	34.70588153245704	37.0	35.0	37.0	29.0	37.0
40-41	34.76334926913664	37.0	35.0	37.0	29.0	37.0
42-43	34.80288511553867	37.0	35.0	37.0	29.0	37.0
44-45	34.78460787807521	37.0	35.0	37.0	29.0	37.0
46-47	34.71426212403603	37.0	35.0	37.0	29.0	37.0
48-49	34.73108712004877	37.0	35.0	37.0	29.0	37.0
50-51	34.752841542558095	37.0	35.0	37.0	29.0	37.0
52-53	34.69916723447637	37.0	35.0	37.0	29.0	37.0
54-55	34.666292331384895	37.0	35.0	37.0	29.0	37.0
56-57	34.70557985865028	37.0	35.0	37.0	29.0	37.0
58-59	34.56958725477409	37.0	35.0	37.0	28.5	37.0
60-61	34.651053847961755	37.0	35.0	37.0	29.0	37.0
62-63	34.665939211376944	37.0	35.0	37.0	29.0	37.0
64-65	34.61752194262051	37.0	35.0	37.0	29.0	37.0
66-67	34.65785896546387	37.0	35.0	37.0	29.0	37.0
68-69	34.59945116154833	37.0	35.0	37.0	28.0	37.0
70-71	34.607709194883796	37.0	35.0	37.0	28.5	37.0
72-73	34.621877113450324	37.0	35.0	37.0	29.0	37.0
74-75	34.593819769740065	37.0	35.0	37.0	28.0	37.0
76-77	34.57958071573957	37.0	35.0	37.0	28.0	37.0
78-79	34.479764900974764	37.0	35.0	37.0	28.0	37.0
80-81	34.526423133097545	37.0	35.0	37.0	28.0	37.0
82-83	34.43939533191117	37.0	34.5	37.0	28.0	37.0
84-85	34.398264831921615	37.0	34.0	37.0	28.0	37.0
86-87	34.33937833420033	37.0	34.0	37.0	27.5	37.0
88-89	34.345204517497095	37.0	34.0	37.0	28.0	37.0
90-91	34.301627520961674	37.0	34.0	37.0	27.5	37.0
92-93	34.25817294833696	37.0	34.0	37.0	27.0	37.0
94-95	34.12899441534789	37.0	34.0	37.0	27.0	37.0
96-97	34.19602636295392	37.0	34.0	37.0	27.0	37.0
98-99	34.26201734965379	37.0	34.0	37.0	27.0	37.0
100	34.91812172344833	37.0	35.0	37.0	29.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	183.0
20	9515.0
21	50949.0
22	99371.0
23	152239.0
24	212966.0
25	281708.0
26	370499.0
27	492033.0
28	647854.0
29	849581.0
30	1110931.0
31	1466042.0
32	1986546.0
33	2850005.0
34	4577964.0
35	9179879.0
36	1.6755998E7
37	739001.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.001524995749286	34.818548941554425	18.250172001441538	23.929754061254755
2	24.0962590767686	29.588842698571955	30.04126625910617	16.273631965553275
3	17.2206415268573	28.57874271311389	31.69322294641377	22.50739281361504
4	19.014041992415287	29.13591201673007	30.090504491068664	21.759541499785975
5	21.25342654751091	28.41467228858577	29.063151980317386	21.26874918358594
6	20.832744726016493	27.621411994275608	29.982969694910327	21.562873584797572
7	21.641428441416505	29.01497057863821	28.564782447488295	20.778818532456995
8	19.665399203549768	27.383471236144434	30.834637634173816	22.116491926131975
9	20.68829766309033	26.734269490766273	30.645662343807533	21.931770502335866
10-11	21.653064567343243	27.72971250009838	28.798502132352123	21.818720800206254
12-13	21.696049674288094	27.97540813885923	28.317623028656524	22.010919158196153
14-15	21.684908634858573	28.09940997256811	28.345988707855213	21.869692684718103
16-17	21.83867540851049	28.136977469782178	28.267687915243112	21.756659206464228
18-19	21.816671255577536	27.92140459809706	28.23541257396802	22.026511572357386
20-21	21.785313575988056	27.891927122743525	28.366317290020575	21.95644201124784
22-23	21.97349196822463	28.11916020311464	28.067144423665287	21.840203404995446
24-25	21.974685583317047	27.92505303858348	28.01836273156411	22.081898646535365
26-27	21.93468608220338	27.897395563153175	28.182751845099137	21.98516650954431
28-29	22.09284782394745	28.017350701711024	27.96444948021209	21.92535199412944
30-31	22.045976409209196	27.854384595097	27.94932993002489	22.150309065668917
32-33	21.986331870619388	27.876119790607447	28.086172552900084	22.051375785873084
34-35	22.147109366002702	28.009478534957648	27.879814694579252	21.963597404460394
36-37	22.121221104253657	27.831285656625642	27.86193656254993	22.185556676570773
38-39	22.051606039585398	27.81911993735426	28.042479695716043	22.086794327344297
40-41	22.187317945756895	27.939927569851346	27.867654375597954	22.005100108793805
42-43	22.12729252845885	27.79579592946001	27.849670702328392	22.22724083975275
44-45	22.050935508748722	27.816892430345558	28.00300498904927	22.12916707185645
46-47	22.196817240856472	27.953710026819707	27.82749668130673	22.021976051017088
48-49	22.154638031927508	27.773032056696668	27.831873971742983	22.24045593963285
50-51	22.06894214905538	27.779745568963676	27.9976269813261	22.15368530065484
52-53	22.195362329220135	27.919003715968042	27.845111102587328	22.0405228522245
54-55	22.161076504953098	27.75086452115081	27.81674333963602	22.271315634260077
56-57	22.10069623204655	27.787423423011532	27.962960403473218	22.148919941468705
58-59	22.207549016870747	27.902258982387323	27.848225485813195	22.04196651492874
60-61	22.17926014188544	27.759086788403014	27.799554478855747	22.262098590855796
62-63	22.095854706890464	27.780366688683998	27.95622362380571	22.167554980619826
64-65	22.236263843848587	27.912521805669932	27.79061470976407	22.060599640717413
66-67	22.1722601746183	27.772456393553462	27.79923297930238	22.25605045252585
68-69	22.090419974938115	27.789091480712326	27.960796655946872	22.159691888402687
70-71	22.232239318635315	27.910792432268572	27.804440087549487	22.052528161546626
72-73	22.187295835147633	27.755632782332146	27.79610727774337	22.260964104776846
74-75	22.115396822174958	27.77338869719186	27.949379074000184	22.161835406632996
76-77	22.230084572577947	27.903050274893843	27.789930451809752	22.076934700718454
78-79	22.179861318442207	27.746031148454037	27.802437580252924	22.27166995285083
80-81	22.105820895691863	27.76935815907348	27.956170943313303	22.168650001921353
82-83	22.24545368830315	27.910588032434845	27.768297096347744	22.075661182914256
84-85	22.209193005198724	27.749397072244946	27.766086580227157	22.275323342329177
86-87	22.144806133299387	27.758578421718994	27.919562661875226	22.177052783106394
88-89	22.274065156599423	27.896619939277507	27.75676812194457	22.072546782178502
90-91	22.213655603365055	27.748042116677208	27.7763772428069	22.261925037150846
92-93	22.152996802016766	27.75569566725084	27.932278762285	22.159028768447396
94-95	22.290909306788166	27.87232410680757	27.758999453945638	22.077767132458625
96-97	22.23151159959076	27.734055350225876	27.753829709452667	22.280603340730693
98-99	22.148947033011073	27.71645974040031	27.920628667174004	22.213964559414613
100	22.645331055878973	27.250170333575785	27.498444709481667	22.606053901063575
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	6.5
2	4.0
3	2.0
4	3.0
5	3.5
6	7.0
7	17.0
8	26.0
9	53.0
10	105.5
11	187.5
12	341.5
13	808.0
14	1688.0
15	3255.5
16	5488.5
17	8704.0
18	13736.0
19	21201.0
20	32483.5
21	47882.5
22	69097.0
23	98012.0
24	135187.0
25	181785.0
26	238214.5
27	301889.5
28	369679.0
29	446027.0
30	531410.5
31	624355.0
32	725205.5
33	832707.0
34	948790.5
35	1072446.5
36	1203327.5
37	1333409.0
38	1461121.5
39	1581388.5
40	1684920.0
41	1775801.5
42	1848520.0
43	1894551.0
44	1910666.0
45	1897398.0
46	1858506.5
47	1790211.0
48	1695578.5
49	1587828.5
50	1468291.0
51	1340229.5
52	1210268.0
53	1079911.0
54	954538.0
55	838132.0
56	731850.0
57	636016.5
58	547550.5
59	468572.0
60	403081.5
61	346038.0
62	294364.0
63	249891.5
64	211333.0
65	177292.0
66	146707.0
67	119902.0
68	96762.0
69	76871.5
70	59860.0
71	45412.5
72	33764.5
73	24595.0
74	17322.5
75	11684.0
76	7800.0
77	5162.0
78	3294.5
79	2065.0
80	1293.0
81	774.5
82	488.0
83	314.5
84	181.5
85	109.5
86	76.0
87	51.5
88	27.0
89	12.0
90	11.0
91	11.5
92	8.0
93	5.5
94	3.5
95	3.5
96	4.0
97	3.0
98	2.0
99	3.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04004946876724704
2	0.0018717162495376885
3	9.77690863423901E-4
4	0.059646313995484555
5	0.046159439053094206
6	0.31487143819329994
7	0.031004035448919307
8	0.0027394467713540115
9	0.23573823931118545
10-11	0.07707024725586796
12-13	0.12654283921044268
14-15	9.047823760536592E-4
16-17	0.01910321891210784
18-19	0.014300820514507307
20-21	0.1712704033804295
22-23	0.21782426539798566
24-25	0.03840245408534223
26-27	0.03840603974865552
28-29	0.014946239910899613
30-31	5.856583411707966E-4
32-33	0.031212003921090165
34-35	0.2749068301244674
36-37	0.04893354723647669
38-39	0.19978598848992513
40-41	0.09008381464090394
42-43	3.370523514493155E-4
44-45	0.014716757458849015
46-47	0.14803650033141091
48-49	0.12794244312373043
50-51	0.033359816245751225
52-53	0.06855668732901167
54-55	0.10435714507000937
56-57	0.03247654784957731
58-59	0.24984662922787954
60-61	0.014506398544469301
62-63	0.0152175552834887
64-65	0.0749296134224299
66-67	0.044798089175819025
68-69	0.06107102777103872
70-71	0.027298857203383153
72-73	0.05786545114876558
74-75	0.014250626508242601
76-77	0.01414066764700342
78-79	0.18490922168811733
80-81	0.00161833187059957
82-83	0.04568751674810539
84-85	0.0316043053951688
86-87	0.16044984139739774
88-89	0.03134414396332812
90-91	0.03407829670048913
92-93	0.10585348356000623
94-95	0.12099509776906472
96-97	0.07989902716932934
98-99	0.010623592757021934
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62	1.0
63	2.0
64	2.0
65	1.0
66	1.0
67	0.0
68	0.0
69	4.0
70	0.0
71	1.0
72	0.0
73	3.0
74	1.0
75	2.0
76	4.0
77	9.0
78	6.0
79	15.0
80	25.0
81	58.0
82	78.0
83	91.0
84	104.0
85	93.0
86	127.0
87	153.0
88	215.0
89	385.0
90	547.0
91	733.0
92	1229.0
93	2233.0
94	4612.0
95	9239.0
96	22308.0
97	62693.0
98	245557.0
99	1630049.0
100	3.9852683E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.41040661170341
#Duplication Level	Percentage of total
1	77.3369786397927
2	12.880087720463914
3	5.198073019397023
4	2.088136459608599
5	0.9278320297328476
6	0.45743334197516333
7	0.2606963839531101
8	0.1874419538888258
9	0.15941506224297192
>10	0.4910239203455669
>50	0.0
>100	0.0013468269866768318
>500	0.0
>1k	0.01153464161258238
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.6733095462022758E-5	7.1713266265811816E-6	0.0	9.561768835441575E-6	1.6733095462022758E-5	3.3466190924045516E-5
2	2.8685306506324726E-5	7.1713266265811816E-6	4.780884417720788E-6	1.912353767088315E-5	4.541840196834749E-5	5.0199286386068274E-5
3	4.0637517550626695E-5	1.195221104430197E-5	9.561768835441575E-6	2.8685306506324726E-5	7.171326626581182E-5	6.215149743037025E-5
4	4.541840196834749E-5	2.1513979879743547E-5	1.912353767088315E-5	4.541840196834749E-5	1.0756989939871773E-4	6.693238184809103E-5
5	4.541840196834749E-5	2.390442208860394E-5	3.3466190924045516E-5	5.2589728594928667E-5	1.243029948607405E-4	7.410370847467222E-5
6	5.0199286386068274E-5	3.585663313290591E-5	4.0637517550626695E-5	6.454193963923063E-5	1.2908387927846126E-4	9.800813056327615E-5
7	5.2589728594928667E-5	4.3027959759487095E-5	5.2589728594928667E-5	6.932282405695143E-5	1.57769185784786E-4	1.0278901498099695E-4
8	5.737061301264945E-5	4.780884417720788E-5	5.976105522150985E-5	7.410370847467222E-5	1.8167360787338995E-4	1.195221104430197E-4
9	6.693238184809103E-5	5.498017080378906E-5	6.932282405695143E-5	7.64941506835326E-5	2.0318758775313348E-4	1.3625520590504245E-4
10-11	7.64941506835326E-5	6.334671853480044E-5	7.888459289239299E-5	1.0039857277213655E-4	2.2948245205059784E-4	1.6135484909807657E-4
12-13	9.203202504112517E-5	7.290848737024201E-5	9.442246724998557E-5	1.1474122602529892E-4	2.5936297966135276E-4	1.936258189176919E-4
14-15	1.0278901498099693E-4	7.529892957910241E-5	1.171316682341593E-4	1.3386476369618206E-4	3.023909394208398E-4	2.1513979879743547E-4
16-17	1.1474122602529892E-4	8.00798139968232E-5	1.3864564811390284E-4	1.4342653253162363E-4	3.466141202847571E-4	2.4023944199046962E-4
18-19	1.2669343706960088E-4	8.366547731011379E-5	1.601596279936464E-4	1.5418352247149541E-4	3.8605641673095364E-4	2.892435072721077E-4
20-21	1.3505998480061224E-4	9.442246724998557E-5	1.7330706014237858E-4	1.69721396829088E-4	4.3027959759487093E-4	3.3107624592716456E-4
22-23	1.5059785915820482E-4	1.0278901498099693E-4	1.8884493449997114E-4	1.8167360787338995E-4	4.709171151454976E-4	3.6693287906007044E-4
24-25	1.601596279936464E-4	1.159364471297291E-4	2.0557802996199387E-4	1.9243059781326173E-4	5.270925070537169E-4	4.1593694434170856E-4
26-27	1.6733095462022758E-4	1.2669343706960088E-4	2.3067767315502802E-4	2.007971455442731E-4	5.713156879176342E-4	4.5776968299676544E-4
28-29	1.7808794456009936E-4	1.3864564811390286E-4	2.438251053037602E-4	2.1394457769300526E-4	6.13148426572691E-4	4.91235873920811E-4
30-31	1.972114822309825E-4	1.4581697474048402E-4	2.5936297966135276E-4	2.2589678873730724E-4	6.502002808100272E-4	5.175307382182753E-4
32-33	2.1274935658857505E-4	1.565739646803558E-4	2.6653430628793395E-4	2.342633364683186E-4	6.932282405695142E-4	5.58168255768902E-4
34-35	2.2111590431958645E-4	1.6374529130693699E-4	2.796817384366661E-4	2.4741076861705075E-4	7.386466425378618E-4	5.892440044840871E-4
36-37	2.342633364683186E-4	1.7091661793351817E-4	2.9761005500311905E-4	2.5936297966135276E-4	7.721128334619073E-4	6.25100637616993E-4
38-39	2.450203264081904E-4	1.9243059781326173E-4	3.131479293607116E-4	2.701199696012245E-4	8.091646876992434E-4	6.848616928385029E-4
40-41	2.713151907056547E-4	1.984067033354127E-4	3.2749058261387397E-4	2.9163394948096804E-4	8.43826099727719E-4	7.254992103891296E-4
42-43	2.856578439588171E-4	2.043828088575637E-4	3.39442793658176E-4	3.143431504651418E-4	8.85658838382776E-4	7.709176123574771E-4
44-45	2.9761005500311905E-4	2.2948245205059784E-4	3.5498066801576853E-4	3.33466688136025E-4	9.251011348289724E-4	8.235073409524056E-4
46-47	3.1075748715185124E-4	2.390442208860394E-4	3.6573765795564026E-4	3.573711102246289E-4	9.502007780220066E-4	8.772922906517646E-4
48-49	3.227096981961532E-4	2.4741076861705075E-4	3.8605641673095364E-4	3.7768986899994227E-4	0.001000400064408075	9.203202504112517E-4
50-51	3.3227146703159474E-4	2.6055820076578294E-4	3.956181855663952E-4	3.88446858939814E-4	0.00103028059201883	9.729099790061803E-4
52-53	3.4183323586703634E-4	2.653390851835037E-4	4.147417232372784E-4	4.039847332974066E-4	0.0010709181095694565	0.0010159379387656674
54-55	3.4900456249361753E-4	2.7251041181008495E-4	4.422318086391729E-4	4.266939342815803E-4	0.0011067747427023623	0.0010565754563162941
56-57	3.585663313290591E-4	2.796817384366661E-4	4.5298879857904467E-4	4.4820791416132384E-4	0.0011390457125219776	0.0011187269537466643
58-59	3.8008031120880263E-4	2.832674017499567E-4	4.73307557354358E-4	4.637457885189164E-4	0.0011928306622213366	0.001177292787863744
60-61	3.956181855663952E-4	2.892435072721077E-4	4.8525976839865995E-4	4.864549895030902E-4	0.0012346634008763935	0.0012274920742498122
62-63	4.087656177151274E-4	2.9641483389868887E-4	5.043833060695431E-4	4.984072005473921E-4	0.0012753009184270202	0.0012776913606358806
64-65	4.1952260765499915E-4	2.9880527610754923E-4	5.282877281581471E-4	5.175307382182753E-4	0.0013183288781865071	0.001341038079170681
66-67	4.290843764904407E-4	3.0836704494299083E-4	5.426303814113094E-4	5.330686125758679E-4	0.0013541855113194132	0.001388846923347889
68-69	4.326700398037313E-4	3.143431504651418E-4	5.653395823954832E-4	5.474112658290303E-4	0.001409165682123202	0.0014282892197940854
70-71	4.4342702974360307E-4	3.203192559872928E-4	5.928296677973777E-4	5.665348034999134E-4	0.0014498031996738288	0.0014784885061801536
72-73	4.565744618923352E-4	3.239049193005834E-4	6.239054165125628E-4	5.784870145442154E-4	0.0014856598328067349	0.0015262973503573614
74-75	4.6733145183220704E-4	3.322714670315948E-4	6.573716074366083E-4	5.916344466929476E-4	0.00151673558152192	0.0015884488477877316
76-77	4.780884417720788E-4	3.3944279365817593E-4	6.800808084207821E-4	5.964153311106683E-4	0.0015633492045946976	0.0016350624708605095
78-79	4.888454317119506E-4	3.4541889918032694E-4	6.968139038828049E-4	6.13148426572691E-4	0.0016123532698763357	0.0016948235260820193
80-81	4.996024216518223E-4	3.585663313290591E-4	7.21913547075839E-4	6.25100637616993E-4	0.0016637477773668342	0.0017521941390946688
82-83	5.139450749049847E-4	3.633472157467799E-4	7.374514214334315E-4	6.358576275568649E-4	0.0016948235260820193	0.0018083695310028881
84-85	5.223116226359961E-4	3.7888509010437245E-4	7.529892957910242E-4	6.478098386011668E-4	0.001736656264737076	0.0019027919982528736
86-87	5.330686125758679E-4	3.932277433575348E-4	7.649415068353261E-4	6.573716074366083E-4	0.0017820746667054238	0.001960162611265523
88-89	5.390447180980188E-4	4.0637517550626696E-4	7.721128334619073E-4	6.693238184809103E-4	0.0018358596164047827	0.002025899772009184
>>END_MODULE
