##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674219
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38194556
Total Bases	3.8 Gbp
Sequences flagged as poor quality	0
Sequence length	62-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.849731123985315	37.0	35.0	37.0	29.0	37.0
2	34.33394397882253	37.0	34.0	37.0	28.0	37.0
3	34.61694771370035	37.0	35.0	37.0	29.0	37.0
4	34.629179247429924	37.0	35.0	37.0	29.0	37.0
5	34.62663158068914	37.0	35.0	37.0	29.0	37.0
6	34.457941911931115	37.0	34.0	37.0	28.0	37.0
7	34.56465641333807	37.0	34.0	37.0	28.0	37.0
8	34.5226466044009	37.0	34.0	37.0	28.0	37.0
9	34.583246523405066	37.0	34.0	37.0	29.0	37.0
10-11	34.578110922404754	37.0	34.0	37.0	28.0	37.0
12-13	34.55371444820565	37.0	34.0	37.0	28.0	37.0
14-15	34.57400375330977	37.0	34.0	37.0	28.0	37.0
16-17	34.52148942378071	37.0	34.0	37.0	28.0	37.0
18-19	34.48611559982527	37.0	34.0	37.0	28.0	37.0
20-21	34.441935835044134	37.0	34.0	37.0	28.0	37.0
22-23	34.52391689014529	37.0	34.0	37.0	28.0	37.0
24-25	34.49917778858327	37.0	34.0	37.0	28.0	37.0
26-27	34.46914391412221	37.0	34.0	37.0	28.0	37.0
28-29	34.52574228117746	37.0	34.0	37.0	28.0	37.0
30-31	34.54278057323143	37.0	34.0	37.0	28.0	37.0
32-33	34.45893726320578	37.0	34.0	37.0	28.0	37.0
34-35	34.44432360988827	37.0	34.0	37.0	28.0	37.0
36-37	34.46430438934806	37.0	34.0	37.0	28.0	37.0
38-39	34.46349621134489	37.0	34.0	37.0	28.0	37.0
40-41	34.46828051882577	37.0	34.0	37.0	28.0	37.0
42-43	34.50871768217439	37.0	34.0	37.0	28.0	37.0
44-45	34.507874630091266	37.0	34.0	37.0	28.0	37.0
46-47	34.43265765414317	37.0	34.0	37.0	28.0	37.0
48-49	34.514040980081035	37.0	34.0	37.0	28.0	37.0
50-51	34.478174507382676	37.0	34.0	37.0	28.0	37.0
52-53	34.39320254174443	37.0	34.0	37.0	28.0	37.0
54-55	34.44765826051231	37.0	34.0	37.0	28.0	37.0
56-57	34.44163354065433	37.0	34.0	37.0	28.0	37.0
58-59	34.2380503389017	37.0	34.0	37.0	27.5	37.0
60-61	34.39727897347465	37.0	34.0	37.0	28.0	37.0
62-63	34.42323403168465	37.0	34.0	37.0	28.0	37.0
64-65	34.43161932418637	37.0	34.0	37.0	28.0	37.0
66-67	34.393225472620756	37.0	34.0	37.0	28.0	37.0
68-69	34.406891104662876	37.0	34.0	37.0	28.0	37.0
70-71	34.36536219905548	37.0	34.0	37.0	28.0	37.0
72-73	34.316529745098045	37.0	34.0	37.0	27.5	37.0
74-75	34.29903629829832	37.0	34.0	37.0	27.5	37.0
76-77	34.335534486339924	37.0	34.0	37.0	28.0	37.0
78-79	34.291666194323795	37.0	34.0	37.0	27.0	37.0
80-81	34.24767197559455	37.0	34.0	37.0	27.0	37.0
82-83	34.21177889542456	37.0	34.0	37.0	27.0	37.0
84-85	34.14452767873303	37.0	34.0	37.0	27.0	37.0
86-87	34.09662576102755	37.0	34.0	37.0	27.0	37.0
88-89	34.03523445805688	37.0	34.0	37.0	26.5	37.0
90-91	34.02177468914922	37.0	34.0	37.0	26.5	37.0
92-93	34.00883469462208	37.0	34.0	37.0	26.0	37.0
94-95	33.86257262752474	37.0	33.5	37.0	26.0	37.0
96-97	33.993055096439114	37.0	34.0	37.0	26.0	37.0
98-99	34.02709012253147	37.0	34.0	37.0	26.5	37.0
100	34.73335012599603	37.0	34.0	37.0	29.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	185.0
20	10977.0
21	59595.0
22	114185.0
23	173270.0
24	235717.0
25	308933.0
26	404495.0
27	531761.0
28	697425.0
29	910497.0
30	1188752.0
31	1560935.0
32	2105464.0
33	2962555.0
34	4536027.0
35	8316447.0
36	1.3524869E7
37	552467.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	24.225396309184816	29.830854638361753	25.11380707052214	20.829941981931295
2	26.475404978328953	34.02359801555702	23.852857657758825	15.6481393483552
3	15.569650647321623	29.806848823817887	31.411563841730338	23.211936687130148
4	18.542633518556745	30.46895874963195	30.312578915547135	20.675828816264175
5	20.892114497576667	28.23297734260605	29.896811719391174	20.97809644042611
6	20.586606850201008	28.660536556131134	29.669391199097845	21.083465394570016
7	21.08316591868166	29.46924937581891	29.193790407886517	20.253794297612906
8	19.577300869706757	28.00442960405196	30.7476173416125	21.670652184628782
9	20.277048650144494	27.40935941242462	30.752873783924517	21.560718153506368
10-11	21.309051345418094	28.208249536268415	29.069945753953057	21.412753364360434
12-13	21.27453917838959	28.546415991418066	28.690634433838458	21.488410396353892
14-15	21.409660586016845	28.57710620973526	28.49548410441278	21.51774909983512
16-17	21.490794790610863	28.562577672095234	28.57865574260629	21.36797179468761
18-19	21.382819896013956	28.48423467676749	28.58898809926821	21.54395732795035
20-21	21.506142354213573	28.320484532937307	28.560953690042286	21.612419422806834
22-23	21.58825624800647	28.57737062109336	28.405287695198467	21.429085435701708
24-25	21.467272801962896	28.54798355258642	28.454367188690416	21.53037645676027
26-27	21.566475507558668	28.40514055802234	28.459436038681684	21.568947895737306
28-29	21.660957790341605	28.47721791204407	28.39838164114981	21.463442656464508
30-31	21.542374314930424	28.443961962867952	28.412631293315716	21.601032428885905
32-33	21.623385040379567	28.342406862505857	28.37259510806106	21.661612989053513
34-35	21.663809377654143	28.49330381773912	28.336386663883005	21.50650014072373
36-37	21.5752228486335	28.43653587917949	28.388272864716146	21.599968407470858
38-39	21.65813575459743	28.322319319815364	28.355871321979677	21.663673603607528
40-41	21.722348083460574	28.412413971939028	28.314317368802822	21.550920575797576
42-43	21.587240639482538	28.395787904166653	28.359246591233518	21.65772486511729
44-45	21.642181743261396	28.334515367730273	28.335293090849216	21.688009798159115
46-47	21.68354479216641	28.42710806051712	28.355628648819124	21.533718498497347
48-49	21.588832694306966	28.393060485863387	28.36060265476363	21.65750416506602
50-51	21.676356086187024	28.262499707523908	28.344005227965884	21.717138978323177
52-53	21.71217487194793	28.37946869726838	28.320748333646844	21.587608097136844
54-55	21.581618701285255	28.37322450723667	28.354809900827465	21.690346890650606
56-57	21.657804721373292	28.288630434347844	28.363825660809127	21.68973918346974
58-59	21.7084227889385	28.365323450985947	28.35415749188038	21.572096268195182
60-61	21.6226657885569	28.34369163538658	28.35882091090256	21.674821665153956
62-63	21.69118284444928	28.24546151592719	28.338796639867446	21.72455899975608
64-65	21.72362630863564	28.377257431223228	28.305291056054934	21.593825204086198
66-67	21.60221732928387	28.35947932062359	28.37076846717314	21.667534882919398
68-69	21.655170781480585	28.259253993282506	28.38135507762722	21.70422014760969
70-71	21.72885953652081	28.356896478314603	28.34452647528914	21.569717509875446
72-73	21.62519555042935	28.324387469902174	28.35556456195038	21.694852417718096
74-75	21.69165622576062	28.228346941911504	28.347247403059534	21.732749429268345
76-77	21.722582828552333	28.357650710649924	28.33169370582434	21.588072754973403
78-79	21.604021219201467	28.33463958731699	28.385887857453767	21.675451336027773
80-81	21.678155200157438	28.2391283805544	28.373574412687486	21.709142006600676
82-83	21.754435392816298	28.341631180581718	28.30686040094485	21.59707302565714
84-85	21.65326445075517	28.31229072124268	28.331281611972077	21.703163216030077
86-87	21.69956184205012	28.25354336631365	28.313973656881767	21.732921134754463
88-89	21.749062645511653	28.354505486280683	28.3187860116772	21.577645856530463
90-91	21.638701118818787	28.32635588799159	28.363053069858342	21.671889923331282
92-93	21.714188394960562	28.22893042458971	28.337659504853306	21.71922167559642
94-95	21.771979165806094	28.358804327400396	28.27861269205203	21.59060381474148
96-97	21.637783977392026	28.44669922875148	28.268458207763974	21.64705858609252
98-99	21.68572716166254	28.29508482759355	28.324785471289438	21.694402539454476
100	22.00268290663989	27.91121053486204	28.17268918511948	21.91341737337859
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	5.5
2	5.0
3	4.0
4	7.5
5	10.5
6	12.0
7	32.5
8	64.0
9	156.5
10	273.0
11	479.0
12	833.0
13	1434.0
14	2443.0
15	4167.0
16	6702.5
17	10491.0
18	16267.5
19	24564.5
20	37089.5
21	55828.5
22	80968.5
23	113776.5
24	153020.0
25	200047.5
26	256760.0
27	317767.0
28	381396.5
29	453270.0
30	535744.5
31	624515.5
32	720220.0
33	826339.5
34	943325.0
35	1065363.0
36	1195372.0
37	1329946.5
38	1457759.5
39	1574571.0
40	1676511.5
41	1757821.5
42	1811669.5
43	1837208.5
44	1827178.5
45	1781176.0
46	1707504.0
47	1603720.5
48	1477686.0
49	1344655.5
50	1212459.0
51	1082460.0
52	951966.0
53	833511.5
54	731808.5
55	639315.5
56	555852.5
57	482800.0
58	419981.5
59	366023.0
60	316970.0
61	271327.0
62	229720.5
63	191754.5
64	157924.0
65	128632.5
66	103315.5
67	81605.0
68	63727.0
69	48936.0
70	36728.5
71	26903.5
72	19265.5
73	13342.5
74	9018.0
75	5979.0
76	3949.0
77	2521.0
78	1640.0
79	1071.5
80	647.0
81	406.5
82	273.0
83	197.5
84	120.5
85	73.5
86	49.0
87	36.0
88	29.5
89	18.0
90	9.5
91	5.5
92	5.5
93	7.0
94	7.0
95	5.0
96	2.5
97	1.5
98	2.5
99	3.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02727352034148531
2	0.0254879255567207
3	4.5294413161917627E-4
4	0.054552277031313046
5	0.0012829053438924646
6	0.18544004019839894
7	0.07159920906005557
8	0.14348641727894415
9	0.05320653550731156
10-11	0.035410805665603236
12-13	0.0510020328551535
14-15	0.002247702525983022
16-17	0.03985777449540191
18-19	0.13522738685586502
20-21	0.24402430545337403
22-23	0.055243475012512255
24-25	0.16040374968621182
26-27	0.1924868559802083
28-29	0.07360603956228737
30-31	9.202882211800026E-4
32-33	0.2191307054335178
34-35	0.16987106748930397
36-37	0.12121230051738263
38-39	0.07833184394131981
40-41	0.14429019674950533
42-43	0.045083650141135294
44-45	0.016122716546305708
46-47	0.2096751694141961
48-49	0.0010040697946586943
50-51	0.07603963245442623
52-53	0.21170949074522555
54-55	0.07645199488639166
56-57	0.04694517197686498
58-59	0.48744512176028437
60-61	0.00103156062345639
62-63	0.0264671233897083
64-65	0.02813752113308622
66-67	0.031041079881886285
68-69	9.687247603836396E-4
70-71	0.014083165872648228
72-73	0.20525717246796948
74-75	0.15514138410788325
76-77	0.011581510426893924
78-79	0.04346178455029909
80-81	0.07375031719299671
82-83	0.04470045276979431
84-85	0.1224380776766236
86-87	0.13414183329327856
88-89	0.0899070123018494
90-91	0.030113995520657723
92-93	0.05976100129607424
94-95	0.1380986806845942
96-97	0.0022108123453700905
98-99	0.042342430320285056
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62	2.0
63	1.0
64	3.0
65	1.0
66	3.0
67	2.0
68	1.0
69	4.0
70	4.0
71	10.0
72	2.0
73	6.0
74	3.0
75	8.0
76	12.0
77	14.0
78	12.0
79	27.0
80	58.0
81	87.0
82	104.0
83	115.0
84	153.0
85	226.0
86	338.0
87	328.0
88	476.0
89	610.0
90	803.0
91	1282.0
92	2153.0
93	3189.0
94	6252.0
95	11230.0
96	27281.0
97	70524.0
98	259494.0
99	1579629.0
100	3.6230109E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.08113902634365
#Duplication Level	Percentage of total
1	55.0272320380415
2	18.88928597317116
3	10.968906474382123
4	6.334013187256196
5	3.5053363726731415
6	1.8629869918092634
7	1.092694223704635
8	0.5803096401315884
9	0.385362006566051
>10	1.2349042263587304
>50	0.08783583589256468
>100	0.023905586706283687
>500	0.0
>1k	0.0072274433067879605
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.6181741712091115E-6	5.236348342418223E-6	0.0	0.0	2.3563567540882005E-5	1.570904502725467E-5
2	2.6181741712091115E-6	1.0472696684836446E-5	0.0	0.0	4.189078673934578E-5	3.141809005450934E-5
3	2.6181741712091115E-6	1.570904502725467E-5	0.0	7.854522513627334E-6	5.498165759539134E-5	3.6654438396927564E-5
4	7.854522513627334E-6	2.094539336967289E-5	0.0	7.854522513627334E-6	7.592705096506423E-5	4.189078673934578E-5
5	7.854522513627334E-6	2.6181741712091115E-5	0.0	1.0472696684836446E-5	8.116339930748246E-5	4.712713508176401E-5
6	7.854522513627334E-6	2.8799915883300228E-5	0.0	1.0472696684836446E-5	9.16360959923189E-5	6.283618010901867E-5
7	7.854522513627334E-6	3.141809005450934E-5	0.0	1.0472696684836446E-5	1.0472696684836446E-4	7.069070262264601E-5
8	7.854522513627334E-6	4.189078673934578E-5	2.6181741712091115E-6	1.0472696684836446E-5	1.125814893619918E-4	8.378157347869157E-5
9	7.854522513627334E-6	4.4508960910554896E-5	2.6181741712091115E-6	1.570904502725467E-5	1.335268827316647E-4	1.1781783770441002E-4
10-11	7.854522513627334E-6	4.4508960910554896E-5	5.236348342418223E-6	1.570904502725467E-5	1.6101771152936037E-4	1.3745414398847836E-4
12-13	9.16360959923189E-6	4.843622216736856E-5	7.854522513627334E-6	2.2254480455277448E-5	1.7672675655661503E-4	1.6887223404298771E-4
14-15	1.0472696684836446E-5	5.498165759539134E-5	7.854522513627334E-6	2.6181741712091115E-5	2.0290849826870615E-4	1.9112671449826514E-4
16-17	1.3090870856045557E-5	6.80725284514369E-5	9.16360959923189E-6	2.6181741712091115E-5	2.2647206580958816E-4	2.1469028203914716E-4
18-19	1.3090870856045557E-5	7.461796387945968E-5	1.0472696684836446E-5	3.0109002968904784E-5	2.4479928500805196E-4	2.3432658832321548E-4
20-21	1.570904502725467E-5	7.854522513627335E-5	1.0472696684836446E-5	3.5345351311323004E-5	2.749082879769567E-4	2.487265462648656E-4
22-23	1.570904502725467E-5	8.247248639308701E-5	1.0472696684836446E-5	4.3199873824950337E-5	2.984718555178387E-4	2.670537654633294E-4
24-25	1.570904502725467E-5	8.509066056429612E-5	1.0472696684836446E-5	4.712713508176401E-5	3.2203542305872073E-4	2.906173330042114E-4
26-27	1.570904502725467E-5	9.163609599231891E-5	1.1781783770441002E-5	5.498165759539134E-5	3.455989905996027E-4	3.1679907471630247E-4
28-29	1.570904502725467E-5	9.556335724913257E-5	1.570904502725467E-5	6.152709302341412E-5	3.691625581404847E-4	3.4428990351399817E-4
30-31	1.570904502725467E-5	9.687244433473713E-5	1.7018132112859225E-5	7.199978970825056E-5	3.966533869381804E-4	3.639262097980665E-4
32-33	1.570904502725467E-5	1.0210879267715535E-4	1.8327219198463782E-5	7.330887679385513E-5	4.254533028214806E-4	3.757079935685075E-4
34-35	1.570904502725467E-5	1.0603605393396903E-4	1.8327219198463782E-5	7.854522513627335E-5	4.5687139287598996E-4	4.0974425779422593E-4
36-37	1.570904502725467E-5	1.1127240227638724E-4	2.094539336967289E-5	8.509066056429612E-5	4.7781678624566286E-4	4.4247143493433984E-4
38-39	1.570904502725467E-5	1.1389057644759636E-4	2.2254480455277448E-5	9.032700890671435E-5	4.974530925297312E-4	4.6865317664643096E-4
40-41	1.570904502725467E-5	1.2174509896122369E-4	2.3563567540882005E-5	9.425427016352802E-5	5.236348342418224E-4	4.895985700161039E-4
42-43	1.570904502725467E-5	1.269814473036419E-4	2.3563567540882005E-5	9.949061850594623E-5	5.445802276114952E-4	5.197075729850087E-4
44-45	1.570904502725467E-5	1.2829053438924646E-4	2.3563567540882005E-5	1.1781783770441003E-4	5.838528401796319E-4	5.445802276114952E-4
46-47	1.7018132112859225E-5	1.2829053438924646E-4	2.6181741712091115E-5	1.2174509896122369E-4	6.034891464637001E-4	5.864710143508409E-4
48-49	2.094539336967289E-5	1.309087085604556E-4	2.6181741712091115E-5	1.243632731324328E-4	6.231254527477685E-4	6.21816365662164E-4
50-51	2.3563567540882005E-5	1.4661775358771026E-4	2.6181741712091115E-5	1.2829053438924646E-4	6.532344557166733E-4	6.558526298878825E-4
52-53	2.6181741712091118E-5	1.5185410193012847E-4	2.6181741712091115E-5	1.3221779564606014E-4	6.833434586855781E-4	7.016706778840419E-4
54-55	3.403626422571845E-5	1.6232679861496492E-4	2.6181741712091115E-5	1.387632310740829E-4	7.186888099969011E-4	7.383251162809694E-4
56-57	3.6654438396927564E-5	1.6494497278617403E-4	2.6181741712091115E-5	1.4923592775891936E-4	7.396342033665741E-4	7.684341192498742E-4
58-59	3.6654438396927564E-5	1.6756314695738313E-4	2.6181741712091115E-5	1.5578136318694213E-4	7.60579596736247E-4	8.1032490598922E-4
60-61	3.6654438396927564E-5	1.7803584364221959E-4	2.6181741712091115E-5	1.5839953735815126E-4	7.815249901059198E-4	8.365066477013112E-4
62-63	3.7963525482532124E-5	1.9374488866947425E-4	2.6181741712091115E-5	1.6625405987177858E-4	8.063976447324063E-4	8.653065635846114E-4
64-65	3.927261256813668E-5	2.0552667243991526E-4	2.6181741712091115E-5	1.7279949529980137E-4	8.247248639308702E-4	8.941064794679116E-4
66-67	4.189078673934578E-5	2.0814484661112436E-4	2.6181741712091115E-5	1.7410858238540593E-4	8.626883894134023E-4	9.242154824368163E-4
68-69	4.3199873824950337E-5	2.159993691247517E-4	2.749082879769567E-5	1.7934493072782414E-4	8.941064794679115E-4	9.582517466625348E-4
70-71	4.4508960910554896E-5	2.1861754329596082E-4	2.8799915883300228E-5	1.8589036615584693E-4	9.307609178648391E-4	9.93597097973858E-4
72-73	4.4508960910554896E-5	2.2647206580958816E-4	2.8799915883300228E-5	1.9636306284068336E-4	9.661062691761623E-4	0.0010394151459700175
74-75	4.4508960910554896E-5	2.3301750123761093E-4	2.8799915883300228E-5	2.068357595255198E-4	9.962152721450668E-4	0.0010852331939661768
76-77	4.712713508176401E-5	2.3432658832321548E-4	2.8799915883300228E-5	2.1730845621035626E-4	0.001040724233055622	0.0011179603711062905
78-79	4.843622216736856E-5	2.3825384958002914E-4	2.8799915883300228E-5	2.2909023998079727E-4	0.0010943968035654087	0.0011611602449312411
80-81	5.1054396338577676E-5	2.4349019792244738E-4	2.8799915883300228E-5	2.3563567540882003E-4	0.001140214851561568	0.0012056692058417958
82-83	5.236348342418223E-5	2.461083720936565E-4	2.8799915883300228E-5	2.4349019792244738E-4	0.0011703238545304729	0.001260650863437187
84-85	5.236348342418223E-5	2.513447204360747E-4	2.8799915883300228E-5	2.461083720936565E-4	0.0012030510316705868	0.001314323433946974
86-87	5.236348342418223E-5	2.591992429497021E-4	2.8799915883300228E-5	2.513447204360747E-4	0.0012397054700675142	0.0013810868753128063
88-89	5.236348342418223E-5	2.6836285254893397E-4	2.8799915883300228E-5	2.539628946072838E-4	0.0012855235180636738	0.0014465412295930342
>>END_MODULE
