##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR20982540
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34759
Total Bases	20.9 Mbp
Sequences flagged as poor quality	0
Sequence length	602
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.8408469748842	34.0	34.0	34.0	34.0	34.0
2	33.78155297908455	34.0	34.0	34.0	34.0	34.0
3	33.73586696970569	34.0	34.0	34.0	34.0	34.0
4	37.552835236917055	38.0	38.0	38.0	37.0	38.0
5	37.48421991426681	38.0	38.0	38.0	37.0	38.0
6	37.28588279294571	38.0	38.0	38.0	36.0	38.0
7	37.462585229724674	38.0	38.0	38.0	37.0	38.0
8	37.62588106677407	38.0	38.0	38.0	37.0	38.0
9	37.538018930348976	38.0	38.0	38.0	37.0	38.0
10-19	37.47383699185822	38.0	38.0	38.0	37.0	38.0
20-29	37.4597169078512	38.0	38.0	38.0	36.7	38.0
30-39	37.404942604793	38.0	38.0	38.0	36.7	38.0
40-49	37.39238758307201	38.0	38.0	38.0	37.0	38.0
50-59	37.23466728041658	38.0	37.9	38.0	36.3	38.0
60-69	37.31590667165339	38.0	38.0	38.0	36.7	38.0
70-79	37.230084294715034	38.0	38.0	38.0	36.2	38.0
80-89	37.346278661641584	38.0	38.0	38.0	36.8	38.0
90-99	37.17043067982393	38.0	38.0	38.0	36.0	38.0
100-109	37.23295549354124	38.0	38.0	38.0	36.6	38.0
110-119	37.1993181622026	38.0	38.0	38.0	36.8	38.0
120-129	36.96507954774303	38.0	37.7	38.0	35.6	38.0
130-139	36.920762967864434	38.0	37.8	38.0	35.3	38.0
140-149	37.06144020253747	38.0	37.9	38.0	35.8	38.0
150-159	36.874058517218565	38.0	38.0	38.0	35.2	38.0
160-169	36.99560401622601	38.0	38.0	38.0	35.7	38.0
170-179	36.80722402830922	38.0	37.8	38.0	35.0	38.0
180-189	36.86020886676832	38.0	37.9	38.0	35.1	38.0
190-199	36.61823700336603	38.0	37.7	38.0	34.4	38.0
200-209	36.11384965045024	38.0	36.7	38.0	32.4	38.0
210-219	36.34184527748209	38.0	37.0	38.0	33.7	38.0
220-229	35.62625794758192	37.6	35.9	38.0	30.6	38.0
230-239	35.47746482925287	38.0	36.4	38.0	29.3	38.0
240-249	35.304007595155205	38.0	35.9	38.0	27.9	38.0
250-259	34.591538882016174	37.8	34.8	38.0	24.9	38.0
260-269	34.85153773123508	37.8	35.1	38.0	26.2	38.0
270-279	34.43339854426191	37.5	34.5	38.0	24.5	38.0
280-289	34.107810926666474	37.2	34.3	38.0	22.8	38.0
290-299	30.905828706234352	34.0	28.1	35.9	19.2	36.7
300-309	28.85402341839524	29.6	29.3	29.6	28.1	29.6
310-319	36.8755890560718	38.0	38.0	38.0	35.2	38.0
320-329	36.714456687476634	38.0	37.4	38.0	34.8	38.0
330-339	37.04028021519606	38.0	37.9	38.0	36.0	38.0
340-349	36.836922811358214	38.0	37.7	38.0	35.2	38.0
350-359	36.57184901752065	38.0	37.3	38.0	34.1	38.0
360-369	36.54540982191662	38.0	37.2	38.0	34.1	38.0
370-379	36.4527575591933	38.0	37.0	38.0	34.0	38.0
380-389	36.47186915618976	38.0	37.2	38.0	33.6	38.0
390-399	36.4788946747605	38.0	37.1	38.0	33.9	38.0
400-409	36.66383095025749	38.0	37.1	38.0	34.7	38.0
410-419	36.284153744353986	38.0	36.9	38.0	33.3	38.0
420-429	36.468770678097755	38.0	37.1	38.0	33.8	38.0
430-439	36.12177853217871	38.0	36.9	38.0	32.1	38.0
440-449	35.957662763600794	38.0	36.9	38.0	31.5	38.0
450-459	36.19317586812049	38.0	37.0	38.0	32.8	38.0
460-469	35.57413331798959	38.0	36.3	38.0	29.1	38.0
470-479	35.674751862826895	38.0	36.4	38.0	30.2	38.0
480-489	35.57305158376248	38.0	36.5	38.0	29.5	38.0
490-499	35.32657153542968	37.9	35.6	38.0	28.9	38.0
500-509	34.68873097615006	37.8	35.0	38.0	25.6	38.0
510-519	34.06239822779712	37.2	33.7	37.9	23.9	38.0
520-529	33.45373572312207	36.7	32.5	38.0	22.7	38.0
530-539	31.136741563336113	34.6	28.0	37.0	18.9	37.0
540-549	32.223677320981615	36.0	29.5	37.4	19.9	37.7
550-559	29.36447538766938	32.5	24.1	36.8	15.5	37.2
560-569	27.246937483817135	29.4	20.6	35.2	13.4	37.0
570-579	27.526988693575767	29.4	22.0	35.9	13.6	37.0
580-589	24.594925055381346	26.0	17.8	31.5	9.9	36.2
590-599	23.262657153542968	24.2	16.3	30.9	7.7	36.0
600-602	4.798258484613098	3.6666666666666665	3.6666666666666665	5.666666666666667	3.6666666666666665	7.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	7.0
22	17.0
23	39.0
24	89.0
25	154.0
26	295.0
27	377.0
28	573.0
29	758.0
30	989.0
31	1274.0
32	1648.0
33	2315.0
34	3344.0
35	5945.0
36	12730.0
37	4205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.017261716389999713	99.95396875629334	0.02013866912166633	0.008630858194999856
8	0.0	0.0	0.005753905463333237	99.99424609453666
9	0.014384763658333093	0.0	99.94533789809833	0.04027733824333266
10-19	39.974970511234496	20.010357029834	10.020138669121666	29.994533789809836
20-29	40.46894329526166	29.499122529416844	10.027043355677666	20.004890819643833
30-39	20.73160907966282	20.489945050202827	30.052935930262663	28.725509939871692
40-49	40.75520009206249	29.8489599815875	10.036824994965333	19.359014931384678
50-59	19.51379498834834	39.35268563537501	19.85787853505567	21.275640841220977
60-69	39.16424523145084	29.783940849851838	0.32998647832216116	30.721827440375154
70-79	58.71170056675968	1.289162519059812	19.970942777410166	20.028194136770335
80-89	30.2787767196985	39.898155873299004	20.109036508530163	9.714030898472338
90-99	30.33084956414166	19.385195201242844	30.52245461607066	19.761500618544837
100-109	10.63091573405449	29.03391927270635	31.31764435110331	29.017520642135853
110-119	41.00233033171265	39.27759716907851	19.378290514686842	0.3417819845219943
120-129	39.613625248137176	30.854742656578154	19.774446905837337	9.757185189447338
130-139	29.82536896918784	39.219770419172015	20.401334906067493	10.553525705572657
140-149	10.780804971374321	11.766448977243304	48.1653672430162	29.287378808366178
150-159	29.734169567594005	20.57481515578699	29.432664921315343	20.258350355303662
160-169	20.514111453148825	58.60439022986852	0.8768951926119853	20.004603124370668
170-179	20.811300670329985	29.578814120084008	10.544031761558157	39.06585344802785
180-189	20.670329986478322	19.741074254150003	19.976408987600333	39.61218677177134
190-199	49.229264363186516	10.501740556402659	0.7762018470036537	39.49279323340718
200-209	20.392416352599327	39.820190454270836	29.490203975948674	10.297189217181161
210-219	49.799476394602834	19.746252769067006	10.320204839034494	20.134065997295664
220-229	21.137834805374148	20.291147616444665	39.03449466325269	19.536522914928508
230-239	39.55205845967951	10.681262406858655	39.23185362064501	10.534825512816823
240-249	29.9542564515665	48.815846255646015	10.84467332201732	10.385223970770161
250-259	48.902730228142346	10.967231508386318	11.310739664547311	28.81929859892402
260-269	11.351592393336977	20.904226243562817	57.08708535918755	10.657096003912656
270-279	39.42115711038868	39.761788313818	20.312437066658994	0.5046175091343249
280-289	20.552950315026326	1.418337696711643	38.500244540982195	39.528467447279844
290-299	29.435254178773846	39.42662332057884	11.107914496964815	20.0302080036825
300-309	36.966541039730714	24.995828418539084	23.9937857820996	14.0438447596306
310-319	33.31252337524094	26.526079576512558	14.298455076383096	25.8629419718634
320-329	0.15736931442216404	24.216461923530595	45.59998849218908	30.026180269858166
330-339	19.915992980235334	9.465749877729507	48.765499582841855	21.852757559193304
340-349	10.605598550015822	10.193331223567997	30.273885900054665	48.92718432636152
350-359	20.85071492275382	29.037659311257517	10.48447884001266	39.62714692597601
360-369	20.327109525590494	29.673178169682675	29.932679306079002	20.067032998647832
370-379	38.986161857360685	38.94904916712218	0.8285623867199862	21.236226588797148
380-389	20.018987888029	0.12111971000316464	29.627722316522338	50.232170085445496
390-399	29.622256106332173	10.052072844443167	40.035386518599495	20.29028453062516
400-409	0.1683017348024972	29.44733738024684	39.63203774561984	30.752323139330823
410-419	10.084582410310999	20.027331050950835	39.841192209212004	30.046894329526165
420-429	9.966915043585834	20.588049138352655	19.13116027503668	50.313875543024835
430-439	29.430651054403178	29.71892171811617	29.938145516269167	10.912281711211484
440-449	0.3837854944043269	10.210017549411663	29.362754969935846	60.04344198624817
450-459	10.11709197617883	10.97384849966915	39.241922955205844	39.66713656894617
460-469	10.748870796052822	21.273051583762477	20.669466900658822	47.30861071952588
470-479	11.353606260249144	29.42604793003251	39.91081446531833	19.30953134440001
480-489	10.521591530251158	20.341781984521994	20.96090221237665	48.175724272850196
490-499	38.609281049512354	20.378031588940992	20.409678068989326	20.603009292557324
500-509	20.003164648004834	28.88489312120602	30.415719669725828	20.696222561063323
510-519	9.57334790989384	30.083143933945166	30.41629506027216	29.92721309588883
520-529	19.452228199890676	47.35406657268621	1.6323829799476397	31.561322247475477
530-539	20.29661382663483	0.9862193964153169	29.8265197502805	48.890647026669356
540-549	9.919157628240168	20.823383871802985	29.204810264967346	40.052648234989505
550-559	19.776173077476336	38.265485198078196	21.146465663569145	20.81187606087632
560-569	20.159670876607496	28.689835725999025	29.287666503639343	21.862826893754136
570-579	10.218648407606663	20.32567104922466	20.044305072067665	49.41137547110101
580-589	28.787076728329353	18.909347219425186	20.727005955292157	31.576570096953304
590-599	30.49713743203199	9.822492016456168	20.29459995972266	39.385770591789175
600-602	44.3501443271287	15.675556450607516	15.48375960182974	24.49053962043404
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.5714285714285714
47	29.28571428571422
48	77.85714285714306
49	8.857142857142863
50	22.857142857142815
51	1482.4285714283897
52	3984.0000000023306
53	193.2857142857112
54	150.5714285714267
55	27.857142857142797
56	6.142857142857147
57	3.857142857142856
58	1.9999999999999993
59	0.9999999999999998
60	1.9999999999999993
61	1.7142857142857137
62	0.7142857142857142
63	0.7142857142857142
64	0.5714285714285714
65	0.0
66	0.14285714285714285
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	0.0
350-359	0.0
360-369	0.0
370-379	0.0
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-479	0.0
480-489	0.0
490-499	0.0
500-509	0.0
510-519	0.0
520-529	0.0
530-539	0.0
540-549	0.0
550-559	0.0
560-569	0.0
570-579	0.0
580-589	0.0
590-599	0.0
600-602	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
602	34759.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	0.9378865905233177
#Duplication Level	Percentage of total
1	0.5236053971633247
2	0.16686325843666389
3	0.1985097384849967
4	0.17261716389999712
5	0.18700192755833023
6	0.20714059667999654
7	0.2013866912166633
8	0.16110935297333065
9	0.10357029833999827
>10	1.162288903593314
>50	0.37400385511666046
>100	2.468425443769959
>500	3.2710952559049455
>1k	3.147386288443281
>5k	0.0
>10k+	87.65499582841854
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCCGAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGA	30468	87.65499582841854	No Hit
TCCCGAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGA	1094	3.147386288443281	No Hit
TCCCGAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGC	624	1.79521850455997	No Hit
TCCCGAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGC	513	1.4758767513449753	No Hit
TCCCGAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGC	376	1.0817342271066486	No Hit
TCCCGAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGG	366	1.0529646997899824	No Hit
TCCCGAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGG	116	0.33372651687332777	No Hit
TCCCGAACTCCTACGGGAGGCAGCAGTAGGGAATCTTTCACAATGGGCGA	77	0.22152536033832965	No Hit
TCCCGAACTCCTACGGGAGGCATCAGTAGGGAATCTTCCACAATGGGCGA	53	0.1524784947783308	No Hit
TCCCGAACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGA	49	0.1409706838516643	No Hit
TCCCGAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC	39	0.11220115653499813	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0	0.0
260-269	0.0	0.0	0.0	0.0	0.0	0.0
270-279	0.0	0.0	0.0	0.0	0.0	0.0
280-289	0.0	0.0	0.0	0.0	0.0	0.0
290-299	0.0	0.0	0.0	0.0	0.0	0.0
300-309	0.0	0.0	0.0	0.0	0.0	0.0
310-319	0.0	0.0	0.0	0.0	0.0	0.0
320-329	0.0	0.0	0.0	0.0	0.0	0.0
330-339	0.0	0.0	0.0	0.0	0.0	0.0
340-349	0.0	0.0	0.0	0.0	0.0	0.0
350-359	0.0	0.0	0.0	0.0	0.0	0.0
360-369	0.0	0.0	0.0	0.0	0.0	0.0
370-379	0.0	0.0	0.0	0.0	0.0	0.0
380-389	0.0	0.0	0.0	0.0	0.0	0.0
390-399	0.0	0.0	0.0	0.0	0.0	0.0
400-409	0.0	0.0	0.0	0.0	0.0	0.0
410-419	0.0	0.0	0.0	0.0	0.0	0.0
420-429	0.0	0.0	0.0	0.0	0.0	0.0
430-439	0.0	0.0	0.0	0.0	0.0	0.0
440-449	0.0	0.0	0.0	0.0	0.0	0.0
450-459	0.0	0.0	0.0	0.0	0.0	0.0
460-469	0.0	0.0	0.0	0.0	0.0	0.0
470-479	0.0	0.0	0.0	0.0	0.0	0.0
480-489	0.0	0.0	0.0	0.0	0.0	0.0
490-499	0.0	0.0	0.0	0.0	0.0	0.0
500-509	0.0	0.0	0.0	0.0	0.0	0.0
510-519	0.0	0.0	0.0	0.0	0.0	0.0
520-529	0.0	0.0	0.0	0.0	0.0	0.0
530-539	0.0	0.0	0.0	0.0	0.0	0.0
540-549	0.0	0.0	0.0	0.0	0.0	0.0
550-559	0.0	0.0	0.0	0.0	0.0	0.0
560-569	0.0	0.0	0.0	0.0	0.0	0.0
570-579	0.0	0.0	0.0	0.0	0.0	0.0
580-589	0.0	0.0	0.0	0.0	0.0	0.0
590-591	0.0	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
