##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674251
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	45682306
Total Bases	4.5 Gbp
Sequences flagged as poor quality	0
Sequence length	61-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.00150395209909	37.0	35.0	37.0	30.0	37.0
2	34.52612155787407	37.0	35.0	37.0	28.0	37.0
3	34.64637908165144	37.0	35.0	37.0	29.0	37.0
4	34.615206158813436	37.0	35.0	37.0	28.0	37.0
5	34.579210165091055	37.0	35.0	37.0	28.0	37.0
6	34.482758050786664	37.0	35.0	37.0	28.0	37.0
7	34.59268198938994	37.0	35.0	37.0	28.0	37.0
8	34.56619727121481	37.0	35.0	37.0	28.0	37.0
9	34.543118182343946	37.0	35.0	37.0	28.0	37.0
10-11	34.52664264584192	37.0	35.0	37.0	28.0	37.0
12-13	34.43881300563067	37.0	35.0	37.0	28.0	37.0
14-15	34.46169914233314	37.0	35.0	37.0	28.0	37.0
16-17	34.3904415201807	37.0	34.0	37.0	28.0	37.0
18-19	34.3514554738108	37.0	34.0	37.0	27.5	37.0
20-21	34.32822035078527	37.0	34.0	37.0	27.0	37.0
22-23	34.20828510714849	37.0	34.0	37.0	27.0	37.0
24-25	34.19512772625795	37.0	34.0	37.0	27.0	37.0
26-27	34.15230176865415	37.0	34.0	37.0	27.0	37.0
28-29	34.112860983856635	37.0	34.0	37.0	26.5	37.0
30-31	34.0300252137009	37.0	34.0	37.0	26.0	37.0
32-33	33.971431422485544	37.0	34.0	37.0	26.0	37.0
34-35	33.939896576149195	37.0	34.0	37.0	26.0	37.0
36-37	33.88325974612577	37.0	34.0	37.0	26.0	37.0
38-39	33.85763825057343	37.0	34.0	37.0	26.0	37.0
40-41	33.77307626502042	37.0	34.0	37.0	25.0	37.0
42-43	33.7242685275126	37.0	33.0	37.0	25.0	37.0
44-45	33.642320004598716	37.0	33.0	37.0	25.0	37.0
46-47	33.54917698331604	37.0	33.0	37.0	24.5	37.0
48-49	33.60016592638734	37.0	33.0	37.0	25.0	37.0
50-51	33.50312798351291	37.0	33.0	37.0	24.0	37.0
52-53	33.39466655864527	37.0	33.0	37.0	24.0	37.0
54-55	33.30894333574141	37.0	33.0	37.0	23.5	37.0
56-57	33.252242575057394	36.5	33.0	37.0	23.5	37.0
58-59	33.1548326938662	36.0	32.5	37.0	23.0	37.0
60-61	33.060944384024744	36.0	32.0	37.0	23.0	37.0
62-63	32.98732428234521	36.0	32.0	37.0	22.5	37.0
64-65	32.947851775436256	36.0	32.0	37.0	22.0	37.0
66-67	32.877177871775245	36.0	32.0	37.0	22.0	37.0
68-69	32.831112254208364	36.0	32.0	37.0	22.0	37.0
70-71	32.69321558763414	36.0	32.0	37.0	21.0	37.0
72-73	32.58168941982599	36.0	31.5	37.0	21.0	37.0
74-75	32.47149350903063	36.0	31.0	37.0	20.0	37.0
76-77	32.478253622864216	36.0	31.0	37.0	20.0	37.0
78-79	32.419342870013864	36.0	31.0	37.0	20.0	37.0
80-81	32.303162891363556	36.0	31.0	37.0	20.0	37.0
82-83	32.19638785180106	36.0	31.0	37.0	19.0	37.0
84-85	32.094434243311625	36.0	30.5	37.0	19.0	37.0
86-87	31.95422460891876	36.0	30.0	37.0	18.5	37.0
88-89	31.84808584454879	36.0	30.0	37.0	18.0	37.0
90-91	31.747611707953887	36.0	30.0	37.0	18.0	37.0
92-93	31.688044132956545	36.0	30.0	37.0	17.5	37.0
94-95	31.597336567154734	36.0	29.5	37.0	17.0	37.0
96-97	31.5922418490876	36.0	29.0	37.0	17.0	37.0
98-99	31.94104467087476	36.0	30.0	37.0	19.5	37.0
100	34.02649878806115	36.0	32.0	37.0	26.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	316.0
20	18354.0
21	160935.0
22	468898.0
23	731313.0
24	866733.0
25	959616.0
26	1065389.0
27	1197816.0
28	1361394.0
29	1568322.0
30	1835081.0
31	2193356.0
32	2701731.0
33	3535466.0
34	5116653.0
35	8701898.0
36	1.2811116E7
37	387918.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.052094029718162	26.44808226039523	16.008789642137096	27.49103406774951
2	23.538049093253882	25.81896114262539	30.749984550703235	19.893005213417496
3	19.50894598406263	26.579394745653083	32.00831647595731	21.90334279432698
4	21.641058525982285	27.339858536302664	28.25254648628894	22.766536451426113
5	22.411456423530527	27.036646010633653	27.216133260864016	23.335764304971807
6	22.516764418409505	27.72286398668636	26.97914739233206	22.781224202572076
7	23.158527938880923	27.795602418756634	26.865665365588942	22.180204276773505
8	21.472838446840274	26.967026129101264	28.283837458725465	23.276297965333
9	21.762787438422478	25.927040331139995	28.92137072220993	23.388801508227598
10-11	22.89714428377447	26.619734559310626	27.32868222900041	23.154438927914494
12-13	23.014126082581367	26.780256960072883	26.980542563766146	23.225074393579607
14-15	22.78158129683751	26.997042428520757	27.140637642734898	23.080738631906833
16-17	23.06068477586184	27.00059300869909	26.974164435479516	22.964557779959556
18-19	23.0177379509012	26.822877223075697	26.976516734996043	23.182868091027064
20-21	22.862220026164383	26.898573308341717	27.159804416554916	23.07940224893898
22-23	23.05436274312195	27.076503995288732	26.96067874338135	22.908454518207964
24-25	23.03651413483311	26.87964793282135	27.04451758938266	23.039320342962885
26-27	22.895220116085465	26.86731671269594	27.24161629416481	22.995846877053783
28-29	23.13974176994492	26.965240010898505	26.934340982015847	22.960677237140732
30-31	23.140853735529447	26.794907775140903	26.92838358107423	23.135854908255425
32-33	22.962174619810156	26.853118194787772	27.10751607366586	23.077191111736216
34-35	23.166700166271898	26.990802716226813	26.900346118797046	22.942150998704243
36-37	23.183583692099457	26.784913169951547	26.92794633816074	23.103556799788254
38-39	23.029660799033508	26.821347252371186	27.080291805296593	23.068700143298713
40-41	23.268224846215798	26.891867786139635	26.816498386897543	23.023408980747025
42-43	23.230536208088793	26.73893154225398	26.854602911513993	23.175929338143238
44-45	23.037971016090395	26.795458449731225	27.075992471739685	23.090578062438695
46-47	23.266799349249737	26.873808892262385	26.881158079438993	22.97823367904888
48-49	23.266571913408946	26.726135571559528	26.875806985882424	23.131485529149103
50-51	23.11354995567155	26.751641975253904	27.025233086290573	23.10957498278397
52-53	23.329268129428588	26.81979609706055	26.82341919540049	23.027516578110372
54-55	23.303168659498397	26.696098787788415	26.828038210913117	23.172694341800074
56-57	23.15484606597477	26.723563462477657	27.02674103643438	23.094849435113193
58-59	23.380593913063407	26.779528660230696	26.822605879612283	23.017271547093618
60-61	23.394198697009543	26.61741069937304	26.8210417023756	23.167348901241823
62-63	23.22942035399243	26.642610139578515	26.982185565582085	23.14578394084697
64-65	23.434211918211144	26.757238815022937	26.76426919015169	23.04428007661423
66-67	23.412808401186417	26.602641398245453	26.809982470514647	23.174567730053482
68-69	23.254076297869098	26.643853962788278	26.995932054863815	23.10613768447881
70-71	23.494224792795755	26.704187106238358	26.774366046661825	23.02722205430406
72-73	23.49649761237145	26.555523638498652	26.7667254175765	23.181253331553403
74-75	23.367853169744258	26.58185689025427	26.897859745307713	23.152430194693764
76-77	23.541204732029538	26.68565091528446	26.72307718684327	23.050067165842734
78-79	23.53041240382749	26.54527373801514	26.75447989731687	23.1698339608405
80-81	23.41095860295579	26.569685861597552	26.89301932032565	23.126336215121004
82-83	23.62375543065024	26.648926318998118	26.683409192772395	23.04390905757925
84-85	23.637371752086402	26.496432377142497	26.671190585474875	23.195005285296226
86-87	23.51270382035574	26.498689271559684	26.810366904426353	23.178240003658228
88-89	23.738580352226897	26.58862811796846	26.557327431201784	23.11546409860286
90-91	23.73236260486715	26.43200446849057	26.60457144108821	23.231061485554072
92-93	23.604731767720445	26.48522044753066	26.742349778551734	23.167698006197163
94-95	23.81839610275739	26.548186871148943	26.52701716901437	23.106399857079293
96-97	23.805523167282818	26.429363957911395	26.52442707241486	23.240685802390928
98-99	23.575143112860825	26.457828672073823	26.730286265867882	23.23674194919747
100	24.116535392318585	25.89149430513053	26.35103147996584	23.64093882258504
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	17.5
2	14.0
3	16.5
4	20.0
5	22.0
6	34.5
7	68.5
8	137.5
9	277.5
10	594.0
11	1239.0
12	2382.5
13	4412.5
14	7936.5
15	13514.0
16	21562.5
17	32473.5
18	47110.0
19	66250.5
20	89923.5
21	118518.0
22	152181.0
23	191171.0
24	236250.0
25	285574.0
26	338938.0
27	397081.5
28	458640.0
29	522293.0
30	587391.0
31	654016.0
32	721681.0
33	789196.5
34	855038.5
35	920098.5
36	986663.5
37	1057351.5
38	1127982.0
39	1200265.5
40	1273986.5
41	1346514.0
42	1416273.5
43	1479205.5
44	1534165.0
45	1577819.0
46	1606905.0
47	1619395.5
48	1617073.0
49	1599289.0
50	1565983.0
51	1519354.5
52	1461209.0
53	1393724.5
54	1322171.5
55	1248000.5
56	1170327.0
57	1093775.5
58	1019725.0
59	946258.5
60	870377.0
61	794867.5
62	721449.5
63	644726.5
64	567195.5
65	491631.0
66	418300.0
67	349874.0
68	286436.5
69	229298.0
70	179983.5
71	138665.5
72	104523.5
73	76851.5
74	55410.0
75	39108.5
76	27334.5
77	18600.0
78	12466.5
79	8238.0
80	5455.5
81	3537.5
82	2209.0
83	1401.0
84	878.0
85	535.0
86	330.0
87	191.0
88	103.5
89	69.0
90	46.5
91	30.5
92	19.0
93	10.5
94	6.0
95	6.5
96	7.0
97	6.0
98	3.5
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07250290736198825
2	0.07219206491020834
3	0.11260377267294694
4	0.054900906272113324
5	0.029166653714897846
6	0.26928588062082504
7	0.05030175140458102
8	0.020743261077932448
9	0.060544229093864046
10-11	0.026907573361117102
12-13	0.08151514943225502
14-15	0.050467023271548506
16-17	0.03877759585954352
18-19	0.060655869692742746
20-21	0.027564282766285926
22-23	0.08437840243879108
24-25	0.07337195280816165
26-27	0.029639484486619395
28-29	0.07005775934340967
30-31	0.04727541556242804
32-33	0.01748269888126926
34-35	0.022166131455798224
36-37	0.018135030223736952
38-39	0.07890910760941008
40-41	0.042054575791335924
42-43	0.0167460898318049
44-45	0.116976362795696
46-47	0.06785778283609413
48-49	0.004173388269847849
50-51	0.019785559862061253
52-53	0.0069403238969591415
54-55	0.043918535986340095
56-57	0.01949989127081282
58-59	0.049125147053653555
60-61	0.06486318794852432
62-63	0.07470179098887414
64-65	0.0198435702788061
66-67	0.05772257336903221
68-69	0.018880398291688427
70-71	0.026040724926849116
72-73	0.02207748535176195
74-75	0.10377437605510005
76-77	0.027307095791910756
78-79	0.03146298995882306
80-81	0.028687315506685962
82-83	0.03507823577215199
84-85	0.039681864727823125
86-87	0.04464247112665242
88-89	0.04539516439159261
90-91	0.05619362569642326
92-93	0.056051564329891106
94-95	0.0638235863828377
96-97	0.04536102674527279
98-99	0.05653135897879557
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
61	1.0
62	0.0
63	1.0
64	1.0
65	1.0
66	1.0
67	1.0
68	2.0
69	4.0
70	4.0
71	3.0
72	2.0
73	3.0
74	6.0
75	8.0
76	5.0
77	12.0
78	13.0
79	20.0
80	14.0
81	18.0
82	28.0
83	45.0
84	72.0
85	111.0
86	190.0
87	364.0
88	598.0
89	1105.0
90	2009.0
91	4069.0
92	8080.0
93	16239.0
94	34486.0
95	75540.0
96	174473.0
97	423233.0
98	1157112.0
99	4368252.0
100	3.941618E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.15224077624177
#Duplication Level	Percentage of total
1	79.83285914382064
2	12.859118849686096
3	3.63727928318038
4	1.3638457276031992
5	0.7278470239228946
6	0.4412068177377805
7	0.27769124830594977
8	0.1913735678131329
9	0.14620809631886988
>10	0.5107800220810834
>50	0.007516882302301856
>100	0.004273337227663356
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.378062701125464E-6	2.189031350562732E-6	6.567094051688196E-6	1.5323219453939123E-5	3.064643890787825E-5	1.0726253617757387E-4
2	6.567094051688196E-6	2.189031350562732E-6	6.567094051688196E-6	1.9701282155064588E-5	6.348190916631923E-5	1.2039672428095027E-4
3	6.567094051688196E-6	1.3134188103376393E-5	6.567094051688196E-6	2.189031350562732E-5	9.412834807419748E-5	1.2477478698207572E-4
4	1.3134188103376393E-5	1.3134188103376393E-5	8.756125402250928E-6	3.283547025844098E-5	1.1382963022926207E-4	1.4009800643601485E-4
5	1.5323219453939123E-5	2.189031350562732E-5	1.5323219453939123E-5	3.502450160900371E-5	1.2477478698207572E-4	1.5323219453939124E-4
6	1.7512250804501856E-5	2.8457407557315518E-5	2.189031350562732E-5	4.159159566069191E-5	1.4009800643601485E-4	1.5542122588995397E-4
7	1.7512250804501856E-5	3.9402564310129177E-5	2.8457407557315518E-5	4.378062701125464E-5	1.4228703778657758E-4	1.641773512922049E-4
8	2.189031350562732E-5	4.8158689712380106E-5	2.8457407557315518E-5	5.034772106294284E-5	1.5323219453939124E-4	1.773115393955813E-4
9	2.8457407557315518E-5	5.4725783764068303E-5	2.8457407557315518E-5	5.4725783764068303E-5	1.773115393955813E-4	1.8606766479783223E-4
10-11	3.3929985933722346E-5	6.019836214047513E-5	3.7213532959566444E-5	6.348190916631923E-5	1.9920185290120862E-4	2.0686346262817817E-4
12-13	3.9402564310129177E-5	7.114351889328879E-5	5.1442236738224205E-5	7.223803456857016E-5	2.418879642371819E-4	2.3313183883493096E-4
14-15	4.925320538766147E-5	7.661609726969562E-5	5.910384646519377E-5	8.099415997082109E-5	2.9442471665068747E-4	2.517386053147142E-4
16-17	5.363126808878694E-5	8.427770699666518E-5	6.238739349103786E-5	9.850641077532294E-5	3.5681211014172536E-4	2.65967309093372E-4
18-19	5.910384646519376E-5	9.412834807419748E-5	6.895448754272606E-5	1.1164059887869934E-4	4.1044337823051226E-4	2.933302009754061E-4
20-21	6.348190916631923E-5	1.0178995780116704E-4	8.208867564610245E-5	1.1820769293038754E-4	4.531294895664856E-4	3.195985771821589E-4
22-23	7.114351889328879E-5	1.127351145539807E-4	9.850641077532294E-5	1.3134188103376392E-4	5.188004300833676E-4	3.5024501609003713E-4
24-25	8.099415997082109E-5	1.2039672428095026E-4	1.0835705185285524E-4	1.4994864751354713E-4	5.811878235744054E-4	3.6885178256982037E-4
26-27	8.427770699666518E-5	1.3024736535848256E-4	1.1711317725510617E-4	1.641773512922049E-4	6.359136073384737E-4	3.9074209607544764E-4
28-29	9.412834807419748E-5	1.3353091238432665E-4	1.3024736535848256E-4	1.893512118236763E-4	7.059626105564811E-4	4.12632409581075E-4
30-31	1.0288447347644841E-4	1.4666510048770304E-4	1.3790897508545211E-4	2.0139088425177135E-4	7.7710612944977E-4	4.531294895664856E-4
32-33	1.1054608320341797E-4	1.5651574156523535E-4	1.4666510048770304E-4	2.1780861938099186E-4	8.493441640183401E-4	4.925320538766147E-4
34-35	1.193022086056689E-4	1.6964992966861175E-4	1.5870477291579806E-4	2.287537761338055E-4	9.292438083138798E-4	5.275565554856184E-4
36-37	1.3571994373488938E-4	1.707444453438931E-4	1.6636638264276764E-4	2.4407699558774465E-4	0.0010069544212588568	5.702426668215918E-4
38-39	1.4557058481242166E-4	1.7840605507086268E-4	1.7512250804501857E-4	2.648727934180906E-4	0.0010956101909566475	6.238739349103785E-4
40-41	1.5870477291579806E-4	1.8497314912255085E-4	1.838786334472695E-4	2.8457407557315516E-4	0.0011645646784993735	6.720326246227588E-4
42-43	1.67460898318049E-4	1.9154024317423907E-4	1.9482379020008316E-4	2.9551923232596885E-4	0.0012641656049499777	7.278529240621085E-4
44-45	1.773115393955813E-4	2.0467443127761547E-4	2.0467443127761547E-4	3.02086326377657E-4	0.0013758062038286773	8.000909586306786E-4
46-47	1.8606766479783223E-4	2.1233604100458502E-4	2.1890313505627321E-4	3.163150301563148E-4	0.001471029067578156	8.493441640183401E-4
48-49	1.9810733722592724E-4	2.1780861938099186E-4	2.3094280748436826E-4	3.4477243771363034E-4	0.001572819025379323	9.215821985869103E-4
50-51	2.134305566798664E-4	2.254702291079614E-4	2.3641538586077507E-4	3.655682355439763E-4	0.0016932157496602732	0.001016805062336389
52-53	2.1780861938099186E-4	2.320373231596496E-4	2.4626602693830736E-4	3.8308048634847814E-4	0.0018114234425906607	0.001087948581229678
54-55	2.265647447832428E-4	2.3532087018549372E-4	2.561166680158396E-4	3.9512015877657316E-4	0.0019471433863255502	0.0011974001487578144
56-57	2.4517151126302603E-4	2.4079344856190054E-4	2.6487279341809054E-4	4.038762841788241E-4	0.0021102262219424737	0.0013199859043893274
58-59	2.550221523405583E-4	2.4298247991246327E-4	2.7253440314506014E-4	4.148214409316377E-4	0.002252513259729051	0.001444760691371403
60-61	2.637782777428092E-4	2.4954957396415144E-4	2.812905285473111E-4	4.312391760608582E-4	0.002410123516969568	0.0015793861194310112
62-63	2.769124658461856E-4	2.539276366652769E-4	2.9551923232596885E-4	4.389007857878278E-4	0.002616986979597746	0.0017216731572175887
64-65	2.8785762259899924E-4	2.5940021504168375E-4	3.1193696745518933E-4	4.509404582159228E-4	0.002799771097369734	0.0018683382577052918
66-67	2.9442471665068747E-4	2.681563404439347E-4	3.163150301563148E-4	4.69547224695706E-4	0.003002256497296787	0.0020007746544143373
68-69	3.064643890787825E-4	2.769124658461856E-4	3.3054373393497257E-4	4.8815399117548925E-4	0.0032189706010024976	0.002176991678134637
70-71	3.1740954583159617E-4	2.8347955989787383E-4	3.34921796636098E-4	4.969101165777402E-4	0.0034521024398374285	0.0023477361234785304
72-73	3.1850406150687755E-4	2.889521382742807E-4	3.436779220383489E-4	5.012881792788656E-4	0.003719164264606082	0.00255569410178199
74-75	3.250711555585657E-4	2.9770826367653155E-4	3.55717594466444E-4	5.122333360316792E-4	0.0039785644796477655	0.00274832886063151
76-77	3.382053436619421E-4	3.064643890787825E-4	3.644737198686949E-4	5.242730084597743E-4	0.004258760492519795	0.002950814260558563
78-79	3.502450160900372E-4	3.1850406150687755E-4	3.743243609462272E-4	5.395962279137134E-4	0.004530200379989574	0.003143449019408083
80-81	3.546230787911626E-4	3.2397663988328434E-4	3.8417500202375947E-4	5.428797749395575E-4	0.004806018330160478	0.003389715046346391
82-83	3.6009565716756944E-4	3.261656712338471E-4	3.8745854904960355E-4	5.527304160170899E-4	0.005157357861925797	0.003654587839764481
84-85	3.743243609462272E-4	3.283547025844098E-4	3.91836611750729E-4	5.614865414193408E-4	0.0055108864250416785	0.003912893539130884
86-87	3.8636403337432223E-4	3.34921796636098E-4	4.0168725282826133E-4	5.713371824968732E-4	0.005879738207611498	0.004169010207146723
88-89	3.962146744518545E-4	3.4586695338891167E-4	4.060653155293868E-4	5.844713706002495E-4	0.006223416129649847	0.004455773314070441
>>END_MODULE
