FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR20982513

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20982513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37562
Total Bases22.6 Mbp
Sequences flagged as poor quality0
Sequence length602
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCCACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGC1313134.958202438634785No Hit
AGATCCACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGC921324.527447952718173No Hit
AGATCCACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGA407110.838081039348276No Hit
AGATCCACTCCTACGGGAGGCAGCAGTAGGGAATCTTTCACAATGGGCGA16964.515201533464672No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGACGA13833.6819125712155905No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGG11993.192055801075555No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGGGGAATTTTGCGCAATGGGGGA7702.049944092433843No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGGGGGATATTGGACAATGGGGGA5891.5680741174591344No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGA5041.3417815877748789No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGC4741.2619136361216123No Hit
AGATCCACTCCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGA3771.0036739257760503No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGC3510.9344550343432192No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGA3060.8146531068633193No Hit
AGATCCACTCCTACGGGAGGCAGCAGTTAGGAATATTCGTCAATGGGGGA2270.6043341675097172No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGG2040.5431020712422129No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGC1980.5271284809115596No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGG1760.46855864969916405No Hit
AGATCCACTCCTACGGGAGGCAGCAGTCGGGAATATTGCACAATGGAGGA1750.46589638464405514No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGG1540.40998881848676855No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGGGGGATATTGGACAATGGGGGG1520.4046642883765507No Hit
AGATCCACTCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGA1520.4046642883765507No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGT1350.35940578243969973No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGA1300.34609445716415527No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCTT850.2262925296842554No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGC740.19700761407805764No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGA680.1810340237474043No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGA650.17304722858207763No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGG610.1623981683616421No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGA530.141100047920771No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGAGA510.13577551781055322No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGA500.13311325275544433No Hit
AGATCCACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGCGA470.12512645759011767No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)