##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR20982526
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40178
Total Bases	24.1 Mbp
Sequences flagged as poor quality	0
Sequence length	602
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.82286325849967	34.0	34.0	34.0	34.0	34.0
2	33.84740405196874	34.0	34.0	34.0	34.0	34.0
3	33.84364577629548	34.0	34.0	34.0	34.0	34.0
4	33.79794912638758	34.0	34.0	34.0	34.0	34.0
5	37.53312758225895	38.0	38.0	38.0	37.0	38.0
6	37.398327442879186	38.0	38.0	38.0	36.0	38.0
7	37.527129274727464	38.0	38.0	38.0	37.0	38.0
8	37.62063816018717	38.0	38.0	38.0	37.0	38.0
9	37.38984021106078	38.0	38.0	38.0	37.0	38.0
10-19	37.46044352630794	38.0	38.0	38.0	36.6	38.0
20-29	37.531883120115495	38.0	38.0	38.0	37.0	38.0
30-39	37.42043157947135	38.0	38.0	38.0	36.8	38.0
40-49	37.361190203594006	38.0	38.0	38.0	36.9	38.0
50-59	37.30605804171437	38.0	38.0	38.0	36.5	38.0
60-69	37.375250136890834	38.0	38.0	38.0	36.9	38.0
70-79	37.234446712131025	38.0	38.0	38.0	36.2	38.0
80-89	37.33993976803226	38.0	37.9	38.0	36.8	38.0
90-99	37.18319727213899	38.0	38.0	38.0	36.0	38.0
100-109	37.26264373537757	38.0	38.0	38.0	36.6	38.0
110-119	37.188289611230026	38.0	38.0	38.0	36.5	38.0
120-129	36.99183383941461	38.0	37.7	38.0	35.7	38.0
130-139	36.96976454776246	38.0	37.8	38.0	35.6	38.0
140-149	37.03260241923441	38.0	38.0	38.0	35.7	38.0
150-159	36.88533525810145	38.0	38.0	38.0	35.2	38.0
160-169	37.01330330031361	38.0	38.0	38.0	36.1	38.0
170-179	36.8312509333466	38.0	37.9	38.0	35.1	38.0
180-189	36.7280924884265	38.0	37.9	38.0	34.4	38.0
190-199	36.705460699885506	38.0	38.0	38.0	34.4	38.0
200-209	36.24165961471452	38.0	36.9	38.0	33.4	38.0
210-219	36.31193688088008	38.0	37.0	38.0	33.6	38.0
220-229	35.67227338344368	38.0	36.1	38.0	30.7	38.0
230-239	35.55417890387774	38.0	36.4	38.0	29.3	38.0
240-249	35.51473692070287	38.0	36.2	38.0	28.5	38.0
250-259	35.004736422918015	38.0	35.4	38.0	26.6	38.0
260-269	35.12058838170144	38.0	35.6	38.0	26.9	38.0
270-279	34.67933943949426	37.5	34.9	38.0	25.1	38.0
280-289	34.2157225347205	37.1	34.1	38.0	23.0	38.0
290-299	32.80794713524815	36.4	30.8	37.6	21.2	38.0
300-309	30.784377022250982	31.5	30.9	32.3	28.5	32.7
310-319	36.98200258848125	38.0	38.0	38.0	35.5	38.0
320-329	36.84077355766838	38.0	37.7	38.0	35.3	38.0
330-339	37.02720394245607	38.0	37.8	38.0	35.7	38.0
340-349	36.82453581562048	38.0	37.8	38.0	35.1	38.0
350-359	36.6146348748071	38.0	37.5	38.0	34.3	38.0
360-369	36.72452585992334	38.0	37.4	38.0	34.4	38.0
370-379	36.576335307879944	38.0	37.3	38.0	34.5	38.0
380-389	36.56760913932998	38.0	37.6	38.0	34.1	38.0
390-399	36.48221165812136	38.0	37.1	38.0	34.1	38.0
400-409	36.67542436159092	38.0	37.3	38.0	34.7	38.0
410-419	36.39344666235253	38.0	37.0	38.0	34.0	38.0
420-429	36.49875802678082	38.0	37.3	38.0	33.9	38.0
430-439	36.23218676887849	38.0	37.2	38.0	33.0	38.0
440-449	36.0529891980686	38.0	36.9	38.0	31.7	38.0
450-459	36.201575489073626	38.0	37.0	38.0	32.8	38.0
460-469	35.6213176365175	38.0	36.2	38.0	29.9	38.0
470-479	35.79525611030912	38.0	36.7	38.0	31.0	38.0
480-489	35.53172382896112	38.0	36.6	38.0	29.6	38.0
490-499	35.41984419333964	37.9	35.8	38.0	29.2	38.0
500-509	34.95086863457614	38.0	35.3	38.0	26.1	38.0
510-519	34.240086614565186	37.3	34.0	38.0	24.4	38.0
520-529	33.64922345562248	36.9	33.2	38.0	22.8	38.0
530-539	31.294183383941466	34.8	28.4	37.1	19.1	37.1
540-549	32.252710438548455	36.2	30.1	37.4	20.3	37.6
550-559	29.842391358454876	34.0	24.6	36.8	16.6	37.3
560-569	28.090064214246603	30.9	22.2	36.8	14.3	37.0
570-579	27.7375902235054	30.0	21.8	36.2	14.0	37.0
580-589	25.156152620837275	26.9	18.5	32.6	10.4	36.6
590-599	23.537532978246805	25.1	16.7	30.7	8.0	36.2
600-602	13.011805797534306	13.333333333333334	9.666666666666666	17.0	5.333333333333333	20.666666666666668
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	4.0
22	17.0
23	42.0
24	95.0
25	154.0
26	270.0
27	431.0
28	657.0
29	840.0
30	1059.0
31	1403.0
32	1836.0
33	2347.0
34	3380.0
35	6102.0
36	13311.0
37	8230.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	0.01742247000846234	99.96017721140923	0.01742247000846234	0.004977848573846384
8	0.004977848573846384	0.012444621434615959	0.01493354572153915	99.96764398427
9	0.004977848573846384	0.009955697147692767	99.94275474140076	0.04231171287769426
10-19	39.976355219274225	20.01518243815023	10.019164717009309	29.98929762556623
20-29	40.728508138782416	29.243615909204042	10.023893673154463	20.003982278859077
30-39	23.535516949574394	22.275623475533873	30.067947633033004	24.12091194185873
40-49	44.68589775499029	27.14221713375479	11.598636069490766	16.57324904176415
50-59	16.72457563840908	40.00846234257554	17.149435014186867	26.11752700482851
60-69	36.39429538553437	29.859375777788838	0.262830404699089	33.4834984319777
70-79	57.67260689929813	3.879984070884564	18.43546219324008	20.01194683657723
80-89	30.176464731942854	38.31524715018169	24.67370202598437	6.834586091891085
90-99	30.219274229677932	19.635621484394445	32.27537458310518	17.86972970282244
100-109	9.087560356413958	27.55313853352581	35.479117925232714	27.880183184827516
110-119	40.990840758624124	39.44347652944398	18.837921250435564	0.7277614614963412
120-129	36.02319677435412	34.04923092239534	20.06670317088954	9.860869132360994
130-139	27.790830802926976	39.75608541988153	20.787246751953806	11.665837025237693
140-149	13.957638508636567	19.219224451192193	39.766041117029225	27.057095923142015
150-159	27.85056498581313	20.681218577330878	27.724127632037433	23.74408880481856
160-169	21.62924983821992	51.28702274876799	3.5795709094529347	23.504156503559162
170-179	25.766837572801037	28.25377072029469	12.276867937677336	33.702523769226936
180-189	27.90731245955498	18.331425158046695	18.41704415351685	35.34421822888148
190-199	42.89984568669421	14.95694160983623	6.42416247697745	35.71905022649211
200-209	20.235452237542933	38.28886455274031	27.72810991089651	13.747573298820251
210-219	48.34685648862562	20.708845636915722	10.671013987754492	20.273283886704167
220-229	28.848125840011946	19.061177758972573	32.786599631639206	19.304096769376276
230-239	38.21992134999253	14.303598984518892	35.144108716212855	12.332370949275722
240-249	30.68445417890388	44.46089899945244	14.903180845238687	9.951465976404998
250-259	40.97615610533127	17.02822440141371	16.698939718253772	25.296679775001245
260-269	12.573796605107274	22.475981880631192	48.174871820399225	16.775349693862314
270-279	33.913584548758024	40.82408283140027	21.312409776494597	3.9499228433471054
280-289	22.89237891383344	9.238638060630196	36.28055154562198	31.58843147991438
290-299	23.945691672059336	36.56055552790084	20.201353974812086	19.292398825227735
300-309	40.389018866046094	13.505152073273932	23.231868186569766	22.87396087411021
310-319	34.03180845238688	25.89178157200458	15.399721240479863	24.67668873512868
320-329	0.22325650853701032	23.80556523470556	46.064264025088356	29.906914231669074
330-339	19.869331474936534	6.619543033500921	48.697048135795704	24.814077355766837
340-349	10.750410672507343	10.492060331524716	30.06894320274777	48.68858579322017
350-359	23.182089700831302	26.16879884513913	12.168101946338792	38.48100950769078
360-369	21.59141818905869	28.28413559659515	31.68052167853054	18.44392453581562
370-379	38.20971676041614	34.45691672059336	6.128727164119668	21.204639354870825
380-389	20.009706804719	0.2536213848374732	29.73343620887053	50.003235601573
390-399	28.03648763004629	13.588779929314551	39.94599034297377	18.428742097665392
400-409	1.8719199561949327	26.129473841405744	38.86281049330479	33.13579570909453
410-419	11.722086714122156	20.005226741002538	39.73144506944099	28.541241475434315
420-429	8.421026432375928	20.828811787545423	20.8295584648315	49.920603315247156
430-439	24.99825775299915	33.876748469311565	28.291104584598536	12.833889193090748
440-449	0.5358653989745632	12.123052416745482	26.928169645079397	60.41291253920056
450-459	8.667181044352631	12.296281547115337	36.207874956443824	42.82866245208821
460-469	10.582408283140026	26.303698541490366	20.93807556374135	42.175817611628254
470-479	11.201901538155209	30.700881079197572	40.73473044949973	17.362486933147494
480-489	13.21096122255961	22.51331574493504	23.201005525411915	41.07471750709343
490-499	35.41764149534571	22.10662551645179	20.96819154761312	21.507541440589378
500-509	18.085519438498682	27.46901289262781	34.75508985016676	19.690377818706757
510-519	9.52337099905421	29.92284334710538	32.35029120414157	28.20349444969884
520-529	19.639852655682212	39.655035093832446	5.547065558265717	35.15804669221963
530-539	20.769824281945343	3.2632286325849966	28.117128776942607	47.849818308527055
540-549	10.192393847379162	21.811190203594006	28.070088107919755	39.926327841107074
550-559	21.241226541888597	34.70381800985614	23.07705709592314	20.977898352332122
560-569	22.789835233212205	26.21583951416198	26.88884464134601	24.105480611279805
570-579	11.200657076011748	19.857633530787993	20.19737169595301	48.744337697247246
580-589	28.902135497038177	17.885658818258747	20.742197222360495	32.47000846234258
590-599	31.601622778635075	10.006720095574693	20.378565384041018	38.013091741749214
600-602	45.73730233792955	13.203743342127533	31.055967610798614	10.002986709144308
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.14285714285714285
29	0.14285714285714285
30	0.0
31	0.0
32	0.2857142857142857
33	0.5714285714285714
34	0.0
35	0.0
36	0.14285714285714285
37	0.42857142857142855
38	0.0
39	0.0
40	0.0
41	0.14285714285714285
42	0.42857142857142855
43	0.0
44	0.14285714285714285
45	0.0
46	2.999999999999999
47	182.71428571428294
48	203.5714285714252
49	497.71428571432205
50	725.857142857133
51	924.2857142856593
52	3870.5714285736044
53	464.8571428571736
54	58.57142857142906
55	37.428571428571466
56	11.14285714285714
57	1.4285714285714282
58	1.4285714285714282
59	1.2857142857142854
60	1.2857142857142854
61	0.2857142857142857
62	0.14285714285714285
63	0.14285714285714285
64	0.0
65	0.0
66	0.0
67	0.42857142857142855
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	0.0
350-359	0.0
360-369	0.0
370-379	0.0
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-479	0.0
480-489	0.0
490-499	0.0
500-509	0.0
510-519	0.0
520-529	0.0
530-539	0.0
540-549	0.0
550-559	0.0
560-569	0.0
570-579	0.0
580-589	0.0
590-599	0.0
600-602	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
602	40178.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.1299716262631292
#Duplication Level	Percentage of total
1	0.6197421474438748
2	0.3882721887600179
3	0.2762705958484743
4	0.18915824580616256
5	0.21155856438847132
6	0.22400318582308726
7	0.1568022300761611
8	0.019911394295385534
9	0.13440191149385236
>10	0.5052516302454079
>50	0.6023196774354124
>100	1.8418039723231618
>500	1.3564637363731396
>1k	16.723082283836927
>5k	15.304395440290705
>10k+	61.44656279555976
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACGAAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGA	24688	61.44656279555976	No Hit
ACGAAGACTCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGA	6149	15.304395440290705	No Hit
ACGAAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGC	4689	11.670565981382847	No Hit
ACGAAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGA	2030	5.052516302454079	No Hit
ACGAAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGG	545	1.3564637363731396	No Hit
ACGAAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGC	449	1.117527004828513	No Hit
ACGAAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGC	182	0.4529842202200209	No Hit
ACGAAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGA	109	0.27129274727462793	No Hit
ACGAAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGA	99	0.246403504405396	No Hit
ACGAAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGA	87	0.21653641296231768	No Hit
ACGAAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC	56	0.13937976006769873	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0	0.0
260-269	0.002737816715615511	0.0	0.0	0.0	0.0	0.0
270-279	0.006720095574692618	0.0	0.0	0.0	0.0	0.0
280-289	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
290-299	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
300-309	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
310-319	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
320-329	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
330-339	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
340-349	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
350-359	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
360-369	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
370-379	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
380-389	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
390-399	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
400-409	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
410-419	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
420-429	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
430-439	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
440-449	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
450-459	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
460-469	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
470-479	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
480-489	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
490-499	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
500-509	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
510-519	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
520-529	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
530-539	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
540-549	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
550-559	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
560-569	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
570-579	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
580-589	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
590-591	0.007466772860769575	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
