##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR20982521
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	45915
Total Bases	27.6 Mbp
Sequences flagged as poor quality	0
Sequence length	602
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.84595448110639	34.0	34.0	34.0	34.0	34.0
2	33.86684090166612	34.0	34.0	34.0	34.0	34.0
3	37.53668735707285	38.0	38.0	38.0	37.0	38.0
4	37.534836110203635	38.0	38.0	38.0	37.0	38.0
5	37.42999019928128	38.0	38.0	38.0	37.0	38.0
6	37.45660459544811	38.0	38.0	38.0	37.0	38.0
7	37.57946204943918	38.0	38.0	38.0	37.0	38.0
8	37.7244473483611	38.0	38.0	38.0	37.0	38.0
9	37.40446477186105	38.0	38.0	38.0	37.0	38.0
10-19	37.44546662310792	38.0	38.0	38.0	36.8	38.0
20-29	37.37258847871066	38.0	38.0	38.0	36.6	38.0
30-39	37.3618556027442	38.0	38.0	38.0	36.9	38.0
40-49	37.35299139714691	38.0	38.0	38.0	36.9	38.0
50-59	37.19935315256452	38.0	38.0	38.0	36.0	38.0
60-69	37.3019536099314	38.0	38.0	38.0	36.8	38.0
70-79	37.223926821300225	38.0	38.0	38.0	36.3	38.0
80-89	37.26921049765872	38.0	38.0	38.0	36.8	38.0
90-99	37.089476206032884	38.0	38.0	38.0	35.9	38.0
100-109	37.1298856582816	38.0	37.9	38.0	36.5	38.0
110-119	37.05802461069367	38.0	38.0	38.0	36.0	38.0
120-129	36.80717412610258	38.0	37.5	38.0	34.9	38.0
130-139	36.89793095938147	38.0	37.6	38.0	35.3	38.0
140-149	36.829079821409124	38.0	37.8	38.0	34.9	38.0
150-159	36.743676358488514	38.0	37.6	38.0	34.4	38.0
160-169	36.86956550147011	38.0	38.0	38.0	35.0	38.0
170-179	36.688411194598714	38.0	37.5	38.0	34.4	38.0
180-189	36.738573450941956	38.0	37.6	38.0	34.7	38.0
190-199	36.52395949036262	38.0	37.5	38.0	34.2	38.0
200-209	35.96599368398127	38.0	36.6	38.0	31.7	38.0
210-219	36.180808014809976	38.0	36.9	38.0	33.3	38.0
220-229	35.42955243384515	37.8	35.9	38.0	29.7	38.0
230-239	35.361971033431345	38.0	36.2	38.0	27.9	38.0
240-249	35.15364042251988	38.0	35.8	38.0	27.0	38.0
250-259	34.45041489709245	37.6	34.4	38.0	24.7	38.0
260-269	34.65565937057606	37.8	34.7	38.0	25.2	38.0
270-279	34.18319067842753	37.3	34.1	38.0	23.7	38.0
280-289	33.609648263094854	36.9	33.7	38.0	21.7	38.0
290-299	28.047097898290314	30.9	25.6	32.7	17.2	33.9
300-309	29.2907829685288	30.0	29.9	30.0	28.6	30.0
310-319	36.79915713819013	38.0	37.8	38.0	34.9	38.0
320-329	36.746784275291304	38.0	37.6	38.0	34.8	38.0
330-339	37.005584231732556	38.0	38.0	38.0	35.7	38.0
340-349	36.82259392355439	38.0	37.6	38.0	35.1	38.0
350-359	36.631281716214744	38.0	37.3	38.0	34.3	38.0
360-369	36.51032342371774	38.0	37.2	38.0	34.1	38.0
370-379	36.525190025046285	38.0	37.0	38.0	34.1	38.0
380-389	36.46308395949036	38.0	37.4	38.0	33.7	38.0
390-399	36.4548165087662	38.0	37.1	38.0	34.0	38.0
400-409	36.47189153871284	38.0	37.0	38.0	33.9	38.0
410-419	36.26140694762061	38.0	36.9	38.0	33.3	38.0
420-429	36.44722421866492	38.0	37.1	38.0	33.7	38.0
430-439	36.092342371773924	38.0	37.1	38.0	31.9	38.0
440-449	35.93249047152347	38.0	36.7	38.0	31.5	38.0
450-459	35.96767287378852	38.0	36.9	38.0	31.8	38.0
460-469	35.59311771752151	38.0	36.5	38.0	29.8	38.0
470-479	35.614542088642054	38.0	36.4	38.0	30.1	38.0
480-489	35.328330610911465	38.0	36.1	38.0	28.3	38.0
490-499	35.22234128280518	38.0	35.4	38.0	28.0	38.0
500-509	34.41754328650768	37.6	34.7	38.0	24.3	38.0
510-519	33.894494174017204	37.2	33.4	37.9	23.5	38.0
520-529	33.15353370358271	36.6	32.0	37.8	21.8	37.9
530-539	30.439610149188717	34.1	26.4	37.0	17.4	37.0
540-549	32.05198954590003	36.0	29.2	37.5	19.8	37.8
550-559	29.04850920178591	31.9	23.3	36.9	14.9	37.1
560-569	26.782789937928783	28.9	20.2	35.0	13.0	37.0
570-579	27.34393335511271	29.6	21.5	35.1	13.9	37.0
580-589	24.067746923663293	25.6	17.2	31.0	9.8	36.2
590-599	22.589959708156375	23.3	15.5	29.6	7.7	35.4
600-602	2.0	2.0	2.0	2.0	2.0	2.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	8.0
22	22.0
23	78.0
24	113.0
25	250.0
26	421.0
27	656.0
28	846.0
29	1092.0
30	1410.0
31	1808.0
32	2304.0
33	3184.0
34	4724.0
35	8233.0
36	17261.0
37	3503.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.013067624959163672	99.96079712512251	0.013067624959163672	0.013067624959163672
8	0.0	0.006533812479581836	0.006533812479581836	99.98693237504084
9	0.0	0.006533812479581836	99.9281280627246	0.06533812479581835
10-19	39.969291081345965	20.020908199934663	10.016116737449634	29.993683981269736
20-29	42.012414243711206	27.967113143852774	10.020254818686704	20.00021779374932
30-39	22.38897963628444	22.334748992703908	30.015245562452357	25.261025808559296
40-49	39.041271915496026	31.0948491778286	10.32385930523794	19.54001960143744
50-59	19.561798976369378	36.6052488293586	21.08918654034629	22.74376565392573
60-69	37.395404551889364	28.248067080474794	1.9605793313731894	32.395949036262664
70-79	54.26244146792987	4.115866274637918	21.627790482413154	19.993901775019058
80-89	31.665033213546774	39.57159969508875	18.866601328541872	9.896765762822607
90-99	31.922465425242297	16.483284329739735	32.328215180224326	19.26603506479364
100-109	11.488402482848741	27.886311662855277	34.34411412392465	26.281171730371337
110-119	47.58945878253294	34.327997386475005	16.012196449961884	2.0703473810301647
120-129	38.0516171185887	34.753784166394425	20.084503974735927	7.110094740280953
130-139	26.789937928781445	38.3110094740281	23.69835565719264	11.20069693999782
140-149	12.093869105956658	14.058804312316237	46.471741261025805	27.375585320701294
150-159	27.649569857345096	21.60775345747577	27.48273984536644	23.2599368398127
160-169	24.22694108679081	54.923227703364915	1.9997822062506807	18.850049003593597
170-179	20.573886529456605	29.886529456604592	12.126973755853207	37.41261025808559
180-189	21.495371882826962	17.723619732113686	19.715125775890233	41.065882609169115
190-199	48.76794076010019	10.791680278776	1.2749646085157358	39.16541435260808
200-209	21.994337362517697	39.55003811390613	27.739083088315365	10.716541435260808
210-219	49.82315147555265	20.311445061526733	10.12261788086682	19.742785582053795
220-229	22.8848960034847	22.267450724164217	36.80300555374061	18.044647718610477
230-239	39.732113688337144	10.659043885440488	37.26146139605793	12.347381030164433
240-249	28.155940324512684	45.398235870630515	12.378525536317108	14.067298268539693
250-259	48.46673200479146	11.260154633562017	13.21224000871175	27.060873352934774
260-269	14.045736687357072	23.964499618860938	48.11325274964609	13.876510944135903
270-279	37.24730480235217	39.74365675705107	22.019165849940105	0.9898725906566482
280-289	21.95709463138408	1.6920396384623761	32.39464227376674	43.9562234563868
290-299	32.578024610693674	33.5384950451922	11.93226614396167	21.951214200152457
300-309	36.92039638462376	24.396602417510618	14.763802678863117	23.919198519002506
310-319	33.61907873244038	26.542088642055973	15.076336709136449	24.7624959163672
320-329	0.10432320592398997	23.877164325383863	45.96123271262115	30.057279756070997
330-339	19.940760100185127	7.6171185886965045	47.56898617009691	24.87313514102145
340-349	10.633562016770119	10.109332462158337	31.646520744854623	47.610584776216925
350-359	24.144832843297397	25.775019056953063	10.3166721115104	39.763475988239136
360-369	21.955787868888162	24.237612980507457	33.41391702058151	20.39268213002287
370-379	42.70674071654143	33.226396602417516	3.1403680714363493	20.926494609604703
380-389	19.992159425024504	0.13285418708483065	26.30295110530328	53.57203528258739
390-399	27.66133071980834	12.812806272459982	38.028748774910156	21.497114232821517
400-409	2.3382336926930196	27.340302733311557	36.5109441359033	33.81051943809213
410-419	10.36001306762496	20.01023630621801	39.77589023195034	29.853860394206688
420-429	9.678536426004573	20.620929979309594	18.182946749428293	51.51758684525755
430-439	27.199390177501904	30.049656974844822	29.670042469781116	13.080910377872154
440-449	0.32255254274202333	10.383316998802135	27.638897963628445	61.655232494827395
450-459	10.391593161276273	15.56484808885985	35.43003375803114	38.61352499183273
460-469	12.274637917891756	23.170641402591745	20.104540999673308	44.45017967984319
470-479	14.517913535881522	28.44190351736905	40.85658281607318	16.183600130676247
480-489	12.55450288576718	19.860829794184905	22.503321354677123	45.081345965370794
490-499	40.175323968202115	17.441141239246434	20.337580311445063	22.045954481106392
500-509	23.573342045083308	27.24469127736034	30.518131329630837	18.663835347925513
510-519	9.972775781335077	28.104323205923993	30.9511053032778	30.97179570946314
520-529	20.257650005444845	42.06032886856147	4.060328868561473	33.621692257432215
530-539	23.836436894261134	2.189916149406512	26.741152128933898	47.232494827398455
540-549	10.102580855929434	20.59588369813786	29.24708700860285	40.054448437329846
550-559	20.05531961232713	36.53468365457911	22.667755635413265	20.7422410976805
560-569	20.289665686594795	26.960688228247847	29.947947293912662	22.80169879124469
570-579	10.088424262223675	18.379614505063703	20.04682565610367	51.485135576608954
580-589	28.6984645540673	18.994446259392355	20.74333006642709	31.563759120113254
590-599	30.199934661875204	9.673309376020908	20.217575955570073	39.90918000653381
600-602	30.343025155178044	23.486877926603505	20.58586518566917	25.584231732549277
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.5714285714285714
38	0.14285714285714285
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.14285714285714285
46	0.7142857142857142
47	17.571428571428548
48	17.999999999999975
49	87.42857142857136
50	172.9999999999975
51	1566.857142856942
52	4069.4285714310186
53	284.14285714285705
54	149.4285714285696
55	425.71428571430977
56	706.1428571428518
57	339.00000000000927
58	9.28571428571429
59	0.9999999999999998
60	0.2857142857142857
61	0.0
62	0.14285714285714285
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	0.0
350-359	0.0
360-369	0.0
370-379	0.0
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-479	0.0
480-489	0.0
490-499	0.0
500-509	0.0
510-519	0.0
520-529	0.0
530-539	0.0
540-549	0.0
550-559	0.0
560-569	0.0
570-579	0.0
580-589	0.0
590-599	0.0
600-602	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
602	45915.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.0519438092126756
#Duplication Level	Percentage of total
1	0.559729935750844
2	0.29619949907437654
3	0.33975824893825546
4	0.2526407492104977
5	0.1633453119895459
6	0.15681149950996406
7	0.06098224980943047
8	0.12196449961886094
9	0.09800718719372753
>10	0.8929543722095176
>50	0.1437438745508004
>100	1.4483284329739736
>500	1.8817379941195687
>1k	2.831318741152129
>5k	33.02842208428618
>10k+	57.72405531961232
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACTCACACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGA	26504	57.72405531961232	No Hit
ACTCACACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC	8541	18.601764129369485	No Hit
ACTCACACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGC	6624	14.426657954916694	No Hit
ACTCACACTCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGA	1300	2.831318741152129	No Hit
ACTCACACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGC	864	1.8817379941195687	No Hit
ACTCACACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGA	413	0.8994881846890994	No Hit
ACTCACACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGC	252	0.5488402482848742	No Hit
ACTCACACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGG	66	0.1437438745508004	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0	0.0
260-269	0.0	0.0	0.0	0.0	0.0	0.0
270-279	0.0	0.0	0.0	0.0	0.0	0.0
280-289	0.0	0.0	0.0	0.0	0.0	0.0
290-299	0.0	0.0	0.0	0.0	0.0	0.0
300-309	0.0	0.0	0.0	0.0	0.0	0.0
310-319	0.0	0.0	0.0	0.0	0.0	0.0
320-329	0.0	0.0	0.0	0.0	0.0	0.0
330-339	0.0	0.0	0.0	0.0	0.0	0.0
340-349	0.0	0.0	0.0	0.0	0.0	0.0
350-359	0.0	0.0	0.0	0.0	0.0	0.0
360-369	0.0	0.0	0.0	0.0	0.0	0.0
370-379	0.0	0.0	0.0	0.0	0.0	0.0
380-389	0.0	0.0	0.0	0.0	0.0	0.0
390-399	0.0	0.0	0.0	0.0	0.0	0.0
400-409	0.0	0.0	0.0	0.0	0.0	0.0
410-419	0.0	0.0	0.0	0.0	0.0	0.0
420-429	0.0	0.0	0.0	0.0	0.0	0.0
430-439	0.0	0.0	0.0	0.0	0.0	0.0
440-449	0.0	0.0	0.0	0.0	0.0	0.0
450-459	0.0	0.0	0.0	0.0	0.0	0.0
460-469	0.0	0.0	0.0	0.0	0.0	0.0
470-479	0.0	0.0	0.0	0.0	0.0	0.0
480-489	0.0	0.0	0.0	0.0	0.0	0.0
490-499	0.0	0.0	0.0	0.0	0.0	0.0
500-509	0.0	0.0	0.0	0.0	0.0	0.0
510-519	0.0	0.0	0.0	0.0	0.0	0.0
520-529	0.0	0.0	0.0	0.0	0.0	0.0
530-539	0.0	0.0	0.0	0.0	0.0	0.0
540-549	0.0	0.0	0.0	0.0	0.0	0.0
550-559	0.0	0.0	0.0	0.0	0.0	0.0
560-569	0.0	0.0	0.0	0.0	0.0	0.0
570-579	0.0	0.0	0.0	0.0	0.0	0.0
580-589	0.0	0.0	0.0	0.0	0.0	0.0
590-591	0.0	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
