##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR20982531
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	44716
Total Bases	26.9 Mbp
Sequences flagged as poor quality	0
Sequence length	602
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.659137668843364	38.0	38.0	38.0	37.0	38.0
2	37.59987476518472	38.0	38.0	38.0	37.0	38.0
3	37.441296180338135	38.0	38.0	38.0	37.0	38.0
4	37.57064585383308	38.0	38.0	38.0	37.0	38.0
5	36.95672689864925	38.0	38.0	38.0	35.0	38.0
6	36.77880400751409	38.0	37.0	38.0	35.0	38.0
7	37.25299669022274	38.0	38.0	38.0	36.0	38.0
8	37.6081939350568	38.0	38.0	38.0	37.0	38.0
9	37.57310582341891	38.0	38.0	38.0	37.0	38.0
10-19	37.54529027641112	38.0	38.0	38.0	36.9	38.0
20-29	37.40570712943912	38.0	38.0	38.0	36.6	38.0
30-39	37.30346855711603	38.0	38.0	38.0	36.6	38.0
40-49	37.24504875212452	38.0	38.0	38.0	36.2	38.0
50-59	37.28874228464085	38.0	38.0	38.0	36.6	38.0
60-69	37.27351283656857	38.0	38.0	38.0	36.3	38.0
70-79	37.02893147866535	38.0	37.7	38.0	35.3	38.0
80-89	37.13033589766526	38.0	38.0	38.0	36.2	38.0
90-99	37.10114276768941	38.0	38.0	38.0	36.0	38.0
100-109	37.165857858484664	38.0	37.9	38.0	36.5	38.0
110-119	37.0603475266124	38.0	38.0	38.0	36.0	38.0
120-129	37.147848644780396	38.0	38.0	38.0	36.1	38.0
130-139	37.00534484300921	38.0	37.9	38.0	35.6	38.0
140-149	37.02391314071026	38.0	37.8	38.0	35.8	38.0
150-159	36.84905179354146	38.0	37.7	38.0	35.5	38.0
160-169	37.019228016817245	38.0	38.0	38.0	35.7	38.0
170-179	36.640048304857324	38.0	37.4	38.0	34.3	38.0
180-189	36.86909383665802	38.0	37.9	38.0	35.0	38.0
190-199	36.5573262366938	38.0	37.6	38.0	34.1	38.0
200-209	36.14040611861525	38.0	36.7	38.0	32.3	38.0
210-219	36.24652026120404	38.0	36.9	38.0	33.8	38.0
220-229	35.53852759638608	38.0	35.9	38.0	30.3	38.0
230-239	35.44532158511495	38.0	36.5	38.0	28.0	38.0
240-249	35.59318588424725	38.0	36.2	38.0	29.8	38.0
250-259	34.78702925127472	37.6	35.0	38.0	26.6	38.0
260-269	34.6096520261204	37.7	34.7	38.0	25.3	38.0
270-279	34.171108775382415	37.1	34.1	38.0	24.1	38.0
280-289	33.52892700599338	37.0	33.4	38.0	21.7	38.0
290-299	23.100122998479293	25.8	20.9	26.7	14.5	27.2
300-309	29.913395652562844	30.8	30.6	30.8	28.5	30.8
310-319	36.66442883978889	38.0	37.5	38.0	34.8	38.0
320-329	36.75072010018785	38.0	37.6	38.0	35.0	38.0
330-339	36.933348242239916	38.0	37.5	38.0	35.6	38.0
340-349	36.662002415242874	38.0	37.5	38.0	34.4	38.0
350-359	36.76148134895787	38.0	37.6	38.0	34.7	38.0
360-369	36.78688388943554	38.0	37.5	38.0	34.8	38.0
370-379	36.63780973253422	38.0	37.3	38.0	34.6	38.0
380-389	36.456172287324456	38.0	37.1	38.0	34.1	38.0
390-399	36.367861615529115	38.0	37.1	38.0	33.9	38.0
400-409	36.38244028982915	38.0	37.0	38.0	33.5	38.0
410-419	36.15670900796136	38.0	37.0	38.0	32.8	38.0
420-429	36.40859423919849	38.0	37.0	38.0	33.9	38.0
430-439	36.20852044011092	38.0	37.2	38.0	32.7	38.0
440-449	36.08417345021916	38.0	36.7	38.0	32.1	38.0
450-459	36.07217774398426	38.0	36.9	38.0	32.2	38.0
460-469	35.69697871008141	38.0	36.6	38.0	30.5	38.0
470-479	35.415560425798375	37.9	36.0	38.0	29.1	38.0
480-489	34.91511539493693	37.9	35.3	38.0	26.3	38.0
490-499	34.83644109491009	37.7	35.0	38.0	26.6	38.0
500-509	33.93984927095447	37.1	34.2	38.0	22.7	38.0
510-519	33.34095849360408	37.0	32.2	37.9	22.1	38.0
520-529	32.60131049288845	36.2	31.1	37.7	21.2	37.7
530-539	29.202435369889976	32.3	24.3	36.9	14.9	37.0
540-549	30.919476250111813	34.8	26.7	37.5	17.8	37.8
550-559	28.273747651847213	31.1	22.1	36.8	13.7	37.1
560-569	25.851435727703727	27.6	18.7	34.1	11.2	37.0
570-579	25.915075140889165	27.8	19.1	34.1	11.2	36.6
580-589	23.29847705519277	24.3	17.3	30.7	9.2	35.4
590-599	17.62688523123714	18.2	12.4	22.5	6.2	27.7
600-602	2.0	2.0	2.0	2.0	2.0	2.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	8.0
22	32.0
23	78.0
24	148.0
25	281.0
26	406.0
27	605.0
28	827.0
29	1156.0
30	1471.0
31	2000.0
32	2444.0
33	3344.0
34	5435.0
35	10015.0
36	16052.0
37	414.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.013418015922712228	99.94856427229627	0.026836031845424456	0.011181679935593523
8	0.0	0.0	0.00894534394847482	99.99105465605153
9	0.0	0.006709007961356114	99.94856427229627	0.04472671974237409
10-19	39.97159853296359	20.02035065748278	10.007603542356204	30.000447267197423
20-29	41.4788889882816	28.509482064585384	10.012299847929153	19.999329099203862
30-39	22.16745683871545	22.04334019143036	30.010063511942036	25.77913945791216
40-49	48.04275874407371	22.039314786653545	10.572278379103677	19.345648090169068
50-59	19.359960640486626	38.90441899991055	12.061454512926021	29.674165846676804
60-69	37.79228911351641	28.528043653278466	1.6137400483048574	32.06592718490026
70-79	49.474013775829675	9.646211646837822	20.870158332587888	20.00961624474461
80-89	32.05362733697111	32.1175865462027	27.000849807675102	8.827936309151086
90-99	31.552688075856516	21.876509526791306	32.05519277216209	14.515609625190088
100-109	9.49548260130602	29.32261382950174	40.15967438948028	21.02222917971196
110-119	49.75847571339118	32.31505501386528	16.39122461758655	1.535244655156991
120-129	19.41810537615171	48.331693353609445	23.58529385454871	8.664907415690132
130-139	18.93952947490831	34.340280883799984	34.17076661597638	12.549423025315324
140-149	10.975937024778602	29.122461758654623	36.437740406118614	23.46386081044816
150-159	27.917747562393775	20.082073530727257	20.78405939708382	31.216119509795153
160-169	23.425172197871007	56.0231237141068	3.2675105107791396	17.284193577243045
170-179	20.817157169693175	29.873647016727794	10.64920833706056	38.659987476518474
180-189	22.30745147150908	17.981259504427946	19.449637713570088	40.261651310492894
190-199	48.08323642544055	11.274711512657662	1.8449771893729312	38.797074872528846
200-209	21.523615708023975	39.361749709276324	27.94033455586367	11.17430002683603
210-219	49.83138026657125	20.501610161910726	10.103542356203596	19.56346721531443
220-229	22.08896144556758	29.81371321227301	29.48608999016012	18.611235351999284
230-239	39.79895339475803	10.12098577690312	37.97745773324984	12.102603095089007
240-249	28.75436085517488	32.57491725556847	11.284327757402272	27.38639413185437
250-259	48.216074783075406	11.338670721889256	27.17483674747294	13.270417747562394
260-269	11.708784327757401	22.808837999821094	47.28799534842115	18.194382324000358
270-279	37.21128902406298	39.832945701762235	21.08685928973969	1.8689059844351013
280-289	21.11458985597996	9.904955720547456	13.436130244207888	55.54432417926469
290-299	29.321942928705607	26.323240003578135	16.934877896055102	27.419939171661152
300-309	46.40330083191699	4.637937203685482	34.239198497182215	14.719563467215313
310-319	33.770238840683426	26.111682619196706	15.069326415600681	25.048752124519186
320-329	0.09057160747830754	23.530279989265587	46.334198049915024	30.044950353341086
330-339	19.933357187583862	7.741300653010108	49.17210841756866	23.153233741837372
340-349	10.697736827981036	10.026836031845425	30.052553895697287	49.22287324447625
350-359	22.87548081223723	27.55501386528312	10.904821540388228	38.664683782091416
360-369	20.552598622417033	27.20077824492352	31.359915913766883	20.886707218892568
370-379	39.21705877090974	36.59092942123625	3.5794793809821988	20.612532426871812
380-389	19.96913856337776	0.18964129170766617	23.380445478128635	56.46077466678594
390-399	27.479202075319797	13.418910457107074	38.55443241792647	20.54745504964666
400-409	2.044011092226496	20.69572412559263	30.362733697110656	46.89753108507022
410-419	17.804365327846856	12.78334376956794	39.95460237946149	29.457688523123714
420-429	9.423248948922087	20.687002415242866	19.789784417210843	50.0999642186242
430-439	19.97987297611593	37.75382413453797	29.41899991054656	12.847302978799535
440-449	0.04964665891403525	10.752527059665445	30.22452813310672	58.9732981483138
450-459	10.821853475266124	13.624206100724573	35.94105018337955	39.61289024062975
460-469	11.524957509616245	21.64415421772967	22.93742731908042	43.893460953573666
470-479	12.14732981483138	31.260622595938813	38.531621790857855	18.06042579837195
480-489	12.688746757312819	19.40916003220324	13.8583504785759	54.04374273190804
490-499	45.23526254584489	12.401153949369354	21.22551212094105	21.138071383844707
500-509	29.99754003041417	13.491815010287144	37.03618391627158	19.474461043027105
510-519	9.988147419268271	28.462966276053315	32.069281688880935	29.479604615797477
520-529	18.280704893103138	47.2871008140263	0.7453707845066643	33.686823508363894
530-539	29.72045800161016	8.074738348689507	14.352133464531711	47.85267018516862
540-549	10.159674389480275	20.457107075767063	29.14527238572323	40.23794614902943
550-559	20.31688880937472	29.406252795419984	29.53126397709992	20.745594418105377
560-569	20.44011092226496	26.891940245102425	30.935906610609177	21.732042222023438
570-579	10.10756776098041	18.851194203417123	20.113829501744345	50.927408533858134
580-589	28.812505590839965	18.75055908399678	20.730163699794257	31.706771625368997
590-599	34.699436443331244	5.104884157795867	24.773235530906163	35.42244386796673
600-602	25.29519635029967	24.589259623699196	22.904553180069772	27.21099084593136
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.5714285714285714
34	0.2857142857142857
35	0.0
36	0.0
37	0.0
38	0.14285714285714285
39	0.0
40	0.0
41	0.0
42	0.0
43	0.5714285714285714
44	2.5714285714285707
45	0.14285714285714285
46	0.2857142857142857
47	4.428571428571428
48	8.71428571428572
49	52.28571428571464
50	330.71428571429357
51	192.5714285714255
52	2286.000000000014
53	3343.5714285728855
54	313.5714285714335
55	752.2857142856984
56	201.42857142856812
57	9.428571428571432
58	1.4285714285714282
59	0.5714285714285714
60	0.0
61	0.0
62	0.14285714285714285
63	0.2857142857142857
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	0.0
350-359	0.0
360-369	0.0
370-379	0.0
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-479	0.0
480-489	0.0
490-499	0.0
500-509	0.0
510-519	0.0
520-529	0.0
530-539	0.0
540-549	0.0
550-559	0.0
560-569	0.0
570-579	0.0
580-589	0.0
590-599	0.0
600-602	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
602	44716.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	0.8676983630020575
#Duplication Level	Percentage of total
1	0.47186689328204673
2	0.24599695858305753
3	0.1811432149566151
4	0.1431255031755971
5	0.15654351909830932
6	0.1341801592271223
7	0.14088916718847838
8	0.05367206369084891
9	0.14088916718847838
>10	1.015296538151892
>50	0.3265050541193309
>100	2.0261204043295464
>500	0.0
>1k	9.18686823508364
>5k	14.133643438590212
>10k+	71.64325968333483
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACCTGAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGA	32036	71.64325968333483	No Hit
ACCTGAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC	6320	14.133643438590212	No Hit
ACCTGAACTCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGA	2374	5.309061633419805	No Hit
ACCTGAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGA	1734	3.8778066016638335	No Hit
ACCTGAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGC	379	0.8475713391179892	No Hit
ACCTGAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGC	294	0.6574827802128992	No Hit
ACCTGAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGA	233	0.5210662849986581	No Hit
ACCTGAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGA	85	0.19008855890508988	No Hit
ACCTGAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGG	61	0.13641649521424098	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0	0.0
260-269	0.0011181679935593524	0.0	0.0	0.0	0.0	0.0
270-279	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
280-289	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
290-299	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
300-309	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
310-319	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
320-329	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
330-339	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
340-349	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
350-359	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
360-369	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
370-379	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
380-389	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
390-399	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
400-409	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
410-419	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
420-429	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
430-439	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
440-449	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
450-459	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
460-469	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
470-479	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
480-489	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
490-499	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
500-509	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
510-519	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
520-529	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
530-539	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
540-549	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
550-559	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
560-569	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
570-579	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
580-589	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
590-591	0.002236335987118705	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
