##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674223
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8428329
Total Bases	842.3 Mbp
Sequences flagged as poor quality	0
Sequence length	74-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.412137091468544	37.0	36.0	37.0	31.0	37.0
2	35.10093210647093	37.0	35.0	37.0	30.0	37.0
3	35.28133037996025	37.0	36.0	37.0	31.0	37.0
4	35.26757261136816	37.0	36.0	37.0	31.0	37.0
5	35.27766144392323	37.0	36.0	37.0	31.0	37.0
6	35.03473582960513	37.0	35.0	37.0	30.0	37.0
7	35.13681169778731	37.0	35.0	37.0	30.0	37.0
8	35.18626551004357	37.0	35.0	37.0	30.0	37.0
9	35.20539907732601	37.0	35.0	37.0	31.0	37.0
10-11	35.18756493724912	37.0	35.0	37.0	30.5	37.0
12-13	35.10898512623321	37.0	35.0	37.0	30.0	37.0
14-15	35.11995307729445	37.0	35.0	37.0	30.0	37.0
16-17	35.03850306507968	37.0	35.0	37.0	30.0	37.0
18-19	34.893765715600324	37.0	35.0	37.0	30.0	37.0
20-21	34.92997164681161	37.0	35.0	37.0	30.0	37.0
22-23	35.03745635700742	37.0	35.0	37.0	30.0	37.0
24-25	35.071986214586545	37.0	35.0	37.0	30.0	37.0
26-27	34.97033557897419	37.0	35.0	37.0	30.0	37.0
28-29	35.037040972178474	37.0	35.0	37.0	30.0	37.0
30-31	35.049086183038185	37.0	35.0	37.0	30.0	37.0
32-33	34.91124729468914	37.0	35.0	37.0	30.0	37.0
34-35	34.84258676897876	37.0	35.0	37.0	29.5	37.0
36-37	34.8246095400405	37.0	35.0	37.0	29.5	37.0
38-39	34.89316251180988	37.0	35.0	37.0	29.5	37.0
40-41	34.98612388054619	37.0	35.0	37.0	30.0	37.0
42-43	35.01715239165438	37.0	35.0	37.0	30.0	37.0
44-45	34.98689556375884	37.0	35.0	37.0	30.0	37.0
46-47	34.889271823631944	37.0	35.0	37.0	29.5	37.0
48-49	34.99675333034579	37.0	35.0	37.0	30.0	37.0
50-51	34.95475817329864	37.0	35.0	37.0	30.0	37.0
52-53	34.73686676208297	37.0	35.0	37.0	29.5	37.0
54-55	34.84484409661749	37.0	35.0	37.0	29.5	37.0
56-57	34.90075132330501	37.0	35.0	37.0	30.0	37.0
58-59	34.65466138068412	37.0	35.0	37.0	29.0	37.0
60-61	34.90770815899569	37.0	35.0	37.0	30.0	37.0
62-63	34.88758863115097	37.0	35.0	37.0	29.5	37.0
64-65	34.914593034989494	37.0	35.0	37.0	30.0	37.0
66-67	34.87601130663029	37.0	35.0	37.0	29.0	37.0
68-69	34.87705475189685	37.0	35.0	37.0	29.5	37.0
70-71	34.87074751116147	37.0	35.0	37.0	29.0	37.0
72-73	34.75445844603361	37.0	35.0	37.0	29.0	37.0
74-75	34.64524960548116	37.0	35.0	37.0	29.0	37.0
76-77	34.80197816770027	37.0	35.0	37.0	29.0	37.0
78-79	34.750829547252636	37.0	35.0	37.0	29.0	37.0
80-81	34.681199531853	37.0	35.0	37.0	29.0	37.0
82-83	34.692460603313236	37.0	35.0	37.0	29.0	37.0
84-85	34.515484784520424	37.0	35.0	37.0	28.5	37.0
86-87	34.580970677966334	37.0	35.0	37.0	28.5	37.0
88-89	34.525020518258046	37.0	35.0	37.0	28.5	37.0
90-91	34.4868949231092	37.0	35.0	37.0	28.0	37.0
92-93	34.41830196358772	37.0	35.0	37.0	28.0	37.0
94-95	34.19048740727685	37.0	34.0	37.0	27.0	37.0
96-97	34.45566346714537	37.0	35.0	37.0	28.0	37.0
98-99	34.44118206657001	37.0	35.0	37.0	28.0	37.0
100	35.05513933147589	37.0	35.0	37.0	30.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	37.0
21	525.0
22	2327.0
23	6529.0
24	13933.0
25	25590.0
26	43848.0
27	70866.0
28	107552.0
29	155168.0
30	217903.0
31	301605.0
32	427684.0
33	633450.0
34	1024693.0
35	1947831.0
36	3298257.0
37	150530.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	24.213074715628345	29.554293989690507	18.680313736196883	27.552317558484262
2	27.16836094537584	30.26151164883599	25.465507915209617	17.10461949057855
3	17.721797734092	28.09183466078238	31.331851266699502	22.854516338426123
4	18.996961226562483	28.182933709500197	30.342816269026784	22.477288794910532
5	21.545079542222737	27.21275285820103	28.812709480486614	22.429458119089624
6	21.649412591040722	28.00304493478214	29.226808646321288	21.12073382785585
7	22.496402192257055	28.77166317439145	27.915424135023343	20.81651049832815
8	20.255119717140076	26.73886476353195	29.89963456016706	23.106380959160912
9	21.41581664434085	26.171345991616253	30.17706013495155	22.23577722909135
10-11	22.741653666313233	26.551373696926778	27.886690981979573	22.820281654780416
12-13	22.88827834804904	27.21833609283809	27.264362138842763	22.6290234202701
14-15	23.070607378012337	27.27831273878798	27.062963789600435	22.58811609359925
16-17	23.068796545077646	27.190509650325072	27.124934283626967	22.615759520970315
18-19	22.986043500492993	27.2782514690864	27.165487680451843	22.570217349968765
20-21	23.167429201739083	27.17679640983214	27.01918221692377	22.636592171505004
22-23	23.098708959820744	27.206401629187845	26.994810003281742	22.700079407709673
24-25	23.027717980900423	27.375201278691797	27.10073909909369	22.49634164131409
26-27	23.191867742319978	27.224604603479914	26.999843354345256	22.58368429985486
28-29	23.174391542997512	27.070997706534346	27.0128225714775	22.741788178990642
30-31	23.097206409080272	27.243366746192464	27.046517847269648	22.612908997457613
32-33	23.187204369820332	27.186487654519414	26.924487733096175	22.70182024256408
34-35	23.170162578610956	27.12704712558881	26.95983501945377	22.742955276346464
36-37	23.07660986583603	27.29810965111701	27.01280182547481	22.61247865757215
38-39	23.25960960338173	27.121729665025423	26.925506892487554	22.69315383910529
40-41	23.240760815232452	27.039600899228173	26.90228148159406	22.81735680394532
42-43	23.09612582351456	27.264282760370545	26.956028936622488	22.68356247949241
44-45	23.227239033858954	27.179958882440026	26.87340733228547	22.719394751415546
46-47	23.16008113825627	27.100371380384676	26.94122873907336	22.798318742285694
48-49	23.122042387911517	27.259970394810217	26.99808928351929	22.61989793375898
50-51	23.24661384337534	27.146348935321353	26.86699620864448	22.74004101265883
52-53	23.1932854591651	27.046992805039793	26.905947660038265	22.853774075756846
54-55	23.096744185382896	27.235645340518538	26.968910949902114	22.69869952419645
56-57	23.24000323189263	27.14602805798101	26.887209499959603	22.72675921016676
58-59	23.203887073135636	27.07817205496314	26.899890497326584	22.818050374574643
60-61	23.102081312091453	27.259312879996934	26.953333887821508	22.68527192009011
62-63	23.249025181553332	27.148435505736117	26.81137782493137	22.79116148777918
64-65	23.211600211718032	27.079618864831133	26.847866879144057	22.860914044306778
66-67	23.10417236679961	27.27235082935006	26.930366582717998	22.693110221132333
68-69	23.218175555573747	27.170364480014392	26.849633410373336	22.761826554038525
70-71	23.22442358526306	27.08887940492602	26.86239738840613	22.82429962140479
72-73	23.109107174279725	27.261290913242114	26.902165862721656	22.72743604975651
74-75	23.249956022863458	27.16566594448126	26.797659778078454	22.78671825457683
76-77	23.194664301998515	27.082418537953696	26.838544328328002	22.88437283171979
78-79	23.101865642536666	27.28681928492706	26.900959991280903	22.710355081255372
80-81	23.239579911022037	27.1699383046252	26.815562459010284	22.77491932534248
82-83	23.240882307365748	27.08198715403835	26.80349859886614	22.87363193972977
84-85	23.12587542554665	27.26662338818183	26.849387779190252	22.758113407081268
86-87	23.259727577373905	27.196837071798114	26.72977682003425	22.813658530793727
88-89	23.230810952461837	27.092645574125473	26.808645528798426	22.867897944614267
90-91	23.17223617715535	27.27236805171842	26.86013173928885	22.695264031837382
92-93	23.280535513384624	27.18778781597185	26.74119373788542	22.790482932758106
94-95	23.27469941885402	27.132017009278492	26.72916567657096	22.86411789529653
96-97	23.144505038375794	27.5049883277672	26.701548300709206	22.648958333147807
98-99	23.264716658786867	27.336448040792394	26.676256406611014	22.72257889380972
100	23.46475345019367	26.8731634345099	26.580739655622025	23.0813434596744
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	3.0
2	4.5
3	3.5
4	9.5
5	19.0
6	21.0
7	27.5
8	54.0
9	100.0
10	177.5
11	321.0
12	524.5
13	889.0
14	1416.5
15	2185.0
16	3278.5
17	4686.0
18	6697.5
19	9402.0
20	12691.5
21	16814.0
22	22071.5
23	28130.5
24	35744.5
25	44939.5
26	54381.5
27	64542.5
28	75941.0
29	88209.0
30	101746.0
31	116119.0
32	130741.5
33	147100.0
34	164966.0
35	182960.0
36	201611.5
37	219777.5
38	238465.0
39	257418.0
40	274997.0
41	290538.5
42	302926.0
43	313564.0
44	321083.0
45	324253.5
46	323533.0
47	319648.0
48	312762.0
49	302301.0
50	289593.0
51	274817.0
52	258999.0
53	242984.0
54	227096.0
55	211168.5
56	194776.5
57	180330.5
58	166336.5
59	152270.5
60	138736.0
61	124855.0
62	111911.5
63	99437.0
64	86598.5
65	74398.0
66	62843.5
67	51749.0
68	41567.0
69	32847.5
70	25298.5
71	18796.0
72	13805.5
73	9812.5
74	6767.5
75	4605.0
76	3022.0
77	1910.0
78	1242.0
79	814.0
80	510.0
81	313.0
82	175.0
83	110.5
84	81.0
85	51.5
86	32.5
87	27.0
88	17.0
89	12.0
90	9.5
91	5.0
92	2.0
93	3.5
94	4.0
95	1.5
96	0.5
97	0.5
98	1.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04809968856222865
2	7.356143786033981E-4
3	9.491798433592234E-5
4	0.12799690187699125
5	4.5086042559563114E-4
6	0.20149901599712117
7	0.1269883982934221
8	0.0015305524974167477
9	0.0014119050169968449
10-11	3.856043113646845E-4
12-13	0.07028676740075049
14-15	0.007261225801698058
16-17	0.07974890396423776
18-19	0.4114338678520974
20-21	0.3368105350419994
22-23	0.07086814005480802
24-25	3.26280571154733E-4
26-27	0.20913398136214187
28-29	0.06509594013237974
30-31	4.389956775536408E-4
32-33	0.34287935366547745
34-35	0.4164170620297333
36-37	0.3438403982568787
38-39	0.16809975025891846
40-41	0.0711291645117318
42-43	5.635755319945389E-4
44-45	6.703582643724515E-4
46-47	0.20178376995012892
48-49	9.135855992332525E-4
50-51	0.07758358744657452
52-53	0.519432736904314
54-55	0.21018401156385805
56-57	0.07141985083876057
58-59	0.6798559951800648
60-61	0.0016136057337106798
62-63	0.06804433002081432
64-65	0.0016373352297946605
66-67	0.0019339539308444178
68-69	0.00284753953007767
70-71	7.830733707713593E-4
72-73	0.27666219484312965
74-75	0.5473327893345388
76-77	0.05111930029738417
78-79	0.06439601550321467
80-81	0.20031935296024392
82-83	5.991772051159767E-4
84-85	0.28061369927117324
86-87	0.0643687707641196
88-89	0.0021120533281599892
90-91	0.002717276676492964
92-93	0.1995161938478383
94-95	0.36002304062010054
96-97	0.0013535937825875477
98-99	0.06486486486486487
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
74	3.0
75	0.0
76	5.0
77	1.0
78	7.0
79	11.0
80	20.0
81	46.0
82	23.0
83	52.0
84	53.0
85	67.0
86	82.0
87	81.0
88	121.0
89	129.0
90	145.0
91	196.0
92	303.0
93	502.0
94	987.0
95	1452.0
96	4037.0
97	10276.0
98	39960.0
99	309208.0
100	8060562.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.25092967735179
#Duplication Level	Percentage of total
1	95.05855969449453
2	3.767461221589962
3	0.5967580629443321
4	0.23503594491425256
5	0.13203311624784417
6	0.06383377766328763
7	0.027962960653798465
8	0.031253309268535236
9	0.019910334701120993
>10	0.056953582223284886
>50	0.010237995299052935
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	1.1864748041990293E-5	2.3729496083980585E-5	1.1864748041990293E-5	0.0
3	2.3729496083980585E-5	0.0	1.1864748041990293E-5	2.3729496083980585E-5	1.1864748041990293E-5	1.1864748041990293E-5
4	2.3729496083980585E-5	0.0	2.3729496083980585E-5	2.3729496083980585E-5	2.3729496083980585E-5	1.1864748041990293E-5
5	2.3729496083980585E-5	0.0	3.5594244125970875E-5	2.3729496083980585E-5	3.5594244125970875E-5	2.3729496083980585E-5
6	2.3729496083980585E-5	1.1864748041990293E-5	3.5594244125970875E-5	2.3729496083980585E-5	7.118848825194175E-5	4.745899216796117E-5
7	2.3729496083980585E-5	1.1864748041990293E-5	3.5594244125970875E-5	2.3729496083980585E-5	7.118848825194175E-5	5.932374020995146E-5
8	3.5594244125970875E-5	1.1864748041990293E-5	4.745899216796117E-5	2.3729496083980585E-5	9.491798433592234E-5	8.305323629393205E-5
9	3.5594244125970875E-5	1.1864748041990293E-5	4.745899216796117E-5	2.3729496083980585E-5	1.1864748041990292E-4	1.8983596867184468E-4
10-11	3.5594244125970875E-5	1.1864748041990293E-5	5.932374020995146E-5	2.966187010497573E-5	1.423769765038835E-4	2.313625868188107E-4
12-13	4.152661814696602E-5	1.7797122062985437E-5	7.118848825194175E-5	3.5594244125970875E-5	1.542417245458738E-4	2.788215789867719E-4
14-15	4.745899216796117E-5	3.5594244125970875E-5	7.71208622729369E-5	4.152661814696602E-5	1.8390359465084952E-4	3.084834490917476E-4
16-17	5.339136618895632E-5	3.5594244125970875E-5	8.89856103149272E-5	5.339136618895632E-5	1.9576834269283984E-4	3.737395633226942E-4
18-19	5.932374020995146E-5	3.5594244125970875E-5	1.1864748041990292E-4	7.118848825194175E-5	2.1949783877682044E-4	3.8560431136468447E-4
20-21	5.932374020995146E-5	4.152661814696602E-5	1.1864748041990292E-4	1.0678273237791264E-4	2.610244569237864E-4	4.2713092951165055E-4
22-23	6.52561142309466E-5	5.932374020995146E-5	1.4830935052487866E-4	1.0678273237791264E-4	3.084834490917476E-4	4.7458992167961173E-4
24-25	8.305323629393205E-5	7.118848825194175E-5	1.542417245458738E-4	1.0678273237791264E-4	3.500100672387136E-4	5.101841658055826E-4
26-27	9.491798433592234E-5	8.89856103149272E-5	1.661064725878641E-4	1.1271510639890777E-4	3.6780718930169904E-4	5.576431579735438E-4
28-29	1.1271510639890777E-4	1.0678273237791264E-4	1.661064725878641E-4	1.1864748041990292E-4	3.9153668538567963E-4	6.406963942674757E-4
30-31	1.1864748041990292E-4	1.0678273237791264E-4	1.661064725878641E-4	1.2457985444089807E-4	4.0340143342766996E-4	6.88155386435437E-4
32-33	1.1864748041990292E-4	1.0678273237791264E-4	1.8390359465084952E-4	1.3051222846189323E-4	4.211985554906554E-4	7.652762487083738E-4
34-35	1.2457985444089807E-4	1.1271510639890777E-4	2.0170071671383498E-4	1.3051222846189323E-4	4.3306330353264566E-4	8.601942330442962E-4
36-37	1.3051222846189323E-4	1.1864748041990292E-4	2.1356546475582527E-4	1.3051222846189323E-4	4.5086042559563114E-4	9.966388355271845E-4
38-39	1.4830935052487866E-4	1.3051222846189323E-4	2.313625868188107E-4	1.3051222846189323E-4	4.8052229570060684E-4	0.0010322330796531554
40-41	1.661064725878641E-4	1.542417245458738E-4	2.4322733486080097E-4	1.3051222846189323E-4	4.86454669721602E-4	0.0010737596978001216
42-43	1.661064725878641E-4	1.542417245458738E-4	2.4915970888179614E-4	1.6017409856686893E-4	5.220489138475729E-4	0.0011330834380100729
44-45	1.7203884660885925E-4	1.542417245458738E-4	2.6695683094478157E-4	1.779712206298544E-4	5.517107839525487E-4	0.0011508805600730584
46-47	1.779712206298544E-4	1.661064725878641E-4	2.84753953007767E-4	1.8390359465084952E-4	5.69507906015534E-4	0.0011568129340940535
48-49	1.779712206298544E-4	1.779712206298544E-4	2.84753953007767E-4	1.8983596867184468E-4	6.110345241625001E-4	0.0011924071782200244
50-51	1.779712206298544E-4	1.8983596867184468E-4	2.84753953007767E-4	1.8983596867184468E-4	6.288316462254855E-4	0.001257663292450971
52-53	1.8983596867184468E-4	1.9576834269283984E-4	3.0255107507075243E-4	2.1949783877682044E-4	6.466287682884709E-4	0.0012873251625559468
54-55	1.8983596867184468E-4	2.076330907348301E-4	3.1441582311274275E-4	2.2543021279781557E-4	7.000201344774272E-4	0.0013525812767868934
56-57	1.8983596867184468E-4	2.2543021279781557E-4	3.203481971337379E-4	2.2543021279781557E-4	7.534115006663836E-4	0.0014356345130808253
58-59	1.8983596867184468E-4	2.2543021279781557E-4	3.203481971337379E-4	2.2543021279781557E-4	8.12735240876335E-4	0.0014890258792697817
60-61	1.8983596867184468E-4	2.3729496083980584E-4	3.203481971337379E-4	2.313625868188107E-4	8.186676148973301E-4	0.0015602143675217234
62-63	1.8983596867184468E-4	2.3729496083980584E-4	3.203481971337379E-4	2.3729496083980584E-4	8.483294850023059E-4	0.0016551323518576458
64-65	1.8983596867184468E-4	2.3729496083980584E-4	3.203481971337379E-4	2.4915970888179614E-4	8.720589810862864E-4	0.0016847942219626216
66-67	1.8983596867184468E-4	2.3729496083980584E-4	3.262805711547331E-4	2.7288920496577673E-4	9.135855992332526E-4	0.0017322532141305826
68-69	1.8983596867184468E-4	2.3729496083980584E-4	3.322129451757282E-4	2.7288920496577673E-4	9.729093394432039E-4	0.0018093740764035197
70-71	2.0170071671383498E-4	2.4322733486080097E-4	3.559424412597088E-4	2.7288920496577673E-4	0.001055962575737136	0.0018449683205294903
72-73	2.0170071671383498E-4	2.7288920496577673E-4	3.559424412597088E-4	2.7288920496577673E-4	0.0010856244458421118	0.0019161568087814323
74-75	2.0170071671383498E-4	2.84753953007767E-4	3.6780718930169904E-4	2.7288920496577673E-4	0.0011449481860520633	0.0019636158009493933
76-77	2.0170071671383498E-4	3.0255107507075243E-4	3.7967193734368937E-4	2.7288920496577673E-4	0.0011864748041990293	0.002034804289201335
78-79	2.0170071671383498E-4	3.084834490917476E-4	3.7967193734368937E-4	2.9068632702876216E-4	0.0012280014223459952	0.002076330907348301
80-81	2.0170071671383498E-4	3.084834490917476E-4	3.915366853856797E-4	3.1441582311274275E-4	0.0012813927885349514	0.0021297222735372576
82-83	2.0170071671383498E-4	3.262805711547331E-4	4.0340143342766996E-4	3.203481971337379E-4	0.001299189910597937	0.002189046013747209
84-85	2.0170071671383498E-4	3.559424412597088E-4	4.0340143342766996E-4	3.203481971337379E-4	0.0013169870326609225	0.002355152486335073
86-87	2.1949783877682044E-4	3.737395633226942E-4	4.152661814696602E-4	3.203481971337379E-4	0.0013585136508078885	0.002467867592733981
88-89	2.3729496083980584E-4	3.915366853856797E-4	4.152661814696602E-4	3.440776932177185E-4	0.0014059726429758498	0.0026221093172798544
>>END_MODULE
