##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674200
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	42304044
Total Bases	4.2 Gbp
Sequences flagged as poor quality	0
Sequence length	62-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.00121489567286	37.0	35.0	37.0	30.0	37.0
2	34.51912126887916	37.0	34.0	37.0	28.0	37.0
3	34.753796398282866	37.0	35.0	37.0	29.0	37.0
4	34.74931443906403	37.0	35.0	37.0	29.0	37.0
5	34.72997515320285	37.0	35.0	37.0	29.0	37.0
6	34.52885958136768	37.0	35.0	37.0	28.0	37.0
7	34.673177817231846	37.0	35.0	37.0	29.0	37.0
8	34.66581974999837	37.0	35.0	37.0	29.0	37.0
9	34.60750773613984	37.0	35.0	37.0	29.0	37.0
10-11	34.642301679243715	37.0	35.0	37.0	29.0	37.0
12-13	34.62037006438439	37.0	35.0	37.0	29.0	37.0
14-15	34.657523356395906	37.0	35.0	37.0	29.0	37.0
16-17	34.59200199631033	37.0	35.0	37.0	28.0	37.0
18-19	34.605315853491454	37.0	35.0	37.0	28.5	37.0
20-21	34.51505635016832	37.0	34.5	37.0	28.0	37.0
22-23	34.50725740309839	37.0	35.0	37.0	28.0	37.0
24-25	34.573946157487924	37.0	35.0	37.0	28.0	37.0
26-27	34.553975158970616	37.0	35.0	37.0	28.0	37.0
28-29	34.58036666660048	37.0	35.0	37.0	28.0	37.0
30-31	34.58849783959188	37.0	35.0	37.0	28.0	37.0
32-33	34.55673300405985	37.0	35.0	37.0	28.0	37.0
34-35	34.42150563667152	37.0	34.0	37.0	28.0	37.0
36-37	34.535187652509066	37.0	34.5	37.0	28.0	37.0
38-39	34.43575995949702	37.0	34.0	37.0	28.0	37.0
40-41	34.49582950036644	37.0	34.0	37.0	28.0	37.0
42-43	34.53095907332169	37.0	34.5	37.0	28.0	37.0
44-45	34.50810226322571	37.0	34.0	37.0	28.0	37.0
46-47	34.436710684207874	37.0	34.0	37.0	28.0	37.0
48-49	34.45005639413574	37.0	34.0	37.0	28.0	37.0
50-51	34.469916114875446	37.0	34.0	37.0	28.0	37.0
52-53	34.40864794155377	37.0	34.0	37.0	28.0	37.0
54-55	34.37332258353362	37.0	34.0	37.0	28.0	37.0
56-57	34.4110014163185	37.0	34.0	37.0	28.0	37.0
58-59	34.271788070662936	37.0	34.0	37.0	27.5	37.0
60-61	34.34662116463381	37.0	34.0	37.0	28.0	37.0
62-63	34.36093137300465	37.0	34.0	37.0	28.0	37.0
64-65	34.30852101103061	37.0	34.0	37.0	27.0	37.0
66-67	34.3484642046371	37.0	34.0	37.0	28.0	37.0
68-69	34.28471154623416	37.0	34.0	37.0	27.0	37.0
70-71	34.290999400209614	37.0	34.0	37.0	27.0	37.0
72-73	34.302707243766626	37.0	34.0	37.0	27.0	37.0
74-75	34.26900231328435	37.0	34.0	37.0	27.0	37.0
76-77	34.251654783498715	37.0	34.0	37.0	27.0	37.0
78-79	34.14732006635442	37.0	34.0	37.0	27.0	37.0
80-81	34.18805212947417	37.0	34.0	37.0	27.0	37.0
82-83	34.09509334838165	37.0	34.0	37.0	27.0	37.0
84-85	34.04323360109796	37.0	34.0	37.0	26.5	37.0
86-87	33.98501455773552	37.0	34.0	37.0	26.5	37.0
88-89	33.98570458411772	37.0	34.0	37.0	26.0	37.0
90-91	33.93442683005043	37.0	34.0	37.0	26.0	37.0
92-93	33.889760663808744	37.0	33.5	37.0	26.0	37.0
94-95	33.74737071234367	36.5	33.0	37.0	26.0	37.0
96-97	33.81764562982331	37.0	33.0	37.0	26.0	37.0
98-99	33.9009259358579	37.0	33.0	37.0	26.0	37.0
100	34.68093624100695	37.0	34.0	37.0	28.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	324.0
20	15377.0
21	79497.0
22	146564.0
23	210879.0
24	281019.0
25	360491.0
26	464303.0
27	600027.0
28	775733.0
29	997455.0
30	1289804.0
31	1685776.0
32	2266160.0
33	3216356.0
34	4998613.0
35	9259298.0
36	1.4944782E7
37	711585.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	24.227857121557225	31.163874834143147	18.65564155448482	25.952626489814808
2	25.123878610710047	28.95168737728147	28.262659956930825	17.661774055077657
3	16.61755659233605	27.935513207289553	31.70422248617659	23.742707714197806
4	18.90155800175917	28.821994204786357	29.874009875534508	22.40243791791997
5	21.56194981777636	27.748990217632063	28.85289375718313	21.83616620740845
6	21.27630342984779	27.475121134505002	29.31581406688741	21.932761368759795
7	22.10483248111181	28.782072279807714	28.208222285850393	20.90487295323009
8	19.789336796982642	26.857700359317526	30.458572487125846	22.894390356573986
9	21.06080679641958	26.210179820552632	30.33187752749554	22.397135855532248
10-11	22.265452203011378	27.03470978206703	28.225127240237764	22.474710774683828
12-13	22.37874722856886	27.38016395707995	27.64598087618283	22.595107938168365
14-15	22.38059505539138	27.524709900024803	27.624540430182787	22.470154614401032
16-17	22.52395488004783	27.576972697545486	27.53065262969498	22.3684197927117
18-19	22.472929137982728	27.444775129413728	27.53247063659796	22.54982509600558
20-21	22.42844245114693	27.382926585609553	27.648458591912707	22.54017237133081
22-23	22.567497927526496	27.545751806279984	27.440965849704185	22.445784416489335
24-25	22.547689176662264	27.44463022444777	27.43443711437535	22.57324348451462
26-27	22.533985771225808	27.388823660864723	27.553311790349643	22.523878777559826
28-29	22.67321568098476	27.461482011155336	27.38291067233852	22.48239163552138
30-31	22.59333929521382	27.3597363960488	27.39828970075277	22.64863460798461
32-33	22.553854422544507	27.366427849645415	27.494335391410413	22.585382336399658
34-35	22.6656931317899	27.47680355590535	27.350635360570624	22.506867951734126
36-37	22.624754074094362	27.367610572324203	27.37218327052761	22.635452083053824
38-39	22.59173904764896	27.32934811296419	27.476097287449015	22.602815551937837
40-41	22.69368512024254	27.406866914253804	27.33524302761367	22.564204937889983
42-43	22.62531870473587	27.335436049727907	27.34814411279181	22.691101132744407
44-45	22.569792114716186	27.340156765866464	27.461627279327534	22.628423840089816
46-47	22.686553108697865	27.444510826540586	27.339164199669717	22.52977186509183
48-49	22.639916674155895	27.327266413704642	27.360013502626785	22.672803409512674
50-51	22.601927831604794	27.300957956932358	27.455102338035964	22.642011873426885
52-53	22.692346231401075	27.384547175151052	27.35739740283776	22.56570919061011
54-55	22.622337573023046	27.317449436723084	27.351800070085513	22.708412920168357
56-57	22.586063754725043	27.34726341567463	27.454603213104857	22.61206961649547
58-59	22.689789364922657	27.406176713725294	27.366492148023656	22.53754177332839
60-61	22.6459221554091	27.312772856871636	27.350963642969766	22.690341344749495
62-63	22.602921122100017	27.304091141245113	27.456296307539407	22.636691429115466
64-65	22.70528339391474	27.41149417652158	27.320343812035443	22.56287861752824
66-67	22.64034276094307	27.323203007519332	27.354653717081177	22.68180051445642
68-69	22.591299760620547	27.336139920661612	27.460936880499627	22.611623438218214
70-71	22.715946545348032	27.420165931644398	27.335594391037144	22.52829313197043
72-73	22.631232444235465	27.320966679880303	27.351280382293474	22.696520493590757
74-75	22.613078329566992	27.296004566062958	27.455496188231322	22.635420916138724
76-77	22.707675834867533	27.40509459023605	27.331454171023395	22.55577540387302
78-79	22.642775391044196	27.307599064534216	27.36282183989068	22.686803704530906
80-81	22.602840256106653	27.303956298107963	27.463211551799787	22.629991893985597
82-83	22.733220039835572	27.396721572315442	27.315974620859617	22.55408376698937
84-85	22.67023146865464	27.298370242352892	27.329974251203954	22.701424037788513
86-87	22.633142723145344	27.293472616897436	27.433542568530694	22.639842091426523
88-89	22.733263116097834	27.378571412356745	27.323665623449976	22.564499848095444
90-91	22.681738577902202	27.290104751422657	27.342062194867665	22.686094475807476
92-93	22.654455665538798	27.278850580408655	27.446705520949287	22.61998823310326
94-95	22.77125292971301	27.35225151891504	27.302830801148385	22.573664750223564
96-97	22.691606737024774	27.280787891898616	27.317877632815424	22.709727738261183
98-99	22.645881093979124	27.231799247972038	27.435965055919503	22.686354602129335
100	23.16723143963177	26.626690033360852	27.055160897106884	23.150917629900494
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	4.5
2	2.5
3	3.5
4	4.0
5	5.0
6	7.0
7	9.5
8	31.0
9	67.0
10	108.5
11	249.5
12	521.0
13	960.0
14	1822.5
15	3337.0
16	5612.5
17	9271.0
18	15065.0
19	23176.0
20	34886.0
21	51420.5
22	72602.0
23	99580.5
24	134292.5
25	176704.5
26	227616.0
27	286622.5
28	350977.0
29	420453.0
30	497065.0
31	579157.0
32	664352.0
33	756655.0
34	855063.0
35	956906.5
36	1067079.0
37	1180960.0
38	1294340.5
39	1410768.0
40	1519510.0
41	1616980.5
42	1706781.5
43	1781593.5
44	1835509.0
45	1864335.5
46	1863257.5
47	1829168.0
48	1766484.0
49	1681940.5
50	1574293.5
51	1450634.5
52	1322210.0
53	1192833.5
54	1066792.5
55	945366.0
56	834080.5
57	735074.0
58	648708.5
59	575853.0
60	511965.5
61	454926.0
62	402896.0
63	354178.0
64	307711.5
65	264556.5
66	224237.0
67	186378.5
68	152208.5
69	121654.5
70	95429.5
71	73350.0
72	54986.5
73	40169.0
74	28487.0
75	19712.5
76	13404.0
77	8992.0
78	5966.5
79	3877.0
80	2438.5
81	1503.5
82	927.5
83	566.5
84	348.0
85	211.5
86	130.5
87	92.5
88	77.5
89	44.0
90	20.0
91	21.0
92	16.0
93	9.5
94	8.0
95	8.0
96	6.5
97	2.0
98	1.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03977161143270369
2	0.002217282111374506
3	0.0010306343289544612
4	0.059133826543864225
5	0.045362093515220435
6	0.3108497145095632
7	0.031205054533320738
8	0.0025293090183056734
9	0.23512882125406262
10-11	0.07550223803662837
12-13	0.12654936724252652
14-15	8.320717518164457E-4
16-17	0.019197928216980863
18-19	0.014140492100471528
20-21	0.16983482713851186
22-23	0.2170088041701167
24-25	0.0370531951980761
26-27	0.03898918032517175
28-29	0.01460262286035822
30-31	6.559656566166582E-4
32-33	0.030609366801906693
34-35	0.2730696384487497
36-37	0.04839962817739127
38-39	0.19866894994719653
40-41	0.09010131513668054
42-43	4.5740307947864274E-4
44-45	0.01447733932954495
46-47	0.14671174226274916
48-49	0.12879856119665534
50-51	0.033441247366327434
52-53	0.06923215189545473
54-55	0.10120663641518528
56-57	0.0320974042103398
58-59	0.24824695246629377
60-61	0.013907653840375168
62-63	0.01543705863483239
64-65	0.07465598853783259
66-67	0.04450284075698584
68-69	0.0609067131757004
70-71	0.027037610644885544
72-73	0.05784082646123376
74-75	0.013899386149448446
76-77	0.013992759364582401
78-79	0.1838324742086184
80-81	0.001918259749082406
82-83	0.04555496616855586
84-85	0.030919353650295633
86-87	0.16078752544952474
88-89	0.031260241605925415
90-91	0.0343057029366067
92-93	0.10433460292856747
94-95	0.12050115798884267
96-97	0.0801677299522499
98-99	0.009937681656322481
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62	1.0
63	2.0
64	0.0
65	0.0
66	0.0
67	1.0
68	1.0
69	4.0
70	2.0
71	3.0
72	2.0
73	1.0
74	1.0
75	2.0
76	4.0
77	9.0
78	11.0
79	15.0
80	33.0
81	63.0
82	98.0
83	135.0
84	110.0
85	121.0
86	162.0
87	192.0
88	270.0
89	486.0
90	621.0
91	951.0
92	1557.0
93	2861.0
94	5911.0
95	12246.0
96	28559.0
97	79847.0
98	305840.0
99	1903912.0
100	3.996001E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.31186445315228
#Duplication Level	Percentage of total
1	75.57579757121125
2	13.960807534558969
3	5.1479636166288865
4	2.4070316192476136
5	1.0804292278114218
6	0.5720178950545273
7	0.29288924952160067
8	0.17936732634586616
9	0.13216221688202473
>10	0.6452699414702325
>50	0.0021782051969303742
>100	0.004085596070645531
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	7.0915206120719804E-6	4.727680408047987E-6	9.455360816095973E-6	1.1819201020119968E-5	1.1819201020119968E-5	2.3638402040239936E-5
2	7.0915206120719804E-6	9.455360816095973E-6	1.4183041224143961E-5	2.127456183621594E-5	3.7821443264383893E-5	3.7821443264383893E-5
3	7.0915206120719804E-6	1.6546881428167956E-5	1.6546881428167956E-5	3.7821443264383893E-5	5.6732164896575844E-5	5.200448448852786E-5
4	9.455360816095973E-6	2.127456183621594E-5	1.6546881428167956E-5	4.491296387645588E-5	8.509824734486377E-5	5.6732164896575844E-5
5	1.1819201020119968E-5	2.8366082448287922E-5	2.600224224426393E-5	4.9640644284503867E-5	1.1819201020119968E-4	6.145984530462383E-5
6	1.4183041224143961E-5	3.5457603060359906E-5	2.8366082448287922E-5	6.382368550864783E-5	1.4183041224143962E-4	6.855136591669582E-5
7	1.4183041224143961E-5	3.7821443264383893E-5	2.8366082448287922E-5	6.855136591669582E-5	1.5601345346558356E-4	7.800672673279178E-5
8	1.6546881428167956E-5	3.7821443264383893E-5	3.0729922652311916E-5	8.273440714083977E-5	1.7256033489375153E-4	8.273440714083977E-5
9	1.8910721632191947E-5	4.9640644284503867E-5	3.7821443264383893E-5	8.746208754888776E-5	1.9856257713801547E-4	1.1110048958912769E-4
10-11	2.482032214225193E-5	5.791408499858784E-5	4.6094883978467875E-5	1.0400896897705571E-4	2.2692865958630337E-4	1.2528353081327166E-4
12-13	3.0729922652311916E-5	6.736944581468382E-5	5.0822564386515864E-5	1.3001121122131965E-4	2.564766621366033E-4	1.4064849213942763E-4
14-15	3.1911842754323914E-5	8.155248703882777E-5	5.5550244794563846E-5	1.477400127514996E-4	2.8247890438086725E-4	1.536496132615596E-4
16-17	4.254912367243188E-5	9.455360816095974E-5	6.145984530462383E-5	1.7374225499576352E-4	2.990257858090352E-4	1.808337756078355E-4
18-19	5.318640459053985E-5	1.075547292830917E-4	6.973328601870781E-5	1.9383489672996748E-4	3.214822677472631E-4	1.9974449724002746E-4
20-21	6.145984530462383E-5	1.2055585040522367E-4	8.037056693681578E-5	2.0683601785209942E-4	3.4748450999152705E-4	2.198371389742314E-4
22-23	6.618752571267182E-5	1.3473889162936764E-4	8.864400765089976E-5	2.4465746111648333E-4	3.74668672337803E-4	2.3638402040239935E-4
24-25	6.618752571267182E-5	1.489219328535116E-4	9.337168805894775E-5	2.7420546366678327E-4	4.195816362142588E-4	2.635681827486753E-4
26-27	6.855136591669582E-5	1.5837729366960756E-4	9.809936846699574E-5	3.049353863190952E-4	4.4676579856053476E-4	2.954800255029992E-4
28-29	7.68248066307798E-5	1.7374225499576352E-4	1.0400896897705571E-4	3.273918682573231E-4	4.940426026410147E-4	3.167545873392151E-4
30-31	8.509824734486377E-5	1.867433761178955E-4	1.1110048958912769E-4	3.5103027029756304E-4	5.188629247832666E-4	3.3684722907341907E-4
32-33	8.982592775291175E-5	1.9974449724002746E-4	1.1464624989516368E-4	3.71122912031767E-4	5.614120484556985E-4	3.5221219039957503E-4
34-35	9.691744836498374E-5	2.0919985805612341E-4	1.2410161071125966E-4	3.8294211305188696E-4	5.909600510059984E-4	3.7939635274585097E-4
36-37	9.691744836498374E-5	2.174732987702074E-4	1.2882929111930765E-4	4.006709145820669E-4	6.335091746784302E-4	4.077624351941389E-4
38-39	1.0164512877303171E-4	2.2692865958630337E-4	1.4301233234345162E-4	4.1839971611224683E-4	6.536018164126342E-4	4.5385731917260675E-4
40-41	1.1464624989516368E-4	2.2929249979032737E-4	1.501038529555236E-4	4.384923578464508E-4	7.044243807991501E-4	4.916787624369907E-4
42-43	1.1701009009918768E-4	2.4111170081044734E-4	1.5128577305753557E-4	4.680403603967507E-4	7.36336223553474E-4	5.283182855993626E-4
44-45	1.2055585040522367E-4	2.493851415245313E-4	1.5955921377161956E-4	4.869510820289427E-4	7.717938266138339E-4	5.779589298838665E-4
46-47	1.2646545091528366E-4	2.623862626466633E-4	1.6310497407765555E-4	5.141352443752186E-4	7.989779889601099E-4	6.110526927402023E-4
48-49	1.3355697152735564E-4	2.8720658478891524E-4	1.6901457458771553E-4	5.401374866194826E-4	8.356175121224817E-4	6.559656566166582E-4
50-51	1.4064849213942763E-4	3.013896260130592E-4	1.7610609519978752E-4	5.637758886597225E-4	8.710751151828416E-4	6.926051797790301E-4
52-53	1.4301233234345162E-4	3.1084498682915513E-4	1.855614560158835E-4	5.803227700878905E-4	9.053507981411895E-4	7.37518143655486E-4
54-55	1.477400127514996E-4	3.214822677472631E-4	1.9147105652594348E-4	6.075069324341664E-4	9.443541615075854E-4	7.836130276339539E-4
56-57	1.501038529555236E-4	3.333014687673831E-4	2.0329025754606346E-4	6.394187751884902E-4	9.880852052820293E-4	8.427090327345536E-4
58-59	1.5955921377161956E-4	3.4630258988951505E-4	2.0919985805612341E-4	6.748763782488501E-4	0.0010259066485464132	8.793485558969256E-4
60-61	1.6074113387363155E-4	3.65213311521707E-4	2.139275384641714E-4	6.937870998810421E-4	0.0010507269706886652	9.171699991613095E-4
62-63	1.6546881428167954E-4	3.7939635274585097E-4	2.2220097917825539E-4	7.20971262227318E-4	0.001095639934565121	9.774479243639213E-4
64-65	1.6783265448570354E-4	3.9476131407200694E-4	2.3047441989233937E-4	7.36336223553474E-4	0.001138189058237553	0.0010341800892604972
66-67	1.7374225499576352E-4	4.065805150921269E-4	2.3638402040239935E-4	7.5761078538969E-4	0.001171282821093889	0.001098003774769145
68-69	1.831976158118595E-4	4.148539558062109E-4	2.4702130132050733E-4	7.836130276339539E-4	0.0012031946638482128	0.001151190179359685
70-71	1.855614560158835E-4	4.337646774384028E-4	2.576585822386153E-4	8.096152698782178E-4	0.0012457437875206445	0.0011961031432361408
72-73	1.9147105652594348E-4	4.384923578464508E-4	2.694777832587353E-4	8.403451925305297E-4	0.0012906567513971004	0.0012421980272146085
74-75	1.9974449724002746E-4	4.5149347896858275E-4	2.7538738376879526E-4	8.663474347747936E-4	0.0013225685941514243	0.0012906567513971004
76-77	2.139275384641714E-4	4.5976691968266674E-4	2.8011506417684325E-4	8.935315971210695E-4	0.0013497527564977003	0.0013391154755795923
78-79	2.186552188722194E-4	4.680403603967507E-4	2.9075234509495123E-4	9.124423187532615E-4	0.0013722092384359282	0.0013923018801701322
80-81	2.2692865958630337E-4	4.8813300213095467E-4	2.931161852989752E-4	9.443541615075854E-4	0.0014100306817003121	0.001453761725474756
82-83	2.3520210030038736E-4	4.987702830490626E-4	3.0257154611507115E-4	9.597191228337414E-4	0.001451397885270732	0.0015317684522075479
84-85	2.4111170081044734E-4	5.176810046812546E-4	3.0729922652311914E-4	9.880852052820293E-4	0.001484491648127068	0.0015885006171041237
86-87	2.4229362091245934E-4	5.377736464154586E-4	3.143907471351911E-4	0.0010164512877303172	0.0015223130913914519	0.0016546881428167956
88-89	2.4702130132050733E-4	5.484109273335665E-4	3.191184275432391E-4	0.0010365439294645212	0.0015613164547578478	0.0017196937484274552
>>END_MODULE
