##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR20982519
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41491
Total Bases	24.9 Mbp
Sequences flagged as poor quality	0
Sequence length	602
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.752090814875515	34.0	34.0	34.0	34.0	34.0
2	33.851534067629125	34.0	34.0	34.0	34.0	34.0
3	33.80219806705069	34.0	34.0	34.0	34.0	34.0
4	37.499722831457426	38.0	38.0	38.0	37.0	38.0
5	37.598563543901086	38.0	38.0	38.0	37.0	38.0
6	37.612976308115016	38.0	38.0	38.0	37.0	38.0
7	37.50263912655757	38.0	38.0	38.0	37.0	38.0
8	37.66602395700272	38.0	38.0	38.0	37.0	38.0
9	37.51273770215227	38.0	38.0	38.0	37.0	38.0
10-19	37.453970740642546	38.0	38.0	38.0	36.9	38.0
20-29	37.46755922971247	38.0	38.0	38.0	36.7	38.0
30-39	37.39948422549469	38.0	38.0	38.0	36.7	38.0
40-49	37.24422404858885	38.0	37.9	38.0	36.5	38.0
50-59	37.065561206044684	38.0	37.9	38.0	35.6	38.0
60-69	37.32488009447832	38.0	38.0	38.0	36.6	38.0
70-79	37.11121207008749	38.0	37.7	38.0	35.8	38.0
80-89	37.199276951628065	38.0	38.0	38.0	36.7	38.0
90-99	36.985688462558144	38.0	38.0	38.0	35.5	38.0
100-109	36.62305078209732	38.0	37.3	38.0	34.6	38.0
110-119	36.63206960545661	38.0	37.2	38.0	34.5	38.0
120-129	36.68153334458076	38.0	37.3	38.0	34.7	38.0
130-139	36.77897134318286	38.0	37.4	38.0	34.9	38.0
140-149	36.433585596876426	38.0	37.0	38.0	33.7	38.0
150-159	36.52776505748235	38.0	37.0	38.0	34.1	38.0
160-169	36.64667759273095	38.0	37.1	38.0	34.2	38.0
170-179	36.52400279578704	38.0	37.1	38.0	34.0	38.0
180-189	36.39731749054012	38.0	37.1	38.0	33.4	38.0
190-199	36.225701959461084	38.0	36.9	38.0	33.5	38.0
200-209	35.756159167048274	38.0	36.7	38.0	29.9	38.0
210-219	35.88401340049649	38.0	36.8	38.0	31.4	38.0
220-229	34.69094020389964	37.6	35.2	38.0	25.9	38.0
230-239	34.9821720373093	37.8	35.6	38.0	26.8	38.0
240-249	34.80242944252971	38.0	35.1	38.0	25.6	38.0
250-259	34.014979152105276	37.4	33.0	38.0	23.2	38.0
260-269	34.151428020534574	37.3	34.1	38.0	24.1	38.0
270-279	33.858885059410476	37.2	33.5	38.0	23.3	38.0
280-289	33.41646622159023	36.8	32.7	38.0	21.5	38.0
290-299	31.734458075245232	35.6	29.0	37.5	18.9	37.9
300-309	28.97026343062351	29.6	29.6	29.6	28.6	29.6
310-319	36.90654358776602	38.0	37.9	38.0	35.2	38.0
320-329	36.84837675640501	38.0	37.7	38.0	35.2	38.0
330-339	36.944506037453905	38.0	37.9	38.0	35.3	38.0
340-349	36.83056566484297	38.0	37.5	38.0	35.3	38.0
350-359	36.5961292810489	38.0	37.6	38.0	34.4	38.0
360-369	36.6427128774915	38.0	37.4	38.0	34.2	38.0
370-379	36.543725145212214	38.0	37.0	38.0	34.2	38.0
380-389	36.59517726735918	38.0	37.4	38.0	34.4	38.0
390-399	36.51544913354703	38.0	37.1	38.0	34.0	38.0
400-409	36.748032103347725	38.0	37.3	38.0	34.9	38.0
410-419	36.542153720083874	38.0	37.2	38.0	34.2	38.0
420-429	36.462999204646785	38.0	37.1	38.0	34.0	38.0
430-439	36.182128654406974	38.0	37.2	38.0	32.7	38.0
440-449	36.059383962787116	38.0	37.0	38.0	31.8	38.0
450-459	36.3649201031549	38.0	37.0	38.0	33.7	38.0
460-469	35.68062953411583	37.8	36.2	38.0	30.3	38.0
470-479	35.97741919934443	38.0	36.5	38.0	31.9	38.0
480-489	35.49427827721674	38.0	36.1	38.0	29.2	38.0
490-499	35.35164975536863	37.9	35.7	38.0	28.6	38.0
500-509	34.864546528162734	38.0	35.3	38.0	26.1	38.0
510-519	34.34869248752742	37.2	34.4	37.9	24.5	38.0
520-529	33.97206623123087	36.9	33.6	38.0	23.7	38.0
530-539	31.338555349352873	35.5	28.4	37.0	18.6	37.0
540-549	32.33377840977561	36.1	30.3	37.4	20.4	37.7
550-559	29.6549854185245	33.3	24.5	36.8	16.1	37.2
560-569	27.39881661083126	30.0	20.9	35.8	13.7	37.0
570-579	28.109189944807305	29.9	22.9	36.6	14.5	37.0
580-589	25.02431611674821	26.7	18.7	32.1	10.8	36.2
590-599	23.49796341375238	24.9	16.4	30.7	8.0	36.6
600-602	4.774175925702763	3.6666666666666665	3.6666666666666665	5.666666666666667	3.6666666666666665	7.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	4.0
22	30.0
23	73.0
24	167.0
25	260.0
26	348.0
27	509.0
28	763.0
29	915.0
30	1237.0
31	1532.0
32	2081.0
33	2733.0
34	4038.0
35	6675.0
36	14523.0
37	5602.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	0.014460967438721651	99.97107806512255	0.01205080619893471	0.0024101612397869417
8	0.004820322479573883	0.004820322479573883	0.019281289918295533	99.97107806512255
9	0.004820322479573883	0.0024101612397869417	99.95661709768383	0.036152418596804126
10-19	39.96746282326288	20.015184015810657	10.024824660769806	29.992528500156663
20-29	40.25427201079752	29.71535995758116	10.018076209298401	20.012291822322915
30-39	20.687619001711212	20.629052083584394	30.011327757827	28.672001156877396
40-49	33.11995372490419	37.03477862669013	10.403219975416356	19.442047672989325
50-59	19.41674097997156	30.985032898700922	27.095755706056735	22.502470415270782
60-69	37.590079776337035	31.69554843219011	0.04217782169627148	30.67219396977658
70-79	50.43985442626112	10.013978935190766	19.536284977464994	20.009881661083124
80-89	39.25068087055024	39.55002289653178	20.958762141187247	0.24053409173073678
90-99	30.00530235472753	10.93972186739293	30.652671663734303	28.402304114145238
100-109	19.126557566701212	20.093273239979755	40.10050372369911	20.679665469619916
110-119	49.85201609987708	30.45961774842737	19.34057988479429	0.3477862669012557
120-129	32.08430744016775	51.12434021836061	12.246752307729388	4.544600033742258
130-139	28.77973536429587	31.92138054035815	28.098864814056064	11.200019281289917
140-149	12.91026969704273	13.766599985539033	50.77317972572365	22.549950591694586
150-159	29.339856836422356	27.695644838639705	27.463305295124247	15.501193029813695
160-169	20.805957918584753	49.91684943722735	8.175748957605263	21.101443686582634
170-179	20.65700995396592	29.79369019787424	19.958786242799643	29.590513605360197
180-189	22.222409679207537	19.613651153262154	19.613651153262154	38.550288014268155
190-199	48.22226506953315	11.07613699356487	2.002843990262949	38.69875394663903
200-209	20.108698271914392	39.315032175652554	29.518449784290567	11.057819768142489
210-219	49.7013810223904	20.588320358631993	10.396471523944951	19.313827095032657
220-229	29.502301703983996	12.575498300836326	38.14513990985997	19.777060085319707
230-239	39.818996890892	10.624231761104816	38.838061266298716	10.718710081704467
240-249	29.995661709768385	39.38155262587067	18.80166783157793	11.821117832783013
250-259	47.80120990094237	11.806174833096335	20.469499409510497	19.923115856450796
260-269	13.846858354823938	34.20910558916391	31.84401436456099	20.100021691451158
270-279	38.94242124798149	39.50350678460389	19.86888722855559	1.6851847388590295
280-289	19.476512978718276	2.8661637463546312	32.15179195488178	45.505531320045314
290-299	27.719987467161555	49.985298016437305	2.655033621749295	19.63968089465185
300-309	34.46096743872165	15.120869586175315	25.171723988334822	25.246438986768215
310-319	34.127883155383095	26.049504711865225	14.900821864982767	24.921790267768916
320-329	0.14195849702345087	23.832156371261238	45.98153816490323	30.04434696681208
330-339	19.8631028415801	9.379383480754862	39.851534067629125	30.90597961003591
340-349	10.834397821214239	10.064110288978332	39.27839772480779	39.82309416499964
350-359	30.03928562820853	19.9185365500952	10.585910197392206	39.45626762430406
360-369	29.35335373936516	20.898749126316552	30.144127642139257	19.603769492179026
370-379	36.69856113373985	38.730327058880235	1.6401147236750138	22.9309970837049
380-389	19.98674411318117	0.19257188305897666	20.519389747174085	59.30129425658577
390-399	29.61220505651828	11.06312212287002	40.01590706418259	19.308765756429107
400-409	0.44684389385649903	29.296232917982213	40.54831168205153	29.70861150610976
410-419	10.388517991853655	20.02988599937336	39.222964016292686	30.358631992480294
420-429	9.600154250319347	20.831505627726496	11.094936251235207	58.473403870718954
430-439	28.869152346291965	30.138102239039792	29.610999975898388	11.381745438769855
440-449	2.0604468438938564	10.526379214769468	29.621363669229474	57.7918102721072
450-459	9.56617097683835	10.643513051023113	38.89060278132607	40.89971319081246
460-469	12.251090597961003	36.355836205442145	18.642597189751996	32.750476006844856
470-479	21.848352654792606	18.56595406232677	47.556819551227974	12.028873731652649
480-489	11.044081849075702	17.828444722951968	32.09009182714323	39.03738160082909
490-499	45.52577667445952	13.977971126268349	20.580607842664676	19.915644356607455
500-509	19.734400231375478	29.14728495336338	30.494083054156324	20.62423176110482
510-519	9.856836422356656	30.22077076956448	28.827215540719674	31.095177267359187
520-529	19.5791858475332	30.842592369429518	16.351015882962567	33.227205900074715
530-539	21.53551372586826	8.324214889976139	20.936829673905184	49.20344171025042
540-549	9.910100985755948	20.95273673808778	29.11980911522981	40.01735316092647
550-559	20.2511388011858	38.34205008315056	20.83801306307392	20.568798052589717
560-569	20.197874237786507	28.626690125569397	22.310380564459763	28.865055072184333
570-579	10.008676580463232	20.23474970475525	20.139307319659686	49.61726639512183
580-589	28.910607119616305	19.00894169819961	20.54758863367959	31.532862548504493
590-599	30.482755296329323	9.946976452724687	20.223903979176207	39.34636427176978
600-602	45.69987065468013	15.734335960409085	15.412177741357564	23.15361564355322
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.14285714285714285
43	0.0
44	0.14285714285714285
45	0.2857142857142857
46	0.5714285714285714
47	33.285714285714235
48	56.285714285714725
49	60.28571428571481
50	214.14285714285347
51	1607.2857142855041
52	3730.1428571448414
53	548.7142857143161
54	874.1428571428136
55	44.71428571428591
56	9.000000000000005
57	2.7142857142857135
58	5.571428571428574
59	2.999999999999999
60	0.2857142857142857
61	0.0
62	0.0
63	0.0
64	0.0
65	0.14285714285714285
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	0.0
350-359	0.0
360-369	0.0
370-379	0.0
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-479	0.0
480-489	0.0
490-499	0.0
500-509	0.0
510-519	0.0
520-529	0.0
530-539	0.0
540-549	0.0
550-559	0.0
560-569	0.0
570-579	0.0
580-589	0.0
590-599	0.0
600-602	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
602	41491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	0.9038104649201031
#Duplication Level	Percentage of total
1	0.4675712805186667
2	0.22173483406039865
3	0.1735316092646598
4	0.2602974138969897
5	0.18076209298402063
6	0.18799257670338146
7	0.13496902942806874
8	0.07712515967318213
9	0.1735316092646598
>10	1.289436263286014
>50	0.1277385457087079
>100	0.829095466486708
>500	1.9160781856306188
>1k	3.542937022486804
>5k	18.11959220071823
>10k+	72.2976067098889
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTCTGTACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGC	29997	72.2976067098889	No Hit
GTCTGTACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGC	7518	18.11959220071823	No Hit
GTCTGTACTCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGA	1470	3.542937022486804	No Hit
GTCTGTACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGA	795	1.9160781856306188	No Hit
GTCTGTACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGCCGC	232	0.5591574076305705	No Hit
GTCTGTACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGAGGA	112	0.2699380588561375	No Hit
GTCTGTACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGG	53	0.1277385457087079	No Hit
GTCTGTACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGA	45	0.10845725579041238	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0	0.0
260-269	2.4101612397869416E-4	0.0	0.0	0.0	0.0	0.0
270-279	0.00698946759538213	0.0	0.0	0.0	0.0	0.0
280-289	0.010122677207105156	0.0	0.0	0.0	0.0	0.0
290-299	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
300-309	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
310-319	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
320-329	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
330-339	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
340-349	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
350-359	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
360-369	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
370-379	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
380-389	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
390-399	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
400-409	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
410-419	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
420-429	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
430-439	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
440-449	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
450-459	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
460-469	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
470-479	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
480-489	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
490-499	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
500-509	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
510-519	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
520-529	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
530-539	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
540-549	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
550-559	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
560-569	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
570-579	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
580-589	0.01205080619893471	0.0	0.0	0.0	0.0	0.0
590-591	0.012050806198934708	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
