##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674249
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26685044
Total Bases	2.6 Gbp
Sequences flagged as poor quality	0
Sequence length	64-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.649980078728746	37.0	35.0	37.0	29.0	37.0
2	34.10483411606891	37.0	34.0	37.0	27.0	37.0
3	34.11527502071947	37.0	34.0	37.0	27.0	37.0
4	34.056433633761294	37.0	34.0	37.0	27.0	37.0
5	34.06323538383523	37.0	34.0	37.0	27.0	37.0
6	34.108294818625744	37.0	34.0	37.0	27.0	37.0
7	34.120667516980674	37.0	34.0	37.0	27.0	37.0
8	34.0860855616352	37.0	34.0	37.0	27.0	37.0
9	33.99629863829342	37.0	34.0	37.0	26.0	37.0
10-11	33.984035008523875	37.0	34.0	37.0	26.0	37.0
12-13	33.86076655897588	36.0	33.0	37.0	26.0	37.0
14-15	33.93632628074363	36.5	33.0	37.0	26.0	37.0
16-17	33.83205729396586	36.0	33.0	37.0	26.0	37.0
18-19	33.75810171045624	36.0	33.0	37.0	25.5	37.0
20-21	33.74802111999516	36.0	33.0	37.0	26.0	37.0
22-23	33.628569471348825	36.0	33.0	37.0	25.0	37.0
24-25	33.62550901171458	36.0	33.0	37.0	25.0	37.0
26-27	33.51443345943143	36.0	33.0	37.0	25.0	37.0
28-29	33.471550318597934	36.0	33.0	37.0	24.5	37.0
30-31	33.349495339037105	36.0	33.0	37.0	24.0	37.0
32-33	33.260953420200465	36.0	32.0	37.0	24.0	37.0
34-35	33.18578172477437	36.0	32.0	37.0	24.0	37.0
36-37	33.13438788034226	36.0	32.0	37.0	23.0	37.0
38-39	33.16578040868136	36.0	32.0	37.0	24.0	37.0
40-41	32.97346099560488	36.0	32.0	37.0	23.0	37.0
42-43	32.90724161818882	36.0	32.0	37.0	23.0	37.0
44-45	32.88056407176994	36.0	32.0	37.0	23.0	37.0
46-47	32.71829748528801	36.0	31.5	37.0	22.5	37.0
48-49	32.73922533161272	36.0	31.0	37.0	22.0	37.0
50-51	32.624084768231974	36.0	31.0	37.0	21.5	37.0
52-53	32.403717677962234	36.0	31.0	37.0	21.0	37.0
54-55	32.31264514684705	36.0	31.0	37.0	20.5	37.0
56-57	32.24309150473951	36.0	30.5	37.0	20.5	37.0
58-59	32.13824760791101	36.0	30.5	37.0	20.5	37.0
60-61	32.00294427095567	36.0	30.0	37.0	19.5	37.0
62-63	31.918026573237054	36.0	30.0	37.0	19.0	37.0
64-65	31.82998348733044	36.0	30.0	37.0	19.0	37.0
66-67	31.74413813558757	36.0	29.5	37.0	19.0	37.0
68-69	31.684205787804487	36.0	29.0	37.0	18.5	37.0
70-71	31.485882599284942	36.0	29.0	37.0	18.0	37.0
72-73	31.352123058196504	36.0	29.0	37.0	17.5	37.0
74-75	31.200403805971206	36.0	28.5	37.0	17.0	37.0
76-77	31.146524000434233	36.0	28.0	37.0	17.0	37.0
78-79	31.11154254258168	36.0	28.0	37.0	16.5	37.0
80-81	30.902816549515485	35.0	28.0	37.0	16.0	37.0
82-83	30.81052748332182	35.0	27.5	37.0	16.0	37.0
84-85	30.652886137643122	35.0	27.0	37.0	15.5	37.0
86-87	30.42407841893439	35.0	26.5	37.0	14.5	37.0
88-89	30.24691042668509	35.0	26.0	37.0	14.0	37.0
90-91	30.12792419605843	35.0	26.0	37.0	14.0	37.0
92-93	30.071608116025818	35.0	26.0	37.0	14.0	37.0
94-95	29.93096379108702	35.0	25.0	37.0	13.0	37.0
96-97	29.890918326788043	35.0	25.0	37.0	13.0	37.0
98-99	30.43329239112444	35.0	26.5	37.0	15.5	37.0
100	33.32521360318033	36.0	31.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	144.0
20	11396.0
21	111066.0
22	341543.0
23	554536.0
24	672715.0
25	761466.0
26	857896.0
27	973744.0
28	1110653.0
29	1277662.0
30	1487793.0
31	1753223.0
32	2108368.0
33	2596457.0
34	3298375.0
35	4392596.0
36	4292009.0
37	83401.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	25.59076796160192	28.603524823758814	24.64052422378881	21.165182990850457
2	24.246256515391032	30.47674315479645	26.406429150401824	18.8705711794107
3	19.05721168841616	27.9646790256968	29.90784463459088	23.070264651296167
4	20.790447073563545	28.2568074187783	28.80093657788923	22.151808929768922
5	21.668386747952425	27.416976008249495	28.855039941888254	22.059597301909825
6	21.62333418487022	27.490710719674873	28.539109439858034	22.346845655596876
7	21.928316138625856	27.75000878227834	28.466204049610706	21.8554710294851
8	21.046723730881027	27.393728084545206	29.21803483081288	22.34151335376089
9	21.185710131812403	27.036242542141665	29.407026059857564	22.371021266188365
10-11	21.607415519864936	27.524431835797643	28.806997541548807	22.061155102788618
12-13	21.511263408775317	27.529610146820417	28.77008362409592	22.189042820308345
14-15	21.52301426643803	27.574875359720046	28.704629575374863	22.197480798467065
16-17	21.709663316844175	27.51408083332999	28.66547259605702	22.11078325376882
18-19	21.65294362876513	27.349234072334816	28.679049259571876	22.31877303932818
20-21	21.659466346784065	27.352376574124648	28.708494608166113	22.279662470925174
22-23	21.817005720489703	27.50550129252687	28.531689629992847	22.145803356990587
24-25	21.780152117372126	27.377927932199476	28.544773567021302	22.2971463834071
26-27	21.785153433077333	27.34778798938161	28.566489416134814	22.30056916140624
28-29	21.937090778510985	27.375169358414674	28.455689079200752	22.232050783873586
30-31	21.934405514481455	27.260022952016115	28.419493357578578	22.38607817592385
32-33	21.901803672261224	27.24665189874224	28.47916777391118	22.372376655085358
34-35	22.029416723907076	27.354143094672178	28.355423190756184	22.261016990664555
36-37	22.022469421266504	27.214487655625963	28.365427870642073	22.39761505246546
38-39	21.967069797827236	27.222354809580658	28.41628382652551	22.39429156606659
40-41	22.110429951820386	27.259757877223656	28.30172903847261	22.328083132483346
42-43	22.07293597671267	27.14901716747046	28.319867143759524	22.45817971205734
44-45	22.019107974123905	27.185110087976206	28.35872662893044	22.437055308969445
46-47	22.17160951815844	27.195874313041873	28.312088797026387	22.3204273717733
48-49	22.139580754470014	27.113053014462423	28.280131915130198	22.46723431593737
50-51	22.109792084726003	27.09438836087208	28.32386956040921	22.471949993992705
52-53	22.241127065978105	27.149140454108768	28.2266871624267	22.383045317486427
54-55	22.214584076711084	27.02875641399881	28.26110045166536	22.49555905762474
56-57	22.191001396540855	27.037224003260313	28.293434196387395	22.478340403811433
58-59	22.334671830749606	27.09805682366298	28.179364503987554	22.387906841599865
60-61	22.33210513204964	26.971231556446966	28.179292859397957	22.517370452105435
62-63	22.295619258081274	26.94078622077294	28.23644681518564	22.527147705960147
64-65	22.42962230479941	27.0125468573162	28.144261448291573	22.41356938959282
66-67	22.378677407754715	26.95233436490537	28.145752817670356	22.523235409669564
68-69	22.339963200267068	26.954569268935636	28.19946621757603	22.50600131322126
70-71	22.511195624149426	26.977413438047943	28.078206435847292	22.43318450195534
72-73	22.5072854559528	26.850221101983752	28.090569052499838	22.551924389563613
74-75	22.517169641567094	26.850580574941148	28.094206661846982	22.538043121644783
76-77	22.594492967744717	26.921074740615087	28.059473981389694	22.424958310250506
78-79	22.559890573725415	26.841900986856388	28.06566806118736	22.53254037823084
80-81	22.57657202688184	26.81880686921014	28.057284382629998	22.547336721278025
82-83	22.724588625842784	26.886555928239126	27.942751715294513	22.44610373062358
84-85	22.696488423535268	26.78119964590191	27.953246560543832	22.569065370018993
86-87	22.72834759390963	26.745720322571252	27.950104596974256	22.57582748654486
88-89	22.90308657772783	26.78852276725982	27.80359843277652	22.504792222235835
90-91	22.859305801236733	26.693787855857792	27.84521739981563	22.601688943089847
92-93	22.850346330068874	26.723546159971047	27.834085809355514	22.592021700604565
94-95	22.992914154788064	26.782830957612635	27.7311116119218	22.4931432756775
96-97	22.94708896014712	26.691616551362806	27.761411445440977	22.599883043049097
98-99	22.78287451914774	26.639833135671093	27.89605957444031	22.681232770740852
100	22.981082391242648	26.018452749787528	27.923112298751327	23.077352560218493
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	5.5
2	3.5
3	3.0
4	2.5
5	3.5
6	3.5
7	6.0
8	12.5
9	36.5
10	83.0
11	155.0
12	275.5
13	511.5
14	917.0
15	1711.5
16	3413.5
17	5934.5
18	9444.0
19	15337.0
20	24173.5
21	36002.5
22	51285.0
23	70494.0
24	94395.0
25	123199.5
26	155721.0
27	192062.0
28	231949.0
29	274521.0
30	321009.5
31	371176.0
32	425498.0
33	485566.5
34	553477.5
35	626572.0
36	704868.0
37	787742.5
38	870412.5
39	950605.0
40	1027024.5
41	1097352.0
42	1155551.5
43	1196821.5
44	1217401.5
45	1219320.5
46	1200758.5
47	1160352.0
48	1102139.5
49	1033272.0
50	958911.5
51	879148.0
52	795649.5
53	710215.0
54	629052.5
55	554230.0
56	484025.0
57	422143.5
58	368211.5
59	319914.5
60	278355.5
61	242441.5
62	210682.5
63	183031.5
64	158671.0
65	137370.0
66	118138.5
67	100209.5
68	84172.5
69	69566.0
70	56731.0
71	45540.5
72	35945.0
73	27767.0
74	20916.0
75	15558.0
76	11343.0
77	8087.5
78	5668.0
79	3945.5
80	2734.0
81	1812.0
82	1187.5
83	784.0
84	515.0
85	330.0
86	193.5
87	117.5
88	76.0
89	45.0
90	26.5
91	15.0
92	10.5
93	8.0
94	5.5
95	3.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06387098331185063
2	0.06887378563063265
3	0.1707885510700301
4	0.08891497424549871
5	0.04091055648999492
6	0.029188634652429275
7	0.04501023119916909
8	0.03246762493627517
9	0.06822548240879799
10-11	0.04097051516947096
12-13	0.09467475489266572
14-15	0.03927668247427286
16-17	0.026655380444566626
18-19	0.09645103077214337
20-21	0.029068717293477202
22-23	0.013441986455034511
24-25	0.019677689120542578
26-27	0.028293001877755946
28-29	0.0987257131747656
30-31	0.028663996207013938
32-33	0.01760536726115198
34-35	0.08759026217082498
36-37	0.015759764158530146
38-39	0.01694394807818192
40-41	0.09529307877476237
42-43	0.035952723180819934
44-45	0.042975383514451015
46-47	0.015385022411804904
48-49	0.0051433304738039785
50-51	0.018622791103511015
52-53	0.10548418057695538
54-55	0.1291004054555803
56-57	0.024832261846748315
58-59	0.07240760030225171
60-61	0.08117655717562242
62-63	0.05093302450616158
64-65	0.028652754491481344
66-67	0.0994489740628659
68-69	0.026719089545337405
70-71	0.037451700693757715
72-73	0.03286486288784825
74-75	0.028622790167798017
76-77	0.09253692258249421
78-79	0.03979012041814028
80-81	0.1659059023632972
82-83	0.03516959859702344
84-85	0.03892086523564221
86-87	0.057946990551732504
88-89	0.10964366997103564
90-91	0.06300458408851105
92-93	0.0823640423944358
94-95	0.08005117840967982
96-97	0.07733429812063448
98-99	0.09065109753554847
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
64	1.0
65	1.0
66	1.0
67	0.0
68	0.0
69	3.0
70	3.0
71	0.0
72	1.0
73	4.0
74	1.0
75	2.0
76	6.0
77	3.0
78	4.0
79	4.0
80	8.0
81	12.0
82	22.0
83	32.0
84	41.0
85	94.0
86	135.0
87	245.0
88	508.0
89	877.0
90	1701.0
91	3207.0
92	6519.0
93	13190.0
94	27179.0
95	59814.0
96	140496.0
97	342780.0
98	949121.0
99	3431370.0
100	2.1707659E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.87913771446138
#Duplication Level	Percentage of total
1	82.8147967494418
2	11.114312146234534
3	2.9725928518831157
4	1.0419585786794356
5	0.4942732959139647
6	0.29046351516578317
7	0.19097382192761495
8	0.14881331569085926
9	0.1186648875285606
>10	0.7753323753190823
>50	0.02257183212597898
>100	0.006279172419083073
>500	0.002294653889979987
>1k	0.006672803780225719
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.747417467252443E-6	7.494834934504886E-6	0.0	7.494834934504886E-6	4.122159213977687E-5	7.120093187779641E-5
2	7.494834934504886E-6	1.1242252401757328E-5	1.1242252401757328E-5	7.494834934504886E-6	8.993801921405863E-5	1.0118027161581596E-4
3	1.1242252401757328E-5	1.4989669869009773E-5	1.4989669869009773E-5	1.4989669869009773E-5	1.0867510655032085E-4	1.0118027161581596E-4
4	1.4989669869009773E-5	1.8737087336262216E-5	1.8737087336262216E-5	1.8737087336262216E-5	1.7612862096086482E-4	1.0867510655032085E-4
5	1.4989669869009773E-5	2.2484504803514657E-5	2.2484504803514657E-5	2.62319222707671E-5	2.1360279563338924E-4	1.1242252401757329E-4
6	1.4989669869009773E-5	2.2484504803514657E-5	2.9979339738019545E-5	2.9979339738019545E-5	2.360873004369039E-4	1.1616994148482573E-4
7	1.8737087336262216E-5	2.2484504803514657E-5	3.747417467252443E-5	3.372675720527199E-5	2.62319222707671E-4	1.1991735895207818E-4
8	2.2484504803514657E-5	2.62319222707671E-5	4.871642707428176E-5	4.122159213977687E-5	2.885511449784381E-4	1.2741219388658306E-4
9	2.62319222707671E-5	2.9979339738019545E-5	4.871642707428176E-5	4.496900960702931E-5	3.147830672492052E-4	1.4240186375559283E-4
10-11	3.1853048471645766E-5	3.1853048471645766E-5	5.433755327516042E-5	5.24638445415342E-5	3.841102903933754E-4	1.480229899564715E-4
12-13	3.372675720527199E-5	4.496900960702931E-5	5.995867947603909E-5	6.183238820966531E-5	4.3282671746765715E-4	1.5364411615735016E-4
14-15	3.372675720527199E-5	5.24638445415342E-5	6.745351441054398E-5	6.745351441054398E-5	4.721746008738078E-4	1.6301265982548126E-4
16-17	3.372675720527199E-5	5.621126200878664E-5	7.120093187779641E-5	7.120093187779641E-5	5.133961930135847E-4	1.836234558953697E-4
18-19	3.747417467252443E-5	5.621126200878664E-5	7.120093187779641E-5	9.930656288218974E-5	5.771022899568762E-4	1.9861312576437948E-4
20-21	4.309530087340309E-5	5.808497074241287E-5	7.86957668123013E-5	1.049276890830684E-4	6.42682095633794E-4	2.079816694325106E-4
22-23	4.496900960702931E-5	6.557980567691775E-5	8.056947554592751E-5	1.1616994148482573E-4	7.213778624460953E-4	2.1735021310064168E-4
24-25	5.0590135807907985E-5	8.056947554592751E-5	8.431689301317997E-5	1.3678073755471416E-4	7.850839593893868E-4	2.4920326157228746E-4
26-27	5.621126200878664E-5	9.930656288218974E-5	8.806431048043241E-5	1.461492812228453E-4	8.469163475990522E-4	2.735614751094283E-4
28-29	6.93272231441702E-5	1.0118027161581596E-4	9.930656288218974E-5	1.5739153362460262E-4	9.14369862009596E-4	3.0541452358107406E-4
30-31	7.682205807867508E-5	1.0305398034944217E-4	1.0305398034944217E-4	1.7612862096086482E-4	9.911919200882712E-4	3.5600465938898204E-4
32-33	8.244318427955374E-5	1.0680139781669463E-4	1.1242252401757329E-4	1.7987603842811725E-4	0.0010923721917040872	3.897314165942541E-4
34-35	8.619060174680619E-5	1.124225240175733E-4	1.1616994148482573E-4	1.8174974716174348E-4	0.001174815375983641	4.197107563322736E-4
36-37	9.368543668131107E-5	1.1804365021845196E-4	1.217910676857044E-4	1.8924458209624838E-4	0.001277869356333083	4.609323484720505E-4
38-39	1.0118027161581597E-4	1.1991735895207818E-4	1.2553848515295683E-4	2.0798166943251058E-4	0.0013734285017480202	5.059013580790798E-4
40-41	1.049276890830684E-4	1.2553848515295683E-4	1.292859026202093E-4	2.2109763056789414E-4	0.0014895984432328462	5.621126200878665E-4
42-43	1.1804365021845196E-4	1.3490702882108796E-4	1.386544462883404E-4	2.3233988296965147E-4	0.0016095158021849243	6.351872606992891E-4
44-45	1.2553848515295683E-4	1.4240186375559283E-4	1.480229899564715E-4	2.3983471790415634E-4	0.001759412500875022	6.76408852839066E-4
46-47	1.292859026202093E-4	1.5177040742372395E-4	1.5177040742372395E-4	2.4920326157228746E-4	0.0018587190637572117	7.251252799133477E-4
48-49	1.3490702882108796E-4	1.7050749475998615E-4	1.5739153362460262E-4	2.62319222707671E-4	0.001971141587774785	7.981999205247704E-4
50-51	1.4052815502196662E-4	1.7800232969449102E-4	1.6863378602635995E-4	2.6606664017492346E-4	0.002106048616595873	8.843905222715766E-4
52-53	1.4614928122284526E-4	1.9486570829712702E-4	1.7987603842811728E-4	2.79182601310307E-4	0.002257819024019597	9.612125803502517E-4
54-55	1.4989669869009772E-4	2.0423425196525814E-4	1.8737087336262215E-4	2.96045979912943E-4	0.002430200227513209	0.0010680139781669463
56-57	1.5364411615735016E-4	2.1735021310064168E-4	1.929919995635008E-4	3.0541452358107406E-4	0.0026269396445439625	0.0011691942497827623
58-59	1.5926524235822882E-4	2.2109763056789414E-4	1.9486570829712704E-4	3.110356497819528E-4	0.00282180535284109	0.001277869356333083
60-61	1.6113895109185505E-4	2.267187567687728E-4	2.098553781661368E-4	3.2602531965096257E-4	0.0030672611969461243	0.0014127763851541708
62-63	1.648863685591075E-4	2.3233988296965147E-4	2.173502131006417E-4	3.3726757205271985E-4	0.003290232536247645	0.0015720416275123998
64-65	1.667600772927337E-4	2.4170842663778257E-4	2.2109763056789414E-4	3.410149895199723E-4	0.0035150775842827916	0.0017275594524033763
66-67	1.6863378602635992E-4	2.5669809650679233E-4	2.267187567687728E-4	3.410149895199723E-4	0.0037586597196542	0.0018905721122288574
68-69	1.7612862096086482E-4	2.6606664017492346E-4	2.3608730043690393E-4	3.5038353318810343E-4	0.004047210864632639	0.0020254791410499453
70-71	1.8924458209624835E-4	2.754351838430546E-4	2.4920326157228746E-4	3.7099432925799184E-4	0.004341383135811955	0.002180996965940922
72-73	1.9111829082987458E-4	2.904248537120643E-4	2.529506790395399E-4	3.859839991270016E-4	0.0046580399117947865	0.0023964734703079372
74-75	2.004868344980057E-4	2.9791968864656924E-4	2.6794034890854966E-4	4.028473777296376E-4	0.005027160532319152	0.0026438030231465985
76-77	2.0610796069888437E-4	3.110356497819528E-4	2.735614751094283E-4	4.1783704759864736E-4	0.005394407444109892	0.0028873851585180074
78-79	2.079816694325106E-4	3.166567759828314E-4	2.754351838430546E-4	4.3470042620128335E-4	0.005744790977297996	0.0030841245755487603
80-81	2.1922392183426793E-4	3.204041934500839E-4	2.8293001877755945E-4	4.421952611357883E-4	0.006196354782101914	0.0033202118759856646
82-83	2.2484504803514657E-4	3.278990283845887E-4	2.885511449784381E-4	4.5156380480391935E-4	0.006586086198696169	0.00360501560349685
84-85	2.3796100917053013E-4	3.3164644585184123E-4	2.922985624456905E-4	4.534375135375456E-4	0.00711634577031239	0.003874829661139026
86-87	2.435821353714088E-4	3.372675720527199E-4	3.0166710611382165E-4	4.5905863973842427E-4	0.0076128785847233375	0.004092179874239668
88-89	2.4732955283866126E-4	3.6349949432348697E-4	3.166567759828314E-4	4.7404830960743406E-4	0.008071937224461763	0.004313277504807562
>>END_MODULE
