##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674216
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	51703378
Total Bases	5.1 Gbp
Sequences flagged as poor quality	0
Sequence length	61-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.19531685144441	37.0	35.0	37.0	31.0	37.0
2	34.76866931209021	37.0	35.0	37.0	29.0	37.0
3	35.022223402888685	37.0	35.0	37.0	30.0	37.0
4	35.01632610929212	37.0	35.0	37.0	30.0	37.0
5	34.9936416533558	37.0	35.0	37.0	30.0	37.0
6	34.85565616234978	37.0	35.0	37.0	29.0	37.0
7	34.95138505263621	37.0	35.0	37.0	30.0	37.0
8	34.90431754768518	37.0	35.0	37.0	30.0	37.0
9	34.96056876593247	37.0	35.0	37.0	30.0	37.0
10-11	34.954042122740994	37.0	35.0	37.0	30.0	37.0
12-13	34.933361617107494	37.0	35.0	37.0	30.0	37.0
14-15	34.949914529762445	37.0	35.0	37.0	30.0	37.0
16-17	34.89991185875708	37.0	35.0	37.0	29.5	37.0
18-19	34.86312685410999	37.0	35.0	37.0	29.5	37.0
20-21	34.81498368443161	37.0	35.0	37.0	29.0	37.0
22-23	34.89875968065375	37.0	35.0	37.0	29.5	37.0
24-25	34.871687822795636	37.0	35.0	37.0	29.5	37.0
26-27	34.83881176970681	37.0	35.0	37.0	29.0	37.0
28-29	34.892016552574184	37.0	35.0	37.0	29.5	37.0
30-31	34.91153921316321	37.0	35.0	37.0	30.0	37.0
32-33	34.82457722588261	37.0	35.0	37.0	29.0	37.0
34-35	34.8127181844869	37.0	35.0	37.0	29.0	37.0
36-37	34.83187639113251	37.0	35.0	37.0	29.0	37.0
38-39	34.83202236805495	37.0	35.0	37.0	29.0	37.0
40-41	34.82932830810397	37.0	35.0	37.0	29.0	37.0
42-43	34.872969170408936	37.0	35.0	37.0	29.0	37.0
44-45	34.86766120967957	37.0	35.0	37.0	29.0	37.0
46-47	34.789830192526296	37.0	35.0	37.0	29.0	37.0
48-49	34.87499250049001	37.0	35.0	37.0	29.0	37.0
50-51	34.83366899160825	37.0	35.0	37.0	29.0	37.0
52-53	34.74827292715768	37.0	35.0	37.0	29.0	37.0
54-55	34.802446176727564	37.0	35.0	37.0	29.0	37.0
56-57	34.79640019845512	37.0	35.0	37.0	29.0	37.0
58-59	34.592681758627066	37.0	35.0	37.0	29.0	37.0
60-61	34.75972206303426	37.0	35.0	37.0	29.0	37.0
62-63	34.77511278959969	37.0	35.0	37.0	29.0	37.0
64-65	34.777431058511404	37.0	35.0	37.0	29.0	37.0
66-67	34.74366390913761	37.0	35.0	37.0	29.0	37.0
68-69	34.75369625340059	37.0	35.0	37.0	29.0	37.0
70-71	34.714329430907995	37.0	35.0	37.0	29.0	37.0
72-73	34.65792833885997	37.0	35.0	37.0	29.0	37.0
74-75	34.639307290236985	37.0	35.0	37.0	29.0	37.0
76-77	34.6742856917693	37.0	35.0	37.0	29.0	37.0
78-79	34.63291173109275	37.0	35.0	37.0	29.0	37.0
80-81	34.584085654247325	37.0	35.0	37.0	28.5	37.0
82-83	34.547849952731255	37.0	35.0	37.0	28.0	37.0
84-85	34.48207477845877	37.0	35.0	37.0	28.0	37.0
86-87	34.429441478882644	37.0	34.5	37.0	28.0	37.0
88-89	34.365373249710075	37.0	34.5	37.0	27.5	37.0
90-91	34.35477815895443	37.0	34.0	37.0	28.0	37.0
92-93	34.33075232019368	37.0	34.0	37.0	28.0	37.0
94-95	34.18511743663072	37.0	34.0	37.0	27.0	37.0
96-97	34.304737891325246	37.0	34.0	37.0	27.0	37.0
98-99	34.32238831831222	37.0	34.0	37.0	27.5	37.0
100	34.93692337984741	37.0	35.0	37.0	29.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	192.0
20	9532.0
21	50065.0
22	100023.0
23	161455.0
24	232290.0
25	315082.0
26	426763.0
27	576547.0
28	770239.0
29	1019182.0
30	1347234.0
31	1793683.0
32	2439980.0
33	3496000.0
34	5506777.0
35	1.09091E7
36	2.1454742E7
37	1094492.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	26.627594118115244	27.00814403085708	23.62841707618849	22.73584477483919
2	26.73933134990312	30.585371797207234	25.00586965542143	17.66942719746821
3	17.651145960255924	26.915060144747216	31.82824891241535	23.60554498258151
4	20.684030776717275	28.024008429532106	29.520864220172587	21.771096573578035
5	21.597613937761842	26.86244099449484	29.321656239626364	22.21828882811695
6	21.146636938834195	27.422074892124865	28.650741115948623	22.780547053092317
7	22.390281458321653	28.22137781949317	27.963294307702398	21.42504641448278
8	20.87627058842124	26.97068849087612	28.98719316291021	23.16584775779243
9	21.559959109469485	26.489775606382704	29.050840577670662	22.899424706477152
10-11	22.366478532970252	27.163558388688898	27.765136049428424	22.70482702891243
12-13	22.31648347042344	27.397108791284268	27.580788330428767	22.705619407863523
14-15	22.3088740461042	27.47682554556492	27.58023799689096	22.634062411439917
16-17	22.436489615098548	27.552568145635675	27.48843165722891	22.522510582036865
18-19	22.41128282292875	27.508515759762492	27.4348238078866	22.645377609422155
20-21	22.392954197623418	27.414244755149575	27.51712122857842	22.675679818648586
22-23	22.49699533081782	27.60822856517404	27.354632860640514	22.54014324336762
24-25	22.43856366027962	27.531947826524938	27.452061309451164	22.57742720374428
26-27	22.42904324402688	27.423831027163388	27.583988024836557	22.563137703973172
28-29	22.574186027106656	27.55773398631674	27.371437722688153	22.496642263888454
30-31	22.524661772317813	27.457251232397166	27.370939816250907	22.647147179034118
32-33	22.484778771390502	27.423713437893554	27.452900327721068	22.638607462994877
34-35	22.55406793125834	27.59775942318886	27.360243928894086	22.487928716658708
36-37	22.492015246250013	27.505927239278204	27.442862726294397	22.559194788177383
38-39	22.505678167549934	27.40152707785481	27.513238492173382	22.579556262421878
40-41	22.619317176370835	27.52022764609865	27.32783732099973	22.532617856530784
42-43	22.524956181521322	27.474151785108734	27.344674399209236	22.656217634160704
44-45	22.48798213286203	27.438143081992315	27.463214218112984	22.61066056703267
46-47	22.54825229672343	27.570741448268542	27.414003560891754	22.467002694116267
48-49	22.51368607844542	27.454634849910946	27.4691524096983	22.562526661945334
50-51	22.524988354946082	27.369132385657085	27.49068710221604	22.61519215718079
52-53	22.60091684914423	27.505470450399987	27.36030633572505	22.533306364730734
54-55	22.532334287932684	27.462340373770903	27.387563042835495	22.617762295460917
56-57	22.478619969655252	27.41611750795043	27.53034931928849	22.574913203105833
58-59	22.58582433749746	27.48754944153613	27.44272049214194	22.483905728824467
60-61	22.573270606030196	27.41129745772498	27.424926301685392	22.59050563455943
62-63	22.5361156903104	27.372179302499866	27.484387445974924	22.607317561214817
64-65	22.610889709259276	27.518752911131976	27.364470836289385	22.505886543319363
66-67	22.512071625109467	27.45680014961592	27.456720826015612	22.574407399259
68-69	22.489091096578427	27.38135169818004	27.569083177863945	22.560474027377587
70-71	22.621901459797748	27.4967054025479	27.409455900424938	22.47193723722941
72-73	22.571975288893338	27.416713269933506	27.4224210779018	22.588890363271354
74-75	22.54210184353567	27.37687269527531	27.494142629510293	22.58688283167872
76-77	22.609505037793035	27.48435529711175	27.411640654552293	22.494499010542924
78-79	22.553080998142804	27.422389800991738	27.464080783447592	22.560448417417874
80-81	22.52851024197789	27.347927585110902	27.56265068442601	22.5609114884852
82-83	22.664516095325613	27.445764057517042	27.387983658444227	22.50173618871312
84-85	22.61508156372372	27.403802278747474	27.390848655131034	22.590267502397772
86-87	22.563588166004575	27.37454464883057	27.487934492281234	22.573932692883616
88-89	22.656587928566115	27.482649923863807	27.39632896996359	22.464433177606494
90-91	22.622670651039943	27.392907133456628	27.434030278755078	22.55039193674835
92-93	22.598553406361766	27.341973517428496	27.51269585457987	22.546777221629867
94-95	22.678834357810256	27.518172163717797	27.338304920396396	22.46468855807555
96-97	22.598694231793715	27.64053668861991	27.304939559207032	22.45582952037934
98-99	22.564377050151112	27.48969903454436	27.464338020855106	22.481585894449427
100	23.102942062671698	27.012416726392164	27.11125074517753	22.77339046575861
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	4.0
2	5.5
3	8.0
4	10.5
5	20.0
6	39.5
7	96.0
8	190.5
9	373.5
10	744.0
11	1479.5
12	2670.0
13	4219.5
14	6256.5
15	9449.0
16	13801.5
17	19807.0
18	28757.5
19	40755.0
20	57174.0
21	80075.0
22	111244.0
23	152926.0
24	204381.5
25	265960.5
26	336219.0
27	409969.5
28	484542.5
29	563746.0
30	650964.5
31	740046.0
32	829103.0
33	913338.5
34	987789.5
35	1062253.0
36	1149097.5
37	1244315.0
38	1338576.5
39	1436964.0
40	1547392.5
41	1677173.5
42	1815959.0
43	1947891.0
44	2074313.5
45	2198852.0
46	2312560.0
47	2401406.5
48	2443209.5
49	2422026.5
50	2348350.0
51	2217221.0
52	2036880.5
53	1824640.5
54	1587532.0
55	1341591.0
56	1110058.0
57	909805.5
58	741470.5
59	604066.5
60	499175.5
61	421118.0
62	358636.5
63	308935.5
64	267755.5
65	231185.5
66	198182.5
67	168285.0
68	139597.0
69	112427.0
70	88010.0
71	66775.0
72	49180.0
73	35074.5
74	24044.5
75	16084.0
76	10699.5
77	6879.0
78	4376.0
79	2751.0
80	1666.0
81	974.5
82	577.0
83	397.0
84	275.5
85	171.5
86	113.5
87	77.5
88	52.5
89	43.0
90	32.5
91	24.0
92	18.5
93	11.0
94	8.0
95	9.0
96	7.5
97	4.0
98	3.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02752624789815474
2	0.026694580768010942
3	5.338142509760194E-4
4	0.05582033730948875
5	0.0015221442591236494
6	0.188417089498485
7	0.07336271142670794
8	0.14569647654356355
9	0.053853347841218424
10-11	0.036642673521254256
12-13	0.05215036433402862
14-15	0.0023015904299328372
16-17	0.039899714096049976
18-19	0.1370780841437478
20-21	0.24743450998501493
22-23	0.05571009306200458
24-25	0.16106877968398892
26-27	0.19452307352142445
28-29	0.07419051033764178
30-31	9.361090488130196E-4
32-33	0.2227078857400768
34-35	0.1702180851703732
36-37	0.1226244830656906
38-39	0.07977138360282766
40-41	0.14606782558772077
42-43	0.04583259530934323
44-45	0.0163877106830428
46-47	0.21135853057802143
48-49	9.167679527631637E-4
50-51	0.07700270570329079
52-53	0.21284586086425533
54-55	0.0773518124869907
56-57	0.046908927304517706
58-59	0.4921941463863348
60-61	0.0010318474742598055
62-63	0.026541787135911588
64-65	0.02887916035961522
66-67	0.031637202146838746
68-69	0.0010260453637381101
70-71	0.014238920144433943
72-73	0.20764419155560107
74-75	0.15660791641543365
76-77	0.01160564421324747
78-79	0.0444575736487161
80-81	0.07428183443985983
82-83	0.045249100699509125
84-85	0.12396292517959408
86-87	0.13572279309289828
88-89	0.09094745986326798
90-91	0.030442966586191764
92-93	0.060008045306741586
94-95	0.13873494935190517
96-97	0.0017869385882544333
98-99	0.042290040004914806
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
61	1.0
62	2.0
63	2.0
64	0.0
65	1.0
66	2.0
67	2.0
68	2.0
69	6.0
70	2.0
71	4.0
72	11.0
73	13.0
74	10.0
75	17.0
76	24.0
77	30.0
78	45.0
79	52.0
80	134.0
81	207.0
82	252.0
83	243.0
84	328.0
85	382.0
86	536.0
87	523.0
88	735.0
89	911.0
90	1177.0
91	1660.0
92	2658.0
93	4101.0
94	7397.0
95	13429.0
96	31785.0
97	81892.0
98	309985.0
99	1976356.0
100	4.9268461E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.55470517602869
#Duplication Level	Percentage of total
1	29.939366066853196
2	10.850463894290646
3	8.853934082823113
4	8.17390524968797
5	7.149883346997451
6	5.997862490763028
7	5.247976336897679
8	4.176010198313464
9	3.301530848044161
>10	15.532667963418568
>50	0.5008614529510913
>100	0.27396343880863194
>500	0.0015746301510345595
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	5.802328814956732E-6	9.670548024927888E-6	2.1275205654841354E-5
2	0.0	1.9341096049855774E-6	0.0	7.73643841994231E-6	4.0616301704697126E-5	2.320931525982693E-5
3	0.0	5.802328814956732E-6	0.0	9.670548024927888E-6	5.415506893959617E-5	3.094575367976924E-5
4	0.0	5.802328814956732E-6	0.0	9.670548024927888E-6	7.349616498945194E-5	3.4813972889740395E-5
5	3.868219209971155E-6	5.802328814956732E-6	1.9341096049855774E-6	1.1604657629913464E-5	7.929849380440868E-5	4.0616301704697126E-5
6	3.868219209971155E-6	7.73643841994231E-6	5.802328814956732E-6	1.3538767234899043E-5	9.47713706442933E-5	4.641863051965386E-5
7	3.868219209971155E-6	7.73643841994231E-6	7.73643841994231E-6	1.3538767234899043E-5	1.0831013787919234E-4	5.2220959334610596E-5
8	7.73643841994231E-6	9.670548024927888E-6	7.73643841994231E-6	1.3538767234899043E-5	1.1798068590412024E-4	5.415506893959617E-5
9	7.73643841994231E-6	9.670548024927888E-6	7.73643841994231E-6	1.7406986444870198E-5	1.2184890511409139E-4	7.156205538446637E-5
10-11	1.2571712432406254E-5	1.1604657629913466E-5	1.0637602827420675E-5	1.7406986444870198E-5	1.547287683988462E-4	8.316671301437983E-5
12-13	1.643993164237741E-5	1.3538767234899043E-5	1.4505822037391832E-5	1.8374041247362985E-5	1.7697102885618035E-4	1.034748638667284E-4
14-15	1.9341096049855776E-5	1.3538767234899043E-5	2.0308150852348567E-5	2.5143424864812507E-5	2.0114739891850006E-4	1.1894774070661301E-4
16-17	2.320931525982693E-5	1.3538767234899043E-5	2.224226045733414E-5	2.7077534469798085E-5	2.3015904299328373E-4	1.3635472715148322E-4
18-19	2.9011644074783664E-5	1.3538767234899043E-5	2.6110479667305298E-5	2.9011644074783664E-5	2.620718514755458E-4	1.566628780038318E-4
20-21	3.094575367976924E-5	1.3538767234899043E-5	3.094575367976924E-5	3.4813972889740395E-5	2.93017605155315E-4	1.7600397405368758E-4
22-23	3.3846918087247604E-5	1.3538767234899043E-5	3.674808249472597E-5	3.868219209971155E-5	3.3846918087247606E-4	1.8567452207861546E-4
24-25	4.255041130968271E-5	1.3538767234899043E-5	4.0616301704697126E-5	4.448452091466828E-5	3.790854825771732E-4	2.0114739891850006E-4
26-27	4.448452091466828E-5	1.4505822037391832E-5	4.158335650718992E-5	4.448452091466828E-5	4.051959622444785E-4	2.320931525982693E-4
28-29	4.835274012463944E-5	1.547287683988462E-5	4.255041130968271E-5	4.641863051965386E-5	4.284052775043054E-4	2.6303890627803856E-4
30-31	5.415506893959617E-5	1.547287683988462E-5	4.448452091466828E-5	5.1253904532117805E-5	4.564498667765963E-4	2.93017605155315E-4
32-33	5.802328814956733E-5	1.7406986444870198E-5	4.448452091466828E-5	5.2220959334610596E-5	4.883626752588584E-4	3.2009513962511307E-4
34-35	5.899034295206012E-5	1.8374041247362985E-5	4.448452091466828E-5	5.318801413710339E-5	5.164072645311491E-4	3.413703452799544E-4
36-37	6.0924452557045686E-5	2.0308150852348567E-5	4.5451575717161074E-5	5.415506893959617E-5	5.386495249884833E-4	3.7328315376221647E-4
38-39	6.769383617449522E-5	2.1275205654841354E-5	5.415506893959617E-5	5.6089178544581746E-5	5.67661169063267E-4	4.051959622444785E-4
40-41	7.059500058197358E-5	2.1275205654841354E-5	5.899034295206011E-5	5.802328814956733E-5	5.966728131380507E-4	4.35174661121755E-4
42-43	7.349616498945194E-5	2.1275205654841354E-5	6.189150735953848E-5	6.382561696452406E-5	6.160139091879065E-4	4.6225219559155306E-4
44-45	7.73643841994231E-5	2.320931525982693E-5	6.189150735953848E-5	6.575972656950964E-5	6.488937724726612E-4	4.970661684812934E-4
46-47	8.413376781687263E-5	2.320931525982693E-5	6.285856216203127E-5	6.575972656950964E-5	6.788724713499377E-4	5.396165797909761E-4
48-49	8.703493222435098E-5	2.5143424864812507E-5	6.575972656950964E-5	6.769383617449521E-5	7.117523346346925E-4	5.724964430757309E-4
50-51	9.090315143432215E-5	2.5143424864812507E-5	6.769383617449521E-5	6.769383617449521E-5	7.552698007468679E-4	6.005410323480218E-4
52-53	9.090315143432215E-5	2.7077534469798085E-5	6.769383617449521E-5	6.962794577948079E-5	7.862155544266372E-4	6.353550052377623E-4
54-55	9.090315143432215E-5	2.9011644074783664E-5	6.8660890976988E-5	7.059500058197358E-5	8.065237052789858E-4	6.924112385848368E-4
56-57	9.283726103930772E-5	3.094575367976924E-5	7.252911018695916E-5	7.156205538446637E-5	8.365024041562623E-4	7.262581566720844E-4
58-59	9.47713706442933E-5	3.384691808724761E-5	7.83314390019159E-5	7.156205538446637E-5	8.65514048231046E-4	7.591380199568393E-4
60-61	9.767253505177167E-5	3.578102769223318E-5	8.123260340939427E-5	7.156205538446637E-5	9.022621307257719E-4	8.007213764640291E-4
62-63	1.0154075426174282E-4	3.674808249472597E-5	8.316671301437983E-5	7.446321979194473E-5	9.457795968379474E-4	8.539093906011325E-4
64-65	1.0540897347171397E-4	3.964924690220434E-5	8.413376781687263E-5	7.543027459443752E-5	9.747912409127311E-4	8.945256923058296E-4
66-67	1.1024424748417791E-4	4.255041130968271E-5	8.896904182933657E-5	7.543027459443752E-5	0.0010163745974199208	9.399772680229907E-4
68-69	1.1411246669414907E-4	4.255041130968271E-5	8.896904182933657E-5	7.543027459443752E-5	0.0010502215155071687	9.912311725551084E-4
70-71	1.1411246669414907E-4	4.255041130968271E-5	8.896904182933657E-5	7.543027459443752E-5	0.0010927719268168513	0.001040550967482241
72-73	1.1604657629913465E-4	4.255041130968271E-5	9.090315143432215E-5	7.83314390019159E-5	0.001140157612138998	0.0010879366528043872
74-75	1.1798068590412024E-4	4.255041130968271E-5	9.283726103930772E-5	8.026554860690146E-5	0.0011885103522636374	0.001144992886151462
76-77	1.2184890511409139E-4	4.255041130968271E-5	9.38043158418005E-5	8.219965821188704E-5	0.001235896037585784	0.0012049502839060148
78-79	1.2378301471907695E-4	4.351746611217549E-5	9.670548024927888E-5	8.606787742185821E-5	0.0012987545997478153	0.0012581382980431182
80-81	1.286182887315409E-4	4.5451575717161074E-5	9.960664465675725E-5	8.896904182933657E-5	0.001341305011057498	0.0013219639150076424
82-83	1.3248650794151206E-4	4.641863051965386E-5	1.0057369945925003E-4	8.896904182933657E-5	0.0013809542579597024	0.0013925589155896157
84-85	1.3345356274400486E-4	4.641863051965386E-5	1.034748638667284E-4	8.993609663182936E-5	0.0014409116557142553	0.0014776597382089812
86-87	1.3345356274400486E-4	4.9319794927132223E-5	1.0540897347171397E-4	9.187020623681492E-5	0.0014902314506413873	0.0015579252868158826
88-89	1.3442061754649764E-4	5.318801413710339E-5	1.0831013787919234E-4	9.38043158418005E-5	0.0015530900128034187	0.00163335556141032
>>END_MODULE
