FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR20982547

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20982547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52560
Total Bases31.6 Mbp
Sequences flagged as poor quality0
Sequence length602
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAACACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGC1994737.95091324200913No Hit
GTGAACACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGC674212.827245053272451No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGC39557.524733637747337No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGG37877.205098934550989No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGC31856.059741248097413No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGG31325.9589041095890405No Hit
GTGAACACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGA14112.6845509893455097No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGA11992.2812024353120246No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGA8821.678082191780822No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGAGA7151.3603500761035008No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGA6261.191019786910198No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGCGA5781.099695585996956No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGT5150.9798325722983257No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGG4780.9094368340943684No Hit
GTGAACACTCCTACGGGAGGCAGCAGTAGGGAATCTTTCACAATGGGCGA4090.778158295281583No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGGGGAATTTTGCGCAATGGGGGA3580.6811263318112634No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGA3430.6525875190258752No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGA2880.547945205479452No Hit
GTGAACACTCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGA2740.5213089802130898No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGGGGAATTTTGCGCAATGCTCGA2700.5136986301369862No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGC2580.4908675799086758No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGT2110.40144596651445963No Hit
GTGAACACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGCCGC1810.3443683409436834No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGGGGGATATTGGACAATGGGGGA1580.3006088280060883No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCTA1410.2682648401826484No Hit
GTGAACACTCCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGA1260.2397260273972603No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC940.17884322678843229No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGG910.17313546423135465No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGG800.15220700152207No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGC790.15030441400304415No Hit
GTGAACACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGA750.14269406392694062No Hit
GTGAACACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGC630.11986301369863014No Hit
GTGAACACTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGA540.10273972602739725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)