FastQCFastQC Report
Wed 13 Sep 2023
stdin:SRR20982552

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20982552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50299
Total Bases30.2 Mbp
Sequences flagged as poor quality0
Sequence length602
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGCTACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGA1990639.575339469969585No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGC931318.515278633770055No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGC605112.030060239766199No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGC25555.079623849380703No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTAGGGAATCTTTCACAATGGGCGA17933.5646831944969084No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGA11392.2644585379431No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGA9991.9861229845523767No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGC7661.522893099266387No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGA7011.3936658780492654No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGA6261.2445575458756635No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGG5101.0139366587804926No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGA4180.8310304379808744No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGG3800.7554822163462495No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGA3160.6282431062247759No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGC2660.5288375514423747No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGG2450.48708721843376607No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGAATTTTGCGCAATGGGGGA2340.46521799638163785No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGA2230.44334877432950953No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGA2220.4413606632338615No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTTAGGAATATTCGTCAATGGGGGA2130.42346766337302927No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGGATATTGGACAATGGGGGG1860.3697886637905326No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGG1790.3558718861209964No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGAATTTTGCGCAATGCTCGA1680.33400266406886814No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGA1670.33201455297322013No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGT1550.30815721982544386No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGACGA1490.2962285532515557No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGAGG1140.2266446649038748No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGAGA1130.22465655380822677No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGG860.17097755422573013No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCTT850.16898944313008213No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGC840.16700133203443407No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGC790.15706077655619397No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTCGGGAATATTGCACAATGGAGGA730.1451321099823058No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGGATATTGGACAATGGGGGA670.13320344340841767No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGC530.10536988806934532No Hit
AGAGCTACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGT520.1033817769736973No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)