##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674225
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16949583
Total Bases	1.6 Gbp
Sequences flagged as poor quality	0
Sequence length	66-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.62539774577345	37.0	36.0	37.0	32.0	37.0
2	35.03829675337735	37.0	35.0	37.0	30.0	37.0
3	35.44769909678604	37.0	36.0	37.0	31.0	37.0
4	35.50927364997711	37.0	36.0	37.0	32.0	37.0
5	35.406829890741264	37.0	36.0	37.0	31.0	37.0
6	35.42969652999723	37.0	36.0	37.0	31.0	37.0
7	35.46362922320862	37.0	36.0	37.0	31.0	37.0
8	35.37978574458145	37.0	36.0	37.0	31.0	37.0
9	35.43189717410747	37.0	36.0	37.0	31.0	37.0
10-11	35.42958030294905	37.0	36.0	37.0	31.0	37.0
12-13	35.410985155210014	37.0	36.0	37.0	31.0	37.0
14-15	35.446821317079014	37.0	36.0	37.0	31.0	37.0
16-17	35.41836344882349	37.0	36.0	37.0	31.0	37.0
18-19	35.381053120893895	37.0	36.0	37.0	31.0	37.0
20-21	35.36266098699892	37.0	36.0	37.0	31.0	37.0
22-23	35.34609795415026	37.0	36.0	37.0	31.0	37.0
24-25	35.35052815163653	37.0	36.0	37.0	31.0	37.0
26-27	35.361671110138815	37.0	36.0	37.0	31.0	37.0
28-29	35.33163007609096	37.0	36.0	37.0	31.0	37.0
30-31	35.20147430765701	37.0	36.0	37.0	31.0	37.0
32-33	35.34169778690131	37.0	36.0	37.0	31.0	37.0
34-35	35.21604000523199	37.0	36.0	37.0	31.0	37.0
36-37	35.270180186733796	37.0	36.0	37.0	31.0	37.0
38-39	35.267566995601015	37.0	36.0	37.0	31.0	37.0
40-41	35.23508209612	37.0	36.0	37.0	31.0	37.0
42-43	35.221123611123645	37.0	36.0	37.0	31.0	37.0
44-45	35.1806882505605	37.0	36.0	37.0	30.5	37.0
46-47	35.175768837498836	37.0	36.0	37.0	31.0	37.0
48-49	35.15365569760625	37.0	36.0	37.0	30.5	37.0
50-51	35.22836774214446	37.0	36.0	37.0	31.0	37.0
52-53	35.13014181528832	37.0	36.0	37.0	30.0	37.0
54-55	35.02878604742075	37.0	36.0	37.0	30.0	37.0
56-57	35.167663239856694	37.0	36.0	37.0	30.5	37.0
58-59	35.13442026862844	37.0	36.0	37.0	30.0	37.0
60-61	35.14859731357403	37.0	36.0	37.0	30.5	37.5
62-63	35.12012764561818	37.0	36.0	37.0	30.0	38.0
64-65	35.099107216974005	37.0	35.5	37.0	30.0	38.0
66-67	35.12884382198163	37.0	36.0	37.0	30.0	38.0
68-69	35.08150953221147	37.0	35.5	37.0	30.0	38.0
70-71	35.0690693220717	37.0	35.0	37.0	30.0	38.0
72-73	35.06039613742237	37.0	35.0	37.0	30.0	38.0
74-75	34.920232838295	37.0	35.0	37.0	30.0	38.0
76-77	34.88759544573268	37.0	35.0	37.0	29.5	38.0
78-79	34.95494597433799	37.0	35.0	37.0	30.0	38.0
80-81	34.92756822006412	37.0	35.0	37.0	30.0	38.0
82-83	34.82190104834801	37.0	35.0	37.0	29.0	38.0
84-85	34.71962022263554	37.0	35.0	37.0	29.0	37.5
86-87	34.732553297272574	37.0	35.0	37.0	29.0	37.5
88-89	34.67858329451009	37.0	35.0	37.0	29.0	38.0
90-91	34.772599443070874	37.0	35.0	37.0	29.0	38.0
92-93	34.75533221294662	37.0	35.0	37.0	29.0	38.0
94-95	34.72306918009301	37.0	35.0	37.0	29.0	38.0
96-97	34.637935235075	37.0	35.0	37.0	29.0	38.0
98-99	34.728648157510705	37.0	35.0	37.0	29.0	38.0
100	35.17627827280093	37.0	35.0	37.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	101.0
21	1204.0
22	4098.0
23	10124.0
24	20518.0
25	38612.0
26	66237.0
27	106439.0
28	163089.0
29	236261.0
30	335850.0
31	473609.0
32	690012.0
33	1081631.0
34	1907295.0
35	4018734.0
36	7420995.0
37	374771.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.30064913432432	26.11909202149046	19.3031875165877	25.27707132759753
2	28.290678057572922	30.477804805891058	24.123386898127684	17.108130238408336
3	18.823735946558212	26.122933891188392	31.622490843152494	23.430839319100897
4	21.779119260146473	28.012239246807958	27.301105306975597	22.90753618606997
5	22.782778712624875	26.868009676627302	27.598924979322547	22.750286631425272
6	22.282330405801964	27.41065602212845	27.517110456053324	22.789903116016266
7	23.191979916397777	27.661620967023957	26.815539457716543	22.33085965886172
8	21.71672459674106	26.82924925816199	27.961838871424792	23.492187273672155
9	22.051981554715024	26.099825481661696	28.47270439525064	23.375488568372642
10-11	23.083941064271112	26.64566039225736	27.033723858641682	23.236674684829843
12-13	23.026324417961252	26.825392924377766	26.880722362427285	23.2675602952337
14-15	22.998258445341786	27.034942836477388	26.866687393978882	23.100111324201944
16-17	23.314561758777334	26.944845610986885	26.660774158592133	23.07981847164365
18-19	23.157537883805446	26.805217017412254	26.771218579114976	23.266026519667324
20-21	23.110358928138673	26.97466497020707	26.820294710512478	23.094681391141783
22-23	23.3432154368079	27.04532333987722	26.57725526461741	23.034205958697466
24-25	23.181752317622554	26.93280172965068	26.766667213835127	23.118778738891642
26-27	23.112578468250618	27.023413877301138	26.879724687390617	22.984282967057624
28-29	23.370860790287914	26.95225442263634	26.628628451309666	23.04825633576608
30-31	23.233420135030432	26.856570755496744	26.72739806383899	23.182611045633834
32-33	23.14196772247049	27.026286166161857	26.797519655242112	23.034226456125545
34-35	23.393699711107875	27.024189985829715	26.547613163605266	23.03449713945714
36-37	23.257711520870362	26.903877598578102	26.71222864850807	23.12618223204347
38-39	23.206895538810286	26.96987253680411	26.780635797066772	23.04259612731883
40-41	23.437837295409167	26.955059744729077	26.528073455690617	23.079029504171135
42-43	23.26811391490469	26.893659518038803	26.650563514454266	23.187663052602243
44-45	23.19759977861921	27.014717274346495	26.75378563806899	23.0338973089653
46-47	23.40930212207601	26.97707633100132	26.573116701751275	23.040504845171395
48-49	23.285487926634975	26.866519856550873	26.69600802315541	23.15198419365874
50-51	23.19534835848371	26.940568260123282	26.806938558134185	23.05714482325882
52-53	23.43218690415482	26.966425978736357	26.53723002887653	23.064157088232296
54-55	23.276652795946514	26.86743892838328	26.668768978519132	23.187139297151077
56-57	23.18122864192766	26.998870822634952	26.791682261115746	23.028218274321635
58-59	23.431305485338495	26.94894957880288	26.569564994415064	23.050179941443567
60-61	23.300189720524585	26.84990645312165	26.693103167076337	23.156800659277422
62-63	23.197630133338816	26.974317919407003	26.77800836884932	23.050043578404864
64-65	23.444685229102525	26.97312875109583	26.520069228329042	23.062116791472604
66-67	23.290387542132727	26.873613080832648	26.706571118943856	23.129428258090766
68-69	23.20046414499401	26.994882528129803	26.794382492593442	23.01027083428274
70-71	23.439440505019064	26.947505391264247	26.57011172881797	23.04294237489872
72-73	23.295455752566475	26.861911942547394	26.679126762139315	23.163505542746815
74-75	23.224982067986364	27.001406461440624	26.742593893502324	23.031017577070685
76-77	23.442090927650344	26.973963381163056	26.545222668134492	23.038723023052103
78-79	23.298291844689853	26.896111039623555	26.678391609064118	23.127205506622474
80-81	23.244681105330848	26.981682964364285	26.76199644882772	23.01163948147715
82-83	23.472993328653917	26.94796913266348	26.527931057422833	23.05110648125977
84-85	23.321378908609734	26.868161595975472	26.660432227614717	23.15002726780008
86-87	23.243754168410753	26.991683018748848	26.75127468830045	23.013288124539947
88-89	23.47921771432678	26.959975405268	26.50053428091613	23.06027259948909
90-91	23.358833884095525	26.83811440252064	26.67997669151848	23.123075021865354
92-93	23.248806839056748	26.952262411095194	26.75729975661529	23.04163099323277
94-95	23.477716349501836	26.919206671413846	26.53499140607606	23.06808557300826
96-97	23.350857927872354	26.855636984019032	26.651957817824858	23.141547270283755
98-99	23.238889606356498	26.962254509185275	26.753235676982378	23.04562020747585
100	23.869644446109636	26.349303371822764	26.270592411579095	23.510459770488502
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	0.5
7	0.5
8	1.5
9	9.0
10	24.5
11	48.5
12	94.0
13	277.0
14	924.5
15	1454.5
16	2109.0
17	3701.5
18	5509.0
19	7737.5
20	11117.0
21	15865.5
22	22423.0
23	31131.5
24	42690.5
25	57519.0
26	76436.5
27	97802.5
28	122973.0
29	155101.5
30	191681.0
31	230905.0
32	268903.0
33	306266.5
34	343459.5
35	378337.0
36	411010.0
37	443646.5
38	474144.0
39	501885.5
40	530902.5
41	558763.5
42	582400.5
43	603004.0
44	622183.5
45	634768.5
46	640193.5
47	641004.5
48	635823.0
49	624663.0
50	607162.0
51	584632.5
52	558785.5
53	530652.0
54	499261.5
55	465243.0
56	431539.5
57	398425.5
58	365135.0
59	331145.0
60	298124.5
61	266004.5
62	235219.0
63	206569.0
64	179142.0
65	152887.5
66	128274.0
67	105661.0
68	85426.0
69	67551.0
70	52073.5
71	38937.5
72	28107.5
73	19795.0
74	13708.5
75	9152.5
76	5883.0
77	3735.5
78	2292.5
79	1381.0
80	847.5
81	485.0
82	258.0
83	149.5
84	78.0
85	35.5
86	20.5
87	12.0
88	9.5
89	6.0
90	3.0
91	1.5
92	1.5
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010507633137641203
2	0.40836992862892263
3	0.05299245415064193
4	0.001026573928101948
5	0.11389070751770117
6	0.058650410455525666
7	2.0059490549118524E-4
8	0.209521378785543
9	0.09848029889584894
10-11	0.10511468040246182
12-13	0.13445168533054766
14-15	0.05293345564902688
16-17	0.02892401541678046
18-19	0.05368273661953807
20-21	0.16095971210619162
22-23	0.1438029478365338
24-25	0.08206101589637928
26-27	0.08301679162254316
28-29	0.08249760480833068
30-31	0.3184975111187101
32-33	0.02571154700384074
34-35	0.2083296090529189
36-37	0.08372772356700456
38-39	0.07743848329483975
40-41	0.05167088771446471
42-43	6.283340422003303E-4
44-45	0.11491728144580311
46-47	0.08174832383781949
48-49	0.08319673705246908
50-51	0.03168809521744576
52-53	0.05213107602706214
54-55	0.3273413865108068
56-57	0.08143268185417894
58-59	0.028932865192022716
60-61	0.05080655966580417
62-63	0.11315322624751299
64-65	0.10834484836588605
66-67	0.04738169804477485
68-69	0.054196026781073416
70-71	0.050246082793791946
72-73	0.026841377019574675
74-75	0.26814537438683195
76-77	0.26931949282611656
78-79	0.0013333677098341708
80-81	0.027900435858024836
82-83	0.27392778801076606
84-85	0.05427885365775017
86-87	0.15819070308886782
88-89	0.12149971839747692
90-91	0.05146808683619383
92-93	0.0013393030455751257
94-95	8.614353253091025E-4
96-97	0.13206200198275947
98-99	0.0015772858993451898
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
66	1.0
67	0.0
68	0.0
69	2.0
70	0.0
71	2.0
72	1.0
73	1.0
74	3.0
75	1.0
76	1.0
77	5.0
78	6.0
79	2.0
80	4.0
81	9.0
82	10.0
83	18.0
84	13.0
85	17.0
86	16.0
87	37.0
88	24.0
89	44.0
90	56.0
91	110.0
92	172.0
93	300.0
94	541.0
95	1381.0
96	3470.0
97	13004.0
98	68439.0
99	554126.0
100	1.6307767E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.50503260071416
#Duplication Level	Percentage of total
1	83.66451548598916
2	8.73222010158377
3	2.050213781370206
4	0.9244202033298772
5	0.7120294029620484
6	0.6037204147828048
7	0.5526727094070183
8	0.4707489297692478
9	0.40162144003765615
>10	1.8313186753644022
>50	0.029146708376653008
>100	0.01226364994667453
>500	0.0
>1k	0.01510849708044438
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	5.899850161505448E-6	0.0	0.0	1.1799700323010897E-5
2	0.0	0.0	5.899850161505448E-6	5.899850161505448E-6	1.1799700323010897E-5	2.3599400646021793E-5
3	0.0	0.0	5.899850161505448E-6	1.1799700323010897E-5	2.3599400646021793E-5	2.3599400646021793E-5
4	0.0	0.0	1.1799700323010897E-5	1.1799700323010897E-5	7.669805209957082E-5	2.3599400646021793E-5
5	5.899850161505448E-6	0.0	1.1799700323010897E-5	1.7699550484516343E-5	1.0029745274559262E-4	2.949925080752724E-5
6	5.899850161505448E-6	1.1799700323010897E-5	1.1799700323010897E-5	2.949925080752724E-5	1.1799700323010896E-4	3.5399100969032686E-5
7	1.7699550484516343E-5	1.1799700323010897E-5	1.1799700323010897E-5	2.949925080752724E-5	1.4159640387613074E-4	3.5399100969032686E-5
8	2.3599400646021793E-5	1.7699550484516343E-5	1.7699550484516343E-5	2.949925080752724E-5	1.71095654683658E-4	3.5399100969032686E-5
9	2.3599400646021793E-5	2.3599400646021793E-5	2.3599400646021793E-5	4.1298951130538136E-5	2.064947556526907E-4	4.7198801292043586E-5
10-11	2.949925080752724E-5	2.949925080752724E-5	3.5399100969032686E-5	4.1298951130538136E-5	2.448437817024761E-4	6.489835177655992E-5
12-13	3.5399100969032686E-5	3.5399100969032686E-5	3.5399100969032686E-5	4.1298951130538136E-5	2.949925080752724E-4	7.079820193806537E-5
14-15	3.5399100969032686E-5	3.834902604978541E-5	6.19484266958072E-5	4.1298951130538136E-5	3.42191309367316E-4	7.079820193806537E-5
16-17	4.1298951130538136E-5	4.424887621129086E-5	7.37481270188181E-5	5.3098651453549036E-5	4.0708966114387594E-4	7.964797718032355E-5
18-19	4.7198801292043586E-5	5.3098651453549036E-5	8.5547827341829E-5	7.079820193806537E-5	4.454386871936614E-4	1.0029745274559262E-4
20-21	5.014872637279631E-5	5.3098651453549036E-5	9.73475276648399E-5	7.669805209957082E-5	4.955874135664577E-4	1.1504707814935625E-4
22-23	5.3098651453549036E-5	6.489835177655994E-5	1.091472279878508E-4	8.849775242258172E-5	5.368863646969958E-4	1.2684677847236715E-4
24-25	5.899850161505448E-5	6.489835177655994E-5	1.209469283108617E-4	9.439760258408717E-5	6.31283967281083E-4	1.474962540376362E-4
26-27	7.669805209957082E-5	7.37481270188181E-5	1.2979670355311987E-4	9.439760258408717E-5	6.902824688961375E-4	1.5634602927989437E-4
28-29	1.0619730290709807E-4	7.964797718032355E-5	1.2979670355311987E-4	1.0029745274559262E-4	7.522308955919447E-4	1.946950553296798E-4
30-31	1.0619730290709807E-4	8.259790226107627E-5	1.3864647879537805E-4	1.0324737782634535E-4	8.141793222877519E-4	2.3599400646021794E-4
32-33	1.0619730290709807E-4	8.259790226107627E-5	1.4159640387613074E-4	1.0619730290709807E-4	8.908773743873227E-4	2.536935569447343E-4
34-35	1.091472279878508E-4	8.259790226107627E-5	1.5339610419914164E-4	1.0619730290709807E-4	9.823250518906572E-4	2.56643482025487E-4
36-37	1.1799700323010897E-4	8.259790226107627E-5	1.5339610419914164E-4	1.0619730290709807E-4	0.0010678728792324862	2.831928077522615E-4
38-39	1.2979670355311987E-4	8.259790226107627E-5	1.6519580452215254E-4	1.1209715306860352E-4	0.0011534207065743152	3.09742133479036E-4
40-41	1.5044617911838892E-4	8.5547827341829E-5	1.7404557976441072E-4	1.1209715306860352E-4	0.0012065193580278642	3.333415341250578E-4
42-43	1.71095654683658E-4	8.849775242258172E-5	1.828953550066689E-4	1.1209715306860352E-4	0.0012891172602889404	3.392413842865633E-4
44-45	1.7994542992591617E-4	8.849775242258172E-5	1.9764498041043252E-4	1.2389685339161442E-4	0.0013746650876307696	3.569409347710796E-4
46-47	1.9174513024892707E-4	8.849775242258172E-5	2.0354483057193797E-4	1.4159640387613074E-4	0.0014366135143265768	3.7169056017484324E-4
48-49	2.0059490549118524E-4	9.73475276648399E-5	2.064947556526907E-4	1.4159640387613074E-4	0.001498561941022384	4.011898109823705E-4
50-51	2.064947556526907E-4	1.1504707814935625E-4	2.0944468073344342E-4	1.5339610419914164E-4	0.001563460292798944	4.395388370321559E-4
52-53	2.064947556526907E-4	1.1799700323010896E-4	2.3599400646021792E-4	1.592959543606471E-4	0.0016283586445755038	4.631382376781777E-4
54-55	2.1239460581419614E-4	1.2389685339161442E-4	2.448437817024761E-4	1.6224587944139982E-4	0.0017050566966750746	5.073871138894686E-4
56-57	2.1239460581419614E-4	1.327466286338726E-4	2.5959340710623974E-4	1.6519580452215254E-4	0.0017670051233708817	5.339364396162431E-4
58-59	2.182944559757016E-4	1.3569655371462532E-4	2.625433321869925E-4	1.6519580452215254E-4	0.001817153849743678	5.634356904237704E-4
60-61	2.300941562987125E-4	1.3569655371462532E-4	2.684431823484979E-4	1.6814572960290527E-4	0.0019027016770855072	5.958848663120503E-4
62-63	2.330440813794652E-4	1.4454632895688347E-4	2.743430325100034E-4	1.71095654683658E-4	0.0019911994295080887	6.548833679271048E-4
64-65	2.448437817024761E-4	1.474962540376362E-4	2.772929575907561E-4	1.828953550066689E-4	0.002082647107011423	7.138818695421592E-4
66-67	2.536935569447343E-4	1.5044617911838892E-4	2.861427328330142E-4	1.828953550066689E-4	0.0022094938854837903	7.551808206726974E-4
68-69	2.56643482025487E-4	1.5634602927989437E-4	2.920425829945197E-4	1.828953550066689E-4	0.0023363406639561575	8.141793222877518E-4
70-71	2.5959340710623974E-4	1.6519580452215254E-4	2.949925080752724E-4	1.828953550066689E-4	0.002418938566217234	8.67277973741301E-4
72-73	2.772929575907561E-4	1.6519580452215254E-4	3.038422833175306E-4	1.9174513024892707E-4	0.002557585045012612	9.08576924871839E-4
74-75	2.8909265791376694E-4	1.6519580452215254E-4	3.067922083982833E-4	2.0059490549118524E-4	0.002651982647596699	9.970746772944208E-4
76-77	3.067922083982833E-4	1.7699550484516344E-4	3.09742133479036E-4	2.1239460581419614E-4	0.0027640798006653023	0.0010678728792324862
78-79	3.067922083982833E-4	1.9764498041043252E-4	3.2449175888279964E-4	2.182944559757016E-4	0.0028407778527648735	0.001144570931332057
80-81	3.1269205855978874E-4	2.0059490549118524E-4	3.274416839635524E-4	2.182944559757016E-4	0.0028879766540569167	0.0012242189085123806
82-83	3.2449175888279964E-4	2.0354483057193797E-4	3.451412344480687E-4	2.300941562987125E-4	0.0029233757550259495	0.0012802674850466824
84-85	3.2449175888279964E-4	2.1534453089494887E-4	3.4809115952882144E-4	2.3894393154097064E-4	0.003029573057933048	0.001401214413357544
86-87	3.2449175888279964E-4	2.182944559757016E-4	3.4809115952882144E-4	2.5959340710623974E-4	0.003091521484628855	0.0015221613416684055
88-89	3.303916090443051E-4	2.182944559757016E-4	3.539910096903269E-4	2.654932572677452E-4	0.0031387202859208983	0.001604759243929482
>>END_MODULE
