##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674244
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27913242
Total Bases	2.7 Gbp
Sequences flagged as poor quality	0
Sequence length	61-100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.57882660136719	37.0	36.0	37.0	32.0	38.0
2	35.26232474178384	37.0	36.0	37.0	31.0	37.0
3	35.350715083543506	37.0	36.0	37.0	31.0	38.0
4	35.49434999345472	37.0	36.0	37.0	32.0	38.0
5	35.49632934074802	37.0	36.0	37.0	32.0	38.0
6	35.16123275827294	37.0	36.0	37.0	31.0	38.0
7	35.46897078454735	37.0	36.0	37.0	32.0	38.0
8	35.46144081006427	37.0	36.0	37.0	32.0	38.0
9	35.477339823156335	37.0	36.0	37.0	32.0	38.0
10-11	35.46751470144528	37.0	36.0	37.0	32.0	38.0
12-13	35.377496691355304	37.0	36.0	37.0	31.5	38.0
14-15	35.44925963813161	37.0	36.0	37.0	31.5	38.0
16-17	35.405868028514924	37.0	36.0	37.0	31.0	38.0
18-19	35.38019005459846	37.0	36.0	37.0	31.0	38.0
20-21	35.37817332361465	37.0	36.0	37.0	31.0	38.0
22-23	35.325642610772334	37.0	36.0	37.0	31.0	38.0
24-25	35.307486908901524	37.0	36.0	37.0	31.0	38.0
26-27	35.290674189691046	37.0	36.0	37.0	31.0	38.0
28-29	35.28320409718083	37.0	36.0	37.0	31.0	38.0
30-31	35.2419502184662	37.0	36.0	37.0	31.0	38.0
32-33	35.19686344567213	37.0	36.0	37.0	31.0	38.0
34-35	35.18158945850862	37.0	36.0	37.0	31.0	38.0
36-37	35.14223190197684	37.0	36.0	37.0	31.0	38.0
38-39	35.107496130331256	37.0	36.0	37.0	30.5	38.0
40-41	35.062145970002334	37.0	36.0	37.0	30.0	38.0
42-43	35.046125509175894	37.0	36.0	37.0	30.0	38.0
44-45	34.86779659990767	37.0	35.5	37.0	29.5	38.0
46-47	34.9349808954474	37.0	35.5	37.0	30.0	38.0
48-49	34.962833195800044	37.0	35.5	37.0	30.0	38.0
50-51	34.87537789411921	37.0	35.0	37.0	30.0	38.0
52-53	34.88176224746663	37.0	35.0	37.0	30.0	38.0
54-55	34.75736091493779	37.0	35.0	37.0	29.0	38.0
56-57	34.7563734445465	37.0	35.0	37.0	29.0	38.0
58-59	34.680474897899714	37.0	35.0	37.0	29.0	38.0
60-61	34.60171143860681	37.0	35.0	37.0	28.5	38.0
62-63	34.584678710725186	37.0	35.0	37.0	28.5	38.0
64-65	34.56049330544091	37.0	35.0	37.0	28.5	38.0
66-67	34.53058638991489	37.0	35.0	37.0	28.0	38.0
68-69	34.481850523927186	37.0	35.0	37.0	28.0	38.0
70-71	34.4023484145575	37.0	35.0	37.0	28.0	38.0
72-73	34.27999975951557	37.0	35.0	37.0	27.0	38.0
74-75	34.29165509869445	37.0	35.0	37.0	27.0	38.0
76-77	34.23915472922103	37.0	35.0	37.0	27.0	38.0
78-79	34.17490489642725	37.0	35.0	37.0	27.0	38.0
80-81	34.09733619708914	37.0	34.0	37.0	26.5	38.0
82-83	34.03156445047977	37.0	34.0	37.0	26.0	38.0
84-85	33.950055033788374	37.0	34.0	37.0	26.0	38.0
86-87	33.88226180475512	37.0	34.0	37.0	25.5	38.0
88-89	33.809832583785564	37.0	34.0	37.0	25.0	38.0
90-91	33.73515563393943	37.0	34.0	37.0	25.0	38.0
92-93	33.6172702691669	37.0	34.0	37.0	24.5	38.0
94-95	33.6107707249775	37.0	34.0	37.0	24.5	38.0
96-97	33.54540870718262	37.0	33.0	37.0	24.0	38.0
98-99	33.602518961185496	37.0	33.5	37.0	24.5	38.0
100	34.72691202266441	37.0	34.0	37.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	601.0
21	11798.0
22	56073.0
23	110174.0
24	150541.0
25	188832.0
26	241523.0
27	311675.0
28	405223.0
29	531504.0
30	700519.0
31	938198.0
32	1290224.0
33	1867244.0
34	2926377.0
35	5751698.0
36	1.2063261E7
37	367771.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.80245029564028	22.407644365577536	17.46579744842193	27.32410789036025
2	29.736167010108094	28.04672982891878	22.6270082684752	19.590094892497923
3	17.78415125018048	25.252641694272015	31.310641427064347	25.65256562848316
4	20.44039748345934	27.559273302068473	28.83314762444372	23.16718159002847
5	22.517480217498157	27.001900907259824	27.719439029394756	22.761179845847266
6	21.54665905288512	28.598902567768032	27.36489560381321	22.489542775533643
7	23.418174130971313	27.94638784553746	26.728234775541825	21.907203247949404
8	22.022639050363544	26.745163810424703	27.623785071862024	23.608412067349732
9	21.46567601893976	25.199690559380155	29.465785550367467	23.868847871312614
10-11	23.507762863551516	25.62503568521911	26.8555606056973	24.011640845532078
12-13	23.554520822564502	26.31636451241978	26.385194674645152	23.743919990370564
14-15	23.54937347894104	26.598355037156463	26.254791635984375	23.597479847918123
16-17	23.781353700469015	26.38005553924109	26.243470358675854	23.59512040161404
18-19	23.486740168979633	26.4382573977148	26.492150278084896	23.58285215522067
20-21	23.53969433543753	26.479902717191557	26.43551367268944	23.54488927468147
22-23	23.708797411069956	26.50160046538922	26.29945499699347	23.490147126547352
24-25	23.422427166997696	26.625787802318694	26.63163057507755	23.320154455606055
26-27	23.513473952137378	26.5824132602803	26.554069951758656	23.35004283582366
28-29	23.75259508636713	26.39455439334888	26.332745143916668	23.52010537636732
30-31	23.516763902201706	26.47997866795559	26.526790335761103	23.4764670940816
32-33	23.539716681103894	26.535333839630653	26.43311699055134	23.491832488714113
34-35	23.73917072015037	26.467632603487978	26.288340047965075	23.504856628396578
36-37	23.53197470226716	26.55974672087915	26.52495815362959	23.383320423224102
38-39	23.604846948378714	26.531047017185283	26.39993969704718	23.46416633738882
40-41	23.835739675741802	26.367680488584767	26.20482499392296	23.591754841750472
42-43	23.565063870156	26.50838609272852	26.425696667631577	23.50085336948391
44-45	23.567615175616154	26.56503257980372	26.37656992323362	23.4907823213465
46-47	23.769080922806694	26.448163695927086	26.263853297904827	23.518902083361393
48-49	23.589520891570277	26.522789151069855	26.483785692132628	23.403904265227247
50-51	23.656008460910137	26.5006479125381	26.35513506842876	23.488208558123002
52-53	23.83086276708042	26.389650414172127	26.199219384860967	23.580267433886483
54-55	23.58725267733464	26.518430275313598	26.44752132553582	23.44679572181594
56-57	23.610113770393703	26.542973662303382	26.398614808640396	23.44829775866252
58-59	23.838256014533115	26.389420862824387	26.260077028371875	23.51224609427062
60-61	23.645437996406756	26.469098842946572	26.447962516383118	23.437500644263558
62-63	23.66723774743078	26.48388493859813	26.354117452000054	23.494759861971044
64-65	23.874335879733163	26.38167242898434	26.1889409728649	23.555050718417593
66-67	23.627506597067043	26.50389912511788	26.457010095355894	23.411584182459176
68-69	23.66187278284381	26.53512198248484	26.389241134769815	23.413764099901538
70-71	23.90356479796606	26.365746610643235	26.218009288490585	23.512679302900118
72-73	23.69663728024879	26.46869972360562	26.400946923354496	23.433716072791096
74-75	23.727605927013702	26.469807615574663	26.326212470655868	23.476373986755764
76-77	23.920189412389156	26.37950514096566	26.179383936813334	23.52092150983185
78-79	23.702003547996615	26.47628145413819	26.420994074081612	23.400720923783584
80-81	23.75958701943356	26.470447346529134	26.340652860496423	23.429312773540886
82-83	23.989313366774354	26.322995617460432	26.160476322359493	23.52721469340572
84-85	23.77974985975743	26.427936373909944	26.342669965887524	23.449643800445102
86-87	23.815263187568565	26.443904218607685	26.264236220116103	23.476596373707643
88-89	24.01103445419338	26.339647326423833	26.136793394046492	23.512524825336296
90-91	23.830846679645447	26.413607995746563	26.338860273118513	23.41668505148948
92-93	23.884254888365525	26.402327681621834	26.23604934312742	23.477368086885217
94-95	24.083470447384723	26.27808827162935	26.087030960674905	23.55141032031102
96-97	23.872107794184174	26.399490853182307	26.26710384795883	23.46129750467469
98-99	23.87761021729827	26.42889045751236	26.195579866413997	23.497919458775375
100	24.2803436631393	25.76682780943449	25.988233886290736	23.964594641135477
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.5
4	2.0
5	5.0
6	8.5
7	11.0
8	18.0
9	38.5
10	76.5
11	133.0
12	240.0
13	447.5
14	800.0
15	1380.5
16	2376.5
17	3933.0
18	6311.5
19	10120.5
20	15625.5
21	23090.0
22	32913.5
23	46209.0
24	63993.5
25	85862.0
26	112401.0
27	144613.5
28	181557.0
29	222687.5
30	269666.5
31	321783.5
32	377559.0
33	438573.0
34	504649.0
35	572717.5
36	642149.5
37	713271.0
38	779922.5
39	843194.5
40	900787.5
41	948818.0
42	989117.5
43	1016749.0
44	1031667.5
45	1036176.0
46	1032060.0
47	1017608.5
48	992469.5
49	962587.5
50	926799.0
51	884495.0
52	841386.5
53	799066.0
54	759847.5
55	724107.0
56	689754.5
57	655928.0
58	623304.0
59	589895.0
60	555812.5
61	520772.0
62	481030.0
63	436256.0
64	389293.0
65	342307.0
66	293576.5
67	246260.0
68	203154.0
69	162904.0
70	126883.0
71	97105.0
72	72787.5
73	52664.5
74	36651.0
75	25204.0
76	17127.0
77	11342.5
78	7444.5
79	4751.0
80	2966.0
81	1825.5
82	1140.5
83	708.0
84	425.5
85	253.5
86	169.5
87	111.5
88	71.0
89	45.5
90	25.5
91	12.5
92	8.0
93	6.0
94	4.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14576235895493614
2	0.05803697040995811
3	0.303440209489102
4	0.05923353510853379
5	0.006566775726015631
6	0.6988940947812512
7	0.04764763620076808
8	0.053691362687286553
9	0.04504672011943292
10-11	0.04669826600579037
12-13	0.20418624250096068
14-15	0.07109887128123633
16-17	0.024529576320801435
18-19	0.0315764109378624
20-21	0.03971949944044479
22-23	0.04404719451792809
24-25	0.024973809921470247
26-27	0.06997753969245135
28-29	0.022983715041054707
30-31	0.027965221667909445
32-33	0.026413986594606247
34-35	0.024706911508165192
36-37	0.047545534123195
38-39	0.02678836087904085
40-41	0.054441902520674594
42-43	0.0012968755116299282
44-45	0.3032682480952947
46-47	0.1224705464166434
48-49	0.001192982169538028
50-51	0.048876443660682625
52-53	0.0018324636027588625
54-55	0.10097895471977063
56-57	0.02788461476456228
58-59	0.07355827746558426
60-61	0.08193423035561401
62-63	0.16236022471127995
64-65	0.028975500046805748
66-67	0.00568189308927035
68-69	0.0038225601265063198
70-71	0.010296196015927435
72-73	0.00785111998026741
74-75	0.0062318189726627
76-77	0.005689070174859734
78-79	0.03205837077521877
80-81	0.007417659457063512
82-83	0.03445339035225538
84-85	0.03747177794208986
86-87	0.014998449640730438
88-89	0.011252969623215755
90-91	0.038691266088429974
92-93	0.06427329426797837
94-95	0.03869630667918898
96-97	0.014962951706445123
98-99	0.05305864305343432
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
61	1.0
62	1.0
63	2.0
64	2.0
65	3.0
66	2.0
67	1.0
68	0.0
69	6.0
70	5.0
71	3.0
72	2.0
73	4.0
74	23.0
75	5.0
76	18.0
77	10.0
78	15.0
79	12.0
80	19.0
81	21.0
82	28.0
83	37.0
84	30.0
85	62.0
86	90.0
87	119.0
88	178.0
89	303.0
90	512.0
91	946.0
92	2113.0
93	3896.0
94	8355.0
95	18711.0
96	44481.0
97	117176.0
98	349651.0
99	1669724.0
100	2.5696675E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.85714274293701
#Duplication Level	Percentage of total
1	79.43774985511392
2	13.150507457247501
3	3.523400153883534
4	1.38919092031498
5	0.6450254662750647
6	0.3941614952214013
7	0.25348326938650373
8	0.17907151818401934
9	0.15608140741786755
>10	0.817718681692743
>50	0.045942970964574226
>100	0.007666804297932278
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	7.165058075303471E-6	3.5825290376517353E-6	1.0747587112955206E-5	3.5825290376517353E-6	3.5825290376517353E-6	3.5825290376517353E-6
2	7.165058075303471E-6	7.165058075303471E-6	1.4330116150606941E-5	1.0747587112955206E-5	5.7320464602427765E-5	7.165058075303471E-6
3	7.165058075303471E-6	2.8660232301213882E-5	1.4330116150606941E-5	2.8660232301213882E-5	9.314575497894511E-5	2.1495174225910412E-5
4	7.165058075303471E-6	3.5825290376517356E-5	1.4330116150606941E-5	3.5825290376517356E-5	1.1464092920485553E-4	3.940781941416909E-5
5	1.7912645188258678E-5	5.7320464602427765E-5	1.7912645188258678E-5	4.2990348451820824E-5	1.4330116150606943E-4	6.09029936400795E-5
6	1.7912645188258678E-5	6.448552267773123E-5	2.507770326356215E-5	4.657287748947256E-5	1.7912645188258676E-4	6.448552267773123E-5
7	1.7912645188258678E-5	7.523310979068645E-5	3.2242761338865616E-5	5.373793556477603E-5	2.1495174225910412E-4	8.239816786598991E-5
8	2.8660232301213882E-5	8.956322594129338E-5	3.5825290376517356E-5	5.373793556477603E-5	2.4719450359796975E-4	9.314575497894511E-5
9	2.8660232301213882E-5	9.314575497894511E-5	4.2990348451820824E-5	5.7320464602427765E-5	2.758547358991836E-4	9.314575497894511E-5
10-11	3.940781941416909E-5	9.314575497894511E-5	5.01554065271243E-5	6.09029936400795E-5	3.242188779074821E-4	1.0210207757307446E-4
12-13	5.01554065271243E-5	1.0389334209190033E-4	6.09029936400795E-5	6.985931623420885E-5	3.8691313606638745E-4	1.1822345824250726E-4
14-15	6.269425815890537E-5	1.1464092920485553E-4	7.344184527186058E-5	8.418943238481578E-5	4.621462458570739E-4	1.2897104535546246E-4
16-17	6.806805171538297E-5	1.4509242602489528E-4	8.060690334716405E-5	1.0747587112955206E-4	5.105103878653722E-4	1.6300507121315395E-4
18-19	7.165058075303471E-5	1.5942254217550224E-4	8.598069690364165E-5	1.3792736794959178E-4	5.624570589113225E-4	1.827089809202385E-4
20-21	7.165058075303471E-5	1.7017012928845741E-4	9.493701949777098E-5	1.504662195813729E-4	6.251513170702277E-4	1.9524783255201958E-4
22-23	7.881563882833818E-5	2.0420415514614892E-4	1.0210207757307446E-4	1.665876002508057E-4	6.663504010032228E-4	2.0778668418380066E-4
24-25	8.777196142246751E-5	2.1495174225910412E-4	1.0926713564837793E-4	1.8450024543906435E-4	7.523310979068644E-4	2.1495174225910412E-4
26-27	9.851954853542271E-5	2.3823818100384038E-4	1.271797808366366E-4	1.988303615896713E-4	8.651807625928941E-4	2.274905938908852E-4
28-29	1.003108130542486E-4	2.794372649368354E-4	1.3434483891194008E-4	2.274905938908852E-4	9.816129563165756E-4	2.43611974560318E-4
30-31	1.1284966468602966E-4	2.9914117464391993E-4	1.3613610343076593E-4	2.4182071004149214E-4	0.0011123752661908637	2.937673810874423E-4
32-33	1.1464092920485553E-4	3.1526255531335267E-4	1.433011615060694E-4	2.471945035979697E-4	0.0011876083759815503	3.098887617568751E-4
34-35	1.2359725179898485E-4	3.278014069451338E-4	1.5225748410019877E-4	2.6331588426740254E-4	0.0012646327502910627	3.4750531665221835E-4
36-37	1.2538851631781073E-4	3.4571405213339244E-4	1.719613938072833E-4	2.6868967782388016E-4	0.0013721086214206145	3.8691313606638745E-4
38-39	1.325535743931142E-4	3.672092263593029E-4	1.8450024543906435E-4	2.901848520497906E-4	0.0014383854086171717	4.2632095548055654E-4
40-41	1.4150989698724355E-4	3.976607231793426E-4	1.9524783255201958E-4	3.0809749723804925E-4	0.001544070015227898	4.836414200829843E-4
42-43	1.4688369054372116E-4	4.19155897405253E-4	2.0778668418380066E-4	3.1347129079452687E-4	0.0016676672670268827	5.463356782418896E-4
44-45	1.504662195813729E-4	4.2452969096173064E-4	2.1853427129675586E-4	3.224276133886562E-4	0.0018038033704576486	5.982823492878399E-4
46-47	1.5584001313785048E-4	4.3706854259351166E-4	2.3465565196618867E-4	3.367577295392631E-4	0.001880827744767161	6.556028138902676E-4
48-49	1.665876002508057E-4	4.621462458570739E-4	2.43611974560318E-4	3.439227876145666E-4	0.001959643383595499	7.30835923680954E-4
50-51	1.6837886476963156E-4	4.800588910453325E-4	2.543595616732732E-4	3.672092263593029E-4	0.0020384590224238374	7.809913302080783E-4
52-53	1.7375265832610917E-4	5.051365943088946E-4	2.7943726493683533E-4	3.7795681347225807E-4	0.0021154833967333498	8.311467367352026E-4
54-55	1.8091771640141264E-4	5.176754459406758E-4	2.937673810874423E-4	3.9407819414169086E-4	0.002190716506524036	9.045885820070632E-4
56-57	1.8450024543906435E-4	5.302142975724569E-4	3.098887617568751E-4	4.2094716192407893E-4	0.002260575822758245	9.547439885341875E-4
58-59	1.934565680331937E-4	5.373793556477603E-4	3.2421887790748204E-4	4.3706854259351166E-4	0.0023483477841807123	0.0010353508918813515
60-61	1.988303615896713E-4	5.588745298736708E-4	3.278014069451338E-4	4.478161297064669E-4	0.002462988713385568	0.001126705382341471
62-63	2.059954196649748E-4	5.803697040995812E-4	3.367577295392631E-4	4.5139865874411865E-4	0.002627785049117548	0.0012216424018392418
64-65	2.1495174225910412E-4	5.893260266937104E-4	3.4571405213339244E-4	4.621462458570738E-4	0.00278720759129305	0.0013201619503746644
66-67	2.221168003344076E-4	6.036561428443175E-4	3.582529037651735E-4	4.8185015556415843E-4	0.002953795191543856	0.0014276378215042165
68-69	2.3465565196618867E-4	6.054474073631433E-4	3.6900049087812875E-4	5.069278588277205E-4	0.0031024701466064028	0.0015655651894538082
70-71	2.454032390791439E-4	6.161949944760985E-4	3.7795681347225807E-4	5.266317685348051E-4	0.0032260673984053877	0.0016873711767339673
72-73	2.561508261920991E-4	6.305251106267054E-4	3.833306070287357E-4	5.499182072795413E-4	0.0034177327019197555	0.0017984295769011712
74-75	2.6152461974857663E-4	6.376901687020089E-4	3.8870440058521325E-4	5.678308524678E-4	0.0035843203021705614	0.0019130705061060267
76-77	2.830197939744871E-4	6.448552267773124E-4	4.0303451673582023E-4	5.749959105431036E-4	0.003781359399241407	0.00208503189991331
78-79	2.901848520497906E-4	6.556028138902676E-4	4.101995748111237E-4	5.893260266937104E-4	0.003996311141500511	0.00224982823564529
80-81	2.937673810874423E-4	6.62767871965571E-4	4.137821038487754E-4	5.982823492878398E-4	0.004187976445014879	0.0024307459520467025
82-83	2.9914117464391993E-4	6.77097988116178E-4	4.2273842644290473E-4	6.054474073631433E-4	0.004458457387357584	0.0026546540168999357
84-85	3.1168002627570096E-4	6.968018978232625E-4	4.3348601355585996E-4	6.233600525514019E-4	0.004711025684512032	0.002896474726941428
86-87	3.224276133886562E-4	7.290446591621282E-4	4.406510716311635E-4	6.484377558149641E-4	0.004918812368695832	0.0031365041724640942
88-89	3.331752005016114E-4	7.43374775312735E-4	4.478161297064669E-4	6.520202848526158E-4	0.0051552592851808465	0.0033317520050161137
>>END_MODULE
