##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674202
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	56530142
Total Bases	5.6 Gbp
Sequences flagged as poor quality	0
Sequence length	62-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.40143773210405	37.0	36.0	37.0	31.0	37.0
2	35.07016725696532	37.0	35.0	37.0	30.0	37.0
3	35.23328517377508	37.0	36.0	37.0	31.0	37.0
4	35.259328147450965	37.0	36.0	37.0	31.0	37.0
5	35.26151807649802	37.0	36.0	37.0	31.0	37.0
6	35.073523148057895	37.0	36.0	37.0	30.0	37.0
7	35.2227186338927	37.0	36.0	37.0	31.0	37.0
8	35.22157787963809	37.0	36.0	37.0	31.0	37.0
9	35.15065610484403	37.0	36.0	37.0	30.0	37.0
10-11	35.20101346994671	37.0	36.0	37.0	30.5	37.0
12-13	35.18161538493924	37.0	36.0	37.0	30.5	37.0
14-15	35.2299039864432	37.0	36.0	37.0	31.0	37.0
16-17	35.178800780298765	37.0	36.0	37.0	30.5	37.0
18-19	35.190289943018364	37.0	36.0	37.0	31.0	37.0
20-21	35.10832923434015	37.0	36.0	37.0	30.5	37.0
22-23	35.10426916139712	37.0	36.0	37.0	30.5	37.0
24-25	35.172731053815504	37.0	36.0	37.0	30.5	37.0
26-27	35.16349130168469	37.0	36.0	37.0	30.0	37.0
28-29	35.186746948203314	37.0	36.0	37.0	31.0	37.0
30-31	35.1966950215692	37.0	36.0	37.0	31.0	37.0
32-33	35.17519978103009	37.0	36.0	37.0	30.5	37.0
34-35	35.043558080926104	37.0	36.0	37.0	30.0	37.0
36-37	35.15804134190924	37.0	36.0	37.0	30.5	37.0
38-39	35.07373227542927	37.0	35.5	37.0	30.0	37.0
40-41	35.12931816092024	37.0	36.0	37.0	30.0	37.0
42-43	35.16981465392392	37.0	36.0	37.0	30.0	37.0
44-45	35.15501204649371	37.0	36.0	37.0	30.0	37.0
46-47	35.08819440078534	37.0	36.0	37.0	30.0	37.0
48-49	35.1044893359723	37.0	35.5	37.0	30.0	37.0
50-51	35.13145649802189	37.0	36.0	37.0	30.0	37.0
52-53	35.082955859194556	37.0	35.5	37.0	30.0	37.0
54-55	35.047785807790824	37.0	35.0	37.0	30.0	37.0
56-57	35.09338698459311	37.0	35.0	37.0	30.0	37.0
58-59	34.963757732998445	37.0	35.0	37.0	30.0	37.0
60-61	35.046393992783536	37.0	35.0	37.0	30.0	37.0
62-63	35.063261835316865	37.0	35.0	37.0	30.0	37.0
64-65	35.017699805489606	37.0	35.0	37.0	30.0	37.0
66-67	35.053550955295705	37.0	35.0	37.0	30.0	37.0
68-69	35.004232733193305	37.0	35.0	37.0	30.0	37.0
70-71	35.015190412193675	37.0	35.0	37.0	30.0	37.0
72-73	35.02368363516315	37.0	35.0	37.0	30.0	37.0
74-75	35.004067184909	37.0	35.0	37.0	30.0	37.0
76-77	34.992314266238886	37.0	35.0	37.0	30.0	38.0
78-79	34.89060629309037	37.0	35.0	37.0	30.0	37.0
80-81	34.94678508687353	37.0	35.0	37.0	30.0	38.0
82-83	34.866120439848615	37.0	35.0	37.0	29.0	38.0
84-85	34.82579509241092	37.0	35.0	37.0	29.0	38.0
86-87	34.7731688743283	37.0	35.0	37.0	29.0	38.0
88-89	34.78194051007932	37.0	35.0	37.0	29.0	38.0
90-91	34.74142633809325	37.0	35.0	37.0	29.0	38.0
92-93	34.70095136404517	37.0	35.0	37.0	29.0	38.0
94-95	34.581490559303624	37.0	35.0	37.0	29.0	38.0
96-97	34.63737518954445	37.0	35.0	37.0	29.0	38.0
98-99	34.69319469092524	37.0	35.0	37.0	29.0	38.0
100	35.192184645747034	37.0	35.0	37.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	153.0
20	8165.0
21	44591.0
22	89305.0
23	142508.0
24	206006.0
25	279067.0
26	376799.0
27	509151.0
28	681664.0
29	906946.0
30	1195264.0
31	1588878.0
32	2167134.0
33	3147102.0
34	5170287.0
35	1.1598353E7
36	2.6911372E7
37	1507397.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.755395854819476	32.41362478847445	21.34605019049024	23.48492916621584
2	26.120329415612396	31.89419708703614	27.234809616325112	14.750663881026348
3	14.128995850126799	29.05276890038594	33.58155810507964	23.23667714440763
4	16.65339857355255	31.029558005055115	31.79007497782427	20.52696844356807
5	20.20308691746424	28.3765626444058	30.81505548598415	20.605294952145808
6	19.92066846975386	28.184225797373287	30.96659491453602	20.928510818336832
7	20.760033238569907	29.655346253388178	29.500177482355284	20.08444302568663
8	18.822706921766354	27.46976259227869	31.762318434474366	21.94521205148059
9	20.032723088220305	26.785908705027346	31.580624354154242	21.60074385259811
10-11	21.27889935067611	27.865694112034323	29.25174919716938	21.603657340120186
12-13	21.300152192128035	28.34194979593073	28.704481790670496	21.653416221270735
14-15	21.393134333382648	28.43329434995307	28.58971581557149	21.583855501092785
16-17	21.540619150983087	28.463504655717166	28.533064866130296	21.462811327169447
18-19	21.42447573393083	28.36049653783291	28.535311974183315	21.679715754052946
20-21	21.519703777325343	28.255291853752407	28.544384971814736	21.680619397107513
22-23	21.60197169281649	28.518709713288633	28.378831611596457	21.500486982298423
24-25	21.550711891566394	28.428173959141546	28.39358363460357	21.62753051468849
26-27	21.628150070917346	28.299149663446883	28.466129291012106	21.606570974623672
28-29	21.737347383871946	28.377431821353788	28.32377863905909	21.561442155715174
30-31	21.62493162228859	28.32607736057426	28.320325522042367	21.728665495094788
32-33	21.64017559993687	28.290659836816822	28.38474197511725	21.684422588129056
34-35	21.708020964229792	28.43606383456073	28.309896782902495	21.546018418306982
36-37	21.63821895706419	28.348038302054192	28.32703482782204	21.686707913059582
38-39	21.702527894634734	28.228022337155483	28.373496212930604	21.695953555279175
40-41	21.77093652312106	28.338505700002543	28.255055595424107	21.63550218145229
42-43	21.64925747833231	28.300114948767924	28.2907066644025	21.759920908497264
44-45	21.66911843970104	28.26713611012383	28.355751662799978	21.70799378737515
46-47	21.74314645655386	28.388001312708976	28.28576481774116	21.583087412996
48-49	21.66315137529607	28.2886414321591	28.29652518808209	21.751682004462744
50-51	21.720439299689108	28.20501429582109	28.324218468703272	21.75032793578653
52-53	21.759939539936774	28.322282633978702	28.268519054167434	21.64925877191709
54-55	21.658930768523927	28.293626214584002	28.277104123299495	21.770338893592577
56-57	21.691345062315825	28.252186451213824	28.35131829595684	21.70515019051351
58-59	21.765278428206948	28.330374044412977	28.308463347589502	21.595884179790577
60-61	21.690447150301964	28.280407721645023	28.274246344354893	21.754898783698124
62-63	21.73330244121484	28.20347065361237	28.3157590399542	21.747467865218585
64-65	21.781660974060003	28.343205428922165	28.233055355466206	21.642078241551623
66-67	21.67276198222079	28.306920987511734	28.272861645395224	21.747455384872246
68-69	21.68874374246551	28.244814349989966	28.341333382045097	21.725108525499426
70-71	21.790271376663274	28.35939962228825	28.23828088397994	21.612048117068543
72-73	21.685134095895073	28.282017015400434	28.255687104905313	21.777161783799176
74-75	21.72701340735293	28.21089215397128	28.311602550300353	21.75049188837544
76-77	21.76862415487857	28.353134829651093	28.25060113417617	21.627639881294165
78-79	21.66610498061226	28.281835973305046	28.30395280476053	21.748106241322162
80-81	21.714074561819146	28.217844726587188	28.352157548519102	21.715923163074564
82-83	21.79833722551484	28.3268825072065	28.2301749787988	21.644605288479866
84-85	21.70025609006553	28.254514671143365	28.258715581280992	21.786513657510113
86-87	21.74439554327765	28.218990742875942	28.29231650641615	21.744297207430257
88-89	21.791717338390075	28.331402905424873	28.242988602342738	21.63389115384231
90-91	21.708784921407148	28.285216083451083	28.265652988674606	21.740346006467163
92-93	21.754504931595047	28.18873279084231	28.324502686429025	21.73225959113362
94-95	21.82951046267302	28.320923357636918	28.208794931183633	21.640771248506425
96-97	21.714500620858924	28.289497434991205	28.23406670230965	21.76193524184022
98-99	21.745274610031785	28.198641536883944	28.310356201711347	21.745727651372924
100	22.007728110415584	27.957290816643095	28.081626257219135	21.953354815722186
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	9.0
2	3.0
3	3.5
4	5.0
5	6.0
6	8.5
7	18.5
8	56.5
9	112.5
10	186.0
11	378.5
12	745.5
13	1366.5
14	2376.0
15	3988.0
16	6662.0
17	11913.0
18	20138.5
19	31802.0
20	49569.5
21	74743.5
22	107759.5
23	152136.0
24	212228.5
25	286070.0
26	370610.5
27	463650.0
28	562989.0
29	672234.0
30	790655.0
31	915307.5
32	1043578.0
33	1184044.0
34	1343572.0
35	1502815.5
36	1666478.0
37	1855944.5
38	2043720.5
39	2206934.0
40	2356818.0
41	2492425.5
42	2606098.5
43	2679232.0
44	2714664.5
45	2703853.0
46	2644444.5
47	2532390.0
48	2375073.0
49	2209355.5
50	2030918.0
51	1836868.5
52	1634250.0
53	1435976.0
54	1243347.5
55	1054473.5
56	880916.5
57	731389.5
58	603526.5
59	490557.5
60	392916.0
61	313625.5
62	246898.0
63	189959.0
64	144682.5
65	109264.5
66	82564.5
67	62694.0
68	47217.5
69	34756.0
70	24857.0
71	17319.0
72	12037.0
73	8366.5
74	5642.0
75	3680.5
76	2345.0
77	1464.0
78	928.0
79	602.5
80	384.0
81	228.0
82	144.5
83	113.5
84	73.5
85	38.0
86	25.5
87	20.0
88	13.5
89	12.0
90	10.5
91	6.5
92	5.5
93	7.0
94	5.5
95	4.0
96	3.5
97	3.0
98	2.5
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04030416198140808
2	0.0021015337269097963
3	8.190320838040705E-4
4	0.059621290178255695
5	0.04783996473951896
6	0.3153591936846718
7	0.030925094792792134
8	0.002922334778497461
9	0.23898400962799635
10-11	0.07988216268765078
12-13	0.12776988955732677
14-15	8.78292504554473E-4
16-17	0.019036746803147955
18-19	0.01458602385962519
20-21	0.17240360018908144
22-23	0.22041515480360901
24-25	0.040705717668283944
26-27	0.038477702744847166
28-29	0.015562494076169135
30-31	6.91666403385295E-4
32-33	0.03152477487143054
34-35	0.27819229606746787
36-37	0.04879697631044337
38-39	0.20135629590316614
40-41	0.09107176840277528
42-43	3.1045384602076536E-4
44-45	0.014967236416989717
46-47	0.15042240651014108
48-49	0.12786187588207368
50-51	0.03307527513375077
52-53	0.06951689595968112
54-55	0.10665637457623935
56-57	0.03278516441724134
58-59	0.2514746911479543
60-61	0.015369676587757378
62-63	0.015413016434781079
64-65	0.07707304321070065
66-67	0.04567298082049682
68-69	0.061111647698024456
70-71	0.027142161508800982
72-73	0.059344457742858785
74-75	0.014433895821873183
76-77	0.01453826597289263
78-79	0.1853259918071636
80-81	0.0015195448314018133
82-83	0.046141021775931816
84-85	0.032150334749472924
86-87	0.1596406009685672
88-89	0.03170665494976025
90-91	0.03333462619591078
92-93	0.10741073478969757
94-95	0.12159294964533332
96-97	0.0796478608401298
98-99	0.01185140040376832
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62	1.0
63	0.0
64	0.0
65	1.0
66	0.0
67	0.0
68	0.0
69	6.0
70	2.0
71	0.0
72	1.0
73	1.0
74	1.0
75	2.0
76	3.0
77	4.0
78	6.0
79	14.0
80	28.0
81	69.0
82	91.0
83	111.0
84	126.0
85	120.0
86	152.0
87	161.0
88	200.0
89	432.0
90	605.0
91	865.0
92	1334.0
93	2412.0
94	4872.0
95	9734.0
96	22702.0
97	64563.0
98	257195.0
99	1817796.0
100	5.4346532E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.40278896731002
#Duplication Level	Percentage of total
1	60.49318153543093
2	10.332664709862884
3	6.8962195964691215
4	5.188507038835309
5	3.8122170652029377
6	2.7111075489272074
7	2.040398727276192
8	1.3634787377651116
9	1.0099480902677014
>10	5.534495371099276
>50	0.5061031643472857
>100	0.11167841451605567
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	8.844838917970523E-6	1.7689677835941045E-6	3.537935567188209E-6	1.7689677835941045E-6	2.1227613403129253E-5	3.184142010469388E-5
2	8.844838917970523E-6	3.537935567188209E-6	1.4151742268752836E-5	3.537935567188209E-6	2.8303484537505672E-5	4.599316237344672E-5
3	1.7689677835941046E-5	7.075871134376418E-6	1.7689677835941046E-5	5.306903350782313E-6	3.184142010469388E-5	4.953109794063493E-5
4	1.945864561953515E-5	1.0613806701564627E-5	1.945864561953515E-5	1.0613806701564627E-5	4.2455226806258507E-5	5.306903350782313E-5
5	1.945864561953515E-5	1.2382774485158732E-5	2.1227613403129253E-5	1.2382774485158732E-5	4.953109794063493E-5	5.8375936858605447E-5
6	1.945864561953515E-5	1.4151742268752836E-5	2.8303484537505672E-5	1.4151742268752836E-5	5.306903350782313E-5	6.191387242579366E-5
7	1.945864561953515E-5	1.592071005234694E-5	3.537935567188209E-5	1.7689677835941046E-5	5.6606969075011344E-5	6.368284020938776E-5
8	2.4765548970317464E-5	1.7689677835941046E-5	3.89172912390703E-5	2.1227613403129253E-5	6.191387242579366E-5	6.722077577657598E-5
9	3.0072452321099778E-5	3.0072452321099778E-5	4.4224194589852616E-5	2.4765548970317464E-5	6.722077577657598E-5	9.021735696329933E-5
10-11	3.0072452321099778E-5	3.2725903996490934E-5	4.8646614048837874E-5	3.361038788828799E-5	7.518113080274945E-5	9.81777119894728E-5
12-13	3.714832345547619E-5	3.89172912390703E-5	5.306903350782313E-5	4.0686259022664404E-5	8.491045361251701E-5	1.1409842204181974E-4
14-15	5.306903350782313E-5	4.0686259022664404E-5	6.10293885339966E-5	4.776213015704082E-5	8.933287307150228E-5	1.2028980928439911E-4
16-17	5.8375936858605447E-5	4.8646614048837874E-5	7.252767912735829E-5	5.395351739962019E-5	1.0879151869103742E-4	1.2913464820236964E-4
18-19	6.545180799298187E-5	6.456732410118482E-5	7.871906636993765E-5	6.898974356017007E-5	1.1409842204181974E-4	1.4151742268752836E-4
20-21	7.164319523556123E-5	7.075871134376418E-5	8.579493750431407E-5	7.518113080274945E-5	1.2205877706799322E-4	1.6362951998245468E-4
22-23	7.695009858634354E-5	8.048803415353176E-5	8.933287307150228E-5	8.137251804532881E-5	1.3267258376955783E-4	1.9723990787074265E-4
24-25	8.048803415353176E-5	9.110184085509639E-5	9.906219588126986E-5	9.021735696329933E-5	1.3797948712034015E-4	2.131606179230896E-4
26-27	8.667942139611112E-5	1.0436909923205217E-4	1.0525358312384922E-4	1.0436909923205217E-4	1.5124674549729596E-4	2.2819684408363947E-4
28-29	9.729322809767575E-5	1.1321393815002269E-4	1.1056048647463153E-4	1.1409842204181974E-4	1.671674555496429E-4	2.3969513467700116E-4
30-31	1.0525358312384922E-4	1.18520841500805E-4	1.2028980928439911E-4	1.2205877706799322E-4	1.8043471392659865E-4	2.4323307024418938E-4
32-33	1.1321393815002269E-4	1.247122287433844E-4	1.300191320941667E-4	1.2825016431057258E-4	1.9104852062816328E-4	2.635761997555216E-4
34-35	1.1763635760900795E-4	1.2736568041877552E-4	1.388639710121372E-4	1.3444155155315196E-4	1.9723990787074265E-4	2.7949690980786854E-4
36-37	1.2559671263518142E-4	1.317880998777608E-4	1.4947777771370183E-4	1.4063293879573133E-4	2.0696923068051022E-4	2.918796842930273E-4
38-39	1.35326035444949E-4	1.3621051933674605E-4	1.574381327398753E-4	1.5390019717268709E-4	2.1227613403129253E-4	3.113383299125624E-4
40-41	1.4328639047112246E-4	1.4593984214651364E-4	1.6362951998245468E-4	1.6362951998245468E-4	2.273123601918424E-4	3.3079697553209755E-4
42-43	1.4593984214651364E-4	1.5301571328089005E-4	1.7335884279222224E-4	1.6628297165784584E-4	2.467710058113776E-4	3.4848665336803856E-4
44-45	1.5390019717268709E-4	1.6186055219886058E-4	1.8397264949378687E-4	1.6893642333323696E-4	2.582692964047393E-4	3.6529184731218256E-4
46-47	1.6362951998245465E-4	1.671674555496429E-4	1.875105850609751E-4	1.707053911168311E-4	2.759589742406803E-4	3.865194607153118E-4
48-49	1.7335884279222224E-4	1.6805193944143993E-4	1.945864561953515E-4	1.7866574614300456E-4	2.839193292668538E-4	4.112850096856293E-4
50-51	1.795502300348016E-4	1.742433266840193E-4	2.0254681122152497E-4	1.8662610116917803E-4	3.086848782371713E-4	4.2720571973797625E-4
52-53	1.945864561953515E-4	1.8308816560198984E-4	2.0696923068051022E-4	1.8839506895277215E-4	3.352193949910828E-4	4.448953975739173E-4
54-55	2.0254681122152497E-4	1.875105850609751E-4	2.2200545684106013E-4	1.9812439176253972E-4	3.6794529898757374E-4	4.634695593016554E-4
56-57	2.113916501394955E-4	1.9104852062816328E-4	2.2554339240824834E-4	2.043157790051191E-4	3.767901379055443E-4	4.838126888129876E-4
58-59	2.299658118672336E-4	1.945864561953515E-4	2.3969513467700116E-4	2.0873819846410434E-4	3.9536429963328237E-4	5.06809269999711E-4
60-61	2.3704168300161E-4	1.945864561953515E-4	2.467710058113776E-4	2.1492958570668372E-4	4.2189881638719394E-4	5.24498947835652E-4
62-63	2.494244574867687E-4	2.043157790051191E-4	2.5119342527036285E-4	2.1758303738207485E-4	4.4224194589852613E-4	5.563403679403459E-4
64-65	2.529623930539569E-4	2.0696923068051022E-4	2.556158447293481E-4	2.2731236019184244E-4	4.57278172059076E-4	5.846438524778516E-4
66-67	2.6357619975552154E-4	2.1581406959848075E-4	2.582692964047393E-4	2.4234858635239234E-4	4.802747532457994E-4	6.067559497727778E-4
68-69	2.688831031063039E-4	2.2288994073285716E-4	2.662296514309128E-4	2.529623930539569E-4	4.970799471899433E-4	6.350594343102836E-4
70-71	2.7419000645708617E-4	2.2642787630004538E-4	2.7330552256528916E-4	2.618072319719275E-4	5.191920444848697E-4	6.598249832806011E-4
72-73	2.856882970504479E-4	2.2819684408363947E-4	2.7949690980786854E-4	2.7153655478169504E-4	5.413041417797961E-4	6.907819194934978E-4
74-75	2.954176198602154E-4	2.3085029575903066E-4	2.927641681848243E-4	2.768434581324774E-4	5.616472712911282E-4	7.190854040310035E-4
76-77	3.016090071027948E-4	2.3704168300161E-4	3.0603142656178005E-4	2.8038139369966554E-4	5.811059169106633E-4	7.580026952700737E-4
78-79	3.113383299125624E-4	2.467710058113776E-4	3.1576074937154767E-4	2.8745726483404203E-4	5.996800786384014E-4	7.942665348337529E-4
80-81	3.2106765272233E-4	2.5384687694575403E-4	3.201831688305329E-4	2.945331359684184E-4	6.217921759333277E-4	8.199165676958675E-4
82-83	3.272590399649093E-4	2.582692964047393E-4	3.3079697553209755E-4	3.007245232109978E-4	6.509801443626305E-4	8.46451084449779E-4
84-85	3.352193949910828E-4	2.688831031063039E-4	3.3787284666647393E-4	3.0691591045357717E-4	6.651318866313833E-4	8.791769884462699E-4
86-87	3.440642339090533E-4	2.7684345813247735E-4	3.467176855844445E-4	3.1399178158795355E-4	6.854750161427155E-4	9.340149897376871E-4
88-89	3.4760216947624155E-4	2.9011071650943316E-4	3.591004600696032E-4	3.2283662050592405E-4	7.084715973294389E-4	9.640874420587869E-4
>>END_MODULE
