FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR20982524

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20982524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37391
Total Bases22.5 Mbp
Sequences flagged as poor quality0
Sequence length602
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGC799821.390174106068304No Hit
TCCACAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGC655617.5336310876949No Hit
TCCACAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGA559414.960819448530396No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGC24766.621914364419245No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGA21285.691209114492792No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGG12813.4259581182637535No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGC11513.0782808697280095No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCTT11313.0247920622609716No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGACGA9952.661068171485117No Hit
TCCACAACTCCTACGGGAGGCAGCAGTAGGGAATCTTTCACAATGGGCGA6571.7571073252921825No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGGGGAATTTTGCGCAATGGGGGA5871.5698964991575513No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGT4701.2569869754753817No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGA4471.1954748468882888No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC4221.1286138375544918No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGG3730.9975662592602498No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGAGA3560.9521007729132679No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGC3420.9146586076863417No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGG3260.8718675617127115No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGGGGGATATTGGACAATGGGGGA2600.6953544970714878No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGA2470.6605867722179134No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGA2130.5696557995239496No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGA1800.48139926720333776No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGG1750.46802706533657834No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGG1730.4626781845898746No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGA1630.43593378085635576No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGC1580.4225615789895964No Hit
TCCACAACTCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGA1450.3877938541360221No Hit
TCCACAACTCCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGG1400.37442165226926266No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGGGGAATTTTGCGCAATGCTCGA1340.3583750100291514No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGC1090.2915140006953545No Hit
TCCACAACTCCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGA950.2540718354684282No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGC880.2353507528549651No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGAGG750.20058302800139072No Hit
TCCACAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGCCGC740.19790858762803884No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGAGGGATATTGGTCAATGGGGGA600.16046642240111256No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGA590.1577919820277607No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGA520.13907089941429757No Hit
TCCACAACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGAGGA430.11500093605413067No Hit
TCCACAACTCCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGA380.1016287341873713No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)