##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674222
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1357122
Total Bases	135.6 Mbp
Sequences flagged as poor quality	0
Sequence length	84-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.24548566746394	37.0	36.0	37.0	31.0	37.0
2	34.79491305866385	37.0	35.0	37.0	29.0	37.0
3	35.11008369181253	37.0	35.0	37.0	30.0	37.0
4	34.809653811521734	37.0	35.0	37.0	30.0	37.0
5	34.94494746971901	37.0	35.0	37.0	30.0	37.0
6	34.809919078756366	37.0	35.0	37.0	29.0	37.0
7	34.65017367635334	37.0	35.0	37.0	29.0	37.0
8	34.901888702710586	37.0	35.0	37.0	30.0	37.0
9	34.93131420756572	37.0	35.0	37.0	30.0	37.0
10-11	34.90887591535618	37.0	35.0	37.0	30.0	37.0
12-13	34.82872320985143	37.0	35.0	37.0	29.0	37.0
14-15	34.855869627049	37.0	35.0	37.0	29.5	37.0
16-17	34.53985677043037	37.0	35.0	37.0	28.5	37.0
18-19	33.67872600989446	37.0	34.5	37.0	26.5	37.0
20-21	33.81011987131592	37.0	34.5	37.0	26.5	37.0
22-23	34.51929266491885	37.0	35.0	37.0	28.5	37.0
24-25	34.75851581508516	37.0	35.0	37.0	29.0	37.0
26-27	34.33285953657814	37.0	35.0	37.0	28.0	37.0
28-29	34.5592463315752	37.0	35.0	37.0	28.5	37.0
30-31	34.71699965073147	37.0	35.0	37.0	29.0	37.0
32-33	33.815005209553746	37.0	34.0	37.0	27.0	37.0
34-35	33.46689354383762	37.0	34.0	37.0	25.0	37.0
36-37	33.4691906844042	37.0	34.0	37.0	24.5	37.0
38-39	34.57526884097376	37.0	35.0	37.0	28.5	37.0
40-41	34.49756617312224	37.0	35.0	37.0	28.5	37.0
42-43	34.70858367928602	37.0	35.0	37.0	29.0	37.0
44-45	34.62877803174659	37.0	35.0	37.0	29.0	37.0
46-47	34.02397684217041	37.0	34.5	37.0	27.0	37.0
48-49	34.62480528648125	37.0	35.0	37.0	29.0	37.0
50-51	34.65109253257998	37.0	35.0	37.0	29.0	37.0
52-53	33.44122341248613	37.0	34.0	37.0	24.5	37.0
54-55	34.16501906239822	37.0	34.5	37.0	27.5	37.0
56-57	34.34230894495852	37.0	34.5	37.0	28.0	37.0
58-59	32.739985425039166	37.0	33.5	37.0	21.5	37.0
60-61	34.549869503257625	37.0	35.0	37.0	28.5	37.0
62-63	34.38214950461344	37.0	34.5	37.0	28.0	37.0
64-65	34.549560024817225	37.0	35.0	37.0	28.0	37.0
66-67	34.540214512770405	37.0	35.0	37.0	28.0	37.0
68-69	34.465858264769125	37.0	34.5	37.0	28.0	37.0
70-71	34.51955866900691	37.0	35.0	37.0	28.0	37.0
72-73	33.914037205203364	37.0	34.0	37.0	26.5	37.0
74-75	33.49713400858582	37.0	34.0	37.0	26.0	37.0
76-77	34.33683780824421	37.0	34.5	37.0	27.5	37.0
78-79	34.17065378057389	37.0	34.0	37.0	27.0	37.0
80-81	33.829937175876594	37.0	34.0	37.0	26.5	37.0
82-83	34.3753406104978	37.0	34.0	37.0	28.0	37.0
84-85	33.745021200037726	37.0	34.0	37.0	26.0	37.0
86-87	34.16105233063227	37.0	34.0	37.0	27.5	37.0
88-89	34.26545220761613	37.0	34.0	37.0	27.5	37.0
90-91	34.31958879247195	37.0	34.0	37.0	27.5	37.0
92-93	33.79325354431961	37.0	34.0	37.0	26.0	37.0
94-95	33.561013906203385	37.0	33.5	37.0	25.5	37.0
96-97	34.324122421069774	37.0	34.0	37.0	27.5	37.0
98-99	34.19677294813363	37.0	34.0	37.0	27.5	37.0
100	34.925068132470145	37.0	35.0	37.0	29.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	11.0
21	139.0
22	566.0
23	1652.0
24	3196.0
25	5769.0
26	9537.0
27	15163.0
28	22745.0
29	32640.0
30	46278.0
31	65743.0
32	94154.0
33	140253.0
34	224444.0
35	370935.0
36	317041.0
37	6856.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	25.762545495544952	31.904766466642247	19.209418416051278	23.123269621761516
2	25.465765739212447	31.642153973119548	26.179114595614244	16.71296569205376
3	17.6426420341296	28.565028468353226	30.83358079345909	22.958748704058078
4	19.776920159725165	29.42274373331549	28.92772956774563	21.872606539213717
5	21.50057806364521	28.319223766263335	28.677704280471026	21.50249388962042
6	21.25519105976163	28.112073053541575	29.177388034989825	21.455347851706975
7	21.933950689804398	28.772137761604434	28.29023388064607	21.0036776679451
8	20.14208039236058	27.687328819359248	29.955663431136102	22.214927357144067
9	21.06803978769482	26.972234237194964	30.03515576931154	21.924570205798677
10-11	21.995299603314226	27.7274772044761	28.323187456963073	21.954035735246602
12-13	22.00810764906546	27.96523651116874	27.95186253765805	22.074793302107746
14-15	22.051193250375338	28.094870543156887	27.953909495343975	21.9000267111238
16-17	22.35534853964807	28.049775141085227	27.765290757922816	21.82958556134389
18-19	22.190791437526006	27.84317429179273	27.85163253594469	22.114401734736568
20-21	22.269064052681212	27.90578150701137	27.790236648097228	22.034917792210194
22-23	22.416761321985554	28.071349130775392	27.58227608155117	21.92961346568788
24-25	22.379177252872275	27.926103372455625	27.648528640230136	22.04619073444196
26-27	22.303749415920883	27.92032119143212	27.810239018568506	21.965690374078495
28-29	22.553605386483603	27.90899945741363	27.613759931070064	21.9236352250327
30-31	22.482027045000454	27.861294804357943	27.572408543605764	22.084269607035843
32-33	22.397553844591993	27.856341960570006	27.676208972381495	22.069895222456502
34-35	22.58853917288823	27.83737763333518	27.41077790331772	22.163305290458872
36-37	22.597995879420584	27.74215198732269	27.41865101983963	22.2412011134171
38-39	22.416128675249936	27.865053307263803	27.678828346921456	22.0399896705648
40-41	22.557517808927177	27.90014042775486	27.53124452755668	22.01109723576128
42-43	22.465434859258064	27.844469669588683	27.540699493004073	22.14939597814918
44-45	22.402745253502673	27.85747760307596	27.6189184927338	22.120858650687566
46-47	22.60741008705219	27.864362415756244	27.461838522556807	22.066388974634755
48-49	22.519747005481154	27.80010677143875	27.548431237240756	22.131714985839338
50-51	22.454165997279215	27.799709940293095	27.619638574993253	22.12648548743443
52-53	22.6609698023701	27.806585842214766	27.40087016035899	22.131574195056142
54-55	22.48912593456701	27.799069813118216	27.500997851039905	22.21080640127487
56-57	22.408667334724978	27.748420148214176	27.649618099913344	22.193294417147506
58-59	22.56909783966237	27.804964489614626	27.460577978144364	22.165359692578647
60-61	22.559997465181244	27.748380921362408	27.500830816322168	22.19079079713418
62-63	22.43296489972036	27.82327772895438	27.648550019138607	22.095207352186648
64-65	22.612143512157992	27.867643889245652	27.46940837211976	22.0508042264766
66-67	22.540249458587766	27.7616813511763	27.5297528035906	22.168316386645337
68-69	22.457560920970018	27.80909267769292	27.628842656322313	22.104503745014753
70-71	22.625596028027225	27.820315096281877	27.501107132883213	22.052981742807688
72-73	22.478789328509386	27.75136981693415	27.51070811873491	22.259132735821556
74-75	22.436185863186676	27.767237748811546	27.59324829962272	22.20332808837906
76-77	22.62441063199181	27.873076604530368	27.430754805161	22.071757958316816
78-79	22.530469186612436	27.731715712218392	27.508281178869765	22.22953392229941
80-81	22.496715978376844	27.721290737283876	27.59534874160776	22.18664454273152
82-83	22.643669267782272	27.7989625011974	27.441806485841237	22.115561745179093
84-85	22.498319916335944	27.764214180578904	27.50265187844193	22.23481402464322
86-87	22.51472171508027	27.73981563760543	27.60942885556083	22.13603379175347
88-89	22.633660856531872	27.803794101045355	27.45152366818122	22.111021374241556
90-91	22.51548149589125	27.763421226376167	27.536688418868415	22.184408858864167
92-93	22.46432006873558	27.804140945301043	27.634618078578967	22.09692090738441
94-95	22.659420677638686	27.841610124425987	27.397929264786836	22.101039933148492
96-97	22.564840670261617	27.816579372291166	27.459147779907628	22.159432177539585
98-99	22.473704313134796	27.75399924203984	27.635776758833185	22.136519685992177
100	22.82110744694439	27.396947234460335	27.27822515492346	22.503720163671815
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.5
8	2.5
9	4.0
10	8.0
11	11.0
12	15.5
13	24.5
14	42.5
15	75.5
16	132.0
17	226.0
18	378.0
19	576.0
20	916.5
21	1463.5
22	2188.5
23	3194.0
24	4446.0
25	5884.0
26	7666.0
27	9594.0
28	11653.5
29	13992.5
30	16541.5
31	19304.0
32	22191.5
33	25559.5
34	29216.0
35	33026.0
36	37191.5
37	41550.0
38	46004.5
39	49876.5
40	53541.0
41	56868.5
42	59347.5
43	61293.5
44	62420.0
45	62236.5
46	61193.0
47	59538.5
48	56859.0
49	53944.5
50	50729.0
51	46965.0
52	43121.5
53	38700.5
54	33993.5
55	29825.0
56	25868.5
57	21840.5
58	18153.0
59	14974.5
60	12280.5
61	10054.0
62	8141.5
63	6621.0
64	5367.0
65	4326.5
66	3539.5
67	2856.5
68	2273.5
69	1803.0
70	1413.5
71	1111.5
72	886.5
73	680.0
74	482.0
75	346.5
76	255.0
77	182.0
78	116.5
79	84.0
80	62.5
81	37.0
82	20.0
83	12.0
84	8.5
85	6.0
86	3.5
87	1.0
88	0.5
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008915926497396696
2	1.473706859073834E-4
3	7.36853429536917E-5
4	0.9072139424458524
5	3.6842671476845854E-4
6	0.08731713140012468
7	0.902498080496816
8	2.2105602886107514E-4
9	0.0014000215161201425
10-11	1.8421335738422927E-4
12-13	6.263254151063796E-4
14-15	7.000107580600712E-4
16-17	0.4869495889094717
18-19	2.864996662053964
20-21	2.3649679247702124
22-23	0.48691274623799485
24-25	7.368534295369171E-4
26-27	0.9671938116101574
28-29	0.4557438461685832
30-31	9.94752129874838E-4
32-33	2.377641803758247
34-35	2.8928128790189827
36-37	2.398531598485619
38-39	0.1305704277139417
40-41	0.4867285328806105
42-43	7.736961010137629E-4
44-45	0.0011789654872590672
46-47	1.4139480459383902
48-49	0.0012894935016896049
50-51	0.011789654872590673
52-53	2.8887601851565297
54-55	0.9566936502392563
56-57	0.47464413663620514
58-59	4.776578671630111
60-61	0.0016947628879349095
62-63	0.4751967767083578
64-65	7.736961010137629E-4
66-67	0.0016947628879349095
68-69	0.008694870468535622
70-71	9.579094583979923E-4
72-73	1.425958756839842
74-75	2.8056799609762426
76-77	0.3556054650945162
78-79	0.45441750999541675
80-81	1.4150901687541724
82-83	8.842241154443006E-4
84-85	1.4287961689502378
86-87	0.45246518180766493
88-89	0.004863239801933507
90-91	0.0011052825875991715
92-93	1.3744592851529587
94-95	1.547386144669312
96-97	5.89574408343657E-4
98-99	0.44988485903730924
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
84	1.0
85	0.0
86	0.0
87	1.0
88	0.0
89	0.0
90	2.0
91	1.0
92	3.0
93	11.0
94	16.0
95	68.0
96	216.0
97	1012.0
98	5334.0
99	50783.0
100	1299674.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.62019376007429
#Duplication Level	Percentage of total
1	97.3800263883682
2	2.2744229318568343
3	0.23571969253691188
4	0.06364049471048597
5	0.03125486399194391
6	0.008397225616131222
7	0.004970169027178334
8	0.0
9	0.0
>10	0.0015682338923333277
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	7.368534295369171E-5	0.0
5	0.0	0.0	0.0	0.0	1.4737068590738343E-4	0.0
6	0.0	0.0	0.0	0.0	1.4737068590738343E-4	0.0
7	0.0	0.0	0.0	0.0	2.2105602886107512E-4	0.0
8	0.0	0.0	0.0	0.0	2.2105602886107512E-4	7.368534295369171E-5
9	0.0	0.0	0.0	0.0	2.9474137181476686E-4	7.368534295369171E-5
10-11	0.0	0.0	0.0	0.0	2.9474137181476686E-4	7.368534295369171E-5
12-13	0.0	0.0	0.0	0.0	2.9474137181476686E-4	7.368534295369171E-5
14-15	0.0	0.0	0.0	0.0	2.9474137181476686E-4	7.368534295369171E-5
16-17	0.0	3.684267147684586E-5	0.0	0.0	3.315840432916127E-4	7.368534295369171E-5
18-19	0.0	7.368534295369171E-5	0.0	0.0	3.6842671476845854E-4	1.1052801443053757E-4
20-21	0.0	7.368534295369171E-5	7.368534295369171E-5	0.0	3.6842671476845854E-4	1.4737068590738343E-4
22-23	0.0	7.368534295369171E-5	7.368534295369171E-5	0.0	3.6842671476845854E-4	1.4737068590738343E-4
24-25	0.0	7.368534295369171E-5	7.368534295369171E-5	0.0	3.6842671476845854E-4	1.8421335738422927E-4
26-27	0.0	7.368534295369171E-5	7.368534295369171E-5	0.0	4.789547291989961E-4	2.9474137181476686E-4
28-29	0.0	7.368534295369171E-5	7.368534295369171E-5	0.0	5.157974006758419E-4	3.315840432916127E-4
30-31	0.0	1.1052801443053757E-4	7.368534295369171E-5	0.0	5.157974006758419E-4	3.6842671476845854E-4
32-33	0.0	1.8421335738422927E-4	7.368534295369171E-5	0.0	5.157974006758419E-4	3.6842671476845854E-4
34-35	0.0	2.2105602886107512E-4	7.368534295369171E-5	0.0	5.157974006758419E-4	3.6842671476845854E-4
36-37	0.0	2.2105602886107512E-4	7.368534295369171E-5	3.684267147684586E-5	5.157974006758419E-4	3.6842671476845854E-4
38-39	0.0	2.2105602886107512E-4	7.368534295369171E-5	1.1052801443053757E-4	5.157974006758419E-4	3.6842671476845854E-4
40-41	7.368534295369171E-5	2.2105602886107512E-4	7.368534295369171E-5	1.4737068590738343E-4	5.157974006758419E-4	4.052693862453044E-4
42-43	1.1052801443053757E-4	2.2105602886107512E-4	7.368534295369171E-5	1.4737068590738343E-4	5.157974006758419E-4	4.789547291989961E-4
44-45	1.4737068590738343E-4	2.2105602886107512E-4	1.1052801443053757E-4	1.4737068590738343E-4	5.157974006758419E-4	6.631680865832254E-4
46-47	1.4737068590738343E-4	2.2105602886107512E-4	1.4737068590738343E-4	1.4737068590738343E-4	5.526400721526878E-4	6.631680865832254E-4
48-49	1.4737068590738343E-4	2.2105602886107512E-4	1.4737068590738343E-4	1.4737068590738343E-4	5.894827436295337E-4	6.631680865832254E-4
50-51	1.4737068590738343E-4	2.2105602886107512E-4	1.4737068590738343E-4	1.4737068590738343E-4	5.894827436295337E-4	6.631680865832254E-4
52-53	1.8421335738422927E-4	2.2105602886107512E-4	1.4737068590738343E-4	1.4737068590738343E-4	5.894827436295337E-4	6.631680865832254E-4
54-55	2.2105602886107512E-4	2.2105602886107512E-4	1.4737068590738343E-4	1.4737068590738343E-4	5.894827436295337E-4	7.000107580600712E-4
56-57	2.2105602886107512E-4	2.2105602886107512E-4	1.4737068590738343E-4	1.4737068590738343E-4	5.894827436295337E-4	7.368534295369171E-4
58-59	2.2105602886107512E-4	2.2105602886107512E-4	1.4737068590738343E-4	1.4737068590738343E-4	6.631680865832254E-4	7.368534295369171E-4
60-61	2.2105602886107512E-4	2.2105602886107512E-4	1.4737068590738343E-4	1.4737068590738343E-4	6.631680865832254E-4	7.368534295369171E-4
62-63	2.2105602886107512E-4	2.2105602886107512E-4	1.4737068590738343E-4	1.4737068590738343E-4	6.631680865832254E-4	7.368534295369171E-4
64-65	2.2105602886107512E-4	2.2105602886107512E-4	1.4737068590738343E-4	1.4737068590738343E-4	7.000107580600712E-4	7.368534295369171E-4
66-67	2.2105602886107512E-4	2.9474137181476686E-4	1.4737068590738343E-4	1.4737068590738343E-4	8.105387724906088E-4	7.368534295369171E-4
68-69	2.2105602886107512E-4	2.9474137181476686E-4	1.4737068590738343E-4	1.4737068590738343E-4	8.105387724906088E-4	7.368534295369171E-4
70-71	2.2105602886107512E-4	2.9474137181476686E-4	1.4737068590738343E-4	1.4737068590738343E-4	8.842241154443005E-4	7.736961010137629E-4
72-73	2.2105602886107512E-4	2.9474137181476686E-4	1.4737068590738343E-4	1.4737068590738343E-4	8.842241154443005E-4	8.473814439674546E-4
74-75	2.2105602886107512E-4	2.9474137181476686E-4	1.4737068590738343E-4	1.4737068590738343E-4	8.842241154443005E-4	8.842241154443005E-4
76-77	2.2105602886107512E-4	2.9474137181476686E-4	1.4737068590738343E-4	1.4737068590738343E-4	9.210667869211464E-4	8.842241154443005E-4
78-79	2.9474137181476686E-4	2.9474137181476686E-4	1.4737068590738343E-4	1.4737068590738343E-4	9.579094583979923E-4	9.579094583979923E-4
80-81	2.9474137181476686E-4	3.315840432916127E-4	1.4737068590738343E-4	1.4737068590738343E-4	9.579094583979923E-4	0.0010315948013516838
82-83	2.9474137181476686E-4	3.6842671476845854E-4	1.4737068590738343E-4	1.4737068590738343E-4	0.0010315948013516838	0.0010315948013516838
84-85	2.9474137181476686E-4	3.6842671476845854E-4	1.4737068590738343E-4	1.4737068590738343E-4	0.0010315948013516838	0.0010684374728285298
86-87	3.315840432916127E-4	3.6842671476845854E-4	1.4737068590738343E-4	1.4737068590738343E-4	0.0010315948013516838	0.0011052801443053757
88-89	3.6842671476845854E-4	3.6842671476845854E-4	1.4737068590738343E-4	1.4737068590738343E-4	0.0010315948013516838	0.0011052801443053757
>>END_MODULE
