##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR20982509
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49582
Total Bases	29.8 Mbp
Sequences flagged as poor quality	0
Sequence length	602
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.872131015287806	34.0	34.0	34.0	34.0	34.0
2	33.8147714896535	34.0	34.0	34.0	34.0	34.0
3	37.60737767738292	38.0	38.0	38.0	37.0	38.0
4	37.66971884958251	38.0	38.0	38.0	37.0	38.0
5	37.54243475454802	38.0	38.0	38.0	37.0	38.0
6	37.246924287039654	38.0	38.0	38.0	37.0	38.0
7	37.44201524746884	38.0	38.0	38.0	37.0	38.0
8	37.70085918276794	38.0	38.0	38.0	37.0	38.0
9	37.46914202734863	38.0	38.0	38.0	37.0	38.0
10-19	37.46714331813964	38.0	38.0	38.0	36.9	38.0
20-29	37.39438102537211	38.0	38.0	38.0	36.7	38.0
30-39	37.432566253882456	38.0	38.0	38.0	36.9	38.0
40-49	37.24933241902303	38.0	38.0	38.0	36.1	38.0
50-59	37.3813178169497	38.0	38.0	38.0	36.7	38.0
60-69	37.271604614577875	38.0	38.0	38.0	36.1	38.0
70-79	37.10903150336815	38.0	37.8	38.0	35.7	38.0
80-89	37.08486950909604	38.0	37.9	38.0	36.1	38.0
90-99	37.05364648461135	38.0	38.0	38.0	35.7	38.0
100-109	37.30882578355049	38.0	38.0	38.0	36.9	38.0
110-119	36.941383969989104	38.0	37.8	38.0	35.6	38.0
120-129	37.03439352991005	38.0	37.9	38.0	35.9	38.0
130-139	36.93544431446897	38.0	37.9	38.0	35.3	38.0
140-149	37.01021540075027	38.0	37.9	38.0	35.8	38.0
150-159	36.785311201645754	38.0	37.6	38.0	35.0	38.0
160-169	36.99380823686015	38.0	38.0	38.0	35.4	38.0
170-179	36.66375499173087	38.0	37.5	38.0	34.6	38.0
180-189	36.85788996006615	38.0	37.9	38.0	35.3	38.0
190-199	36.55845468113428	38.0	37.5	38.0	34.2	38.0
200-209	36.00189988302206	38.0	36.7	38.0	31.7	38.0
210-219	36.26127626961397	38.0	36.9	38.0	33.7	38.0
220-229	35.30797265136542	37.6	35.9	38.0	29.0	38.0
230-239	35.39801540881772	38.0	36.4	38.0	28.0	38.0
240-249	35.540829333225766	38.0	36.1	38.0	29.9	38.0
250-259	34.387481344036146	37.5	34.4	38.0	25.3	38.0
260-269	34.601024565366465	37.8	34.8	38.0	25.4	38.0
270-279	34.18851397684644	37.1	34.1	38.0	24.2	38.0
280-289	33.506722197571705	37.0	33.1	38.0	21.8	38.0
290-299	27.27624742850228	30.0	25.1	31.4	17.4	32.4
300-309	29.296464442741318	30.0	30.0	30.0	28.4	30.0
310-319	36.80682505748054	38.0	37.7	38.0	35.1	38.0
320-329	36.752137872615066	38.0	37.7	38.0	35.0	38.0
330-339	36.957046912185874	38.0	37.7	38.0	35.6	38.0
340-349	36.85132306078818	38.0	37.8	38.0	35.2	38.0
350-359	36.85038320358194	38.0	37.9	38.0	35.0	38.0
360-369	36.82031987414787	38.0	37.9	38.0	34.9	38.0
370-379	36.88349804364487	38.0	37.8	38.0	35.5	38.0
380-389	36.655102658222745	38.0	37.6	38.0	34.4	38.0
390-399	36.62997862127385	38.0	37.2	38.0	34.6	38.0
400-409	36.505889233996214	38.0	37.2	38.0	34.2	38.0
410-419	36.262657819369934	38.0	37.0	38.0	33.6	38.0
420-429	36.59504053890525	38.0	37.5	38.0	34.4	38.0
430-439	36.36126820217014	38.0	37.2	38.0	33.3	38.0
440-449	36.27542051550966	38.0	37.0	38.0	33.3	38.0
450-459	36.38179984671857	38.0	37.1	38.0	33.7	38.0
460-469	36.013464563752976	38.0	36.9	38.0	32.2	38.0
470-479	35.781805897301446	38.0	36.3	38.0	30.8	38.0
480-489	35.0257754830382	38.0	35.3	38.0	27.3	38.0
490-499	35.078306643539996	37.9	35.4	38.0	27.2	38.0
500-509	34.37884111169376	37.2	34.9	38.0	24.7	38.0
510-519	33.57124763018838	37.1	32.7	37.9	22.6	38.0
520-529	33.413212456133266	36.8	32.3	37.9	22.5	37.9
530-539	29.534556895647615	32.8	25.1	36.9	15.8	37.0
540-549	31.496226453148324	35.5	27.9	37.5	18.6	37.7
550-559	28.366832721552175	31.1	22.3	36.9	13.7	37.1
560-569	26.436660481626397	28.5	19.3	34.8	12.0	37.0
570-579	27.14339276350289	29.4	21.4	35.1	13.0	37.0
580-589	23.842571901093137	25.1	17.2	31.0	9.7	35.8
590-599	22.366679440119395	23.0	15.5	29.2	7.7	35.0
600-602	2.0	2.0	2.0	2.0	2.0	2.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	10.0
22	18.0
23	91.0
24	152.0
25	264.0
26	437.0
27	638.0
28	830.0
29	1137.0
30	1452.0
31	1942.0
32	2457.0
33	3584.0
34	5265.0
35	9569.0
36	19019.0
37	2716.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	0.02016860957605583	99.94352789318704	0.018151748618450245	0.018151748618450245
8	0.004033721915211166	0.004033721915211166	0.008067443830422332	99.98386511233916
9	0.002016860957605583	0.014118026703239077	99.93747731031422	0.0463878020249284
10-19	39.976604412891774	20.015126457182042	10.014118026703239	29.994151103222944
20-29	40.308176354322136	29.67044491952725	10.020571981767578	20.00080674438304
30-39	20.477592674761002	20.402162074946553	30.002218547053367	29.11802670323908
40-49	49.93949417127183	20.355169214634344	10.081884554878787	19.623452059215037
50-59	19.789439716025978	39.80557460368682	10.369690613529103	30.0352950667581
60-69	39.475414464926786	29.771086281311764	0.2706627405106692	30.482836513250778
70-79	49.88685410027833	10.17304667016256	19.943931265378563	19.99616796418055
80-89	39.23177766124803	30.49513936509217	29.448993586382155	0.8240893872776411
90-99	30.233149126699203	10.745835182122544	30.405994110766006	28.615021580412247
100-109	10.070388447420434	29.551450123028516	40.22044290266629	20.157718526884757
110-119	40.847484974385864	39.226533822758256	19.780767213908273	0.14521398894760196
120-129	20.710338429268685	49.42156427735872	20.042959138397	9.825138154975596
130-139	21.215965471340407	38.39982251623573	29.994756161510228	10.389455850913638
140-149	10.151062885724658	20.836190553023272	39.55326529789036	29.459481263361702
150-159	29.77512000322698	20.12282683231818	20.420918881852284	29.681134282602557
160-169	20.43140655883183	59.29450203702956	9.460691379936268	10.81340002420233
170-179	20.226695171634866	29.858416360776086	10.225485055060304	39.68940341252874
180-189	20.515307974668225	19.6896050986245	19.950183534347143	39.84490339236013
190-199	49.48832237505547	10.313016820620387	0.512282683231818	39.68637812109233
200-209	20.30636117946029	39.871727643096285	29.55588721713525	10.266023960308177
210-219	50.08289298535759	19.910249687386553	10.125448751563066	19.88140857569279
220-229	20.651042717115082	29.669839861239964	29.953410511879312	19.72570690976564
230-239	39.77471663103545	10.524585535073212	39.27715703279416	10.423540801097172
240-249	29.96026783913517	30.580452583598888	10.43745714170465	29.02182243556129
250-259	49.390907990803115	10.70489290468315	29.174700496147793	10.72949860836594
260-269	10.677060223468194	20.339841071356542	49.302166108668466	19.680932596506796
270-279	39.888669275140174	39.60106490258561	20.1155661328708	0.3946996894034125
280-289	20.221854705336614	10.262998668871768	10.933604937275625	58.58154168851599
290-299	38.047275220846274	26.559033520229114	12.346617724174095	23.047073534750513
300-309	45.54959461094752	14.70412649751926	24.072445645597192	15.673833245936025
310-319	34.001250453793716	25.629663990964463	15.396918236456777	24.972167318785043
320-329	0.10447339760396918	23.47989189625267	46.33899398975434	30.076640716389015
330-339	19.922955911419464	9.470775684724295	49.155540317050544	21.450728086805697
340-349	10.712758662417812	10.013109596224435	30.035093380662335	49.23903836069542
350-359	20.460449356621353	29.626073978459928	10.182727602759066	39.73074906215965
360-369	20.09781775644387	29.867895607276836	30.057278851196003	19.977007785083295
370-379	29.970553830018957	48.33972005969908	1.3105562502521075	20.37916986002985
380-389	19.988100520350127	0.12544875156306723	28.980880158121895	50.90557056996491
390-399	29.62123351216167	10.17304667016256	40.192005163164055	20.01371465451172
400-409	0.12202008793513776	20.142188697511195	30.70892662659836	49.0268645879553
410-419	19.429430035093382	10.666370860392885	39.92678794723892	29.97741115727482
420-429	9.923157597515226	20.695010285990882	19.344721874873947	50.03711024161994
430-439	20.273284659755557	38.85805332580372	29.897140091162118	10.971521923278608
440-449	0.05969908434512525	10.154289863256826	38.82659029486507	50.95942075753298
450-459	9.936468879835424	10.890040740591344	39.44717841152031	39.726311968052926
460-469	10.22064458876205	20.988261869226736	29.517768545036503	39.27332499697471
470-479	11.012464200718004	29.554878786656445	39.86265176878706	19.57000524383849
480-489	10.19523214069622	20.06232100359001	11.052398047678592	58.69004880803518
490-499	48.221330321487635	10.513896171997903	20.903957081198822	20.36081642531564
500-509	29.65269654310032	10.68270743414949	39.025856157476504	20.63873986527369
510-519	9.713807430115768	30.206324875963052	30.18071074180146	29.89915695211972
520-529	19.63111613085394	48.26912992618289	0.8152151990641765	31.28453874389899
530-539	29.263442378282438	9.72893388729781	11.659876568109395	49.347747166310356
540-549	9.969343713444395	20.60344479851559	29.197692711064498	40.22951877697552
550-559	19.91670364245089	29.221289984268484	30.14803759428825	20.713968778992374
560-569	19.966721794199508	28.75418498648703	29.70775684724295	21.57133637207051
570-579	10.047799604695253	20.29627687467226	19.971360574402	49.684562946230486
580-589	28.610382800209756	19.049655116776247	20.67585010689363	31.664111976120367
590-599	30.121213343552096	9.495986446694365	20.275503206808924	40.10729700294462
600-602	28.31672784478238	23.503153025963723	19.876837024188887	28.30328210506501
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.14285714285714285
46	0.2857142857142857
47	32.285714285714214
48	34.4285714285714
49	27.285714285714228
50	38.00000000000005
51	75.28571428571456
52	3983.1428571451866
53	4341.285714287751
54	248.99999999999534
55	26.28571428571423
56	12.142857142857137
57	3.5714285714285703
58	0.5714285714285714
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	0.0
350-359	0.0
360-369	0.0
370-379	0.0
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-479	0.0
480-489	0.0
490-499	0.0
500-509	0.0
510-519	0.0
520-529	0.0
530-539	0.0
540-549	0.0
550-559	0.0
560-569	0.0
570-579	0.0
580-589	0.0
590-599	0.0
600-602	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
602	49582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	0.5768222338751966
#Duplication Level	Percentage of total
1	0.2924448388528095
2	0.10084304788027913
3	0.06050582872816748
4	0.05647210681295631
5	0.09075874309225122
6	0.22992214916703643
7	0.11294421362591262
8	0.16134887660844663
9	0.1633657375660522
>10	0.9842281473115243
>50	0.35093380662337137
>100	2.391997095720221
>500	2.914364083740067
>1k	0.0
>5k	0.0
>10k+	92.0898713242709
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGCATGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGA	45660	92.0898713242709	No Hit
AGCATGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGG	780	1.5731515469323543	No Hit
AGCATGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGA	665	1.3412125368077126	No Hit
AGCATGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGA	341	0.6877495865435037	No Hit
AGCATGACTCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGA	313	0.6312774797305474	No Hit
AGCATGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGA	291	0.5869065386632246	No Hit
AGCATGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGC	125	0.25210761970069784	No Hit
AGCATGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC	116	0.23395587108224758	No Hit
AGCATGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGC	67	0.13512968415957405	No Hit
AGCATGACTCCTACGGGAGGCATCAGTAGGGAATCTTCCGCAATGGGCGA	54	0.10891049171070147	No Hit
AGCATGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGG	53	0.10689363075309588	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0	0.0
260-269	0.0	0.0	0.0	0.0	0.0	0.0
270-279	0.0	0.0	0.0	0.0	0.0	0.0
280-289	0.0	0.0	0.0	0.0	0.0	0.0
290-299	0.0	0.0	0.0	0.0	0.0	0.0
300-309	0.0	0.0	0.0	0.0	0.0	0.0
310-319	0.0	0.0	0.0	0.0	0.0	0.0
320-329	0.0	0.0	0.0	0.0	0.0	0.0
330-339	0.0	0.0	0.0	0.0	0.0	0.0
340-349	0.0	0.0	0.0	0.0	0.0	0.0
350-359	0.0	0.0	0.0	0.0	0.0	0.0
360-369	0.0	0.0	0.0	0.0	0.0	0.0
370-379	0.0	0.0	0.0	0.0	0.0	0.0
380-389	0.0	0.0	0.0	0.0	0.0	0.0
390-399	0.0	0.0	0.0	0.0	0.0	0.0
400-409	0.0	0.0	0.0	0.0	0.0	0.0
410-419	0.0	0.0	0.0	0.0	0.0	0.0
420-429	0.0	0.0	0.0	0.0	0.0	0.0
430-439	0.0	0.0	0.0	0.0	0.0	0.0
440-449	0.0	0.0	0.0	0.0	0.0	0.0
450-459	0.0	0.0	0.0	0.0	0.0	0.0
460-469	0.0	0.0	0.0	0.0	0.0	0.0
470-479	0.0	0.0	0.0	0.0	0.0	0.0
480-489	0.0	0.0	0.0	0.0	0.0	0.0
490-499	0.0	0.0	0.0	0.0	0.0	0.0
500-509	0.0	0.0	0.0	0.0	0.0	0.0
510-519	0.0	0.0	0.0	0.0	0.0	0.0
520-529	0.0	0.0	0.0	0.0	0.0	0.0
530-539	0.0	0.0	0.0	0.0	0.0	0.0
540-549	0.0	0.0	0.0	0.0	0.0	0.0
550-559	0.0	0.0	0.0	0.0	0.0	0.0
560-569	0.0	0.0	0.0	0.0	0.0	0.0
570-579	0.0	0.0	0.0	0.0	0.0	0.0
580-589	0.0	0.0	0.0	0.0	0.0	0.0
590-591	0.0	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
