##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR20982505
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	47221
Total Bases	28.4 Mbp
Sequences flagged as poor quality	0
Sequence length	602
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.78158022913534	34.0	34.0	34.0	34.0	34.0
2	33.78943690307278	34.0	34.0	34.0	34.0	34.0
3	33.89019715804409	34.0	34.0	34.0	34.0	34.0
4	33.782215539696324	34.0	34.0	34.0	34.0	34.0
5	33.82173185658923	34.0	34.0	34.0	34.0	34.0
6	37.67738929713475	38.0	38.0	38.0	37.0	38.0
7	37.7553207259482	38.0	38.0	38.0	38.0	38.0
8	37.651150970966306	38.0	38.0	38.0	38.0	38.0
9	37.5290019271087	38.0	38.0	38.0	37.0	38.0
10-19	37.342783930878205	38.0	38.0	38.0	36.4	38.0
20-29	37.5167192562631	38.0	38.0	38.0	37.0	38.0
30-39	37.39884585248088	38.0	38.0	38.0	36.7	38.0
40-49	37.26646407318778	38.0	38.0	38.0	36.8	38.0
50-59	37.20907223481079	38.0	38.0	38.0	36.3	38.0
60-69	37.36087969335677	38.0	38.0	38.0	36.7	38.0
70-79	37.12742423921561	38.0	37.8	38.0	35.9	38.0
80-89	37.22106689820207	38.0	38.0	38.0	36.5	38.0
90-99	37.010330149721526	38.0	37.8	38.0	35.7	38.0
100-109	36.871972215751455	38.0	37.8	38.0	35.6	38.0
110-119	36.79286758010208	38.0	37.2	38.0	35.2	38.0
120-129	36.96305457317718	38.0	37.9	38.0	35.7	38.0
130-139	36.950585544567026	38.0	37.8	38.0	35.9	38.0
140-149	36.671639736557886	38.0	37.3	38.0	34.4	38.0
150-159	36.77154232227187	38.0	37.7	38.0	34.6	38.0
160-169	36.803731390694814	38.0	37.9	38.0	34.9	38.0
170-179	36.727356472755766	38.0	37.8	38.0	34.4	38.0
180-189	36.5092607102772	38.0	37.3	38.0	33.7	38.0
190-199	36.594811630418675	38.0	37.6	38.0	34.3	38.0
200-209	36.0647084983376	38.0	36.9	38.0	32.5	38.0
210-219	36.0894707863027	38.0	36.9	38.0	32.7	38.0
220-229	35.34572965417928	38.0	35.8	38.0	28.5	38.0
230-239	35.49514622731412	38.0	36.4	38.0	28.8	38.0
240-249	35.3834353359734	38.0	36.1	38.0	28.2	38.0
250-259	34.61157959382478	37.6	34.4	38.0	25.6	38.0
260-269	34.796001778869574	37.8	35.0	38.0	25.8	38.0
270-279	34.46860506977828	37.5	34.3	38.0	24.3	38.0
280-289	33.79268334003939	37.1	33.9	38.0	22.6	38.0
290-299	32.492291565193455	36.2	30.5	37.7	20.2	37.9
300-309	33.66618877194468	34.9	33.2	35.8	30.1	36.0
310-319	37.07070159462951	38.0	38.0	38.0	35.8	38.0
320-329	36.98033713813769	38.0	37.8	38.0	35.7	38.0
330-339	36.917788695707415	38.0	37.8	38.0	35.5	38.0
340-349	37.04209144236674	38.0	37.8	38.0	35.8	38.0
350-359	36.768581775057704	38.0	37.7	38.0	35.1	38.0
360-369	36.86533322038923	38.0	38.0	38.0	34.9	38.0
370-379	36.70145274348277	38.0	37.4	38.0	34.8	38.0
380-389	36.79309629190403	38.0	37.9	38.0	34.8	38.0
390-399	36.51741386247644	38.0	37.1	38.0	34.1	38.0
400-409	36.695997543465836	38.0	37.5	38.0	34.8	38.0
410-419	36.60681264691557	38.0	37.1	38.0	34.3	38.0
420-429	36.569414031892585	38.0	37.2	38.0	34.5	38.0
430-439	36.41116240655641	38.0	37.2	38.0	33.7	38.0
440-449	36.33169140848351	38.0	37.0	38.0	33.8	38.0
450-459	36.38806463226107	38.0	37.1	38.0	33.8	38.0
460-469	35.7491582135067	37.9	36.2	38.0	30.6	38.0
470-479	36.043643717837405	38.0	36.8	38.0	32.2	38.0
480-489	35.79097223692848	38.0	36.9	38.0	31.0	38.0
490-499	35.43265072743059	37.9	35.9	38.0	29.3	38.0
500-509	35.14438914889562	38.0	35.7	38.0	27.5	38.0
510-519	34.72968806251456	37.4	35.3	38.0	26.2	38.0
520-529	34.10870163698354	36.9	33.9	38.0	24.0	38.0
530-539	31.946983333686283	35.9	29.6	37.2	19.9	37.2
540-549	31.95139662438322	36.0	29.6	37.3	19.7	37.5
550-559	30.48191482603079	34.6	25.8	37.0	17.7	37.4
560-569	28.676629042163444	31.6	23.1	36.8	15.4	37.2
570-579	28.665525931259396	31.2	23.5	37.0	15.8	37.0
580-589	25.785724571694793	27.8	19.3	33.8	11.1	36.6
590-599	24.05678617564219	25.8	17.2	30.6	9.0	36.2
600-602	18.84773723555198	18.666666666666668	13.0	24.333333333333332	7.0	29.666666666666668
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	8.0
22	19.0
23	62.0
24	109.0
25	187.0
26	350.0
27	496.0
28	700.0
29	927.0
30	1200.0
31	1585.0
32	1995.0
33	2786.0
34	3866.0
35	6453.0
36	14205.0
37	12273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	0.021177018699307514	99.9449397513818	0.027530124309099766	0.006353105609792253
8	0.002117701869930751	0.012706211219584505	0.008470807479723005	99.97670527943076
9	0.010588509349653757	0.006353105609792253	99.91317422333283	0.06988416170771479
10-19	39.965904999894114	20.011647360284616	10.01588276402448	30.006564875796787
20-29	41.20857245716948	28.776603629741004	10.011435590097625	20.00338832299189
30-39	22.781813176341036	22.673810380974565	30.026683043561125	24.51769339912327
40-49	39.17070794773512	31.207725376421507	11.477732364837678	18.143834311005698
50-59	18.161199466339127	35.38912771859978	21.198619258380806	25.25105355668029
60-69	36.41070710065437	29.924821583617454	0.8847758412570678	32.779695474471104
70-79	51.02983841934733	9.61923720378645	19.325511954427053	20.025412422439167
80-89	35.15088625823257	36.82492958641282	24.19474386395883	3.8294402913957777
90-99	30.859786959191887	17.094301264268015	32.68990491518604	19.356006861354057
100-109	14.02193939137248	23.00756019567565	41.010779102517944	21.959721310433917
110-119	51.10184028292497	31.52411003578916	16.10512272082336	1.2689269604625062
120-129	29.38967832108596	48.94707863027043	15.906058745049872	5.75718430359374
130-139	23.680565849939644	31.883907583490394	30.08280214311429	14.352724423455665
140-149	13.080832680375257	21.617924228627096	41.977933546515324	23.323309544482328
150-159	27.2965417928464	24.707439486669067	24.886173524491223	23.10984519599331
160-169	23.77798013595646	47.39416784905021	6.333199212214905	22.494652802778425
170-179	22.014781559052114	29.559729781241394	16.98651023908854	31.438978420617946
180-189	26.97528641917791	17.68683424747464	18.563139281251985	36.77474005209547
190-199	44.66614430020542	13.475572308930348	5.579932657080536	36.2783507337837
200-209	20.925859257533723	38.34374536752716	27.421486203172318	13.308909171766798
210-219	49.560365091802375	21.77865780055484	10.429469939221956	18.23150716842083
220-229	30.084708074797227	18.781262573854853	32.1346434848902	18.99938586645772
230-239	39.40301984286652	10.668134939963151	37.12987865568285	12.798966561487473
240-249	29.06884648779145	37.09112471146312	18.343321827153172	15.496706973592259
250-259	43.9908091738845	14.373901442154974	22.974100506130746	18.66118887782978
260-269	12.96266491603312	31.899578577327887	35.80335020435823	19.334406302280765
270-279	35.74045445882128	39.72236928485208	19.6514262722094	4.885749984117236
280-289	19.03390440693759	9.832489782088476	27.535630333961585	43.59797547701235
290-299	30.44154083988056	41.996569322970714	9.000656487579677	18.561233349569047
300-309	36.885072319518855	20.582791554604942	21.617288918066116	20.914847207810084
310-319	33.580186781304924	26.5168039643379	14.30486436119523	25.59814489316194
320-329	0.10228500031765528	24.531034920903835	45.37260964401432	29.994070434764193
330-339	19.922492111560537	7.196586264585672	44.09288240401516	28.78803921983863
340-349	10.610956989475023	10.062683975349952	35.181592935346565	44.144766099828466
350-359	29.137036488003222	21.917579043222297	11.997204633531691	36.9481798352428
360-369	26.47063806357341	23.26846106605112	31.016073357192774	19.244827513182695
370-379	38.4894432561784	33.99419749687639	6.063403993985727	21.452955252959487
380-389	20.0122826708456	0.18445183287096845	22.201774634167002	57.601490862116435
390-399	27.60000847080748	15.160204146460263	39.16986086698714	18.069926515745113
400-409	2.3794498210541923	25.591156476991166	37.102772071747744	34.9266216302069
410-419	13.15262277376591	18.367463628470386	39.623896147900304	28.85601744986341
420-429	8.495584591601196	20.62027487770272	16.123758497278754	54.76038203341733
430-439	24.033163211283114	34.36077169056141	28.47949005738972	13.126575040765761
440-449	0.8644459033057326	11.854683297685352	27.610173439783146	59.67069735922577
450-459	9.216662078312615	13.408017619279558	34.82476017026323	42.5505601321446
460-469	11.783952055229664	31.095487177315178	21.171936214819677	35.94862455263548
470-479	17.31538933948879	25.63266343364181	44.17250799432456	12.879439232544843
480-489	13.936172465640286	17.767942229092988	27.75756548993033	40.5383198153364
490-499	41.98343957137714	16.415366044768216	22.02198174540988	19.57921263844476
500-509	22.817602337942862	25.601533216153832	32.28415323690731	19.296711208995998
510-519	9.75201711103111	28.39626437390144	31.718303297261812	30.133415217805638
520-529	19.1529192520277	35.603862688210754	10.101437919569683	35.14178014019186
530-539	23.783697931005275	8.207153596916626	19.472268694013255	48.53687977806484
540-549	10.117532453781157	20.542978759450246	29.3485949048093	39.9908938819593
550-559	21.243514538023337	33.58505749560577	24.759534952669362	20.411893013701533
560-569	21.31466932085301	27.137714152601593	25.858410452976432	25.689206073568965
570-579	10.085766925732194	18.15696406259927	19.98687024840643	51.77039876326211
580-589	29.16795493530421	19.232544842337095	20.48813028101904	31.111369941339657
590-599	30.245229876537984	9.809406831706232	20.31066686431884	39.63469642743694
600-602	46.753210083084504	22.990477400591544	28.55226841165301	1.7040441046709445
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.14285714285714285
46	1.571428571428571
47	77.85714285714306
48	177.28571428571166
49	67.7142857142862
50	951.7142857142245
51	2158.999999999841
52	2206.428571428477
53	1354.9999999998472
54	594.5714285714486
55	462.85714285717324
56	119.28571428571331
57	24.42857142857138
58	2.8571428571428563
59	1.2857142857142854
60	1.9999999999999993
61	1.2857142857142854
62	0.0
63	0.42857142857142855
64	0.9999999999999998
65	0.14285714285714285
66	0.14285714285714285
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	0.0
350-359	0.0
360-369	0.0
370-379	0.0
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-479	0.0
480-489	0.0
490-499	0.0
500-509	0.0
510-519	0.0
520-529	0.0
530-539	0.0
540-549	0.0
550-559	0.0
560-569	0.0
570-579	0.0
580-589	0.0
590-599	0.0
600-602	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
602	47221.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.1710891340717056
#Duplication Level	Percentage of total
1	0.6586052815484635
2	0.39389254780711974
3	0.31765528048961267
4	0.21177018699307512
5	0.1694161495944601
6	0.1524745346350141
7	0.1482391308951526
8	0.05082484487833802
9	0.05717795048813028
>10	0.6480167721988099
>50	0.10800279536646831
>100	1.0736748480548908
>500	0.0
>1k	22.964359077529064
>5k	30.183604752122996
>10k+	42.862285847398404
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGTGCAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGC	20240	42.862285847398404	No Hit
TGTGCAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGA	7569	16.028885453505858	No Hit
TGTGCAACTCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGA	6684	14.154719298617142	No Hit
TGTGCAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC	3821	8.0917388450054	No Hit
TGTGCAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGC	3613	7.651256856059804	No Hit
TGTGCAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGA	1823	3.860570508883759	No Hit
TGTGCAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGA	1587	3.360792867580102	No Hit
TGTGCAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGA	347	0.7348425488659707	No Hit
TGTGCAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGC	160	0.3388322991889202	No Hit
TGTGCAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGG	51	0.10800279536646831	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0	0.0
260-269	0.0010588509349653756	0.0	0.0	0.0	0.0	0.0
270-279	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
280-289	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
290-299	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
300-309	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
310-319	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
320-329	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
330-339	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
340-349	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
350-359	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
360-369	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
370-379	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
380-389	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
390-399	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
400-409	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
410-419	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
420-429	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
430-439	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
440-449	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
450-459	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
460-469	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
470-479	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
480-489	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
490-499	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
500-509	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
510-519	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
520-529	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
530-539	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
540-549	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
550-559	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
560-569	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
570-579	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
580-589	0.008470807479723006	0.0	0.0	0.0	0.0	0.0
590-591	0.008470807479723005	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
