##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674229
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	56841710
Total Bases	5.6 Gbp
Sequences flagged as poor quality	0
Sequence length	63-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.34048636115979	37.0	36.0	37.0	31.0	37.0
2	34.841410699994775	37.0	35.0	37.0	29.0	37.0
3	35.0360153309955	37.0	35.0	37.0	30.0	37.0
4	35.08623852097342	37.0	35.0	37.0	30.0	37.0
5	34.98129470418817	37.0	35.0	37.0	30.0	37.0
6	34.93183572415397	37.0	35.0	37.0	30.0	37.0
7	35.00119007679396	37.0	35.0	37.0	30.0	37.0
8	34.99753742454265	37.0	35.0	37.0	30.0	37.0
9	35.00233395511852	37.0	35.0	37.0	30.0	37.0
10-11	34.971739089130146	37.0	35.0	37.0	30.0	37.0
12-13	34.943787906802946	37.0	35.0	37.0	30.0	37.0
14-15	34.913976787468215	37.0	35.0	37.0	29.0	37.0
16-17	34.8970091944806	37.0	35.0	37.0	29.0	37.0
18-19	34.87837377693246	37.0	35.0	37.0	29.0	37.0
20-21	34.83683322510882	37.0	35.0	37.0	29.0	37.0
22-23	34.78988722366023	37.0	35.0	37.0	29.0	37.0
24-25	34.753433710914045	37.0	35.0	37.0	29.0	37.0
26-27	34.73090865844817	37.0	35.0	37.0	29.0	37.0
28-29	34.710475696456	37.0	35.0	37.0	29.0	37.0
30-31	34.6360772661484	37.0	35.0	37.0	28.5	37.0
32-33	34.6517007317338	37.0	35.0	37.0	29.0	37.0
34-35	34.581973835762504	37.0	35.0	37.0	28.5	37.0
36-37	34.572388321885455	37.0	35.0	37.0	28.0	37.0
38-39	34.5305438647078	37.0	35.0	37.0	28.0	37.0
40-41	34.50207813065441	37.0	35.0	37.0	28.0	37.0
42-43	34.48626241188029	37.0	35.0	37.0	28.0	37.0
44-45	34.42425460986308	37.0	35.0	37.0	28.0	37.0
46-47	34.346847886877434	37.0	34.5	37.0	27.5	37.0
48-49	34.34333306475122	37.0	35.0	37.0	27.0	37.0
50-51	34.30835654838674	37.0	34.0	37.0	27.0	37.0
52-53	34.21880329603033	37.0	34.0	37.0	27.0	37.0
54-55	34.15832117823338	37.0	34.0	37.0	27.0	37.0
56-57	34.16786556913928	37.0	34.0	37.0	27.0	37.0
58-59	34.10887078344406	37.0	34.0	37.0	26.5	37.0
60-61	34.05593051299829	37.0	34.0	37.0	26.0	37.0
62-63	33.9893016061621	37.0	34.0	37.0	26.0	37.0
64-65	33.9878872394639	37.0	34.0	37.0	26.0	37.0
66-67	33.91875670480404	37.0	34.0	37.0	26.0	37.0
68-69	33.863090590581194	37.0	34.0	37.0	26.0	37.0
70-71	33.83775333479551	37.0	34.0	37.0	25.5	37.0
72-73	33.77195914752248	37.0	34.0	37.0	25.0	37.0
74-75	33.64601772364576	37.0	33.0	37.0	25.0	37.0
76-77	33.646602407587096	37.0	33.0	37.0	25.0	37.0
78-79	33.57806110567449	37.0	33.0	37.0	24.5	37.0
80-81	33.47634538171921	37.0	33.0	37.0	24.0	37.0
82-83	33.423329313479584	37.0	33.0	37.0	24.0	37.0
84-85	33.32274579331748	37.0	33.0	37.0	24.0	37.0
86-87	33.27535391159868	37.0	33.0	37.0	23.0	37.0
88-89	33.15851229560936	37.0	33.0	37.0	23.0	37.0
90-91	33.15065221559898	37.0	33.0	37.0	23.0	37.0
92-93	33.11709143160748	37.0	33.0	37.0	23.0	37.0
94-95	33.01620302847644	36.0	32.0	37.0	22.5	37.0
96-97	32.9915075196392	36.0	32.0	37.0	22.0	37.0
98-99	33.22031224619677	36.5	32.5	37.0	23.5	37.0
100	34.545645171032994	37.0	34.0	37.0	28.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	439.0
20	19658.0
21	127982.0
22	296829.0
23	443879.0
24	551444.0
25	657986.0
26	787254.0
27	955710.0
28	1168790.0
29	1447428.0
30	1805070.0
31	2297777.0
32	3006985.0
33	4143852.0
34	6249868.0
35	1.1347918E7
36	2.0580327E7
37	952514.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	26.807553014157868	29.599015775705283	15.74737755077423	27.846053659362617
2	23.147066393678035	27.08713003894328	30.126016469189516	19.639787098189174
3	17.325628718660255	26.56026446638009	33.43163781366982	22.68246900128983
4	20.981214011144843	27.955556798876223	28.403057231692575	22.660171958286355
5	22.67958295680412	27.56173435929563	27.534744679388663	22.223938004511588
6	22.06708610531657	27.24712113096916	28.331875105711223	22.353917658003045
7	23.064989177362495	27.984781753495575	27.019537567478334	21.930691501663592
8	20.99890880234767	26.79018687186499	28.87599955334342	23.33490477244392
9	21.564841419758977	26.052889207002544	29.43377841287897	22.948490960359504
10-11	22.98730053623431	26.73445664207988	27.34010447510923	22.938138346576576
12-13	22.8820244813022	26.949828063122546	27.138416484023896	23.02973097155136
14-15	22.75518032027484	27.183295721498247	27.187088689077626	22.874435269149288
16-17	23.17493457524082	27.096165186196597	26.834163482126627	22.894736756435954
18-19	22.965156081209546	26.937754685054326	26.986746198833867	23.11034303490226
20-21	22.857516668692526	27.091730369075616	27.139377012634725	22.911375949597133
22-23	23.207828197514235	27.11373592579	26.78485269347921	22.893583183216553
24-25	22.99338580064288	26.940342626588464	27.03358066909363	23.032690903675025
26-27	22.901359618310952	27.0264169080717	27.179129458981166	22.893094014636183
28-29	23.2694687482665	26.987204685331363	26.77009643404924	22.973230132352896
30-31	23.068351461208575	26.84114865643735	26.939450655107883	23.15104922724619
32-33	22.931193495209325	27.011250278036652	27.0957822191689	22.96177400758512
34-35	23.294387030687513	26.971982787084293	26.73351871421203	23.00011146801616
36-37	23.126143835402672	26.80471558670995	26.930928872856313	23.13821170503107
38-39	23.005587944134508	26.942083193434563	27.058515132471534	22.9938137299594
40-41	23.35309353944391	26.91909078608405	26.673145070725962	23.05467060374608
42-43	23.14156397854148	26.793972790625066	26.876341521431478	23.188121709401972
44-45	23.012124423371326	26.926610203050437	27.056793676728784	23.004471696849453
46-47	23.37141356483381	26.866053452707128	26.71493255815619	23.047600424302868
48-49	23.19382222796576	26.730294988327806	26.88807420058204	23.187808583124394
50-51	23.07312479027423	26.8581527336485	27.025337653651953	23.043384822425317
52-53	23.40142945948954	26.84332616877635	26.657353617005615	23.097890754728503
54-55	23.221966641830534	26.694361920361832	26.87327915622526	23.210392281582376
56-57	23.085744051030588	26.834957942651826	27.033401806163738	23.045896200153848
58-59	23.439974938179088	26.79417106783252	26.692141421773375	23.07371257221502
60-61	23.257291504386302	26.65935425836883	26.874373212715565	23.20898102452931
62-63	23.145831364364465	26.774098652462484	27.014607741545387	23.06546224162766
64-65	23.4681044402312	26.77742159692543	26.67250990589194	23.08196405695143
66-67	23.286790567724942	26.63365944829661	26.85947350739461	23.22007647658384
68-69	23.158231725050932	26.78695484555767	27.014774343946456	23.040039085444942
70-71	23.49916772175967	26.738546568278025	26.67747338792471	23.084812322037596
72-73	23.337141979570276	26.600721472649642	26.83577704653209	23.22635950124799
74-75	23.23439993601025	26.716470989217513	26.970823907131457	23.078305167640785
76-77	23.553483163509217	26.704036259894742	26.637122138138103	23.10535843845793
78-79	23.37819326761188	26.568036506282	26.83778244348139	23.21598778262473
80-81	23.284429391838103	26.690412139192887	26.96264057758084	23.062517891388172
82-83	23.611657604787883	26.67348208577131	26.606753077689067	23.108107231751738
84-85	23.425439685637357	26.52592458775543	26.798176268464562	23.25045945814265
86-87	23.340571358556762	26.64932317767663	26.911643734637984	23.098461729128623
88-89	23.69665623898801	26.62362979493847	26.54327991400885	23.136434052064672
90-91	23.509171667714934	26.488317526137994	26.748336892347506	23.254173913799566
92-93	23.392301772386663	26.621647327540426	26.901771584091506	23.08427931598141
94-95	23.746577099642334	26.574973027532415	26.526711302277157	23.15173857054809
96-97	23.56565244788461	26.460551330676886	26.697204467715796	23.276591753722716
98-99	23.386190658250534	26.546297732456996	26.896452723343845	23.17105888594863
100	24.107371290875648	25.78250768903153	26.29622431819841	23.813896701894414
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	31.5
2	24.0
3	17.0
4	20.5
5	35.0
6	44.0
7	67.0
8	136.5
9	235.0
10	437.0
11	800.5
12	1384.5
13	2319.5
14	3927.5
15	6574.5
16	10486.0
17	16367.0
18	25140.5
19	37445.0
20	54806.0
21	78834.0
22	110069.5
23	147814.5
24	192527.0
25	244516.0
26	305337.5
27	373504.0
28	444927.5
29	522135.5
30	607714.5
31	698465.5
32	791611.0
33	893443.0
34	1006885.5
35	1125553.0
36	1250889.5
37	1386655.5
38	1526949.5
39	1670654.0
40	1809093.0
41	1941189.5
42	2066056.5
43	2165951.0
44	2244611.0
45	2298618.0
46	2315664.0
47	2299437.0
48	2254561.0
49	2187751.0
50	2102006.0
51	2002911.5
52	1892621.0
53	1769937.5
54	1643069.0
55	1512566.0
56	1379331.0
57	1253674.5
58	1137066.5
59	1025148.5
60	919444.5
61	819827.0
62	726235.5
63	638989.0
64	556964.0
65	478696.0
66	405069.5
67	337558.0
68	277288.0
69	223920.5
70	176528.5
71	136109.5
72	102769.0
73	75761.5
74	54843.0
75	39406.0
76	27568.0
77	18783.0
78	12639.5
79	8395.0
80	5444.0
81	3516.5
82	2244.5
83	1393.0
84	885.5
85	547.0
86	359.5
87	217.5
88	124.5
89	80.5
90	47.5
91	27.0
92	20.0
93	16.5
94	10.0
95	9.5
96	7.5
97	6.0
98	4.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02465267142737261
2	0.16545596534657384
3	0.045243184978073316
4	0.008215797870964825
5	0.07427996096528412
6	0.14656490805783287
7	0.05400435701177885
8	0.018606055306921627
9	0.016663819578967627
10-11	0.04519392537627739
12-13	0.045134989781271534
14-15	0.045808790762980216
16-17	0.024004379882308257
18-19	0.009387472685110986
20-21	0.024616606361772018
22-23	0.07347333498587569
24-25	0.07471538065973032
26-27	0.059818749295191855
28-29	0.0356885815011547
30-31	0.1038665092939674
32-33	0.025808512798084365
34-35	0.09521177318557095
36-37	0.030372942685925528
38-39	0.09958180357346744
40-41	0.05346953847799442
42-43	0.01024159899482264
44-45	0.04461248614793608
46-47	0.03690423810261866
48-49	0.02219145060906859
50-51	0.03079780675141547
52-53	0.07423949772095174
54-55	0.13777470804449762
56-57	0.025164619431751786
58-59	0.022235432396386385
60-61	0.060096714191040346
62-63	0.021197462215686334
64-65	0.032024899462689445
66-67	0.01563112831272165
68-69	0.03974986708481508
70-71	0.014492882254013692
72-73	0.016878457827588015
74-75	0.10129803796153188
76-77	0.018669403860706753
78-79	0.024173291847210968
80-81	0.01912418633144853
82-83	0.03251755927969441
84-85	0.015475487906473127
86-87	0.036126032802409634
88-89	0.026148512449514726
90-91	0.043234401958824185
92-93	0.02779015319950986
94-95	0.02647795161764275
96-97	0.035125995835871844
98-99	0.03097927664122132
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
63	1.0
64	1.0
65	2.0
66	0.0
67	5.0
68	3.0
69	1.0
70	3.0
71	6.0
72	4.0
73	7.0
74	7.0
75	3.0
76	4.0
77	2.0
78	5.0
79	14.0
80	16.0
81	18.0
82	22.0
83	45.0
84	75.0
85	118.0
86	133.0
87	264.0
88	483.0
89	773.0
90	1398.0
91	2626.0
92	5182.0
93	10113.0
94	21691.0
95	47364.0
96	110839.0
97	278188.0
98	869168.0
99	3692389.0
100	5.1800737E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.71820171375505
#Duplication Level	Percentage of total
1	73.5240648578275
2	14.65419041549081
3	5.546201708247294
4	2.266712005635029
5	1.024578471464242
6	0.5247814149209307
7	0.31333843182391835
8	0.2050555085940047
9	0.14587852535000712
>10	1.3574825085092739
>50	0.3709935645994348
>100	0.060059054616276746
>500	0.0018198878129039465
>1k	0.004843645108381442
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.518542985423908E-6	1.759271492711954E-6	1.759271492711954E-6	1.4074171941695632E-5	1.9351986419831493E-5	5.9815230752206435E-5
2	3.518542985423908E-6	3.518542985423908E-6	5.2778144781358614E-6	2.2870529405255402E-5	2.3046456554526597E-4	8.79635746355977E-5
3	5.2778144781358614E-6	1.4074171941695632E-5	1.5833443434407587E-5	2.6389072390679308E-5	2.973168822683202E-4	8.79635746355977E-5
4	5.2778144781358614E-6	2.8148343883391264E-5	2.2870529405255402E-5	3.1666886868815174E-5	4.450956876561243E-4	8.79635746355977E-5
5	7.037085970847816E-6	3.8703972839662986E-5	2.2870529405255402E-5	3.518542985423908E-5	5.295407193062981E-4	8.79635746355977E-5
6	1.2314900448983678E-5	4.398178731779885E-5	2.6389072390679308E-5	3.518542985423908E-5	6.438933663325752E-4	8.79635746355977E-5
7	1.2314900448983678E-5	5.2778144781358616E-5	2.9907615376103217E-5	4.222251582508689E-5	7.107456830556294E-4	8.972284612830965E-5
8	1.5833443434407587E-5	5.805595925949448E-5	3.1666886868815174E-5	5.4537416274070576E-5	8.673208459069933E-4	9.500066060644551E-5
9	1.759271492711954E-5	6.333377373763035E-5	4.222251582508689E-5	5.805595925949448E-5	9.482473345717432E-4	1.0555628956271723E-4
10-11	2.550943664432333E-5	7.125049545483414E-5	4.6620694556866784E-5	6.245413799127437E-5	0.001103063225930395	1.1347301127992104E-4
12-13	3.0787251122459194E-5	8.268576015746184E-5	5.893559500585046E-5	8.44450316501738E-5	0.0012772311037088785	1.3194536195339655E-4
14-15	3.342615836152713E-5	9.851920359186942E-5	6.245413799127437E-5	1.0203774657729333E-4	0.0014522786172337181	1.381028121778884E-4
16-17	3.782433709330701E-5	1.1875082575805689E-4	7.037085970847815E-5	1.1523228277263298E-4	0.001606214872846014	1.5217698411958402E-4
18-19	3.8703972839662986E-5	1.2754718322161666E-4	7.652830993296999E-5	1.2754718322161666E-4	0.0017407991420384785	1.680104275539916E-4
20-21	4.4861423064154824E-5	1.4162135516331228E-4	8.884321038195368E-5	1.381028121778884E-4	0.0019052910266070461	1.8208459949568725E-4
22-23	4.837996604957873E-5	1.5217698411958402E-4	9.50006606064455E-5	1.495380768805161E-4	0.0020759403614001057	2.0495512890094265E-4
24-25	5.541705202042655E-5	1.5745479859771987E-4	1.0555628956271723E-4	1.5393625561229595E-4	0.002256265689403081	2.2342747957441816E-4
26-27	6.861158821576621E-5	1.680104275539916E-4	1.14352647026277E-4	1.609733415831438E-4	0.002420757573971649	2.436591017406056E-4
28-29	7.30097669475461E-5	1.8296423524204322E-4	1.257879117289047E-4	1.7064933479305953E-4	0.002576453101076657	2.638907239067931E-4
30-31	8.180612441110586E-5	1.9439949994467091E-4	1.3986208367060033E-4	1.7768642076390736E-4	0.002748861707362428	2.867612533120485E-4
32-33	8.796357463559769E-5	2.0407549315458668E-4	1.5217698411958402E-4	1.8824204972017907E-4	0.002982844815893118	3.0787251122459195E-4
34-35	9.763956784551345E-5	2.2078857233535023E-4	1.5921407009043186E-4	2.023162216618747E-4	0.0031974759380039765	3.237059546589995E-4
36-37	1.0907483254814115E-4	2.3398310853068986E-4	1.680104275539916E-4	2.1199221487179046E-4	0.0034094681528757664	3.544932057814587E-4
38-39	1.2051009725076885E-4	2.410201945015377E-4	1.7768642076390736E-4	2.1727002934992634E-4	0.003595071295356878	3.949564501138337E-4
40-41	1.2930645471432862E-4	2.603721809213692E-4	1.873624139738231E-4	2.2782565830619804E-4	0.0038721565554590102	4.239844297435809E-4
42-43	1.381028121778884E-4	2.832427103266246E-4	2.0055695016916276E-4	2.4014055875518173E-4	0.004143964001083008	4.4861423064154824E-4
44-45	1.4689916964144816E-4	2.9027979629747235E-4	2.0671440039365458E-4	2.4893691621874145E-4	0.004416651082453361	4.925960179593471E-4
46-47	1.6009370583678782E-4	3.008354252537441E-4	2.0935330763272253E-4	2.5861290942865724E-4	0.004636560019042354	5.198647260963823E-4
48-49	1.6625115606127964E-4	3.025946967464561E-4	2.1639039360357034E-4	2.6213145241408116E-4	0.00481688534704533	5.568094274433335E-4
50-51	1.7680678501755137E-4	3.1490959719543974E-4	2.2782565830619804E-4	2.656499953995051E-4	0.0050728593492349195	6.157450224491839E-4
52-53	1.8912168546653504E-4	3.298634048834914E-4	2.3574238002340185E-4	2.7884453159484474E-4	0.005373694774488663	6.834769749185941E-4
54-55	1.987976786764508E-4	3.4129866958611905E-4	2.4365910174060562E-4	2.885205248047604E-4	0.005703558179372155	7.485700201489364E-4
56-57	2.0935330763272253E-4	3.5185429854239076E-4	2.5421473069687736E-4	2.9379833928289633E-4	0.006036940127241069	8.303761445600422E-4
58-59	2.1727002934992634E-4	3.588913845132386E-4	2.66529631145861E-4	3.03474332492812E-4	0.0063166642945822705	9.139415404638601E-4
60-61	2.2782565830619804E-4	3.7648409944035814E-4	2.67409266892217E-4	3.131503257027278E-4	0.006638610977748558	0.0010045440223385258
62-63	2.4981655196509747E-4	3.8440082115756194E-4	2.7708526010213275E-4	3.210670474199316E-4	0.007029169249130612	0.0011101003119012428
64-65	2.577332736823012E-4	3.9671572160654563E-4	2.867612533120485E-4	3.325023121225593E-4	0.00741005152730275	0.001223573323181164
66-67	2.6477035965314906E-4	4.055120790701054E-4	3.025946967464561E-4	3.404190338397631E-4	0.007802369070177516	0.0013212128910266773
68-69	2.7268708137035287E-4	4.1518807228002114E-4	3.131503257027278E-4	3.5537284152781473E-4	0.008101445223938547	0.0014593157032045658
70-71	2.7972416734120065E-4	4.2926224422171677E-4	3.2634486189806746E-4	3.738451922012902E-4	0.00848584604509611	0.0015851036149334705
72-73	2.9379833928289633E-4	4.450956876561244E-4	3.4217830533247507E-4	3.896786356356978E-4	0.008999553320967999	0.0017029748049451716
74-75	3.03474332492812E-4	4.618087668368879E-4	3.5185429854239076E-4	3.9847499309925757E-4	0.009411222850262597	0.0018243645379422964
76-77	3.1139105421001587E-4	4.7940148176400744E-4	3.624099274986625E-4	4.063917148164613E-4	0.009843124001723384	0.00198533787952544
78-79	3.175485044345077E-4	4.93475653705703E-4	3.703266492158663E-4	4.143084365336652E-4	0.010187941214294925	0.002145431585362228
80-81	3.228263189126436E-4	5.040312826619748E-4	3.7560446369400217E-4	4.2134552250451296E-4	0.010505489718729433	0.0023134420129162195
82-83	3.272244976444235E-4	5.172258188573145E-4	3.8703972839662984E-4	4.301418799680728E-4	0.01081864004443216	0.002482332076216567
84-85	3.333819478689153E-4	5.216239975890944E-4	4.063917148164613E-4	4.3981787317798847E-4	0.011125632919910398	0.0026776112119075938
86-87	3.43057941078831E-4	5.295407193062981E-4	4.160677080263771E-4	4.4949386638790426E-4	0.011345541856499391	0.002861455082895993
88-89	3.544932057814587E-4	5.453741627407057E-4	4.2838260847536075E-4	4.5389204511968415E-4	0.01159975658719627	0.0030452989538843926
>>END_MODULE
