##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21948630
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	104947524
Total Bases	10.4 Gbp
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.762022932527685	37.0	36.0	37.0	34.0	38.0
2	35.47876888453319	37.0	36.0	37.0	33.0	37.0
3	35.64815182300061	37.0	36.0	37.0	34.0	38.0
4	35.63977699940782	37.0	36.0	37.0	34.0	37.0
5	35.660813722484775	37.0	36.0	37.0	34.0	38.0
6	35.63511885235139	37.0	36.0	37.0	34.0	37.0
7	35.625760346666205	37.0	36.0	37.0	34.0	37.0
8	35.63195936856976	37.0	36.0	37.0	34.0	37.0
9	35.6115370859059	37.0	36.0	37.0	34.0	38.0
10-11	35.61593011951383	37.0	36.0	37.0	34.0	37.5
12-13	35.61257574785661	37.0	36.0	37.0	34.0	37.5
14-15	35.61917145372577	37.0	36.0	37.0	34.0	38.0
16-17	35.60484516052041	37.0	36.0	37.0	34.0	38.0
18-19	35.605722680031974	37.0	36.0	37.0	34.0	38.0
20-21	35.57939145377074	37.0	36.0	37.0	34.0	37.0
22-23	35.57333651816312	37.0	36.0	37.0	33.5	37.0
24-25	35.56923357238995	37.0	36.0	37.0	33.0	37.5
26-27	35.547761755675154	37.0	36.0	37.0	33.0	37.0
28-29	35.536036362325234	37.0	36.0	37.0	33.0	37.0
30-31	35.534075229826286	37.0	36.0	37.0	33.0	37.0
32-33	35.517971510218764	37.0	36.0	37.0	33.0	37.0
34-35	35.49478012935303	37.0	36.0	37.0	33.0	37.0
36-37	35.47193631743042	37.0	36.0	37.0	33.0	37.0
38-39	35.45954734482349	37.0	36.0	37.0	33.0	37.0
40-41	35.43825946289118	37.0	36.0	37.0	33.0	37.0
42-43	35.417089025368526	37.0	36.0	37.0	33.0	37.0
44-45	35.387218368296146	37.0	36.0	37.0	33.0	37.0
46-47	35.36712206759637	37.0	36.0	37.0	33.0	37.0
48-49	35.346257878365954	37.0	36.0	37.0	33.0	37.0
50-51	35.31885142902466	37.0	36.0	37.0	32.0	37.0
52-53	35.29975502328192	37.0	36.0	37.0	32.0	37.0
54-55	35.278116847235005	37.0	36.0	37.0	32.0	37.0
56-57	35.264056868078185	37.0	36.0	37.0	32.0	37.0
58-59	35.23509454591802	37.0	36.0	37.0	32.0	37.0
60-61	35.208307096411346	37.0	36.0	37.0	32.0	37.0
62-63	35.165559951657364	37.0	36.0	37.0	32.0	37.0
64-65	35.11858507972041	37.0	35.5	37.0	32.0	37.0
66-67	35.09459817270201	37.0	35.0	37.0	31.5	37.0
68-69	35.059884919247835	37.0	35.0	37.0	31.0	37.0
70-71	35.02677709671359	37.0	35.0	37.0	31.0	37.0
72-73	34.98471140681699	37.0	35.0	37.0	31.0	37.0
74-75	34.94924706846824	37.0	35.0	37.0	31.0	37.0
76-77	34.916951575722734	37.0	35.0	37.0	31.0	37.0
78-79	34.86950242389711	37.0	35.0	37.0	31.0	37.0
80-81	34.81391681999091	37.0	35.0	37.0	30.5	37.0
82-83	34.77259373456014	37.0	35.0	37.0	30.0	37.0
84-85	34.73480896509764	37.0	35.0	37.0	30.0	37.0
86-87	34.68094648902817	37.0	35.0	37.0	30.0	37.0
88-89	34.6363330496487	37.0	35.0	37.0	30.0	37.0
90-91	34.584927110810156	37.0	35.0	37.0	29.0	37.0
92-93	34.522750894056344	37.0	35.0	37.0	29.0	37.0
94-95	34.48237563946721	37.0	35.0	37.0	29.0	37.0
96-97	34.43247026485351	37.0	35.0	37.0	29.0	37.0
98-99	34.373744477287524	37.0	35.0	37.0	28.0	37.0
100	34.333841644515594	37.0	35.0	37.0	28.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	204.0
25	7916.0
26	95780.0
27	503428.0
28	1361507.0
29	2181487.0
30	2856648.0
31	3698929.0
32	4949871.0
33	7034057.0
34	1.0911055E7
35	1.9541649E7
36	3.7183928E7
37	1.4621063E7
38	2.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	26.16415228624164	28.97166111322455	23.081433536249985	21.78275306428382
2	23.58331293266147	29.474175112506707	27.418766925840004	19.523745028991822
3	20.653716423076357	27.486674197287396	29.26688770665995	22.592721672976296
4	21.933809510360625	27.998229858190843	27.7323712753814	22.335589356067135
5	22.298070605267448	27.85477530656178	27.831302623204333	22.015851464966435
6	22.198552297432002	28.022462921564497	27.59394780957386	22.185036971429646
7	22.53984953470651	28.1615934073871	27.445676564984993	21.852880492921393
8	21.70071968539248	27.96112464739997	28.083519150008723	22.254636517198826
9	21.729177717427618	27.587549373723196	28.46439426241252	22.21887864643667
10-11	22.062922608826867	27.961393353119984	27.975295062702006	22.00038897535115
12-13	22.04993087783567	27.88393321218326	27.947288208533628	22.11884770144744
14-15	21.890294905861715	27.984664983616003	28.039578141929294	22.08546196859299
16-17	22.077707616999138	27.924299100186488	27.94267876200681	22.055314520807563
18-19	22.047721202074285	27.76614434467268	27.941938868419612	22.244195584833427
20-21	22.009286279112217	27.77302111481925	27.992591802356387	22.22510080371215
22-23	22.195500772366962	27.85322453152873	27.805704115515866	22.145570580588448
24-25	22.123823521553497	27.75059133362689	27.863846030326545	22.261739114493068
26-27	22.05458034436334	27.78162112714541	27.93857242406214	22.225226104429105
28-29	22.235432633932366	27.820569163689846	27.767464528272246	22.17653367410555
30-31	22.199717641742552	27.732326014666146	27.76335342604176	22.304602917549538
32-33	22.10748535620526	27.77057560690998	27.858533375213312	22.263405661671445
34-35	22.244869731276363	27.83969586552609	27.735337519730336	22.180096883467208
36-37	22.21188038700179	27.737803990497195	27.758759701658136	22.291555920842878
38-39	22.137144941123147	27.750869091489953	27.847175317828366	22.264810649558537
40-41	22.281709094918714	27.797990736780033	27.70357259691043	22.21672757139082
42-43	22.231253402414715	27.7094898398937	27.728982438880596	22.33027431881099
44-45	22.13771474970672	27.757092201622598	27.825774622372222	22.279418426298463
46-47	22.273833730465142	27.808145335567897	27.715417564305756	22.202603369661205
48-49	22.247123238467257	27.69950532610946	27.741715469151995	22.311655966271296
50-51	22.166816913184153	27.729102022454576	27.815719597157912	22.28836146720336
52-53	22.305298026850068	27.776848265615108	27.689008651600012	22.228845055934812
54-55	22.255485989359787	27.69455332743248	27.71908415866962	22.330876524538112
56-57	22.15474040149818	27.73453187899888	27.83067611961955	22.280051599883386
58-59	22.30368960395864	27.765348708941435	27.716654372903548	22.21430731419638
60-61	22.26664204102614	27.67396160770787	27.726180085963726	22.333216265302266
62-63	22.18483353642531	27.7028570011809	27.806653161250377	22.30565630114342
64-65	22.321549958625038	27.755071668008096	27.684818462224985	22.238559911141877
66-67	22.267681608191157	27.671475126940585	27.72845407958362	22.332389185284637
68-69	22.179284572735607	27.70100512328428	27.823117580172735	22.296592723807375
70-71	22.328837410208934	27.743420130616897	27.695862076746085	22.23188038242808
72-73	22.29642216237517	27.643686477062573	27.712598059959948	22.34729330060231
74-75	22.211832744143635	27.674595257721375	27.80234767615861	22.31122432197638
76-77	22.336756129663428	27.736185086176974	27.68428962649943	22.242769157660167
78-79	22.29579994664762	27.63923139339619	27.723436333762386	22.341532326193803
80-81	22.223749652254778	27.66380272106277	27.797874488206126	22.31457313847633
82-83	22.37117714182566	27.70681183483662	27.668229695442836	22.25378132789488
84-85	22.322430398643803	27.62235724589367	27.69602168032092	22.35919067514161
86-87	22.250119974245415	27.64974474290599	27.77503069057637	22.32510459227223
88-89	22.378122994116563	27.696528123903143	27.667271597565275	22.258077284415016
90-91	22.348729256347198	27.598991758967085	27.69550809030997	22.356770894375746
92-93	22.27358312903123	27.618208505805242	27.767818038279778	22.34039032688375
94-95	22.410858401957153	27.670031071909808	27.644870402087808	22.27424012404523
96-97	22.37799483482812	27.60438302479628	27.652141655099932	22.365480485275672
98-99	22.30150136748343	27.61658817219928	27.741982269157678	22.33992819115961
100	22.514882771317215	27.628315461734953	27.55250424011909	22.30429752682874
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	209.0
1	185.0
2	205.0
3	284.5
4	446.5
5	624.5
6	758.0
7	971.0
8	1371.5
9	2201.0
10	3743.5
11	6508.5
12	11431.0
13	19451.0
14	32029.5
15	51536.0
16	80567.5
17	120299.0
18	170972.0
19	234535.0
20	313468.0
21	408717.0
22	519687.5
23	644492.0
24	782060.0
25	927665.0
26	1079805.5
27	1237615.5
28	1394126.0
29	1542477.0
30	1684534.0
31	1824800.0
32	1960100.5
33	2094723.0
34	2228704.0
35	2362504.0
36	2507853.5
37	2667898.0
38	2831616.0
39	3002936.5
40	3178494.5
41	3348032.0
42	3502840.5
43	3630139.0
44	3731924.5
45	3796399.0
46	3818376.0
47	3798519.5
48	3728823.0
49	3615074.5
50	3461783.5
51	3273218.5
52	3061519.5
53	2832832.5
54	2602785.5
55	2386202.0
56	2180558.0
57	1989883.0
58	1816679.5
59	1658181.5
60	1516210.5
61	1382131.5
62	1252384.0
63	1126959.5
64	1002795.5
65	879129.0
66	758104.5
67	641841.0
68	530897.0
69	429025.0
70	339079.5
71	262584.0
72	198004.5
73	145594.0
74	104628.5
75	73239.5
76	49948.0
77	33133.0
78	21519.0
79	13577.5
80	8337.0
81	5071.5
82	3071.5
83	1786.0
84	962.5
85	516.5
86	271.5
87	153.5
88	92.0
89	51.0
90	39.5
91	28.0
92	23.5
93	18.0
94	11.0
95	8.0
96	5.5
97	4.0
98	4.5
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.04947524E8
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.21702711034244
#Duplication Level	Percentage of total
1	70.28570518733605
2	17.31787198667368
3	6.24805230296476
4	2.6319767418439355
5	1.2830697207034039
6	0.6784212247561581
7	0.424092627815554
8	0.241739134090419
9	0.19870309876512623
>10	0.6685726000359572
>50	0.012376456515575583
>100	0.0065232077358051786
>500	0.001275100300613933
>1k	0.0016206104629615586
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.9057143263332254E-6	5.7171429789996765E-6	0.0	3.811428652666451E-6	1.0481428794832739E-5	7.622857305332902E-6
2	3.811428652666451E-6	8.575714468499513E-6	4.764285815833063E-6	9.528571631666126E-6	2.7632857731831767E-5	9.528571631666126E-6
3	5.7171429789996765E-6	1.9057143263332252E-5	1.0481428794832739E-5	2.0010000426498866E-5	4.383142950566418E-5	1.1434285957999353E-5
4	7.622857305332902E-6	2.191571475283209E-5	1.3340000284332578E-5	2.7632857731831767E-5	6.670000142166289E-5	1.429285744749919E-5
5	1.0481428794832739E-5	2.7632857731831767E-5	1.6198571773832416E-5	3.239714354766483E-5	7.622857305332901E-5	1.9057143263332252E-5
6	1.1434285957999353E-5	2.9538572058164992E-5	2.0962857589665477E-5	3.906714368983112E-5	9.242714482716143E-5	2.0010000426498866E-5
7	1.429285744749919E-5	3.906714368983112E-5	2.6680000568665156E-5	4.1925715179330954E-5	1.0862571660099385E-4	2.3821429079165317E-5
8	1.6198571773832416E-5	4.573714383199741E-5	2.9538572058164992E-5	4.8595715321497244E-5	1.3149428851699256E-4	2.7632857731831767E-5
9	1.810428610016564E-5	5.1454286810997084E-5	3.239714354766483E-5	5.5265715463663534E-5	1.4483428880132513E-4	3.620857220033128E-5
10-11	1.9057143263332252E-5	6.288857276899644E-5	3.620857220033128E-5	6.193571560582982E-5	1.6770286071732384E-4	4.0972858016164344E-5
12-13	2.191571475283209E-5	7.051143007432933E-5	4.192571517933096E-5	7.098785865591265E-5	1.9438286128598898E-4	4.764285815833064E-5
14-15	2.5250714823915236E-5	7.861071596124554E-5	4.716642957674733E-5	8.004000170599547E-5	2.253507190889039E-4	5.3836429718913615E-5
16-17	2.620357198708185E-5	8.718643042974506E-5	5.7647858371580065E-5	9.338000199032805E-5	2.529835768207356E-4	6.00300012794966E-5
18-19	3.00150006397483E-5	9.099785908241151E-5	6.384142993216306E-5	1.0386143078516079E-4	2.8014000597098414E-4	7.289357298224586E-5
20-21	3.2873572129248134E-5	1.0052643071407764E-4	7.098785865591265E-5	1.1243714525366029E-4	3.072964351212326E-4	7.908714454282886E-5
22-23	3.7637857945081195E-5	1.1720143106949335E-4	7.47992873085791E-5	1.2196571688532641E-4	3.2778286412931475E-4	8.575714468499514E-5
24-25	3.906714368983112E-5	1.3006500277224262E-4	7.956357312441216E-5	1.3292357426174248E-4	3.46840007392647E-4	9.528571631666127E-5
26-27	4.2878572342497565E-5	1.4102286014865866E-4	9.00450019192449E-5	1.4149928873024198E-4	3.682792935638958E-4	1.0814928801941054E-4
28-29	4.7642858158330633E-5	1.510278603619081E-4	1.0386143078516079E-4	1.5150428894349143E-4	4.0067643711156064E-4	1.1910714539582659E-4
30-31	5.3360001137330306E-5	1.6103286057515755E-4	1.0814928801941055E-4	1.6246214631990746E-4	4.297385805881423E-4	1.3292357426174245E-4
32-33	5.8600715534746676E-5	1.7199071795157358E-4	1.1386643099841022E-4	1.7580214660424005E-4	4.7071143860430664E-4	1.4674000312765836E-4
34-35	6.479428709532966E-5	1.8628357539907277E-4	1.1910714539582659E-4	1.8628357539907277E-4	5.040614393151382E-4	1.6246214631990746E-4
36-37	7.241714440066256E-5	1.962885756123222E-4	1.2387143121165965E-4	2.024821471729052E-4	5.340764399548864E-4	1.791371466753232E-4
38-39	7.765785879807893E-5	2.034350043360718E-4	1.3482928858807568E-4	2.2011000469148752E-4	5.674264406657179E-4	1.9867071852023874E-4
40-41	8.2422144613912E-5	2.172514332019877E-4	1.4483428880132513E-4	2.344028621389867E-4	5.941064412343831E-4	2.1153429022298801E-4
42-43	8.528071610341184E-5	2.2582714767048723E-4	1.510278603619081E-4	2.477428624233193E-4	6.23645013292548E-4	2.3059143348632026E-4
44-45	8.861571617449498E-5	2.324971478126535E-4	1.5722143192249108E-4	2.6013000554448525E-4	6.517542996059631E-4	2.563185768918188E-4
46-47	8.95685733376616E-5	2.396435765364031E-4	1.6389143206465737E-4	2.687057200129848E-4	6.865335860615444E-4	2.810928631341508E-4
48-49	9.195071624557813E-5	2.477428624233193E-4	1.7008500362524035E-4	2.7871072022623417E-4	7.217893010987091E-4	3.020557207238162E-4
50-51	9.719143064299449E-5	2.6013000554448525E-4	1.7723143234898997E-4	2.915742919289835E-4	7.518043017384574E-4	3.2540072122139824E-4
52-53	9.909714496932772E-5	2.7108786292090133E-4	1.8485428965432285E-4	3.0253214930539953E-4	7.808664452150392E-4	3.4922215030056355E-4
54-55	9.909714496932772E-5	2.839514346236506E-4	1.9390643270440568E-4	3.1301357810023224E-4	8.108814458547874E-4	3.711378650533957E-4
56-57	1.0243214504041087E-4	2.906214347658169E-4	2.029585757544885E-4	3.306414356188146E-4	8.466135894735354E-4	3.9972357994839404E-4
58-59	1.0433785936674408E-4	3.015792921422329E-4	2.105814330598214E-4	3.458871502294804E-4	8.856807331633664E-4	4.373614378934752E-4
60-61	1.0910214518257714E-4	3.1063143519231574E-4	2.172514332019877E-4	3.606564362585629E-4	9.209364482005312E-4	4.635650098805571E-4
62-63	1.1434285957999353E-4	3.225421497318984E-4	2.2249214759940408E-4	3.744728651244788E-4	9.742964493378614E-4	5.064435822230546E-4
64-65	1.1767785965107667E-4	3.3492929285306437E-4	2.2487429050732058E-4	3.835250081745616E-4	0.0010252743075672753	5.517042974734687E-4
66-67	1.1958357397740988E-4	3.430285787399805E-4	2.2725643341523714E-4	3.9448286555097766E-4	0.0010719643085624394	5.955357269791329E-4
68-69	1.2434785979324297E-4	3.511278646268968E-4	2.320207192310702E-4	4.05917151508977E-4	0.0011262771668629362	6.546128710954629E-4
70-71	1.276828598643261E-4	3.6399143632964606E-4	2.3583214788373662E-4	4.187807232117263E-4	0.0011724907392765167	7.175014438644593E-4
72-73	1.3244714568015918E-4	3.7161429363497894E-4	2.4297857660748624E-4	4.302150091697256E-4	0.0012158457402005978	7.775314451439559E-4
74-75	1.362585743328256E-4	3.8733643682722804E-4	2.506014339128191E-4	4.411728665461417E-4	0.001263012169777345	8.509014467077852E-4
76-77	1.3911714582232545E-4	4.0210572285631055E-4	2.558421483102355E-4	4.521307239225577E-4	0.001313990028006759	9.323707341585305E-4
78-79	1.419757173118253E-4	4.1687500888539305E-4	2.610828627076519E-4	4.6023000980947393E-4	0.0014102286014865866	0.0010224157360777756
80-81	1.4674000312765836E-4	4.302150091697256E-4	2.687057200129848E-4	4.6737643853322355E-4	0.0014578714596449175	0.0011053143092732707
82-83	1.495985746171582E-4	4.4022000938297504E-4	2.7394643441040116E-4	4.7642858158330635E-4	0.001522665746740247	0.001228709311903347
84-85	1.5293357468824134E-4	4.4974858101464116E-4	2.796635773894008E-4	4.88339296122889E-4	0.001595559319722493	0.0013344764570148411
86-87	1.5579214617774116E-4	4.583242954831407E-4	2.844278632052339E-4	4.997735820808884E-4	0.001667023606959989	0.001454060030992251
88-89	1.5960357483040764E-4	4.664235813700569E-4	2.8919214902106694E-4	5.10255010875721E-4	0.0017446814657580678	0.00159079503390666
>>END_MODULE
