##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674221
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49790178
Total Bases	4.9 Gbp
Sequences flagged as poor quality	0
Sequence length	62-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.12818365501726	37.0	35.0	37.0	30.0	37.0
2	34.665167294641925	37.0	35.0	37.0	29.0	37.0
3	34.94643841200969	37.0	35.0	37.0	30.0	37.0
4	34.923139519605655	37.0	35.0	37.0	30.0	37.0
5	34.91340316156331	37.0	35.0	37.0	29.0	37.0
6	34.75790174921648	37.0	35.0	37.0	29.0	37.0
7	34.85473659081918	37.0	35.0	37.0	29.0	37.0
8	34.818160903140374	37.0	35.0	37.0	29.0	37.0
9	34.865362501817124	37.0	35.0	37.0	29.0	37.0
10-11	34.863842252180746	37.0	35.0	37.0	29.0	37.0
12-13	34.83718564934634	37.0	35.0	37.0	29.0	37.0
14-15	34.85167525410333	37.0	35.0	37.0	29.0	37.0
16-17	34.80086342531252	37.0	35.0	37.0	29.0	37.0
18-19	34.760416552437306	37.0	35.0	37.0	29.0	37.0
20-21	34.713346465642275	37.0	35.0	37.0	29.0	37.0
22-23	34.79538052665728	37.0	35.0	37.0	29.0	37.0
24-25	34.76599599623846	37.0	35.0	37.0	29.0	37.0
26-27	34.732281204136285	37.0	35.0	37.0	29.0	37.0
28-29	34.78551706523323	37.0	35.0	37.0	29.0	37.0
30-31	34.80203177823546	37.0	35.0	37.0	29.0	37.0
32-33	34.71378368038772	37.0	35.0	37.0	29.0	37.0
34-35	34.70212214344765	37.0	35.0	37.0	29.0	37.0
36-37	34.71777471853987	37.0	35.0	37.0	29.0	37.0
38-39	34.71528826428377	37.0	35.0	37.0	29.0	37.0
40-41	34.71366952132607	37.0	35.0	37.0	29.0	37.0
42-43	34.75321344502926	37.0	35.0	37.0	29.0	37.0
44-45	34.74600735510526	37.0	35.0	37.0	29.0	37.0
46-47	34.669575051529236	37.0	35.0	37.0	29.0	37.0
48-49	34.74952248614174	37.0	35.0	37.0	29.0	37.0
50-51	34.70783261710774	37.0	35.0	37.0	29.0	37.0
52-53	34.622425832661214	37.0	35.0	37.0	28.5	37.0
54-55	34.67253523375635	37.0	35.0	37.0	29.0	37.0
56-57	34.665123410484696	37.0	35.0	37.0	29.0	37.0
58-59	34.46119212508138	37.0	35.0	37.0	28.5	37.0
60-61	34.62101601645208	37.0	35.0	37.0	29.0	37.0
62-63	34.63836597087727	37.0	35.0	37.0	29.0	37.0
64-65	34.640254316512944	37.0	35.0	37.0	29.0	37.0
66-67	34.60138181883272	37.0	35.0	37.0	29.0	37.0
68-69	34.60972383348498	37.0	35.0	37.0	29.0	37.0
70-71	34.56862677298494	37.0	35.0	37.0	28.0	37.0
72-73	34.510646545923606	37.0	35.0	37.0	28.0	37.0
74-75	34.488727578465664	37.0	35.0	37.0	28.0	37.0
76-77	34.523874196637564	37.0	35.0	37.0	28.0	37.0
78-79	34.47755238184932	37.0	35.0	37.0	28.0	37.0
80-81	34.42782416467913	37.0	34.0	37.0	28.0	37.0
82-83	34.38989457034877	37.0	34.0	37.0	28.0	37.0
84-85	34.318492793726485	37.0	34.0	37.0	27.5	37.0
86-87	34.262004934207454	37.0	34.0	37.0	27.5	37.0
88-89	34.198179093739	37.0	34.0	37.0	27.0	37.0
90-91	34.18297937496506	37.0	34.0	37.0	27.0	37.0
92-93	34.15654987600139	37.0	34.0	37.0	27.0	37.0
94-95	34.010548074813975	37.0	34.0	37.0	26.0	37.0
96-97	34.12859104241829	37.0	34.0	37.0	27.0	37.0
98-99	34.15736391852044	37.0	34.0	37.0	27.0	37.0
100	34.85557284117824	37.0	34.0	37.0	29.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	296.0
20	12659.0
21	62551.0
22	119376.0
23	185640.0
24	259841.0
25	346036.0
26	460338.0
27	612722.0
28	807505.0
29	1059858.0
30	1387710.0
31	1830453.0
32	2475796.0
33	3511046.0
34	5449479.0
35	1.0494032E7
36	1.9734683E7
37	980157.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	26.907639622957603	26.730635437332044	22.635871510313773	23.725853429396583
2	26.210205376421786	30.875095045962077	24.983017838697464	17.931681738918673
3	17.730705046514508	27.99016725243081	29.96999454648969	24.30913315456499
4	20.18727842794041	28.882261599235026	28.81707206891474	22.11338790390983
5	22.05258612550219	27.18964297728432	28.52591010064146	22.231860796572033
6	21.716541225691536	28.18758315444814	28.042716334370827	22.053159285489492
7	22.062975639495626	28.1384847997054	28.244723628604508	21.55381593219447
8	20.895683415470494	27.142476079817925	29.12025847831788	22.84158202639371
9	21.288234412178177	26.423293832149742	29.552922304077022	22.735549451595062
10-11	22.495225887974186	26.978508213128233	27.875608501255613	22.650657397641968
12-13	22.49043814026617	27.3804572992015	27.50714698608949	22.62195757444284
14-15	22.495273562943208	27.487423449595084	27.412987463719464	22.60431552374225
16-17	22.670107478919373	27.436930413716233	27.38322963177281	22.509732475591584
18-19	22.53520247216881	27.397812167892017	27.44925350529398	22.617731854645193
20-21	22.573376988698897	27.315804576887263	27.428237260505846	22.682581173907995
22-23	22.70480595543089	27.468063293695195	27.308963538893476	22.51816721198044
24-25	22.568906224469224	27.469222251016877	27.40903040617048	22.552841118343416
26-27	22.616439296315498	27.367525834411623	27.417825407706914	22.59820946156596
28-29	22.765444595961785	27.36276628755374	27.297104418922764	22.57468469756171
30-31	22.6415472533896	27.366393642125896	27.346732958926097	22.645326145558407
32-33	22.647736568838766	27.335704408041543	27.328374476394995	22.688184546724695
34-35	22.753008728137257	27.405258344887784	27.26466144744309	22.577071479531867
36-37	22.640448179138776	27.377911717116778	27.36195030570652	22.619689798037925
38-39	22.679669671905128	27.29734634972525	27.33816164212548	22.684822336244142
40-41	22.802148360708618	27.317338376767868	27.240505180852775	22.640008081670736
42-43	22.662674756981612	27.357803538767655	27.30925461635722	22.67026708789351
44-45	22.65978118372559	27.327279433951084	27.318695028089294	22.694244354234026
46-47	22.76263426114541	27.36875728871233	27.279912034090493	22.588696416051764
48-49	22.65124459937642	27.342364023155657	27.353822207916284	22.65256916955164
50-51	22.69141153624005	27.27482185170034	27.317395178884755	22.71637143317485
52-53	22.78651768072623	27.314850889825117	27.25505934869487	22.64357208075378
54-55	22.655882322466304	27.335908408415122	27.332910520212796	22.675298748905778
56-57	22.660866016128647	27.293509008310433	27.349627969375927	22.695997006184992
58-59	22.784085780062444	27.297420921504933	27.294263206029832	22.62423009240279
60-61	22.685923048517427	27.30755250417667	27.337580828532705	22.6689436187732
62-63	22.689925406079876	27.25184370363369	27.32794535290455	22.73028553738189
64-65	22.794488500720337	27.305139115937273	27.25296321869456	22.647409164647833
66-67	22.663379965220763	27.320864987076522	27.342838075156678	22.672916972546037
68-69	22.670709703643237	27.269761339581635	27.355773218218744	22.703755738556385
70-71	22.80412947075911	27.291467755821046	27.27127716358045	22.633125609839396
72-73	22.66346634514115	27.29264846487573	27.347604073914212	22.69628111606891
74-75	22.698754337486278	27.236536274220157	27.334835967328058	22.729873420965504
76-77	22.802291001198313	27.273591548864335	27.272342155809238	22.651775294128115
78-79	22.66116679111767	27.28911543288714	27.360990054756968	22.688727721238227
80-81	22.693632943896482	27.22816664096483	27.350996801666383	22.727203613472305
82-83	22.81747497507205	27.27347448309873	27.256104513506575	22.652946028322646
84-85	22.711070999232216	27.27320030453925	27.31566265641722	22.700066039811308
86-87	22.721267852954842	27.240781359639982	27.304455699386885	22.733495088018294
88-89	22.820622126234774	27.27252056848612	27.25090087661779	22.655956428661312
90-91	22.732478141204602	27.26605662744933	27.314241993429917	22.68722323791615
92-93	22.746924954969025	27.21293713064056	27.318485519311608	22.721652395078802
94-95	22.861269820482917	27.284538499527894	27.202962093116273	22.651229586872923
96-97	22.71877078804751	27.453046910985112	27.19995802478251	22.628224276184866
98-99	22.733137899314045	27.326755003544605	27.26239133384249	22.67771576329886
100	23.18032689530602	26.799657201601295	27.049031121295442	22.970984781797238
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	26.0
2	12.5
3	15.0
4	24.0
5	68.5
6	134.0
7	196.0
8	307.0
9	532.0
10	916.5
11	1638.0
12	2927.5
13	4856.5
14	8092.5
15	13007.5
16	19669.0
17	28779.0
18	40954.0
19	57213.0
20	78374.5
21	103813.0
22	135164.5
23	173212.0
24	217573.0
25	270441.0
26	330378.0
27	397268.5
28	471254.0
29	551566.5
30	636096.5
31	723044.5
32	817150.5
33	923451.5
34	1039361.5
35	1157619.0
36	1278134.0
37	1398695.0
38	1513979.0
39	1622274.5
40	1721973.0
41	1808243.5
42	1878107.5
43	1930159.0
44	1958755.0
45	1961336.0
46	1942208.0
47	1896886.0
48	1825102.0
49	1740909.5
50	1645283.0
51	1538663.5
52	1429689.0
53	1321257.5
54	1213635.5
55	1109460.5
56	1013622.0
57	929225.5
58	855995.0
59	790104.5
60	727252.0
61	667065.0
62	608174.0
63	548253.5
64	489616.0
65	432223.0
66	374254.0
67	317825.0
68	264129.0
69	214529.5
70	170175.5
71	131499.0
72	99418.5
73	73271.0
74	52505.0
75	36838.5
76	25331.0
77	16999.0
78	11052.5
79	7100.0
80	4438.5
81	2692.5
82	1648.5
83	1014.5
84	645.0
85	430.5
86	290.5
87	200.0
88	124.0
89	80.0
90	65.5
91	43.0
92	24.0
93	14.5
94	9.0
95	7.0
96	6.5
97	7.0
98	6.0
99	3.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02689084582103723
2	0.025999103678641198
3	4.5993006893849624E-4
4	0.055404501666975366
5	0.001319537359356297
6	0.1877840243913167
7	0.07278744815895215
8	0.14484784529189673
9	0.05394437433021428
10-11	0.03634250915913576
12-13	0.05155534892845733
14-15	0.002185169934520017
16-17	0.040074168845108366
18-19	0.13756227181995614
20-21	0.24728873232789006
22-23	0.05624000781840949
24-25	0.16058488483411326
26-27	0.19464381107454568
28-29	0.07390313005107152
30-31	9.108222107581138E-4
32-33	0.2215597622486909
34-35	0.1706280302914362
36-37	0.12224599799582961
38-39	0.07876553484102829
40-41	0.14608403287893448
42-43	0.04589861076616356
44-45	0.016165838973301122
46-47	0.21184097795352327
48-49	8.867210717744372E-4
50-51	0.07706339190030612
52-53	0.21267748831908176
54-55	0.07730339907601858
56-57	0.047350704389930075
58-59	0.49179478731728976
60-61	0.0010443826892926552
62-63	0.02677736989389122
64-65	0.028753665620385917
66-67	0.03142387342722469
68-69	0.0010694882786796036
70-71	0.014199593849438896
72-73	0.20824098951540607
74-75	0.15641450295178924
76-77	0.011945140803585724
78-79	0.044503851646329695
80-81	0.07373072359985486
82-83	0.045609894051142436
84-85	0.12377481854425618
86-87	0.13558157786515854
88-89	0.0915916482741715
90-91	0.030295038422926572
92-93	0.06038470119782492
94-95	0.13805405523892106
96-97	0.0020136680306293486
98-99	0.04183910030224216
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62	1.0
63	2.0
64	3.0
65	1.0
66	2.0
67	1.0
68	2.0
69	8.0
70	3.0
71	3.0
72	5.0
73	4.0
74	6.0
75	7.0
76	19.0
77	22.0
78	33.0
79	38.0
80	110.0
81	172.0
82	201.0
83	190.0
84	277.0
85	343.0
86	500.0
87	559.0
88	751.0
89	933.0
90	1204.0
91	1736.0
92	2786.0
93	4445.0
94	8005.0
95	15137.0
96	35119.0
97	91241.0
98	335577.0
99	2040803.0
100	4.7249929E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.55829611609119
#Duplication Level	Percentage of total
1	75.96788814769285
2	14.743945868315578
3	4.146276259139301
4	1.6527618276979767
5	0.7685194349702809
6	0.47804563629554686
7	0.35587857360700664
8	0.24050965047542222
9	0.19239946224758298
>10	1.3393507143594072
>50	0.08777473704005055
>100	0.02243916276467037
>500	0.004210525394391883
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.205056949183833E-5	4.0168564972794435E-6	6.025284745919165E-6	1.0042141243198608E-5	3.0126423729595827E-5
2	8.033712994558887E-6	1.8075854237757495E-5	1.0042141243198608E-5	1.4058997740478051E-5	3.0126423729595827E-5	4.820227796735332E-5
3	1.0042141243198608E-5	3.0126423729595827E-5	1.205056949183833E-5	2.0084282486397216E-5	3.414328022687527E-5	5.6235990961912205E-5
4	1.6067425989117774E-5	3.615170847551499E-5	1.205056949183833E-5	2.610956723231638E-5	5.221913446463276E-5	6.226127570783137E-5
5	1.8075854237757495E-5	5.021070621599304E-5	1.4058997740478051E-5	2.8117995480956103E-5	6.0252847459191654E-5	7.029498870239026E-5
6	2.209271073503694E-5	5.8244419210551926E-5	1.8075854237757495E-5	3.213485197823555E-5	6.828656045375054E-5	8.636241469150803E-5
7	2.8117995480956103E-5	6.226127570783137E-5	2.610956723231638E-5	3.816013672415471E-5	7.230341695102998E-5	9.640455593470664E-5
8	3.615170847551499E-5	6.828656045375054E-5	2.610956723231638E-5	4.217699322143415E-5	8.636241469150803E-5	1.0644669717790525E-4
9	4.016856497279443E-5	7.632027344830942E-5	3.0126423729595827E-5	4.61938497187136E-5	8.837084294014776E-5	1.385815491561408E-4
10-11	4.5189635594393744E-5	9.439612768606692E-5	3.3139066102555405E-5	5.8244419210551926E-5	1.2954362203726206E-4	1.58665831642538E-4
12-13	5.021070621599304E-5	1.0644669717790525E-4	3.514749435119513E-5	7.431184519966969E-5	1.5766161751821817E-4	1.9783018249101258E-4
14-15	6.226127570783138E-5	1.1749305254542372E-4	4.016856497279443E-5	8.033712994558886E-5	1.8678382712349412E-4	2.3197346271788786E-4
16-17	6.828656045375054E-5	1.3456469265886136E-4	4.61938497187136E-5	9.23876994374272E-5	2.0184703898829204E-4	2.520577452042851E-4
18-19	7.129920282671012E-5	1.5264054689661884E-4	5.623599096191221E-5	1.255267655399826E-4	2.2996503446924815E-4	2.771630983122816E-4
20-21	7.732448757262928E-5	1.616784740154976E-4	7.029498870239026E-5	1.4058997740478053E-4	2.5004931695564534E-4	3.042768796689178E-4
22-23	8.937505706446762E-5	1.7372904350733593E-4	7.632027344830942E-5	1.566574033938983E-4	2.7314624181500215E-4	3.3440330339851366E-4
24-25	9.841298418334636E-5	1.9180489774509342E-4	8.134134406990873E-5	1.6971218701005648E-4	3.0126423729595825E-4	3.6252129887946976E-4
26-27	1.0945933955086484E-4	2.0887653785853105E-4	8.43539864428683E-5	1.7774590000461536E-4	3.2436116215531506E-4	3.8561822373882657E-4
28-29	1.1448041017246414E-4	2.179144649774098E-4	8.837084294014775E-5	1.94817540118053E-4	3.46453872890352E-4	4.077109344738635E-4
30-31	1.2251412316702302E-4	2.2996503446924815E-4	9.640455593470663E-5	2.1188918023149064E-4	3.6754236950106906E-4	4.25786788711621E-4
32-33	1.3958576328046066E-4	2.440240322097262E-4	1.024298406806258E-4	2.3197346271788786E-4	3.8662243786314644E-4	4.7198063843033463E-4
34-35	1.436026197777401E-4	2.5507038757724464E-4	1.0745091130222511E-4	2.4804088870700565E-4	4.046982921009039E-4	5.181744881490482E-4
36-37	1.51636332772299E-4	2.7013359944204254E-4	1.1448041017246414E-4	2.6611674294476316E-4	4.197615039657018E-4	5.583430531218426E-4
38-39	1.58665831642538E-4	2.872052395554802E-4	1.1849726666974358E-4	2.8117995480956106E-4	4.338205017061799E-4	5.874652627271186E-4
40-41	1.6870797288573664E-4	3.042768796689178E-4	1.2753519378862232E-4	2.982515949229987E-4	4.5892585481417637E-4	6.27633827699913E-4
42-43	1.8276697062621466E-4	3.2134851978235545E-4	1.3456469265886136E-4	3.183358774093959E-4	4.8202277967353323E-4	6.678023926727074E-4
44-45	1.897964694964537E-4	3.384201598957931E-4	1.5464897514525856E-4	3.3540751752283353E-4	4.990944197869709E-4	7.170088847643806E-4
46-47	1.9280911186941329E-4	3.585044423821903E-4	1.5967004576685785E-4	3.504707293876314E-4	5.211871305220078E-4	7.601900921101346E-4
48-49	1.998386107396523E-4	3.705550118740286E-4	1.6569533051277702E-4	3.6051287063083E-4	5.472966977543242E-4	8.043755135802085E-4
50-51	2.0887653785853105E-4	3.8360979549018683E-4	1.7272482938301606E-4	3.705550118740286E-4	5.764189073596002E-4	8.415314361800434E-4
52-53	2.1188918023149064E-4	3.9766879323066486E-4	1.7573747175597565E-4	3.89635080236106E-4	6.005200463432768E-4	9.01784283639235E-4
54-55	2.2695239209628853E-4	4.1072357684682307E-4	1.7875011412893523E-4	4.0570250622522377E-4	6.316506841971925E-4	9.630413452227465E-4
56-57	2.30969248593568E-4	4.177530757170621E-4	1.8276697062621466E-4	4.2679100283594083E-4	6.647897502997479E-4	0.0010242984068062583
58-59	2.4502824633404604E-4	4.237783604629813E-4	1.897964694964537E-4	4.4285842882505865E-4	6.908993175320643E-4	0.0010765175412708909
60-61	2.540661734529248E-4	4.3181207345754013E-4	1.9883439661533246E-4	4.5892585481417637E-4	7.230341695102998E-4	0.001146812529973281
62-63	2.67120957069083E-4	4.458710711980182E-4	2.0586389548557147E-4	4.699722101816949E-4	7.521563791155758E-4	0.0012150990904270316
64-65	2.711378135663624E-4	4.649511395600956E-4	2.098807519828509E-4	4.800143514248935E-4	7.862996593424509E-4	0.001290415149751021
66-67	2.781673124366015E-4	4.749932808032942E-4	2.1590603672877007E-4	5.000986339112907E-4	8.164260830720468E-4	0.0013667354231993306
68-69	2.882094536798001E-4	4.850354220464928E-4	2.2193132147468924E-4	5.171702740247283E-4	8.405272220557235E-4	0.0014370304119017208
70-71	3.0126423729595825E-4	4.980902056626509E-4	2.259481779719687E-4	5.312292717652064E-4	8.776831446555583E-4	0.0015123464712257103
72-73	3.123105926634767E-4	5.071281327815297E-4	2.3498610509084744E-4	5.633641237434419E-4	9.429570627363493E-4	0.0015836456740524205
74-75	3.2536537627963493E-4	5.201829163976879E-4	2.37998747463807E-4	5.975074039703172E-4	9.861382700821033E-4	0.0016830628723600869
76-77	3.273738045282746E-4	5.322334858895262E-4	2.4402403220972617E-4	6.175916864567144E-4	0.0010303236915521772	0.0017573747175597563
78-79	3.3540751752283353E-4	5.422756271327249E-4	2.5004931695564534E-4	6.256253994512734E-4	0.0010745091130222512	0.0018377118475053453
80-81	3.4042858814443283E-4	5.543261966245632E-4	2.6009145819884394E-4	6.366717548187918E-4	0.0011227113909896046	0.0019361248316886919
82-83	3.434412305173924E-4	5.673809802407214E-4	2.681251711934028E-4	6.467138960619904E-4	0.0011598673135894393	0.0020465883853638765
84-85	3.484623011389917E-4	5.864610486027988E-4	2.7515467006364184E-4	6.607728938024685E-4	0.0011779431678271969	0.00216608986615794
86-87	3.615170847551499E-4	5.944947615973575E-4	2.821841689338809E-4	6.768403197915862E-4	0.0012100780198054323	0.002287599775200643
88-89	3.7256344012266836E-4	6.025284745919165E-4	2.972473807986788E-4	6.908993175320643E-4	0.0012612929401457451	0.0024050928277460667
>>END_MODULE
