FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR20982540

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20982540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34759
Total Bases20.9 Mbp
Sequences flagged as poor quality0
Sequence length602
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGA3046887.65499582841854No Hit
TCCCGAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGA10943.147386288443281No Hit
TCCCGAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGC6241.79521850455997No Hit
TCCCGAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGC5131.4758767513449753No Hit
TCCCGAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGC3761.0817342271066486No Hit
TCCCGAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGG3661.0529646997899824No Hit
TCCCGAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGG1160.33372651687332777No Hit
TCCCGAACTCCTACGGGAGGCAGCAGTAGGGAATCTTTCACAATGGGCGA770.22152536033832965No Hit
TCCCGAACTCCTACGGGAGGCATCAGTAGGGAATCTTCCACAATGGGCGA530.1524784947783308No Hit
TCCCGAACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGA490.1409706838516643No Hit
TCCCGAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC390.11220115653499813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)