##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR20982527
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	44903
Total Bases	27 Mbp
Sequences flagged as poor quality	0
Sequence length	602
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.856245685143534	34.0	34.0	34.0	34.0	34.0
2	33.845711867803935	34.0	34.0	34.0	34.0	34.0
3	33.84433111373405	34.0	34.0	34.0	34.0	34.0
4	37.34084582321894	38.0	38.0	38.0	37.0	38.0
5	37.54811482529007	38.0	38.0	38.0	37.0	38.0
6	37.37496381088123	38.0	38.0	38.0	37.0	38.0
7	37.57530677237601	38.0	38.0	38.0	37.0	38.0
8	37.701178095004785	38.0	38.0	38.0	37.0	38.0
9	37.23503997505735	38.0	38.0	38.0	37.0	38.0
10-19	37.468322829209626	38.0	38.0	38.0	36.9	38.0
20-29	37.42947687236933	38.0	38.0	38.0	36.7	38.0
30-39	37.37333808431508	38.0	38.0	38.0	36.8	38.0
40-49	37.25697169454156	38.0	38.0	38.0	36.4	38.0
50-59	37.297505734583424	38.0	38.0	38.0	36.6	38.0
60-69	37.27856936062179	38.0	38.0	38.0	36.4	38.0
70-79	37.08702091174308	38.0	37.9	38.0	35.7	38.0
80-89	37.205725675344624	38.0	37.8	38.0	36.6	38.0
90-99	37.09791327973632	38.0	38.0	38.0	35.9	38.0
100-109	37.252056655457324	38.0	38.0	38.0	36.7	38.0
110-119	37.16972362648375	38.0	38.0	38.0	36.5	38.0
120-129	36.93012939001848	38.0	37.4	38.0	35.4	38.0
130-139	36.75542168674698	38.0	37.5	38.0	34.7	38.0
140-149	36.89817606841414	38.0	37.7	38.0	35.1	38.0
150-159	36.79390909293366	38.0	37.8	38.0	34.5	38.0
160-169	36.92572211210832	38.0	38.0	38.0	35.2	38.0
170-179	36.647099302941896	38.0	37.6	38.0	34.2	38.0
180-189	36.74561387880543	38.0	37.8	38.0	34.3	38.0
190-199	36.59543237645592	38.0	37.5	38.0	34.1	38.0
200-209	36.19206734516625	38.0	37.0	38.0	33.7	38.0
210-219	36.00555196757455	38.0	36.7	38.0	32.2	38.0
220-229	35.293552769302714	37.8	35.9	38.0	28.1	38.0
230-239	35.22408302340602	38.0	36.1	38.0	27.5	38.0
240-249	35.26208493864552	38.0	35.9	38.0	27.2	38.0
250-259	34.98277397946684	38.0	35.2	38.0	25.6	38.0
260-269	34.847823085317245	37.9	35.2	38.0	26.0	38.0
270-279	34.15843262142842	37.2	34.0	38.0	24.0	38.0
280-289	33.71691646437878	37.0	33.6	38.0	21.6	38.0
290-299	31.534229338796962	34.9	29.4	36.7	19.3	37.6
300-309	28.984328441306815	29.6	29.6	29.6	28.5	29.6
310-319	36.94179008084093	38.0	38.0	38.0	35.4	38.0
320-329	36.776700888582056	38.0	37.5	38.0	34.9	38.0
330-339	37.06715586931831	38.0	38.0	38.0	36.1	38.0
340-349	36.94395029285348	38.0	37.9	38.0	35.4	38.0
350-359	36.588283633610224	38.0	37.3	38.0	34.0	38.0
360-369	36.75715208337973	38.0	37.5	38.0	34.7	38.0
370-379	36.57784780526914	38.0	37.1	38.0	34.1	38.0
380-389	36.55523907088614	38.0	37.4	38.0	34.3	38.0
390-399	36.463242990446076	38.0	37.0	38.0	34.0	38.0
400-409	36.54160078391199	38.0	37.0	38.0	34.2	38.0
410-419	36.327664075896934	38.0	36.9	38.0	33.8	38.0
420-429	36.518386299356386	38.0	37.1	38.0	34.1	38.0
430-439	36.23675032848585	38.0	37.2	38.0	32.8	38.0
440-449	35.95394071665591	38.0	36.7	38.0	31.7	38.0
450-459	36.05759526089571	38.0	37.0	38.0	32.2	38.0
460-469	35.67459189809144	38.0	36.4	38.0	30.2	38.0
470-479	35.71208827917957	38.0	36.6	38.0	30.1	38.0
480-489	35.05089414961138	37.9	35.5	38.0	26.7	38.0
490-499	35.178629490234506	38.0	35.5	38.0	27.9	38.0
500-509	34.93877469211411	38.0	35.2	38.0	26.2	38.0
510-519	33.66702002093401	37.1	32.9	37.9	22.7	38.0
520-529	33.37754938422823	36.9	32.7	38.0	22.0	38.0
530-539	30.52698037993007	33.7	26.8	37.0	17.7	37.0
540-549	31.950056789078683	36.1	29.2	37.5	19.7	37.7
550-559	29.249660379039263	32.7	23.6	36.7	15.3	37.2
560-569	27.21934169209184	29.3	20.6	35.3	13.0	37.1
570-579	26.613990156559698	28.9	19.7	35.8	12.0	37.0
580-589	24.37587243614012	26.1	18.0	31.3	9.4	36.2
590-599	23.096657238937265	24.6	16.0	30.4	7.7	36.6
600-602	5.32700116547521	5.333333333333333	3.6666666666666665	6.333333333333333	3.6666666666666665	7.666666666666667
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	22.0
23	57.0
24	108.0
25	229.0
26	368.0
27	556.0
28	757.0
29	995.0
30	1305.0
31	1770.0
32	2315.0
33	3086.0
34	4370.0
35	7595.0
36	16133.0
37	5237.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	0.008908090773444982	99.96436763690622	0.013362136160167471	0.013362136160167471
8	0.0	0.013362136160167471	0.006681068080083736	99.97995679575975
9	0.006681068080083736	0.011135113466806226	99.91537313765228	0.06681068080083737
10-19	39.96904438456228	20.02026590650959	10.011135113466805	29.999554595461326
20-29	40.4894995880008	29.245039306950538	10.013362136160167	20.252098968888493
30-39	26.84475424804579	22.16355254660045	30.013362136160165	20.978331069193594
40-49	51.924593011602795	23.554328218604546	11.190343629601585	13.330735140191077
50-59	13.428946840968308	35.301427521546444	13.567913057034051	37.70171258045119
60-69	33.16549005634367	29.009643008262252	0.8139767944235352	37.01089014097054
70-79	53.91666481081442	7.154533104692337	18.96510255439503	19.96369953009821
80-89	29.964590339175558	38.752421887179025	27.062557067456517	4.220430706188896
90-99	30.491949312963502	19.712491370287065	32.3007371445115	17.494822172237935
100-109	8.870008685388504	28.812774202169123	37.88187871634412	24.435338396098256
110-119	42.05487383916442	38.478498095895596	18.6461929047057	0.8204351602342828
120-129	27.16099147050308	38.452441930383266	24.810591719929626	9.575974879184018
130-139	31.899427655167806	44.39480658307908	16.361044919047725	7.344720842705388
140-149	17.152306081998976	25.3270382825201	39.24815713872125	18.27249849675968
150-159	28.495868872903813	20.525131951094583	27.864062534797227	23.114936641204373
160-169	16.83183751642429	53.423601986504245	7.434247154978508	22.310313342092954
170-179	37.45339955014141	24.36830501302808	11.024430438946172	27.15386499788433
180-189	24.575195421241343	19.182014564728416	18.843729817606842	37.3990601964234
190-199	40.631583635837245	17.933100238291427	4.202837226911342	37.23247889895998
200-209	21.292118566688195	37.80660534930851	28.54152283811772	12.359753245885576
210-219	43.9522971739082	15.880898826359042	10.603077745362226	29.563726254370533
220-229	34.515733915328596	15.870654521969579	30.41934837315992	19.194263189541903
230-239	34.609936975257774	11.466049039039708	42.01322851479857	11.910785470903948
240-249	34.84756029663942	40.638710108456	13.274614168318374	11.239115426586196
250-259	45.5501859563949	13.648085873995056	18.93169721399461	21.870030955615437
260-269	12.89735652406298	21.516157049640334	42.374897000200434	23.211589426096253
270-279	26.613366590205555	44.25739928289869	24.84421976259938	4.285014364296372
280-289	36.033449880854285	10.346970135625682	26.72560853395096	26.893971449569072
290-299	18.649310736476405	32.89579760817763	24.480546956773487	23.974344698572477
300-309	34.8382068013273	15.63793065051333	25.595839921608803	23.928022626550565
310-319	33.40534040041868	25.97755161125092	14.426875709863484	26.19023227846692
320-329	0.1006614257399283	24.118210364563613	45.718548872013	30.06257933768345
330-339	19.663051466494444	2.817851813909984	49.06264614836425	28.456450571231322
340-349	10.822439480658307	9.835868427499276	29.978397879874397	49.36329421196802
350-359	27.85537714629312	22.21922811393448	16.41137563191769	33.51401910785471
360-369	21.152929648353116	28.43930249649244	31.410373471705675	18.997394383448768
370-379	44.09705364897668	30.517782776206488	4.504153397323119	20.881010177493707
380-389	20.00734917488809	0.12961272075362448	29.144155178941272	50.718882925417006
390-399	28.39721176758791	8.93169721399461	39.110749838540855	23.560341179876623
400-409	2.127474778967998	27.359641894750908	33.952965280716214	36.559918045564885
410-419	11.158274502817184	20.047435583368596	39.98218381845311	28.812106095361113
420-429	8.862659510500412	20.569895107231144	20.86074427098412	49.706701111284325
430-439	21.408814555820324	37.313987929537	28.472262432354185	12.804935082288488
440-449	0.7872525221032002	10.91597443377948	22.77620648954413	65.5205665545732
450-459	8.998730597064784	12.631004609936975	31.949535665768437	46.420729127229805
460-469	10.894372313653875	22.772865955504088	20.74761151816137	45.58515021268067
470-479	11.40502861724161	30.94581653787052	39.44480324254504	18.20435160234283
480-489	18.291873594191923	29.40248981137118	16.972362648375388	35.33327394606151
490-499	32.409193149678195	20.93557223348106	20.991470503084425	25.663764113756322
500-509	15.404093267710397	18.513016947642697	40.82934325100773	25.253546533639177
510-519	10.056343674142038	33.403781484533326	31.34601251586754	25.193862325457093
520-529	19.196712914504598	40.24786762577111	2.0464111529296485	38.50900830679465
530-539	25.73725586263724	2.1397234037814847	27.937331581408813	44.18568915217246
540-549	9.927844464735095	25.52234817272788	24.49836313832038	40.051444224216645
550-559	21.196801995412333	36.12052646816471	21.77271006391555	20.909961472507405
560-569	26.243235418568915	23.073068614569184	19.45059350154778	31.233102465314122
570-579	15.284502149076898	23.409571743536066	20.70396187337149	40.60196423401555
580-589	28.936596663920007	14.459390241186556	20.595505868204796	36.00850722668864
590-599	35.060909070663435	9.827183038995168	20.250540053003142	34.861367837338264
600-602	35.19289728228998	16.719001699960657	27.514865376478188	20.573235641271186
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.2857142857142857
46	0.7142857142857142
47	24.571428571428523
48	24.142857142857096
49	297.4285714285736
50	556.1428571428859
51	730.1428571428463
52	4163.142857145218
53	1293.5714285712897
54	120.57142857142756
55	393.0000000000185
56	85.7142857142857
57	7.000000000000006
58	3.142857142857142
59	0.7142857142857142
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	0.0
350-359	0.0
360-369	0.0
370-379	0.0
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-479	0.0
480-489	0.0
490-499	0.0
500-509	0.0
510-519	0.0
520-529	0.0
530-539	0.0
540-549	0.0
550-559	0.0
560-569	0.0
570-579	0.0
580-589	0.0
590-599	0.0
600-602	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
602	44903.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.080106006280204
#Duplication Level	Percentage of total
1	0.5745718548872013
2	0.26278867781662696
3	0.2204752466427633
4	0.35632363093779923
5	0.11135113466806228
6	0.21379417856267954
7	0.21824822394940205
8	0.17816181546889961
9	0.12025922544150724
>10	1.1914571409482662
>50	0.1759347927755384
>100	1.115738369373984
>500	0.0
>1k	17.012226354586556
>5k	0.0
>10k+	78.24866935394071
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTAGCCACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGC	23146	51.54666726053938	No Hit
TTAGCCACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGA	11990	26.702002093401333	No Hit
TTAGCCACTCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGA	4664	10.386833841836848	No Hit
TTAGCCACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC	1888	4.204618845066031	No Hit
TTAGCCACTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGA	1087	2.4207736676836737	No Hit
TTAGCCACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGA	193	0.42981537981872037	No Hit
TTAGCCACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGA	160	0.35632363093779923	No Hit
TTAGCCACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGA	148	0.3295993586174643	No Hit
TTAGCCACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGA	79	0.1759347927755384	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0	0.0
260-269	0.0	0.0	0.0	0.0	0.0	0.0
270-279	0.0	0.0	0.0	0.0	0.0	0.0
280-289	0.0	0.0	0.0	0.0	0.0	0.0
290-299	0.0	0.0	0.0	0.0	0.0	0.0
300-309	0.0	0.0	0.0	0.0	0.0	0.0
310-319	0.0	0.0	0.0	0.0	0.0	0.0
320-329	0.0	0.0	0.0	0.0	0.0	0.0
330-339	0.0	0.0	0.0	0.0	0.0	0.0
340-349	0.0	0.0	0.0	0.0	0.0	0.0
350-359	0.0	0.0	0.0	0.0	0.0	0.0
360-369	0.0	0.0	0.0	0.0	0.0	0.0
370-379	0.0	0.0	0.0	0.0	0.0	0.0
380-389	0.0	0.0	0.0	0.0	0.0	0.0
390-399	0.0	0.0	0.0	0.0	0.0	0.0
400-409	0.0	0.0	0.0	0.0	0.0	0.0
410-419	0.0	0.0	0.0	0.0	0.0	0.0
420-429	0.0	0.0	0.0	0.0	0.0	0.0
430-439	0.0	0.0	0.0	0.0	0.0	0.0
440-449	0.0	0.0	0.0	0.0	0.0	0.0
450-459	0.0	0.0	0.0	0.0	0.0	0.0
460-469	0.0	0.0	0.0	0.0	0.0	0.0
470-479	0.0	0.0	0.0	0.0	0.0	0.0
480-489	0.0	0.0	0.0	0.0	0.0	0.0
490-499	0.0	0.0	0.0	0.0	0.0	0.0
500-509	0.0	0.0	0.0	0.0	0.0	0.0
510-519	0.0	0.0	0.0	0.0	0.0	0.0
520-529	0.0	0.0	0.0	0.0	0.0	0.0
530-539	0.0	0.0	0.0	0.0	0.0	0.0
540-549	0.0	0.0	0.0	0.0	0.0	0.0
550-559	0.0	0.0	0.0	0.0	0.0	0.0
560-569	0.0	0.0	0.0	0.0	0.0	0.0
570-579	0.0	0.0	0.0	0.0	0.0	0.0
580-589	0.0	0.0	0.0	0.0	0.0	0.0
590-591	0.0	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
