FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR20982521

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20982521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45915
Total Bases27.6 Mbp
Sequences flagged as poor quality0
Sequence length602
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCACACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGA2650457.72405531961232No Hit
ACTCACACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC854118.601764129369485No Hit
ACTCACACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGC662414.426657954916694No Hit
ACTCACACTCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGA13002.831318741152129No Hit
ACTCACACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGC8641.8817379941195687No Hit
ACTCACACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGA4130.8994881846890994No Hit
ACTCACACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGC2520.5488402482848742No Hit
ACTCACACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGG660.1437438745508004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)