##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15674252
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	56538074
Total Bases	5.6 Gbp
Sequences flagged as poor quality	0
Sequence length	61-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.921265039909215	37.0	35.0	37.0	29.0	37.0
2	34.42521644794621	37.0	34.0	37.0	28.0	37.0
3	34.71927234380145	37.0	35.0	37.0	29.0	37.0
4	34.70278598453849	37.0	35.0	37.0	29.0	37.0
5	34.710348835016916	37.0	35.0	37.0	29.0	37.0
6	34.53872565591817	37.0	35.0	37.0	28.0	37.0
7	34.64805302706279	37.0	35.0	37.0	29.0	37.0
8	34.60468043180955	37.0	35.0	37.0	29.0	37.0
9	34.67658719679768	37.0	35.0	37.0	29.0	37.0
10-11	34.666287040481784	37.0	35.0	37.0	29.0	37.0
12-13	34.640492794289386	37.0	35.0	37.0	29.0	37.0
14-15	34.6564920658599	37.0	35.0	37.0	29.0	37.0
16-17	34.60499719180388	37.0	35.0	37.0	28.5	37.0
18-19	34.57121065885619	37.0	35.0	37.0	28.5	37.0
20-21	34.52175724097004	37.0	35.0	37.0	28.0	37.0
22-23	34.603408970033186	37.0	35.0	37.0	28.5	37.0
24-25	34.58051192193069	37.0	35.0	37.0	28.5	37.0
26-27	34.54647286888478	37.0	35.0	37.0	28.0	37.0
28-29	34.602051433517175	37.0	35.0	37.0	28.5	37.0
30-31	34.62181270094202	37.0	35.0	37.0	29.0	37.0
32-33	34.53315708101411	37.0	35.0	37.0	28.5	37.0
34-35	34.52185506000788	37.0	35.0	37.0	28.0	37.0
36-37	34.54294012562225	37.0	35.0	37.0	28.0	37.0
38-39	34.54002364848863	37.0	35.0	37.0	28.0	37.0
40-41	34.54208015646235	37.0	35.0	37.0	28.0	37.0
42-43	34.58487184229162	37.0	35.0	37.0	28.0	37.0
44-45	34.5789097714931	37.0	35.0	37.0	28.0	37.0
46-47	34.50440466896697	37.0	35.0	37.0	28.0	37.0
48-49	34.588214722347985	37.0	35.0	37.0	28.0	37.0
50-51	34.547188837737906	37.0	35.0	37.0	28.0	37.0
52-53	34.46264501334092	37.0	34.5	37.0	28.0	37.0
54-55	34.52234250144424	37.0	35.0	37.0	28.0	37.0
56-57	34.511709852373116	37.0	35.0	37.0	28.0	37.0
58-59	34.308049174084	37.0	34.0	37.0	27.5	37.0
60-61	34.47440947493189	37.0	34.0	37.0	28.0	37.0
62-63	34.493331648385094	37.0	35.0	37.0	28.0	37.0
64-65	34.4981371578177	37.0	35.0	37.0	28.0	37.0
66-67	34.46646616745066	37.0	34.0	37.0	28.0	37.0
68-69	34.47362270231342	37.0	34.0	37.0	28.0	37.0
70-71	34.43573742684306	37.0	34.0	37.0	28.0	37.0
72-73	34.38850554136501	37.0	34.0	37.0	28.0	37.0
74-75	34.366253811237364	37.0	34.0	37.0	28.0	37.0
76-77	34.40436223540053	37.0	34.0	37.0	28.0	37.0
78-79	34.367127557448555	37.0	34.0	37.0	28.0	37.0
80-81	34.31599472082149	37.0	34.0	37.0	27.5	37.0
82-83	34.28083870029809	37.0	34.0	37.0	27.0	37.0
84-85	34.21827975297803	37.0	34.0	37.0	27.0	37.0
86-87	34.164264177942826	37.0	34.0	37.0	27.0	37.0
88-89	34.106525384352054	37.0	34.0	37.0	27.0	37.0
90-91	34.09602464769516	37.0	34.0	37.0	27.0	37.0
92-93	34.07767421651262	37.0	34.0	37.0	27.0	37.0
94-95	33.93778695342101	37.0	34.0	37.0	26.0	37.0
96-97	34.06617853959828	37.0	34.0	37.0	27.0	37.0
98-99	34.092946301909436	37.0	34.0	37.0	26.5	37.0
100	34.79343553744261	37.0	34.0	37.0	29.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	257.0
20	12893.0
21	68007.0
22	134743.0
23	212060.0
24	302317.0
25	405161.0
26	543911.0
27	729428.0
28	973270.0
29	1285014.0
30	1690963.0
31	2241199.0
32	3049799.0
33	4375475.0
34	6898307.0
35	1.3008501E7
36	1.9954571E7
37	652198.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.9167566377519	34.18855003775557	18.56343288368166	24.331260440810873
2	24.865629953591245	29.965434130220014	29.540691352071512	15.62824456411723
3	15.998710244438923	28.708161763151747	32.4510157753625	22.84211221704683
4	17.953133955450618	30.029496981947606	30.60481722866264	21.412551833939137
5	20.832539315343123	28.541485024181007	29.701164586053586	20.92481107442228
6	20.501294926925834	27.947642182536992	30.33013513057576	21.22092775996142
7	21.26251096002481	29.28631200008397	28.85314134041973	20.598035699471495
8	19.28568433870243	27.540103584575842	31.135912781264974	22.038299295456756
9	20.31662447029195	26.72357057177766	31.124141082006528	21.835663875923863
10-11	21.49906972280067	27.827998850238973	28.96123058083005	21.711700846130306
12-13	21.544161100524313	28.11543156836872	28.452938479870443	21.887468851236523
14-15	21.546493699591338	28.221175964556995	28.454528200311696	21.777802135539975
16-17	21.753778396809484	28.247556635078404	28.351844831818184	21.646820136293925
18-19	21.655823173098437	28.021707603119722	28.37920451599135	21.94326470779049
20-21	21.69252414372715	27.98919311391247	28.43352457414087	21.88475816821951
22-23	21.879637184638707	28.255221546221783	28.133761264935398	21.731380004204116
24-25	21.816821655382952	28.074736142428524	28.140219554159717	21.96822264802881
26-27	21.823375460972873	28.02371085606648	28.26986048337125	21.88305319958939
28-29	21.97473328376146	28.13569362568189	28.077620033956315	21.811953056600338
30-31	21.908280357205996	27.963481028801855	28.07856202104749	22.04967659294466
32-33	21.866839926902692	27.981807604311776	28.189628419297026	21.961724049488502
34-35	22.00220581293657	28.144410914880673	28.0143186051855	21.839064666997253
36-37	21.959303322425033	27.955726948909643	28.0304264288042	22.054543299861123
38-39	21.923563755350667	27.939414856235146	28.1562732313568	21.98074815705739
40-41	22.058487322331985	28.06924240213078	27.983905037995388	21.888365237541844
42-43	21.980757707865408	27.932731066883182	27.992628932347426	22.093882292903984
44-45	21.924008844559772	27.95089736837932	28.124602057034938	22.000491730025974
46-47	22.044053938465957	28.08617107857311	27.985992739345793	21.883782243615137
48-49	21.990750137161207	27.926803679831046	27.992582160576045	22.0898640224317
50-51	21.958923714664706	27.912830991095966	28.10867957081718	22.01956572342215
52-53	22.06797456807303	28.053627996545373	27.958542575733247	21.91985485964835
54-55	21.991107877443216	27.91591492679517	27.974820663067046	22.118156532694563
56-57	21.950304544387865	27.923732783435877	28.111041059346192	22.014921612830065
58-59	22.07715350516839	28.03591790497853	27.97584285831699	21.911085731536094
60-61	22.017189021308216	27.901741752458275	27.967011725654643	22.114057500578863
62-63	21.984300127556907	27.897981436125647	28.084203835793804	22.03351460052364
64-65	22.100637634472616	28.060894485785624	27.920687616607953	21.9177802631338
66-67	22.018240065095128	27.913109006785742	27.95517880438035	22.113472123738777
68-69	21.95920935337505	27.9227711811182	28.105115657272083	22.01290380823466
70-71	22.108808329430314	28.035274106708485	27.947370283595347	21.90854728026585
72-73	22.02388079901393	27.892087760574295	27.95397590662111	22.130055533790667
74-75	21.984486598884452	27.89091091780289	28.074610452379957	22.0499920309327
76-77	22.099092897086877	28.040205480684854	27.93267133779183	21.928030284436435
78-79	22.021074434061937	27.90197086803862	27.96093505297416	22.116019644925277
80-81	21.985440342753925	27.891512540750313	28.088483863068532	22.034563253427233
82-83	22.106941764165708	28.050594818750625	27.921989806924287	21.92047361015938
84-85	22.056883677918616	27.880592535810223	27.93437460040769	22.12814918586347
86-87	22.01021598984469	27.903763701555174	28.04776496064297	22.03825534795717
88-89	22.12991664578684	28.0372622681869	27.893861348837206	21.938959737189048
90-91	22.066666479698977	27.889650760602702	27.92424804064569	22.11943471905263
92-93	22.02275281715293	27.878164355253244	28.063949303407558	22.035133524186268
94-95	22.15651994672018	28.006576367701406	27.903691726438794	21.933211959139616
96-97	22.072559858787592	27.883237783030896	27.909569388698802	22.134632969482713
98-99	22.02432288367945	27.862924087379863	28.038677323069685	22.074075705871003
100	22.468067416056357	27.41875049318413	27.696356470666082	22.416825620093427
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	3.0
2	1.5
3	4.5
4	4.5
5	3.0
6	3.0
7	3.5
8	7.0
9	47.0
10	103.0
11	264.0
12	588.5
13	1286.5
14	2563.0
15	4688.0
16	8233.0
17	13721.0
18	22023.0
19	34315.5
20	53386.0
21	79842.0
22	114069.0
23	157104.5
24	213565.0
25	286145.5
26	369554.5
27	462699.5
28	564714.0
29	677659.0
30	801507.0
31	931896.5
32	1071822.0
33	1223978.0
34	1383858.5
35	1543239.0
36	1705528.0
37	1865923.0
38	2012507.5
39	2147371.5
40	2268687.0
41	2369998.0
42	2445788.5
43	2489777.0
44	2499323.0
45	2475834.0
46	2415658.5
47	2317932.0
48	2190652.0
49	2044848.5
50	1883440.5
51	1716984.0
52	1550643.0
53	1383695.5
54	1222823.5
55	1073919.5
56	939969.5
57	819638.0
58	714695.5
59	620963.0
60	537193.5
61	464454.5
62	401224.5
63	348581.0
64	303162.0
65	261005.5
66	222562.0
67	186955.0
68	154597.0
69	124489.5
70	97212.0
71	74361.5
72	55515.5
73	40459.0
74	28710.5
75	19963.5
76	13616.0
77	9023.0
78	5906.5
79	3814.0
80	2360.0
81	1410.5
82	843.0
83	530.5
84	334.5
85	216.0
86	137.5
87	79.0
88	51.5
89	38.0
90	27.0
91	14.0
92	6.0
93	5.0
94	5.0
95	3.5
96	1.5
97	2.5
98	3.5
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027034879186015427
2	0.025978953580909035
3	4.05036789898432E-4
4	0.05471887846763227
5	0.0012947027519897476
6	0.18668127959222666
7	0.07200634390198718
8	0.1436430254062068
9	0.05316771137269373
10-11	0.03580330663545419
12-13	0.05130878706621666
14-15	0.0021675658778189015
16-17	0.03992000151968388
18-19	0.13623916513321624
20-21	0.2455849486489405
22-23	0.05577834151195175
24-25	0.1608721230935458
26-27	0.19455473492075445
28-29	0.07367513085076086
30-31	8.861285228782289E-4
32-33	0.22118369295706816
34-35	0.16952204632934614
36-37	0.12228927359640868
38-39	0.0787460499627207
40-41	0.14529500951871832
42-43	0.04606364907301229
44-45	0.016073239424462885
46-47	0.21081545862351095
48-49	0.0010382384090409588
50-51	0.07677746504063793
52-53	0.2119916571618623
54-55	0.07707372557473394
56-57	0.0472062419388393
58-59	0.4910142499725052
60-61	0.0010453132874671323
62-63	0.026646645939335835
64-65	0.0280784257403905
66-67	0.03131960741847108
68-69	9.993268163134106E-4
70-71	0.014305409617708357
72-73	0.20676343171688077
74-75	0.1565530344186565
76-77	0.01178764474421209
78-79	0.043978399052735935
80-81	0.07401585221917158
82-83	0.04567401518113139
84-85	0.12293054215214118
86-87	0.1353754739452689
88-89	0.0908229106332859
90-91	0.030597894587847483
92-93	0.06042351654181551
94-95	0.13771720480784427
96-97	0.002394690773137823
98-99	0.04253114016423171
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
61	1.0
62	1.0
63	1.0
64	2.0
65	3.0
66	2.0
67	2.0
68	3.0
69	5.0
70	3.0
71	5.0
72	4.0
73	9.0
74	8.0
75	8.0
76	10.0
77	12.0
78	23.0
79	46.0
80	75.0
81	153.0
82	161.0
83	205.0
84	242.0
85	370.0
86	521.0
87	532.0
88	745.0
89	933.0
90	1375.0
91	1843.0
92	2912.0
93	4774.0
94	8732.0
95	16084.0
96	38321.0
97	99332.0
98	368260.0
99	2297907.0
100	5.3694449E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.26303967772877
#Duplication Level	Percentage of total
1	67.28262962965927
2	18.797185777459614
3	6.983620971948038
4	2.8482179802932226
5	1.251397440158207
6	0.5770756119792748
7	0.39878560065913427
8	0.25838426159820443
9	0.19622367407872338
>10	1.3004611447631684
>50	0.08563166041622404
>100	0.01045739840004862
>500	0.002039981581602442
>1k	0.007888867005253267
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.768719606543371E-6	0.0	7.074878426173484E-6	8.843598032716855E-6	2.4762074491607196E-5
2	0.0	1.768719606543371E-6	3.537439213086742E-6	1.4149756852346969E-5	2.4762074491607196E-5	3.7143111737410794E-5
3	1.768719606543371E-6	5.306158819630114E-6	7.074878426173484E-6	3.0068233311237308E-5	3.537439213086742E-5	3.891183134395416E-5
4	3.537439213086742E-6	1.591847645889034E-5	1.0612317639260227E-5	3.537439213086742E-5	4.4217990163584277E-5	4.0680550950497535E-5
5	1.0612317639260227E-5	2.2993354885063825E-5	1.4149756852346969E-5	3.7143111737410794E-5	5.6599027409387875E-5	4.952414898321439E-5
6	1.591847645889034E-5	2.8299513704693937E-5	1.768719606543371E-5	3.891183134395416E-5	6.72113450486481E-5	5.483030780284451E-5
7	1.591847645889034E-5	3.0068233311237308E-5	2.1224635278520455E-5	4.244927055704091E-5	7.782366268790832E-5	6.72113450486481E-5
8	1.945591567197708E-5	3.0068233311237308E-5	2.2993354885063825E-5	5.306158819630113E-5	9.020469993371192E-5	7.605494308136496E-5
9	2.2993354885063825E-5	3.7143111737410794E-5	2.4762074491607196E-5	6.0136466622474616E-5	9.551085875334204E-5	1.043544567860589E-4
10-11	3.0068233311237308E-5	3.891183134395416E-5	2.6530794098150567E-5	6.809570485191978E-5	1.0789189599914563E-4	1.1231369501550406E-4
12-13	3.6258751934139104E-5	4.510234996685597E-5	3.979619114722585E-5	7.693930288463665E-5	1.282321714743944E-4	1.3530704990056787E-4
14-15	4.156491075376922E-5	5.394594799957282E-5	4.86397891799427E-5	9.285777934352698E-5	1.4149756852346968E-4	1.5210988616272994E-4
16-17	4.510234996685597E-5	6.278954603228967E-5	5.7483387212659565E-5	1.0523881658933059E-4	1.591847645889034E-4	1.7156580183470702E-4
18-19	4.6871069573399334E-5	6.986442445846316E-5	6.632698524537641E-5	1.229260126547643E-4	1.786406802608805E-4	1.9809659593285757E-4
20-21	5.129286858975776E-5	7.517058327809328E-5	7.163314406500653E-5	1.3884448911365464E-4	1.9986531553940095E-4	2.2197431062119307E-4
22-23	5.394594799957282E-5	8.312982150753844E-5	7.95923822944517E-5	1.5653168517908834E-4	2.1666815180156296E-4	2.4673638511280027E-4
24-25	5.571466760611619E-5	8.932034013044024E-5	8.312982150753844E-5	1.786406802608805E-4	2.387771468833551E-4	2.697297399978641E-4
26-27	5.925210681920293E-5	9.462649895007036E-5	8.666726072062517E-5	1.954435165230425E-4	2.582330625553322E-4	2.9095437527638457E-4
28-29	6.0136466622474616E-5	9.904829796642878E-5	9.993265776970047E-5	2.0340275475248766E-4	2.759202586207659E-4	3.1571644976799174E-4
30-31	6.632698524537641E-5	1.0700753619587394E-4	1.043544567860589E-4	2.2020559101464972E-4	2.9537617429274297E-4	3.4313160366941404E-4
32-33	6.898006465519147E-5	1.105449754089607E-4	1.0612317639260227E-4	2.3347098806372497E-4	3.051041321287315E-4	3.687780379642929E-4
34-35	7.074878426173484E-5	1.2204165285149261E-4	1.0877625580241732E-4	2.4850510471934365E-4	3.2721312721052366E-4	4.014993506853453E-4
36-37	7.251750386827822E-5	1.282321714743944E-4	1.1408241462204744E-4	2.6619230078477735E-4	3.5197520170213083E-4	4.2803014478349583E-4
38-39	7.517058327809328E-5	1.3442269009729618E-4	1.2115729304822092E-4	2.8387949685021105E-4	3.7673727619373805E-4	4.5190785947183136E-4
40-41	7.782366268790832E-5	1.4503500773655641E-4	1.3088525088420945E-4	3.095259311450899E-4	4.0680550950497537E-4	4.8639789179942707E-4
42-43	8.136110190099507E-5	1.5122552635945825E-4	1.3972884891692633E-4	3.1571644976799174E-4	4.333363036031259E-4	5.306158819630113E-4
44-45	8.755162052389686E-5	1.591847645889034E-4	1.459193675398281E-4	3.236756879974369E-4	4.598670977012765E-4	5.597997554709769E-4
46-47	9.19734195402553E-5	1.6449092340853352E-4	1.4945680675291487E-4	3.422472438661423E-4	4.7843865356998186E-4	6.057864652411046E-4
48-49	9.639521855661373E-5	1.6714400281834857E-4	1.5476296557254495E-4	3.5905008012830435E-4	4.943571300288722E-4	6.376234181588853E-4
50-51	1.0081701757297215E-4	1.7333452144125037E-4	1.6183784399871845E-4	3.749685565871947E-4	5.155817653073927E-4	6.676916514701226E-4
52-53	1.0966061560568901E-4	1.786406802608805E-4	1.6625964301507688E-4	3.90887033046085E-4	5.385751201924565E-4	7.012973239944466E-4
54-55	1.1850421363840586E-4	1.8129375967069553E-4	1.7068144203143533E-4	4.085742291115187E-4	5.624528348807921E-4	7.45515314158031E-4
56-57	1.2557909206457933E-4	1.8394683908051058E-4	1.71565801834707E-4	4.21839626160594E-4	5.827931103560408E-4	8.083048601903206E-4
58-59	1.3000089108093779E-4	1.954435165230425E-4	1.7952504006415218E-4	4.3598938301294093E-4	6.10208264257463E-4	8.595977287800783E-4
60-61	1.3265397049075283E-4	2.078245537688461E-4	1.8394683908051058E-4	4.466017006522012E-4	6.323172593392552E-4	9.05584438550206E-4
62-63	1.3796012931038294E-4	2.1224635278520453E-4	1.8836863809686903E-4	4.660576163241783E-4	6.676916514701226E-4	9.533398679268771E-4
64-65	1.4326628813001306E-4	2.1666815180156296E-4	1.963278763263142E-4	4.8020737317652524E-4	7.048347632075334E-4	0.0010072858159264498
66-67	1.468037273430998E-4	2.1755251160483465E-4	1.9809659593285757E-4	4.934727702256005E-4	7.402091553384008E-4	0.0010815720394012716
68-69	1.4768808714637149E-4	2.2020559101464972E-4	2.0694019396557442E-4	5.067381672746757E-4	7.525901925842045E-4	0.001141708506023746
70-71	1.5122552635945825E-4	2.263961096375515E-4	2.1666815180156296E-4	5.155817653073927E-4	7.746991876659966E-4	0.001194770094220047
72-73	1.5741604498236003E-4	2.3435534786699666E-4	2.2816482924409488E-4	5.376907603891848E-4	8.003456219608754E-4	0.0012416411637934466
74-75	1.6183784399871848E-4	2.4054586648989846E-4	2.387771468833551E-4	5.562623162578902E-4	8.224546170426676E-4	0.0013053150696290079
76-77	1.7156580183470702E-4	2.458520253095286E-4	2.4850510471934365E-4	5.757182319298673E-4	8.649038875997086E-4	0.0013654515362514826
78-79	1.786406802608805E-4	2.520425439324304E-4	2.555799831455171E-4	5.942897877985727E-4	8.923190415011307E-4	0.001417628764644512
80-81	1.8217811947396722E-4	2.555799831455171E-4	2.6000178216187557E-4	6.084395446509196E-4	9.321152326483566E-4	0.0014715747126440846
82-83	1.8571555868705396E-4	2.626548615716906E-4	2.635392213749623E-4	6.217049416999949E-4	9.568773071399637E-4	0.0015449765763156345
84-85	1.901373577034124E-4	2.697297399978641E-4	2.688453801945924E-4	6.385077779621569E-4	9.887142600577443E-4	0.0016077661223479244
86-87	1.945591567197708E-4	2.7768897822730927E-4	2.714984596044075E-4	6.57963693634134E-4	0.0010258573717951552	0.0016687869487736705
88-89	1.9898095573612926E-4	2.8476385665348274E-4	2.7857333803058096E-4	6.676916514701226E-4	0.0010665379227456527	0.0017474949712648507
>>END_MODULE
