##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR20982517
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	50425
Total Bases	30.3 Mbp
Sequences flagged as poor quality	0
Sequence length	602
%GC	54
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.63611303916708	38.0	38.0	38.0	37.0	38.0
2	37.42746653445712	38.0	38.0	38.0	37.0	38.0
3	37.44144769459594	38.0	38.0	38.0	37.0	38.0
4	37.19478433316807	38.0	38.0	38.0	36.0	38.0
5	37.49989092711949	38.0	38.0	38.0	37.0	38.0
6	37.520416460089244	38.0	38.0	38.0	37.0	38.0
7	37.51585523054041	38.0	38.0	38.0	37.0	38.0
8	37.66762518591968	38.0	38.0	38.0	37.0	38.0
9	37.36553296975706	38.0	38.0	38.0	37.0	38.0
10-19	37.50410708973723	38.0	38.0	38.0	36.8	38.0
20-29	37.366804164600886	38.0	38.0	38.0	36.5	38.0
30-39	37.17114923153198	38.0	38.0	38.0	36.2	38.0
40-49	37.28480118988597	38.0	38.0	38.0	36.6	38.0
50-59	37.20809122459097	38.0	38.0	38.0	36.3	38.0
60-69	37.279851264253836	38.0	38.0	38.0	36.5	38.0
70-79	37.169554784333165	38.0	38.0	38.0	36.0	38.0
80-89	36.960212196331184	38.0	37.7	38.0	35.7	38.0
90-99	37.03304313336639	38.0	38.0	38.0	35.8	38.0
100-109	37.00186018839861	38.0	37.8	38.0	35.9	38.0
110-119	37.02890629647992	38.0	37.7	38.0	35.7	38.0
120-129	36.97396926127912	38.0	37.9	38.0	35.3	38.0
130-139	36.83765989092713	38.0	37.5	38.0	35.1	38.0
140-149	36.72314129895885	38.0	37.6	38.0	34.5	38.0
150-159	36.68789092711948	38.0	37.4	38.0	34.3	38.0
160-169	36.81212890431334	38.0	37.5	38.0	34.9	38.0
170-179	36.62847595438771	38.0	37.4	38.0	34.4	38.0
180-189	36.67765195835399	38.0	37.5	38.0	34.3	38.0
190-199	36.60556470004958	38.0	37.6	38.0	34.0	38.0
200-209	36.01360436291522	38.0	36.5	38.0	32.2	38.0
210-219	36.11160337134358	38.0	36.9	38.0	33.2	38.0
220-229	35.713481408031726	38.0	36.2	38.0	30.5	38.0
230-239	35.56553098661378	38.0	36.3	38.0	29.4	38.0
240-249	35.182968765493314	38.0	35.8	38.0	26.8	38.0
250-259	35.022127912741695	38.0	35.2	38.0	26.2	38.0
260-269	34.81754685176004	37.9	35.0	38.0	25.2	38.0
270-279	33.84819633118493	37.1	34.0	38.0	22.7	38.0
280-289	33.32103916707982	37.0	33.1	38.0	21.1	38.0
290-299	23.152115022310362	26.2	21.0	26.7	14.4	27.2
300-309	30.05066137828458	30.8	30.8	30.8	29.1	30.8
310-319	36.77641447694596	38.0	37.7	38.0	35.2	38.0
320-329	36.76087059990084	38.0	37.4	38.0	34.7	38.0
330-339	36.951371343579574	38.0	37.7	38.0	35.5	38.0
340-349	36.67821318790283	38.0	37.4	38.0	34.4	38.0
350-359	36.70279424888448	38.0	37.6	38.0	34.5	38.0
360-369	36.60896975706495	38.0	37.3	38.0	33.9	38.0
370-379	36.59323946455132	38.0	37.1	38.0	34.4	38.0
380-389	36.51812592959842	38.0	37.4	38.0	34.2	38.0
390-399	36.12080317302925	38.0	36.6	38.0	32.6	38.0
400-409	35.94523549826474	38.0	36.8	38.0	31.3	38.0
410-419	36.26848785324739	38.0	37.0	38.0	33.2	38.0
420-429	36.36844422409519	38.0	37.1	38.0	33.5	38.0
430-439	35.95467327714427	38.0	36.7	38.0	31.6	38.0
440-449	35.6269905800694	38.0	36.0	38.0	30.4	38.0
450-459	35.384606841844324	37.9	35.8	38.0	29.0	38.0
460-469	35.49908973723352	38.0	36.2	38.0	29.8	38.0
470-479	34.88562221120476	37.9	35.0	38.0	26.4	38.0
480-489	34.61187902825979	37.8	34.6	38.0	25.0	38.0
490-499	34.36144769459594	37.7	34.4	38.0	24.7	38.0
500-509	33.418562221120474	36.8	33.1	38.0	21.6	38.0
510-519	32.48533465542885	36.3	29.9	37.8	20.0	38.0
520-529	31.55178780366881	36.1	27.8	37.7	18.4	37.7
530-539	28.8318671294001	32.1	23.2	36.9	14.3	37.0
540-549	30.29361626177491	34.4	25.0	37.3	16.3	37.7
550-559	27.145893901834405	29.2	20.4	36.5	11.7	37.1
560-569	25.038544372830938	26.4	18.2	33.1	9.6	37.0
570-579	24.257901834407537	25.2	17.0	32.7	8.3	37.0
580-589	22.606044620723846	22.9	15.7	30.1	8.7	35.2
590-599	17.032541398116017	17.6	11.5	22.4	6.2	27.5
600-602	2.0	2.0	2.0	2.0	2.0	2.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	12.0
22	33.0
23	105.0
24	236.0
25	419.0
26	543.0
27	816.0
28	1121.0
29	1430.0
30	1769.0
31	2369.0
32	2960.0
33	4317.0
34	6557.0
35	11559.0
36	15688.0
37	491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	0.00991571641051066	99.97421913733268	0.005949429846306396	0.00991571641051066
8	0.0	0.003966286564204264	0.00991571641051066	99.98611799702527
9	0.003966286564204264	0.015865146256817057	99.94248884481904	0.03767972235994051
10-19	39.974020823004466	20.01189885969261	10.013683688646505	30.00039662865642
20-29	44.90748636588994	22.21259295984135	10.00951908775409	22.870401586514628
30-39	25.86276648487853	32.25463559742192	30.043629152206247	11.838968765493307
40-49	42.06385721368369	29.342786316311354	11.645413981160138	16.94794248884482
50-59	18.571343579573625	35.08993554784333	19.341398116013885	26.997322756569165
60-69	34.19593455627169	19.4058502726822	9.22875557759048	37.16945959345563
70-79	51.07942488844819	9.87922657411998	20.164204263758055	18.877144273673775
80-89	28.81130391670798	37.49826474962816	16.079127416955874	17.611303916707982
90-99	35.88874566187407	10.841249380267724	44.09201784828954	9.177987109568667
100-109	8.443827466534456	29.73049082796232	35.79593455627169	26.02974714923153
110-119	55.401883986117994	24.86524541398116	11.779474467030242	7.9533961328705995
120-129	24.748041646008925	43.73644025780863	24.99553792761527	6.519980168567178
130-139	22.952702032721863	36.475557759048094	25.260684184432325	15.31105602379772
140-149	18.703222607833418	35.14149727317798	26.54734754586019	19.60793257312841
150-159	24.830540406544372	26.080912245909765	19.54268715914725	29.54586018839861
160-169	30.340307387208725	36.78532473971244	12.612196331184927	20.262171541893903
170-179	26.54635597421914	24.91343579573624	8.922359940505702	39.61784828953892
180-189	22.238770451165095	23.780466038671292	18.41864154685176	35.562121963311846
190-199	40.18800198314329	16.846207238472978	9.474268715914725	33.491522062469016
200-209	34.01011403073872	27.97223599405057	25.017947446703026	12.999702528507683
210-219	40.68299454635597	20.151512146752605	16.54020823004462	22.6252850768468
220-229	27.573822508676255	31.529201784828953	29.897669806643528	10.999305899851263
230-239	39.479623202776395	13.408031730292514	30.78235002478929	16.329995042141796
240-249	27.882994546355977	30.30441249380268	19.531978185423895	22.28061477441745
250-259	42.94119980168567	18.252850768468022	22.94695091720377	15.858998512642538
260-269	37.02032721864155	14.950917203767972	26.275855230540408	21.752900347050076
270-279	30.523748140803175	32.159643034209225	21.04987605354487	16.266732771442737
280-289	29.43024293505206	12.88527516113039	11.391769955379276	46.292711948438274
290-299	22.178681209717404	30.096975706494796	22.276053544868617	25.448289538919187
300-309	34.82875557759048	25.131779871095688	14.821021318790283	25.21844323252355
310-319	33.65294992563213	25.84154685176004	15.298760535448686	25.206742687159146
320-329	0.06187407040158652	23.261874070401586	46.475359444719885	30.20089241447695
330-339	16.95686663361428	2.8487853247397124	46.04481903817551	34.149529003470505
340-349	12.30738720872583	8.211998016856718	30.08487853247397	49.39573624194348
350-359	29.79652949925632	16.829747149231533	15.547050074367874	37.82667327714427
360-369	21.36856717897868	19.355676747645017	42.373029251363405	16.90272682201289
370-379	44.853941497273176	20.21259295984135	12.338919186911253	22.594546355974217
380-389	19.965493306891425	0.1965294992563213	21.745562716906296	58.09241447694596
390-399	25.259890927119482	20.723252354982648	30.78155676747645	23.235299950421417
400-409	11.292612791274168	20.55825483391175	27.190282597917697	40.958849776896386
410-419	10.067228557263261	21.138125929598413	40.39841348537432	28.396232027764007
420-429	9.928408527516112	20.51760039662866	22.496777392166585	47.057213683688644
430-439	18.34348041646009	34.37223599405057	25.325334655428854	21.958948934060484
440-449	7.702528507684681	7.148041646008924	19.83083787803669	65.31859196826971
450-459	6.784928111056024	29.4913237481408	29.823698562221118	33.900049578582056
460-469	18.764700049578583	15.698363906792265	21.308477937530988	44.22845810609817
470-479	18.635994050570154	29.885176003966286	39.109766980664354	12.369062964799207
480-489	23.83163113534953	15.175210708973724	20.211601388200297	40.78155676747645
490-499	41.13475458601884	10.737134357957363	20.211998016856718	27.91611303916708
500-509	26.555081804660386	19.484184432325236	30.326623698562223	23.63411006445216
510-519	10.684184432325235	24.282994546355972	27.63648983639068	37.39633118492811
520-529	20.403966286564206	39.687059990084286	2.7305899851264255	37.17838373822509
530-539	27.596628656420425	8.41011403073872	17.419137332672285	46.57411998016857
540-549	8.730986613782846	23.149628160634606	29.266435299950423	38.852949925632124
550-559	22.524739712444227	28.397620228061477	27.905800694100147	21.17183936539415
560-569	19.902231036192365	24.701636093207735	28.895587506197323	26.500545364402576
570-579	13.974814080317302	14.135448686167576	24.487258304412492	47.40247892910263
580-589	28.61616261774913	18.785919682697074	20.846802181457612	31.751115518096185
590-599	35.49033217649975	5.021318790282598	24.209816559246406	35.27853247397125
600-602	30.478929102627667	23.000495785820526	21.25731284085275	25.26326227069906
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.14285714285714285
45	1.571428571428571
46	81.85714285714295
47	391.42857142858963
48	654.2857142857207
49	98.9999999999996
50	71.57142857142895
51	46.571428571428804
52	42.42857142857157
53	267.14285714285415
54	483.71428571431966
55	2022.8571428568382
56	3097.8571428582645
57	1118.4285714284724
58	27.285714285714228
59	1.7142857142857137
60	0.2857142857142857
61	0.14285714285714285
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	0.0
350-359	0.0
360-369	0.0
370-379	0.0
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-479	0.0
480-489	0.0
490-499	0.0
500-509	0.0
510-519	0.0
520-529	0.0
530-539	0.0
540-549	0.0
550-559	0.0
560-569	0.0
570-579	0.0
580-589	0.0
590-599	0.0
600-602	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
602	50425.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.0193356470004957
#Duplication Level	Percentage of total
1	0.5493306891422906
2	0.30143777887952405
3	0.24987605354486864
4	0.23797719385225585
5	0.20823004462072384
6	0.10708973723351511
7	0.12493802677243432
8	0.06346058502726823
9	0.16063460585027267
>10	0.886465047099653
>50	0.3569657907783837
>100	0.7119484382746654
>500	2.2766484878532474
>1k	19.47050074367873
>5k	0.0
>10k+	74.29449677739217
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC	23321	46.248884481903815	No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGA	14142	28.045612295488347	No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGA	4982	9.88001983143282	No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGA	2727	5.408031730292514	No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGA	2109	4.182449181953396	No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGC	594	1.1779871095686663	No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGA	554	1.098661378284581	No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGA	188	0.3728309370352008	No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGC	171	0.33911750123946455	No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGCGA	90	0.17848289538919185	No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGAGGG	90	0.17848289538919185	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0	0.0
260-269	0.0	0.0	0.0	0.0	0.0	0.0
270-279	0.0	0.0	0.0	0.0	0.0	0.0
280-289	0.0	0.0	0.0	0.0	0.0	0.0
290-299	0.0	0.0	0.0	0.0	0.0	0.0
300-309	0.0	0.0	0.0	0.0	0.0	0.0
310-319	0.0	0.0	0.0	0.0	0.0	0.0
320-329	0.0	0.0	0.0	0.0	0.0	0.0
330-339	0.0	0.0	0.0	0.0	0.0	0.0
340-349	0.0	0.0	0.0	0.0	0.0	0.0
350-359	0.0	0.0	0.0	0.0	0.0	0.0
360-369	0.0	0.0	0.0	0.0	0.0	0.0
370-379	0.0	0.0	0.0	0.0	0.0	0.0
380-389	0.0	0.0	0.0	0.0	0.0	0.0
390-399	0.0	0.0	0.0	0.0	0.0	0.0
400-409	0.0	0.0	0.0	0.0	0.0	0.0
410-419	0.0	0.0	0.0	0.0	0.0	0.0
420-429	0.0	0.0	0.0	0.0	0.0	0.0
430-439	0.0	0.0	0.0	0.0	0.0	0.0
440-449	0.0	0.0	0.0	0.0	0.0	0.0
450-459	0.0	0.0	0.0	0.0	0.0	0.0
460-469	0.0	0.0	0.0	0.0	0.0	0.0
470-479	0.0	0.0	0.0	0.0	0.0	0.0
480-489	0.0	0.0	0.0	0.0	0.0	0.0
490-499	0.0	0.0	0.0	0.0	0.0	0.0
500-509	0.0	0.0	0.0	0.0	0.0	0.0
510-519	0.0	0.0	0.0	0.0	0.0	0.0
520-529	0.0	0.0	0.0	0.0	0.0	0.0
530-539	0.0	0.0	0.0	0.0	0.0	0.0
540-549	0.0	0.0	0.0	0.0	0.0	0.0
550-559	0.0	0.0	0.0	0.0	0.0	0.0
560-569	0.0	0.0	0.0	0.0	0.0	0.0
570-579	0.0	0.0	0.0	0.0	0.0	0.0
580-589	0.0	0.0	0.0	0.0	0.0	0.0
590-591	0.0	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
