FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR20982517

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20982517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50425
Total Bases30.3 Mbp
Sequences flagged as poor quality0
Sequence length602
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC2332146.248884481903815No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGA1414228.045612295488347No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGA49829.88001983143282No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGA27275.408031730292514No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGA21094.182449181953396No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGC5941.1779871095686663No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGA5541.098661378284581No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGA1880.3728309370352008No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGC1710.33911750123946455No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGCGA900.17848289538919185No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGAGGG900.17848289538919185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)