FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR20982543

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20982543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34473
Total Bases20.7 Mbp
Sequences flagged as poor quality0
Sequence length602
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGC1176534.12815826879007No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGC426312.366199634496562No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGA25307.339076958779335No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGCGA21766.3121863487366925No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGC20906.062715748556841No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGAATTTTGCGCAATGCTCGA17375.0387259594465235No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGG15674.5455864009514695No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGG12083.504191686247208No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTAGGGAATCTTTCACAATGGGCGA6891.9986656223711312No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGC6291.8246163664316999No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGA4481.2995677776810837No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGG2980.8644446378325066No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCTT2880.8354364285092681No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGG2850.8267339657122965No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGAGA2550.7397093377425812No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGA2480.7194035912163143No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGGATATTGGACAATGGGGGA2440.7078003074870188No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGA2370.6874945609607519No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGAGGA2130.6178748585849795No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGCGA1770.513445305021321No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGC1700.4931395584950541No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGA1440.41771821425463407No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGC1220.3539001537435094No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGC1210.35099933281118556No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGAATTTTGCGCAATGGGGGA1200.3480985118788617No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGA1190.34519769094653785No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGG1080.31328866069097555No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGA1080.31328866069097555No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGA1010.2929829141647086No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGA830.24076813738287936No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGAGGGATATTGGTCAATGGGGGA740.21466074899196474No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGG740.21466074899196474No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGA660.19145418153337393No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGA540.15664433034548778No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTTAGGAATATTCGTCAATGGGGGA460.133437762886897No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGA410.11893365822527775No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTCGGGAATATTGCACAATGGAGGA380.11023119542830623No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGC370.10733037449598237No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGA360.10442955356365852No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGA360.10442955356365852No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGT350.10152873263133468No Hit
ACAGTGACTCCTACGGGAGGCAGCAGTGAGGAATATTGGGCAATGGGCGG350.10152873263133468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)