##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21948629
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13191508
Total Bases	1.3 Gbp
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.10606808561993	37.0	36.0	37.0	35.0	38.0
2	35.85394346120246	37.0	36.0	37.0	34.0	38.0
3	36.02042647436517	37.0	36.0	37.0	35.0	38.0
4	36.015538481271435	37.0	36.0	37.0	35.0	38.0
5	36.00365523031938	37.0	36.0	37.0	35.0	38.0
6	35.993321688468065	37.0	36.0	37.0	35.0	38.0
7	35.977296606271246	37.0	36.0	37.0	35.0	38.0
8	35.98400501292195	37.0	36.0	37.0	35.0	38.0
9	35.99889398543366	37.0	36.0	37.0	35.0	38.0
10-11	35.98515988467733	37.0	36.0	37.0	34.5	38.0
12-13	35.97951955151754	37.0	36.0	37.0	35.0	38.0
14-15	35.97948775075602	37.0	36.0	37.0	34.5	38.0
16-17	35.971546353911926	37.0	36.0	37.0	34.0	38.0
18-19	35.97086591616364	37.0	36.0	37.0	34.0	38.0
20-21	35.9570614671196	37.0	36.0	37.0	34.0	38.0
22-23	35.95369327752369	37.0	36.0	37.0	34.0	38.0
24-25	35.948260615844674	37.0	36.0	37.0	34.0	38.0
26-27	35.932667933036925	37.0	36.0	37.0	34.0	38.0
28-29	35.91328114268664	37.0	36.0	37.0	34.0	38.0
30-31	35.91213870317176	37.0	36.0	37.0	34.0	38.0
32-33	35.902111343145904	37.0	36.0	37.0	34.0	38.0
34-35	35.88040218752852	37.0	36.0	37.0	34.0	38.0
36-37	35.863652396678226	37.0	36.0	37.0	34.0	38.0
38-39	35.84987845968786	37.0	36.0	37.0	34.0	38.0
40-41	35.82524306546303	37.0	36.0	37.0	34.0	38.0
42-43	35.80580957840453	37.0	36.0	37.0	34.0	38.0
44-45	35.77898952113739	37.0	36.0	37.0	34.0	38.0
46-47	35.75433396242492	37.0	36.0	37.0	34.0	38.0
48-49	35.74412697926575	37.0	36.0	37.0	34.0	38.0
50-51	35.71460601775021	37.0	36.0	37.0	34.0	38.0
52-53	35.694785160271294	37.0	36.0	37.0	34.0	38.0
54-55	35.669786653656274	37.0	36.0	37.0	34.0	38.0
56-57	35.662117477395306	37.0	36.0	37.0	34.0	38.0
58-59	35.62369984538538	37.0	36.0	37.0	33.5	38.0
60-61	35.60739530309954	37.0	36.0	37.0	33.0	38.0
62-63	35.57005101312147	37.0	36.0	37.0	33.0	38.0
64-65	35.536535133056816	37.0	36.0	37.0	33.0	38.0
66-67	35.501468672118456	37.0	36.0	37.0	33.0	38.0
68-69	35.46258756011822	37.0	36.0	37.0	33.0	38.0
70-71	35.439865859157266	37.0	36.0	37.0	33.0	38.0
72-73	35.39276074426063	37.0	36.0	37.0	33.0	38.0
74-75	35.357970635351165	37.0	36.0	37.0	32.0	38.0
76-77	35.329706505124356	37.0	36.0	37.0	32.0	38.0
78-79	35.278237901231606	37.0	36.0	37.0	32.0	38.0
80-81	35.22729425627457	37.0	36.0	37.0	32.0	38.0
82-83	35.19713636985249	37.0	36.0	37.0	32.0	38.0
84-85	35.15264467868268	37.0	36.0	37.0	32.0	38.0
86-87	35.11822837085798	37.0	36.0	37.0	31.0	38.0
88-89	35.06536095039324	37.0	36.0	37.0	31.0	38.0
90-91	35.02624654436779	37.0	36.0	37.0	31.0	38.0
92-93	34.97461029474417	37.0	36.0	37.0	31.0	38.0
94-95	34.93086006542997	37.0	35.5	37.0	31.0	38.0
96-97	34.88328184313727	37.0	35.0	37.0	30.5	38.0
98-99	34.829607691554294	37.0	35.0	37.0	30.0	38.0
100	34.77905975571557	37.0	35.0	37.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	2.0
25	135.0
26	4169.0
27	33700.0
28	108849.0
29	188658.0
30	256678.0
31	345864.0
32	476862.0
33	707040.0
34	1164011.0
35	2278785.0
36	4834565.0
37	2792190.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	28.634262284493932	28.84921117434034	19.779505117989544	22.73702142317618
2	24.193693397297718	27.884772536998803	30.203029100236307	17.718504965467176
3	19.838361163863905	26.4903830555233	30.219259238595008	23.45199654201779
4	21.5416842411042	27.91157007978163	27.27939065040934	23.26735502870483
5	22.764107030068132	27.31577011513771	27.061242732824788	22.858880121969378
6	23.120381687976842	26.972026245975822	27.060044992581588	22.847547073465748
7	23.679567188224425	27.37141955263947	26.644542837710443	22.304470421425663
8	22.2526567849559	26.843314653639293	27.825090201969328	23.07893835943548
9	22.520624632149712	26.40851220345695	28.099107395454716	22.971755768938625
10-11	23.201452783108646	26.698532116267526	27.161064527270117	22.938950573353704
12-13	23.18315313154493	26.809345830666214	27.01588021627247	22.99162082151639
14-15	23.048248160862276	26.929301790212307	27.05815362428617	22.964296424639244
16-17	23.2437640942946	26.888715073363862	26.92422655544764	22.94329427689389
18-19	23.15206494966307	26.812105181606228	26.94375806010958	23.092071808621125
20-21	23.090040198588365	26.843371508397674	27.029275197346657	23.037313095667304
22-23	23.230945241438658	26.887248220597677	26.91646398577024	22.965342552193427
24-25	23.201793911658925	26.806082367535232	26.955216189081643	23.036907531724196
26-27	23.09319374251981	26.828634755025732	27.07135908949909	23.006812412955366
28-29	23.27413590622088	26.846858600244943	26.856395038383784	23.02261045515039
30-31	23.21429816818517	26.811737520835372	26.867258087551477	23.10670622342798
32-33	23.128231434950425	26.834399827525406	26.98895759302121	23.048411144502964
34-35	23.26946244508209	26.854026090117976	26.868008570362083	23.008502894437846
36-37	23.242354096286792	26.807636397597605	26.88681991475122	23.063189591364385
38-39	23.127981274013553	26.824484357664037	26.994900810430465	23.05263355789194
40-41	23.282747506956746	26.846127069020465	26.84275747700718	23.02836794701561
42-43	23.216344939486827	26.798569958794705	26.86164083742359	23.12344426429488
44-45	23.13053973814063	26.838209096336822	26.98683880569227	23.04441235983028
46-47	23.29625240723047	26.853760767912206	26.860738741923974	22.989248082933354
48-49	23.24634530032503	26.798524474988	26.87016147054605	23.084968754140924
50-51	23.147277779007524	26.823597423433316	26.96944883026262	23.059675967296535
52-53	23.29724926066072	26.828937980403754	26.85669447344458	23.01711828549094
54-55	23.249779327731144	26.77669982840476	26.872913240851616	23.10060760301248
56-57	23.137468438028467	26.818560091840904	26.999445400783596	23.044526069347036
58-59	23.30308255887045	26.816323804677978	26.859768420714296	23.020825215737275
60-61	23.260953182911308	26.783651270195946	26.863319947954395	23.092075598938347
62-63	23.15056777435908	26.821577184352236	26.9905154134008	23.03733962788788
64-65	23.285779760736983	26.833569748053065	26.847631824958906	23.033018666251046
66-67	23.255108513749907	26.775316362617524	26.880050408186847	23.089524715445727
68-69	23.139818434708147	26.809053976239866	26.996261534314346	23.054866054737637
70-71	23.3159772180709	26.7991233451096	26.861663579326944	23.023235857492562
72-73	23.26638091717793	26.75353340952376	26.8792051674456	23.100880505852707
74-75	23.16428493239742	26.78842327958259	26.974493742489486	23.072798045530504
76-77	23.306099651381786	26.800753181516473	26.8562775385498	23.036869628551944
78-79	23.27077010452482	26.727133850049594	26.889306362851013	23.11278968257458
80-81	23.16458815777544	26.783806673202182	26.977961882750627	23.073643286271743
82-83	23.339170169172473	26.781308854150716	26.872083161379273	23.00743781529754
84-85	23.284862503968462	26.76169396250982	26.843625459651772	23.10981807386995
86-87	23.197109079568463	26.755614293680452	26.973470356838657	23.07380626991243
88-89	23.33026292369303	26.773474268446034	26.845713924442904	23.050548883418028
90-91	23.30019054682755	26.720125553500022	26.876442026188364	23.103241873484063
92-93	23.20964365863251	26.747976804471485	26.98482235692841	23.057557179967596
94-95	23.34398766236582	26.776354909537257	26.833656925349246	23.046000502747678
96-97	23.300444498081642	26.775638539581674	26.81317405106376	23.11074291127292
98-99	23.233143625429328	26.781782643803876	26.925481150449215	23.05959258031758
100	23.484562947617512	26.760602351148936	26.68115730210678	23.073677399126773
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	4.0
3	5.5
4	5.5
5	11.0
6	16.5
7	14.5
8	27.0
9	51.0
10	71.5
11	128.5
12	220.0
13	383.5
14	715.5
15	1261.5
16	1962.5
17	3063.0
18	4665.5
19	7068.5
20	10838.0
21	15664.5
22	22156.5
23	31072.5
24	43363.0
25	57917.0
26	73878.5
27	92304.0
28	110836.5
29	130734.0
30	153732.0
31	178262.5
32	202815.5
33	227382.5
34	254107.5
35	281624.0
36	308930.5
37	336192.0
38	363401.0
39	391129.5
40	418819.5
41	443706.5
42	466054.5
43	484109.5
44	495985.0
45	502768.5
46	502742.0
47	496624.5
48	485948.0
49	473335.5
50	456479.5
51	436185.5
52	414329.0
53	390247.5
54	366718.5
55	342589.5
56	318179.5
57	295094.5
58	272555.5
59	250964.5
60	230032.5
61	209656.5
62	189661.0
63	168992.0
64	148531.0
65	128387.0
66	109179.0
67	90997.0
68	74356.5
69	59507.0
70	46411.0
71	35370.5
72	26081.5
73	18595.0
74	12802.0
75	8686.5
76	5686.5
77	3665.0
78	2297.5
79	1352.0
80	791.5
81	439.0
82	248.0
83	149.5
84	82.5
85	36.5
86	19.0
87	11.5
88	7.0
89	5.0
90	3.5
91	2.0
92	1.0
93	1.5
94	1.0
95	1.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.3191508E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.96599565129475
#Duplication Level	Percentage of total
1	83.44770201295498
2	10.442211776125866
3	2.0027759956374425
4	0.8654017736042284
5	0.5838927851831824
6	0.4446963390061686
7	0.41269841312372757
8	0.312765192859491
9	0.2861727908791303
>10	1.1744678233794184
>50	0.005321663541694613
>100	0.0033538518121277072
>500	0.0082641053492209
>1k	0.010275476543330506
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	1.5161268901174908E-5	0.0	0.0
2	0.0	0.0	2.2741903351762363E-5	1.5161268901174908E-5	7.580634450587454E-6	1.5161268901174908E-5
3	0.0	2.2741903351762363E-5	3.0322537802349816E-5	1.5161268901174908E-5	1.5161268901174908E-5	3.0322537802349816E-5
4	7.580634450587454E-6	3.0322537802349816E-5	4.5483806703524725E-5	2.2741903351762363E-5	3.790317225293727E-5	3.0322537802349816E-5
5	7.580634450587454E-6	3.790317225293727E-5	4.5483806703524725E-5	3.0322537802349816E-5	3.790317225293727E-5	3.0322537802349816E-5
6	1.5161268901174908E-5	3.790317225293727E-5	6.822571005528709E-5	3.0322537802349816E-5	3.790317225293727E-5	3.790317225293727E-5
7	1.5161268901174908E-5	5.3064441154112175E-5	7.580634450587454E-5	3.0322537802349816E-5	4.5483806703524725E-5	4.5483806703524725E-5
8	1.5161268901174908E-5	1.0612888230822435E-4	9.85482478576369E-5	3.0322537802349816E-5	5.3064441154112175E-5	6.064507560469963E-5
9	3.790317225293727E-5	1.137095167588118E-4	9.85482478576369E-5	4.5483806703524725E-5	7.580634450587454E-5	6.064507560469963E-5
10-11	4.1693489478231E-5	1.1749983398410554E-4	1.0233856508293062E-4	5.68547583794059E-5	8.338697895646199E-5	7.580634450587454E-5
12-13	4.5483806703524725E-5	1.25080468434693E-4	1.2129015120939926E-4	7.201602728058081E-5	8.717729618175571E-5	9.096761340704945E-5
14-15	4.5483806703524725E-5	1.2887078565998673E-4	1.4403205456116163E-4	7.959666173116826E-5	9.85482478576369E-5	1.1749983398410554E-4
16-17	4.927412392881845E-5	1.3266110288528044E-4	1.6298364068763024E-4	1.0233856508293062E-4	1.1749983398410554E-4	1.3266110288528044E-4
18-19	6.064507560469963E-5	1.4403205456116163E-4	1.705642751382177E-4	1.0991919953351807E-4	1.4782237178645534E-4	1.705642751382177E-4
20-21	6.443539282999336E-5	1.4403205456116163E-4	1.7435459236351142E-4	1.25080468434693E-4	1.5919332346233652E-4	1.7814490958880516E-4
22-23	6.822571005528709E-5	1.5161268901174907E-4	1.933061784899801E-4	1.4403205456116163E-4	1.7435459236351142E-4	1.970964957152738E-4
24-25	7.201602728058081E-5	1.554030062370428E-4	2.0467713016586125E-4	1.554030062370428E-4	2.0467713016586125E-4	2.160480818417424E-4
26-27	8.338697895646199E-5	1.7435459236351142E-4	2.160480818417424E-4	1.6298364068763024E-4	2.236287162923299E-4	2.4637061964409226E-4
28-29	8.338697895646199E-5	1.7435459236351142E-4	2.236287162923299E-4	1.6677395791292397E-4	2.3499966796821108E-4	2.6153188854526716E-4
30-31	8.717729618175571E-5	1.819352268140989E-4	2.50160936869386E-4	1.6677395791292397E-4	2.691125229958546E-4	3.070156952487919E-4
32-33	9.096761340704945E-5	1.933061784899801E-4	2.6153188854526716E-4	1.7435459236351142E-4	2.9185442634761696E-4	3.1459632969937934E-4
34-35	9.096761340704945E-5	2.0467713016586125E-4	2.9185442634761696E-4	1.7435459236351142E-4	2.9564474357291067E-4	3.2975759860055424E-4
36-37	9.096761340704945E-5	2.122577646164487E-4	3.259672813752605E-4	1.7814490958880516E-4	2.9564474357291067E-4	3.373382330511417E-4
38-39	9.096761340704945E-5	2.160480818417424E-4	3.5249950195231656E-4	1.8951586126468635E-4	2.994350607982044E-4	3.600801364029041E-4
40-41	1.0233856508293062E-4	2.274190335176236E-4	3.714510880787852E-4	2.08467447391155E-4	3.1838664692467305E-4	3.7903172252937265E-4
42-43	1.0612888230822435E-4	2.387899851935048E-4	3.866123569799601E-4	2.160480818417424E-4	3.2596728137526047E-4	4.4346711535936606E-4
44-45	1.0612888230822435E-4	2.4258030241879852E-4	3.941929914305476E-4	2.274190335176236E-4	3.411285502764354E-4	4.889509220628908E-4
46-47	1.137095167588118E-4	2.653222057705609E-4	4.1314457755701626E-4	2.3499966796821108E-4	3.638704536281978E-4	5.116928254146531E-4
48-49	1.137095167588118E-4	2.7669315744644206E-4	4.245155292328974E-4	2.3499966796821108E-4	3.7524140530407893E-4	5.230637770905343E-4
50-51	1.2129015120939926E-4	2.9185442634761696E-4	4.510477498099535E-4	2.387899851935048E-4	3.904026742052539E-4	5.571766321181778E-4
52-53	1.2129015120939926E-4	3.2217696414996676E-4	4.6999933593642215E-4	2.50160936869386E-4	4.1314457755701626E-4	5.799185354699402E-4
54-55	1.2129015120939926E-4	3.411285502764354E-4	5.00321873738772E-4	2.653222057705609E-4	4.283058464581911E-4	6.026604388217026E-4
56-57	1.2129015120939926E-4	3.4870918472702285E-4	5.154831426399469E-4	2.7669315744644206E-4	4.548380670352472E-4	6.367732938493461E-4
58-59	1.2887078565998673E-4	3.4870918472702285E-4	5.382250459917092E-4	2.9564474357291067E-4	4.6999933593642215E-4	6.898377350034583E-4
60-61	1.2887078565998673E-4	3.5249950195231656E-4	5.533863148928841E-4	3.108060124740856E-4	4.775799703870096E-4	7.277409072563956E-4
62-63	1.2887078565998673E-4	3.7903172252937265E-4	5.685475837940591E-4	3.1838664692467305E-4	4.965315565134782E-4	7.808053484105078E-4
64-65	1.4024173733586791E-4	4.093542603317225E-4	5.912894871458213E-4	3.1838664692467305E-4	5.116928254146531E-4	8.3386978956462E-4
66-67	1.4782237178645534E-4	4.245155292328974E-4	6.1024107327229E-4	3.2596728137526047E-4	5.192734598652406E-4	9.02095499619907E-4
68-69	1.5161268901174907E-4	4.358864809087786E-4	6.443539282999335E-4	3.411285502764354E-4	5.42015363217003E-4	9.362083546475505E-4
70-71	1.554030062370428E-4	4.472574325846598E-4	6.595151972011085E-4	3.5249950195231656E-4	5.647572665687653E-4	0.0010233856508293062
72-73	1.5919332346233652E-4	4.472574325846598E-4	6.784667833275771E-4	3.600801364029041E-4	5.912894871458213E-4	0.0011105629470110619
74-75	1.6677395791292397E-4	4.586283842605409E-4	7.125796383552206E-4	3.676607708534915E-4	6.25402342173465E-4	0.001163627388165174
76-77	1.7435459236351142E-4	4.8516060483759705E-4	7.429021761575704E-4	3.790317225293727E-4	6.595151972011085E-4	0.001277336904923986
78-79	1.7435459236351142E-4	4.927412392881845E-4	7.618537622840391E-4	3.790317225293727E-4	6.974183694540457E-4	0.0013607238838804479
80-81	1.7814490958880516E-4	4.927412392881845E-4	7.808053484105078E-4	3.866123569799601E-4	7.580634450587454E-4	0.0014099980078092662
82-83	1.8951586126468635E-4	4.965315565134782E-4	7.921763000863889E-4	3.941929914305476E-4	7.921763000863889E-4	0.0015312881590186656
84-85	2.008868129405675E-4	5.154831426399469E-4	8.0354725176227E-4	3.979833086558413E-4	8.528213756910886E-4	0.0016033041862992465
86-87	2.0467713016586125E-4	5.306444115411217E-4	8.224988378887387E-4	4.1314457755701626E-4	9.02095499619907E-4	0.0016601589446786523
88-89	2.0467713016586125E-4	5.306444115411217E-4	8.262891551140324E-4	4.358864809087786E-4	9.437889890981379E-4	0.001777658778662758
>>END_MODULE
