##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674235
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1398529
Total Bases	139.2 Mbp
Sequences flagged as poor quality	0
Sequence length	82-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.11283856108812	37.0	35.0	37.0	31.0	37.0
2	34.73903794629929	37.0	35.0	37.0	29.0	37.0
3	34.61062587904863	37.0	35.0	37.0	29.0	37.0
4	34.524280869399206	37.0	35.0	37.0	28.0	37.0
5	34.45860757982137	37.0	35.0	37.0	28.0	37.0
6	34.39800390267202	37.0	35.0	37.0	28.0	37.0
7	34.43700416652068	37.0	35.0	37.0	28.0	37.0
8	34.39189319635131	37.0	35.0	37.0	28.0	37.0
9	34.393261062158885	37.0	35.0	37.0	28.0	37.0
10-11	34.26167959334415	36.5	34.0	37.0	27.0	37.0
12-13	34.13838039826132	36.0	34.0	37.0	27.0	37.0
14-15	34.02739700070574	36.5	34.0	37.0	26.5	37.0
16-17	34.0077878256368	36.0	34.0	37.0	26.5	37.0
18-19	33.70756809476242	36.0	33.5	37.0	25.5	37.0
20-21	33.93947032918159	36.0	34.0	37.0	26.5	37.0
22-23	33.692276313183356	36.0	33.0	37.0	25.5	37.0
24-25	33.61697326262094	36.0	33.0	37.0	25.0	37.0
26-27	33.55876424443112	36.0	33.0	37.0	25.0	37.0
28-29	33.42095623329942	36.0	33.0	37.0	24.0	37.0
30-31	33.026743456875046	36.0	32.5	37.0	23.0	37.0
32-33	33.15320597570734	36.0	32.5	37.0	23.5	37.0
34-35	33.042441021959505	36.0	32.0	37.0	23.0	37.0
36-37	32.782590850815396	36.0	32.0	37.0	22.0	37.0
38-39	32.87690101528106	36.0	32.0	37.0	22.5	37.0
40-41	32.50464237781269	36.0	31.5	37.0	21.5	37.0
42-43	32.51132761637406	36.0	31.0	37.0	21.0	37.0
44-45	32.43546969708887	36.0	31.0	37.0	21.0	37.0
46-47	32.36066359725111	36.0	31.0	37.0	20.5	37.0
48-49	32.1474341969312	36.0	31.0	37.0	20.0	37.0
50-51	32.058247272670066	36.0	30.5	37.0	19.5	37.0
52-53	31.73178925857097	36.0	30.0	37.0	18.5	37.0
54-55	31.665573970936606	36.0	29.5	37.0	18.5	37.0
56-57	31.41460491702353	36.0	29.0	37.0	17.5	37.0
58-59	31.12392449495148	35.5	28.5	37.0	16.5	37.0
60-61	30.902968762177974	35.0	28.0	37.0	16.0	37.0
62-63	30.870818552922394	35.0	28.0	37.0	16.0	37.0
64-65	30.44137340019406	35.0	27.0	37.0	14.5	37.0
66-67	30.433777919514004	35.0	27.0	37.0	15.0	37.0
68-69	30.254955027747013	35.0	26.0	37.0	14.0	37.0
70-71	29.833792506269084	35.0	25.5	37.0	13.5	37.0
72-73	29.605618474840348	34.0	25.0	37.0	13.0	37.0
74-75	29.553156924168178	34.0	24.5	37.0	13.0	37.0
76-77	29.484025715591166	34.0	24.0	37.0	13.0	37.0
78-79	29.07276252405206	34.0	23.5	37.0	11.5	37.0
80-81	29.021210857980066	34.0	23.0	37.0	12.0	37.0
82-83	28.69610878441318	33.0	22.5	37.0	11.0	37.0
84-85	28.435896528349808	33.0	22.0	37.0	11.0	37.0
86-87	28.035338516870283	32.5	21.0	37.0	10.5	37.0
88-89	27.918758878771197	32.5	21.0	37.0	10.0	37.0
90-91	27.622989257356213	32.0	20.0	36.5	10.0	37.0
92-93	27.316177831192313	31.5	19.5	36.0	10.0	37.0
94-95	27.2919695522707	31.5	19.5	36.0	10.0	37.0
96-97	27.127268019562045	31.0	19.0	36.0	10.0	37.0
98-99	27.601265837373838	31.5	21.0	36.0	10.5	37.0
100	32.27999040832287	34.0	29.0	37.0	23.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	119.0
21	3188.0
22	17429.0
23	35381.0
24	44826.0
25	50568.0
26	55929.0
27	63489.0
28	71666.0
29	82811.0
30	97739.0
31	117800.0
32	145147.0
33	176878.0
34	202335.0
35	185296.0
36	47922.0
37	5.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	27.174045179236717	21.11567972655049	25.533848673154896	26.1764264210579
2	27.44339531574563	29.97229203834067	22.90136323171364	19.682949414200063
3	16.93908735791138	27.22030788460012	31.675403339694864	24.16520141779364
4	19.367723464106597	28.918228218479484	30.28590591933369	21.428142398080233
5	21.41849408174225	26.251851131487363	29.41137772807263	22.91827705869776
6	21.63473674349823	28.519615253424025	27.681459239559874	22.164188763517867
7	21.748973194366584	28.47354902925395	28.453885272346607	21.323592504032856
8	20.79986900287736	26.952729488081552	29.352722623603505	22.894678885437582
9	21.16648599793494	26.287371970700203	29.763791125971395	22.78235090539346
10-11	22.328976211681812	26.58150501434278	28.184292695030923	22.90522607894448
12-13	22.433169401916118	27.116478075215845	27.715541685230228	22.734810837637813
14-15	22.60451666563717	27.11184500662948	27.42252558060026	22.86111274713309
16-17	22.497371280330384	27.101715451866276	27.656740873846843	22.744172393956504
18-19	22.425264800148256	27.26145001156458	27.573190861539654	22.74009432674751
20-21	22.565773220350255	27.162560417476318	27.49377278559827	22.777893576575163
22-23	22.45606106490309	27.259462553626467	27.69054804742262	22.593928334047817
24-25	22.46171163037497	27.325272272652022	27.721376732957015	22.491639364015995
26-27	22.62148616957489	27.17186997759258	27.543074490112236	22.6635693627203
28-29	22.653041157214805	27.05513432723749	27.606674258907326	22.68515025664038
30-31	22.56473928071418	27.199245844546688	27.559869531681393	22.676145343057744
32-33	22.66846969317204	27.10682538734737	27.455011640176075	22.769693279304516
34-35	22.570154960735103	27.170099253675577	27.663989891206757	22.59575589438256
36-37	22.577736279324494	27.283337672714868	27.67251735004444	22.466408697916197
38-39	22.74070554111616	27.029330861890617	27.45178594989107	22.77817764710215
40-41	22.74529306312292	26.96363269848331	27.549194798213563	22.74187944018021
42-43	22.650132392195566	27.149285833051746	27.53818455525698	22.662397219495713
44-45	22.7586137848448	27.039770067841857	27.441643594427763	22.75997255288558
46-47	22.63241623718943	27.075105386257754	27.58307626678948	22.70940210976333
48-49	22.692261069777206	27.16955332562636	27.521516297820103	22.616669306776334
50-51	22.936295557771924	26.90140457243273	27.35567255657072	22.806627313224624
52-53	22.89114704807433	26.841328318274567	27.406845569518858	22.860679064132245
54-55	22.722389934138207	27.108968403647182	27.472749495612668	22.69589216660194
56-57	22.998846597462514	26.871024561215272	27.354917160662716	22.775211680659496
58-59	22.91385801282338	26.876049612347646	27.376286585529435	22.833805789299543
60-61	23.01325332406187	26.94300054798911	27.30702699454443	22.736719133404588
62-63	23.12856239484497	26.770467317530986	27.17552877957217	22.925441508051872
64-65	23.046837247185824	26.761574939846994	27.314116707427356	22.877471105539826
66-67	23.021766736420382	26.95963227271914	27.322136967680777	22.696464023179697
68-69	23.19312298203479	26.765024249565556	27.168062832094563	22.873789936305094
70-71	23.288890097839065	26.72664332120247	27.13155016363777	22.852916417320692
72-73	23.32281883877755	26.78483694183868	27.031674094802217	22.860670124581553
74-75	23.46114307776036	26.683073602568815	26.875541416361305	22.980241903309523
76-77	23.337867387154294	26.70853451557415	27.123146428234108	22.830451669037444
78-79	23.32497641191535	26.844875769420856	27.04396818978299	22.786179628880802
80-81	23.576389721399053	26.624349106274742	26.872915270286178	22.926345902040023
82-83	23.643214677406885	26.529349945735746	26.89842562074584	22.92900975611153
84-85	23.588052948461876	26.772513476944226	26.77380210906123	22.865631465532672
86-87	23.853928194528446	26.532658529921214	26.56227338992028	23.05113988563006
88-89	23.802822145543775	26.601517832273736	26.652105081136508	22.943554941045985
90-91	23.914981275151945	26.726448180858718	26.47439600412423	22.884174539865107
92-93	24.07609416267394	26.566266139638557	26.27785801060029	23.079781687087213
94-95	24.051761276332982	26.515789020204778	26.38360354409294	23.0488461593693
96-97	24.078155255887683	26.69039752079983	26.36397143164677	22.867475791665715
98-99	24.07292103746531	26.668398580551173	26.138710861609145	23.119969520374372
100	24.004863497698594	26.15960789542286	26.384574513600477	23.450954093278064
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	0.0
7	0.5
8	1.5
9	1.0
10	0.5
11	3.0
12	7.0
13	17.0
14	28.0
15	45.0
16	89.0
17	161.0
18	263.0
19	444.0
20	744.0
21	1140.0
22	1706.0
23	2443.0
24	3432.5
25	4736.5
26	6288.5
27	7978.5
28	9763.5
29	11951.5
30	14421.0
31	16926.5
32	19719.5
33	22703.5
34	25929.0
35	29598.0
36	33843.0
37	38075.5
38	42258.5
39	46606.0
40	50637.0
41	54257.0
42	57316.5
43	59821.0
44	61476.0
45	62054.5
46	62059.5
47	61183.0
48	58986.0
49	56034.0
50	52797.0
51	49275.0
52	45402.0
53	41167.0
54	37303.0
55	33726.0
56	29954.5
57	26545.0
58	23448.0
59	20522.0
60	17957.5
61	15848.5
62	13987.5
63	12156.0
64	10449.5
65	8959.0
66	7612.5
67	6393.5
68	5363.5
69	4444.5
70	3625.5
71	2874.0
72	2190.0
73	1728.5
74	1350.0
75	1036.0
76	733.0
77	506.0
78	374.0
79	261.0
80	178.0
81	127.0
82	90.5
83	56.5
84	30.0
85	18.5
86	16.0
87	12.5
88	7.0
89	2.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007078866437521138
2	0.0010010518194474337
3	0.0012870666250038433
4	0.001930599937505765
5	0.004290222083346145
6	0.007293377541688446
7	0.001215562923614741
8	0.0017875925347275601
9	0.0015015777291711506
10-11	0.006471084975713769
12-13	0.012870666250038434
14-15	0.44782768179994836
16-17	0.002466877697924033
18-19	0.45383399271663294
20-21	0.0021808628923676234
22-23	0.005899055364600949
24-25	0.011476344072950936
26-27	0.0077581516007176115
28-29	0.012262884788231062
30-31	0.48433032135908516
32-33	0.009974766343779785
34-35	0.010189277447947093
36-37	0.4462903522200827
38-39	0.011118825566005424
40-41	0.503779328136921
42-43	0.015838069857686186
44-45	0.01455100323268234
46-47	0.01576656615629708
48-49	0.0163385957674099
50-51	0.02470452882993488
52-53	0.024311258472294817
54-55	0.021308103013952518
56-57	0.03514406923274383
58-59	0.04944480951056432
60-61	0.04940905765986976
62-63	0.04240169492373773
64-65	0.491194676692439
66-67	0.053913790847383215
68-69	0.05520085747238706
70-71	0.1101872038406068
72-73	0.08948688228846166
74-75	0.08240801585094053
76-77	0.068429042229371
78-79	0.5338823864217331
80-81	0.09406311917736422
82-83	0.11873193860546996
84-85	0.12141337177375068
86-87	0.16167096704765288
88-89	0.1353602814464172
90-91	0.15950714045638853
92-93	0.18239447930293043
94-95	0.1767123194396811
96-97	0.1876674148524217
98-99	0.5101643056269771
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
82	1.0
83	0.0
84	0.0
85	4.0
86	9.0
87	10.0
88	30.0
89	45.0
90	120.0
91	250.0
92	531.0
93	1118.0
94	2411.0
95	5428.0
96	12679.0
97	31577.0
98	84765.0
99	254513.0
100	1005038.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.64755078654848
#Duplication Level	Percentage of total
1	97.38974873673504
2	2.3611966346401996
3	0.19655436545862498
4	0.03450146654441422
5	0.00922169969609142
6	0.0035800470169631508
7	0.001234854882331645
8	0.0012772238980226752
9	0.0013204489707542562
>10	0.0013645221575486926
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	7.15037013891024E-5	0.0	0.0	0.0	1.430074027782048E-4
2	0.0	7.15037013891024E-5	0.0	0.0	0.0	2.1451110416730723E-4
3	0.0	7.15037013891024E-5	0.0	0.0	0.0	2.1451110416730723E-4
4	0.0	7.15037013891024E-5	0.0	0.0	0.0	2.1451110416730723E-4
5	0.0	7.15037013891024E-5	0.0	0.0	0.0	2.1451110416730723E-4
6	0.0	7.15037013891024E-5	0.0	0.0	7.15037013891024E-5	2.1451110416730723E-4
7	0.0	7.15037013891024E-5	0.0	0.0	1.430074027782048E-4	2.1451110416730723E-4
8	0.0	7.15037013891024E-5	0.0	0.0	1.430074027782048E-4	2.1451110416730723E-4
9	0.0	1.430074027782048E-4	0.0	0.0	1.430074027782048E-4	2.1451110416730723E-4
10-11	0.0	1.430074027782048E-4	0.0	0.0	1.7875925347275604E-4	2.1451110416730723E-4
12-13	0.0	1.7875925347275604E-4	0.0	0.0	2.860148055564096E-4	2.1451110416730723E-4
14-15	0.0	2.5026295486185843E-4	0.0	0.0	2.860148055564096E-4	2.5026295486185843E-4
16-17	0.0	2.860148055564096E-4	0.0	0.0	2.860148055564096E-4	2.860148055564096E-4
18-19	0.0	2.860148055564096E-4	1.0725555208365362E-4	0.0	2.860148055564096E-4	3.932703576400632E-4
20-21	0.0	2.860148055564096E-4	1.430074027782048E-4	0.0	3.57518506945512E-4	4.2902220833461447E-4
22-23	0.0	3.217666562509608E-4	1.430074027782048E-4	7.15037013891024E-5	3.57518506945512E-4	4.2902220833461447E-4
24-25	0.0	3.57518506945512E-4	1.430074027782048E-4	7.15037013891024E-5	4.6477405902916566E-4	4.2902220833461447E-4
26-27	0.0	3.57518506945512E-4	1.430074027782048E-4	7.15037013891024E-5	5.005259097237169E-4	4.2902220833461447E-4
28-29	0.0	3.57518506945512E-4	1.430074027782048E-4	7.15037013891024E-5	6.077814618073705E-4	4.2902220833461447E-4
30-31	0.0	3.57518506945512E-4	1.430074027782048E-4	7.15037013891024E-5	6.792851631964728E-4	5.36277760418268E-4
32-33	0.0	3.57518506945512E-4	1.430074027782048E-4	7.15037013891024E-5	8.580444166692289E-4	6.077814618073705E-4
34-35	0.0	3.57518506945512E-4	1.430074027782048E-4	7.15037013891024E-5	9.295481180583313E-4	7.15037013891024E-4
36-37	0.0	3.57518506945512E-4	1.430074027782048E-4	7.15037013891024E-5	0.0010368036701419849	8.222925659746777E-4
38-39	0.0	3.57518506945512E-4	1.430074027782048E-4	1.0725555208365362E-4	0.001072555520836536	8.580444166692289E-4
40-41	0.0	4.2902220833461447E-4	1.430074027782048E-4	1.430074027782048E-4	0.0011083073715310874	9.295481180583313E-4
42-43	0.0	4.2902220833461447E-4	2.1451110416730723E-4	1.430074027782048E-4	0.001215562923614741	0.0010010518194474337
44-45	3.57518506945512E-5	5.005259097237169E-4	2.1451110416730723E-4	1.430074027782048E-4	0.0012870666250038433	0.001072555520836536
46-47	7.15037013891024E-5	5.005259097237169E-4	2.1451110416730723E-4	1.430074027782048E-4	0.001394322177087497	0.0011798110729201896
48-49	1.0725555208365362E-4	5.36277760418268E-4	2.1451110416730723E-4	1.430074027782048E-4	0.0016445851319493554	0.001215562923614741
50-51	1.430074027782048E-4	5.720296111128193E-4	2.1451110416730723E-4	1.430074027782048E-4	0.0018948480868112138	0.0015015777291711506
52-53	1.430074027782048E-4	6.435333125019216E-4	2.1451110416730723E-4	1.430074027782048E-4	0.002145111041673072	0.0016088332812548042
54-55	1.430074027782048E-4	6.435333125019216E-4	2.1451110416730723E-4	1.430074027782048E-4	0.0023238702951458284	0.0016803369826439065
56-57	1.430074027782048E-4	6.435333125019216E-4	2.1451110416730723E-4	1.430074027782048E-4	0.0025026295486185843	0.0018233443854221115
58-59	1.430074027782048E-4	6.435333125019216E-4	2.1451110416730723E-4	2.1451110416730723E-4	0.0028243962048695452	0.0018590962361166626
60-61	1.430074027782048E-4	6.435333125019216E-4	2.1451110416730723E-4	2.1451110416730723E-4	0.0032534184132041594	0.001966351788200316
62-63	1.430074027782048E-4	6.435333125019216E-4	2.1451110416730723E-4	2.1451110416730723E-4	0.0034679295173714667	0.0021808628923676234
64-65	1.430074027782048E-4	6.435333125019216E-4	2.5026295486185843E-4	2.1451110416730723E-4	0.003968455427095184	0.0023953739965349307
66-67	1.430074027782048E-4	6.792851631964728E-4	2.860148055564096E-4	2.1451110416730723E-4	0.0044332294861243494	0.0026813888020913402
68-69	1.430074027782048E-4	7.15037013891024E-4	2.860148055564096E-4	2.1451110416730723E-4	0.005005259097237169	0.00296740360764775
70-71	1.7875925347275604E-4	7.15037013891024E-4	2.860148055564096E-4	2.1451110416730723E-4	0.005434281305571782	0.0035394332187605694
72-73	2.1451110416730723E-4	7.15037013891024E-4	2.860148055564096E-4	2.1451110416730723E-4	0.006149318319462807	0.00418296653126249
74-75	2.1451110416730723E-4	7.15037013891024E-4	2.860148055564096E-4	2.1451110416730723E-4	0.006971610885437485	0.004325973934040696
76-77	2.1451110416730723E-4	7.15037013891024E-4	2.860148055564096E-4	2.1451110416730723E-4	0.007615144197939407	0.004719244291680759
78-79	2.1451110416730723E-4	7.15037013891024E-4	2.860148055564096E-4	2.1451110416730723E-4	0.00833018121183043	0.005005259097237169
80-81	2.1451110416730723E-4	7.15037013891024E-4	2.860148055564096E-4	2.1451110416730723E-4	0.009295481180583313	0.005255522052099027
82-83	2.5026295486185843E-4	7.15037013891024E-4	2.860148055564096E-4	2.1451110416730723E-4	0.01036803670141985	0.005613040559044539
84-85	2.860148055564096E-4	7.15037013891024E-4	2.860148055564096E-4	2.1451110416730723E-4	0.011476344072950936	0.00622082202085191
86-87	2.860148055564096E-4	7.507888645855753E-4	2.860148055564096E-4	2.1451110416730723E-4	0.01276341069795478	0.0065783405277974214
88-89	2.860148055564096E-4	7.865407152801264E-4	2.860148055564096E-4	2.1451110416730723E-4	0.014372243979209585	0.0071861219896047915
>>END_MODULE
