##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674217
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	50743944
Total Bases	5 Gbp
Sequences flagged as poor quality	0
Sequence length	61-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.81281054937314	37.0	35.0	37.0	29.0	37.0
2	34.22295637879468	37.0	34.0	37.0	27.0	37.0
3	34.47771513384927	37.0	34.0	37.0	28.0	37.0
4	34.489874831171974	37.0	34.0	37.0	28.0	37.0
5	34.43944883353962	37.0	34.0	37.0	28.0	37.0
6	34.31850190832624	37.0	34.0	37.0	28.0	37.0
7	34.4307230435222	37.0	34.0	37.0	28.0	37.0
8	34.37063699660397	37.0	34.0	37.0	28.0	37.0
9	34.40587793097044	37.0	34.0	37.0	28.0	37.0
10-11	34.37453668362869	37.0	34.0	37.0	28.0	37.0
12-13	34.345823848457655	37.0	34.0	37.0	28.0	37.0
14-15	34.31968824693642	37.0	34.0	37.0	27.5	37.0
16-17	34.27096692562959	37.0	34.0	37.0	27.0	37.0
18-19	34.25031117999026	37.0	34.0	37.0	27.0	37.0
20-21	34.182068849437485	37.0	34.0	37.0	27.0	37.0
22-23	34.187509134094896	37.0	34.0	37.0	27.0	37.0
24-25	34.154302678956135	37.0	34.0	37.0	27.0	37.0
26-27	34.130099495222524	37.0	34.0	37.0	27.0	37.0
28-29	34.14351293821387	37.0	34.0	37.0	27.0	37.0
30-31	34.1201437160659	37.0	34.0	37.0	27.0	37.0
32-33	34.05420205414069	37.0	34.0	37.0	27.0	37.0
34-35	34.02536563772024	37.0	34.0	37.0	26.5	37.0
36-37	33.99686600040391	37.0	34.0	37.0	26.5	37.0
38-39	33.96246433860166	37.0	34.0	37.0	26.0	37.0
40-41	33.96689779375446	37.0	34.0	37.0	26.0	37.0
42-43	33.97181784884518	37.0	34.0	37.0	26.0	37.0
44-45	33.926974714460506	37.0	34.0	37.0	26.0	37.0
46-47	33.802026947294436	37.0	33.5	37.0	26.0	37.0
48-49	33.87360292688325	37.0	33.0	37.0	26.0	37.0
50-51	33.81600146216463	37.0	33.0	37.0	26.0	37.0
52-53	33.71144890708535	36.5	33.0	37.0	25.5	37.0
54-55	33.7251991390342	36.5	33.0	37.0	25.5	37.0
56-57	33.67302677537245	36.0	33.0	37.0	25.0	37.0
58-59	33.5008256847359	36.0	33.0	37.0	25.0	37.0
60-61	33.58520439404552	36.0	33.0	37.0	25.0	37.0
62-63	33.51347417956858	36.0	33.0	37.0	25.0	37.0
64-65	33.548704783657506	36.0	33.0	37.0	25.0	37.0
66-67	33.471941693070264	36.0	33.0	37.0	25.0	37.0
68-69	33.440650026172726	36.0	33.0	37.0	24.5	37.0
70-71	33.38465787561956	36.0	33.0	37.0	24.0	37.0
72-73	33.31919227314721	36.0	33.0	37.0	24.0	37.0
74-75	33.20473787283893	36.0	33.0	37.0	24.0	37.0
76-77	33.24507856986184	36.0	33.0	37.0	24.0	37.0
78-79	33.18655365003353	36.0	32.5	37.0	23.5	37.0
80-81	33.06996713145924	36.0	32.0	37.0	23.0	37.0
82-83	33.01253432272806	36.0	32.0	37.0	23.0	37.0
84-85	32.94404672692235	36.0	32.0	37.0	23.0	37.0
86-87	32.86042677949082	36.0	32.0	37.0	22.5	37.0
88-89	32.73209790031917	36.0	32.0	37.0	22.0	37.0
90-91	32.71461717393021	36.0	32.0	37.0	22.0	37.0
92-93	32.68645681755456	36.0	32.0	37.0	22.0	37.0
94-95	32.52004670188906	36.0	31.0	37.0	21.0	37.0
96-97	32.62021452466566	36.0	31.5	37.0	21.5	37.0
98-99	32.833642625998046	36.0	31.5	37.0	22.5	37.0
100	34.249384216242234	36.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	411.0
20	19938.0
21	125513.0
22	306068.0
23	477362.0
24	604948.0
25	726690.0
26	879263.0
27	1071180.0
28	1313530.0
29	1621370.0
30	2016776.0
31	2536106.0
32	3276817.0
33	4422655.0
34	6493833.0
35	1.0863856E7
36	1.3590321E7
37	397306.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	27.084060948312498	30.64175498259273	18.36901354498709	23.905170524107685
2	25.338240679904782	28.9758213135217	27.484283586688385	18.20165441988513
3	19.53871746127696	27.540639549044883	29.67529452071168	23.245348468966476
4	21.48244301215045	27.258676844060336	27.98546548885571	23.273414654933507
5	22.23564945990277	27.472488275138375	27.61562988535901	22.676232379599846
6	21.998529856808968	26.687320698870458	28.114203950502088	23.199945493818486
7	23.4401962362492	27.36479696918861	26.861059397896593	22.3339473966656
8	21.415429575278775	26.715262363564634	28.560368703299204	23.30893935785739
9	21.97124202993187	26.02691743916632	28.747072447179068	23.254768083722745
10-11	22.883393944736774	26.836324845371283	27.364414934350695	22.915866275541244
12-13	22.81381307510512	26.855820536818086	27.193314276789167	23.13705211128763
14-15	22.672156470243564	27.04407144333786	27.354857404323297	22.928914682095282
16-17	23.010814507856196	27.04908415505336	27.137112841269378	22.802988495821065
18-19	22.925714427277732	26.77507478457542	27.137105691046003	23.162105097100838
20-21	22.815340991216225	26.874605443503093	27.284885476058225	23.025168089222454
22-23	23.092607490296256	27.069810538534966	26.9437382235932	22.89384374757558
24-25	23.019988644938405	26.817295179797373	27.02730470202527	23.135411473238953
26-27	22.887207317836356	26.887680617960342	27.237579564161145	22.987532500042153
28-29	23.18266625600041	26.96541453878037	26.913840708852394	22.93807849636683
30-31	23.11935849142897	26.717105278721178	26.94918532022991	23.214350909619945
32-33	22.970504810865965	26.84594382989836	27.114158255431743	23.069393103803932
34-35	23.230268869771074	26.96342990891362	26.844329382604414	22.961971838710888
36-37	23.170000865820846	26.71159369611424	26.913707640109635	23.20469779795528
38-39	23.03272631794771	26.79025679380402	27.09795411286938	23.079062775378894
40-41	23.286488033720488	26.874030296281287	26.823393144839535	23.01608852515869
42-43	23.19948414503551	26.65918424248818	26.857273226976737	23.284058385499574
44-45	23.055759099494853	26.76295336887136	27.069435780536473	23.11185175109732
46-47	23.309511704390403	26.837011965062572	26.831206858631596	23.02226947191543
48-49	23.243873593126555	26.624349773317345	26.874762131416023	23.25701450214007
50-51	23.111329637398075	26.72288346754501	27.02675282676978	23.139034068287135
52-53	23.354606533548896	26.798456960915473	26.78865580576058	23.058280699775047
54-55	23.272124701566916	26.607924328804145	26.820853037319964	23.299097932308975
56-57	23.135994311873525	26.694968904689766	27.03961423222757	23.129422551209142
58-59	23.38187294913466	26.765175789361624	26.79951531990793	23.05343594159579
60-61	23.324555794932614	26.55336085447839	26.836859621719512	23.285223728869486
62-63	23.182428463473308	26.640143239047408	27.014185747240067	23.163242550239215
64-65	23.4214413765266	26.756925729790797	26.755434553087294	23.066198340595303
66-67	23.343950167373006	26.54976141125185	26.811241602064495	23.29504681931065
68-69	23.197493497578492	26.64045755224936	27.011987610421972	23.150061339750177
70-71	23.46140845014901	26.72656163767956	26.763391097305224	23.04863881486621
72-73	23.393068393019647	26.53037618892144	26.774904947858285	23.30165047020063
74-75	23.274688133406592	26.60949754995	26.942463340077822	23.173350976565583
76-77	23.498352646484648	26.71095981690507	26.71583125004749	23.074856286562788
78-79	23.42087298307353	26.502628433101044	26.792184094847954	23.284314488977472
80-81	23.306223961062337	26.574653562550065	26.947995425046926	23.17112705134067
82-83	23.565614808262563	26.686606348807807	26.679567770151174	23.06821107277846
84-85	23.49156451180039	26.478061990341434	26.72478612550059	23.305587372357582
86-87	23.37292499583202	26.57300835452278	26.88014740827952	23.173919241365677
88-89	23.640763874254382	26.662563898602343	26.604806528787993	23.09186569835528
90-91	23.565660046031923	26.44243233456583	26.680980097712737	23.31092752168951
92-93	23.43953246005638	26.54432805136999	26.842359528831274	23.17377995974235
94-95	23.69496482978464	26.6280611557639	26.570707832398803	23.106266182052657
96-97	23.614096772338165	26.479104694897863	26.607507323021856	23.299291209742115
98-99	23.40961501533473	26.53310184515002	26.83576322835196	23.22151991116329
100	24.119250010593742	25.82836794148442	26.340042965121608	23.71233908280023
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	27.5
2	23.0
3	41.0
4	60.5
5	85.0
6	140.0
7	238.0
8	437.5
9	847.5
10	1538.5
11	2881.0
12	5020.0
13	8375.5
14	13324.0
15	20170.0
16	29785.0
17	42808.5
18	59452.5
19	80934.5
20	106872.0
21	136432.5
22	171408.5
23	212550.5
24	258887.0
25	310617.5
26	368333.5
27	429677.0
28	494351.5
29	562963.5
30	634014.5
31	708569.0
32	788595.0
33	869428.0
34	950200.0
35	1029105.5
36	1107086.0
37	1188194.0
38	1266513.5
39	1340121.0
40	1416542.5
41	1492093.0
42	1564579.5
43	1638935.0
44	1707645.5
45	1765226.5
46	1808929.0
47	1844772.5
48	1865573.5
49	1855259.0
50	1815796.0
51	1755242.5
52	1673177.0
53	1572134.5
54	1464908.0
55	1355515.5
56	1249498.0
57	1151971.5
58	1060929.0
59	976809.5
60	899363.5
61	824868.0
62	752046.0
63	680405.0
64	607151.5
65	532765.0
66	461102.0
67	393003.5
68	327930.5
69	266915.5
70	212128.0
71	164892.0
72	125575.5
73	93681.5
74	67763.5
75	47764.0
76	33100.5
77	22563.0
78	15146.5
79	10004.0
80	6479.0
81	4148.0
82	2667.0
83	1734.5
84	1127.0
85	763.0
86	498.5
87	319.5
88	218.5
89	138.0
90	87.0
91	54.0
92	45.0
93	39.5
94	31.0
95	21.0
96	12.5
97	12.5
98	9.5
99	4.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.020232956271589768
2	0.02762300068753032
3	0.006473678908363922
4	0.041003907776660006
5	9.616911133277303E-4
6	0.15397108273649365
7	0.07914639035546785
8	0.09289187296911726
9	0.034045441954610386
10-11	0.03180872184471904
12-13	0.040076703537273335
14-15	0.026666236270479884
16-17	0.04752094161226412
18-19	0.088324825520066
20-21	0.16440089875552438
22-23	0.09623414372363331
24-25	0.16150794270149754
26-27	0.1295533906469706
28-29	0.0519490562262957
30-31	0.03402179381247938
32-33	0.1481073288272587
34-35	0.11384609757570284
36-37	0.08003713704240253
38-39	0.11672722955866417
40-41	0.10230580421577005
42-43	0.031002714333753796
44-45	0.01624430296549279
46-47	0.16090885643417863
48-49	0.0032319127579046674
50-51	0.05207517965099441
52-53	0.14059904370066306
54-55	0.056849148343692014
56-57	0.0381001129908231
58-59	0.3284441193613173
60-61	0.023255977107337185
62-63	0.037890236476105135
64-65	0.024032425151041426
66-67	0.028148189284241412
68-69	0.014307128385814697
70-71	0.01719713195627461
72-73	0.14343885220934585
74-75	0.19435630087444766
76-77	0.018420931901495005
78-79	0.04340326488839437
80-81	0.06826352073973342
82-83	0.04552793585472997
84-85	0.097246506532667
86-87	0.10954118091853089
88-89	0.08476428053852185
90-91	0.047695469237880796
92-93	0.06104139353359997
94-95	0.11948228183705627
96-97	0.02936472512583336
98-99	0.054572404747909545
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
61	1.0
62	0.0
63	0.0
64	1.0
65	2.0
66	2.0
67	2.0
68	1.0
69	6.0
70	5.0
71	1.0
72	1.0
73	2.0
74	6.0
75	5.0
76	10.0
77	11.0
78	19.0
79	38.0
80	75.0
81	116.0
82	140.0
83	180.0
84	218.0
85	305.0
86	473.0
87	561.0
88	921.0
89	1286.0
90	1968.0
91	3342.0
92	6195.0
93	11260.0
94	22730.0
95	47467.0
96	110562.0
97	270718.0
98	828223.0
99	3584550.0
100	4.5852541E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.94548213077486
#Duplication Level	Percentage of total
1	77.69176768529874
2	11.33570926178308
3	4.392765820403329
4	2.0867925886659804
5	1.1437508086591779
6	0.5945332263945345
7	0.449355092177367
8	0.4397351283211891
9	0.33642141166103307
>10	1.5199001934682232
>50	0.002116270015346262
>100	0.00124547938254231
>500	0.0
>1k	0.005907033769443737
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.97067851091748E-6	0.0	1.1824071065504881E-5	3.94135702183496E-6	1.7736106598257322E-5	1.3006478172055368E-4
2	5.912035532752441E-6	7.88271404366992E-6	1.379474957642236E-5	9.8533925545874E-6	6.503239086027684E-5	1.5568360236248093E-4
3	5.912035532752441E-6	1.1824071065504881E-5	1.576542808733984E-5	1.576542808733984E-5	8.079781894761669E-5	1.576542808733984E-4
4	9.8533925545874E-6	1.97067851091748E-5	1.576542808733984E-5	2.167746362009228E-5	1.0444596107862645E-4	1.7341970896073825E-4
5	9.8533925545874E-6	2.167746362009228E-5	1.7736106598257322E-5	2.561882064192724E-5	1.1627003214413133E-4	1.8130242300440816E-4
6	1.379474957642236E-5	2.3648142131009762E-5	1.97067851091748E-5	3.94135702183496E-5	1.3400613874238864E-4	1.872144585371606E-4
7	1.379474957642236E-5	2.758949915284472E-5	2.167746362009228E-5	4.7296284262019525E-5	1.4583020980789353E-4	2.0297988662450046E-4
8	1.576542808733984E-5	3.153085617467968E-5	2.167746362009228E-5	6.109103383844188E-5	1.6159563789523338E-4	2.088919221572529E-4
9	2.561882064192724E-5	3.350153468559716E-5	2.167746362009228E-5	6.700306937119432E-5	1.675076734279858E-4	2.1874531471184028E-4
10-11	2.9560177663762203E-5	4.0398909473808345E-5	2.660415989738598E-5	7.192976564848803E-5	1.9608251183628926E-4	2.3056938577734518E-4
12-13	3.94135702183496E-5	5.4193659050230704E-5	3.054551691922094E-5	8.473917596945165E-5	2.2268667173367524E-4	2.4436413535376753E-4
14-15	4.236958798472582E-5	5.9120355327524406E-5	3.645755245197338E-5	9.55779077794978E-5	2.3648142131009762E-4	2.571735456747312E-4
16-17	4.631094500656078E-5	6.40470516048181E-5	3.94135702183496E-5	1.0937265735592015E-4	2.532321886528962E-4	2.72938973762071E-4
18-19	5.2222980539313226E-5	7.587112267032299E-5	4.9266962772937E-5	1.3203546023147117E-4	2.7392431301752974E-4	2.985577944039983E-4
20-21	6.109103383844188E-5	8.37538367139929E-5	6.0105694582983145E-5	1.4484487055243478E-4	2.896897411048696E-4	3.291033113232192E-4
22-23	6.601773011573559E-5	9.459256852403905E-5	6.109103383844188E-5	1.576542808733984E-4	3.143232224913381E-4	3.4782475717693524E-4
24-25	6.995908713757054E-5	1.0050460405679148E-4	6.109103383844188E-5	1.793317444934907E-4	3.3797136462234784E-4	3.763995955852387E-4
26-27	7.39004441594055E-5	1.1528469288867259E-4	6.207637309390063E-5	1.8524378002624312E-4	3.6950222079702754E-4	4.030037554826247E-4
28-29	8.473917596945165E-5	1.2809410320963621E-4	6.700306937119433E-5	1.9509717258083052E-4	4.039890947380834E-4	4.414319864455156E-4
30-31	9.459256852403905E-5	1.3400613874238866E-4	7.488578341486424E-5	2.0790658290179416E-4	4.20739862080882E-4	4.719775033647365E-4
32-33	9.951926480133275E-5	1.4977156682972849E-4	8.572451522491039E-5	2.2071599322275777E-4	4.463586827228093E-4	5.035083595394162E-4
34-35	1.0838731810046141E-4	1.6061029863977463E-4	9.262189001312156E-5	2.2761336801096897E-4	4.8084555666386516E-4	5.251858231595085E-4
36-37	1.1528469288867259E-4	1.7144903044982078E-4	1.0148994331225023E-4	2.3648142131009762E-4	5.094203950721686E-4	5.665700718887755E-4
38-39	1.2119672842142504E-4	1.8425844077078437E-4	1.1035799661137889E-4	2.473201531201437E-4	5.429219297577658E-4	6.020422850852901E-4
40-41	1.3105012097601242E-4	1.9509717258083052E-4	1.1627003214413133E-4	2.6407092046294234E-4	5.744527859324455E-4	6.345584805154286E-4
42-43	1.3597681725330613E-4	2.069212436463354E-4	1.231674069323425E-4	2.778656700393647E-4	6.237197487053824E-4	6.729867114783195E-4
44-45	1.4188885278605857E-4	2.2071599322275777E-4	1.2809410320963621E-4	2.8377770557211715E-4	6.74957389989237E-4	7.24224352762174E-4
46-47	1.4583020980789353E-4	2.2761336801096897E-4	1.2809410320963621E-4	2.936310981267045E-4	7.143709602075866E-4	7.853153866006159E-4
48-49	1.546982631070222E-4	2.384520998210151E-4	1.2907944246509493E-4	3.0446982993675065E-4	7.488578341486424E-4	8.355676886290116E-4
50-51	1.6258097715069213E-4	2.522468493974375E-4	1.379474957642236E-4	3.123525439804206E-4	7.951687791552032E-4	8.828639728910311E-4
52-53	1.7539038747165574E-4	2.650562597184011E-4	1.4385953129697607E-4	3.202352580240905E-4	8.385237063953878E-4	9.58735095561354E-4
54-55	1.8524378002624312E-4	2.72938973762071E-4	1.4878622757426974E-4	3.340300076005129E-4	9.006000794892884E-4	0.0010365768967425947
56-57	1.9017047630353684E-4	2.827923663166584E-4	1.546982631070222E-4	3.4388340015510026E-4	9.469110244958492E-4	0.001148905571864891
58-59	1.9608251183628926E-4	2.9363109812670455E-4	1.5863962012885714E-4	3.547221319651464E-4	9.971633265242448E-4	0.0012464541581553063
60-61	2.0100920811358297E-4	2.9954313365945697E-4	1.6849301268344455E-4	3.6851688154156874E-4	0.001068107752917274	0.0013558268155112263
62-63	2.088919221572529E-4	3.0742584770312694E-4	1.773610659825732E-4	3.8329697037344987E-4	0.001148905571864891	0.0014415513307361367
64-65	2.1086260066817037E-4	3.2319127579046676E-4	1.8524378002624312E-4	3.98077059205331E-4	0.0012287180515570487	0.0015519093273475156
66-67	2.187453147118403E-4	3.320593290895954E-4	1.9115581555899556E-4	4.1088646952629464E-4	0.001315427906037418	0.0016583259669370595
68-69	2.2367201098913398E-4	3.4289806089964157E-4	1.921411548144543E-4	4.2665189761363447E-4	0.001391299028707741	0.0017834640523803195
70-71	2.285987072664277E-4	3.517661141987702E-4	1.990385296026655E-4	4.355199509127631E-4	0.0014799795616990275	0.0019204262088890842
72-73	2.3056938577734518E-4	3.5964882824244014E-4	2.0495056513541792E-4	4.404466471900568E-4	0.0015607773806466442	0.002065271079441519
74-75	2.3155472503280392E-4	3.6851688154156874E-4	2.1283327917908783E-4	4.463586827228093E-4	0.0016524139314043071	0.002221940021059459
76-77	2.3943743907647383E-4	3.823116311179912E-4	2.1480395769000533E-4	4.591680930437729E-4	0.001758830570993851	0.002364814213100976
78-79	2.532321886528962E-4	4.000477377162485E-4	2.1973065396729903E-4	4.808455566638651E-4	0.0018790419601598172	0.0024840402630114835
80-81	2.5914422418564867E-4	4.158131658035883E-4	2.2268667173367526E-4	4.916842884739113E-4	0.001985458599749361	0.002647606579417635
82-83	2.630855812074836E-4	4.227105405917995E-4	2.2761336801096897E-4	5.025230202839574E-4	0.002096801935616199	0.002850586466042135
84-85	2.6801227748477727E-4	4.365052901682218E-4	2.414081175873913E-4	5.15332430604921E-4	0.0022120866285048717	0.00308115585181948
86-87	2.7392431301752974E-4	4.4340266495643304E-4	2.5027617088652E-4	5.291271801813435E-4	0.002335254035437214	0.003336358718983294
88-89	2.7589499152844724E-4	4.4931470048918546E-4	2.6012956344110736E-4	5.488339652905182E-4	0.0024456120320485927	0.003566928104760639
>>END_MODULE
