FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR20982530

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20982530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55031
Total Bases33.1 Mbp
Sequences flagged as poor quality0
Sequence length602
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACCAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGA3353360.93474587050935No Hit
TGACCAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGC787614.311933273972851No Hit
TGACCAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGC51269.314749868256074No Hit
TGACCAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGG29795.413312496592829No Hit
TGACCAACTCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGA23064.190365430393778No Hit
TGACCAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGA11012.0006905198887903No Hit
TGACCAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGC2300.41794624847813056No Hit
TGACCAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGA1690.307099634751322No Hit
TGACCAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGG1470.26712216750558776No Hit
TGACCAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGA1300.23623048827024767No Hit
TGACCAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC960.17444712979956753No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)