FastQCFastQC Report
Thu 27 Jul 2023
1-A03_S17_L001_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1-A03_S17_L001_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190476
Total Bases28.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTGAGTCTCTGCACCTATCCTTTTTGATTTTCGCTTTCTGAACCGTT2549113.382788382788382No Hit
CCATTGAGTCTCTGCACCTATCCTTTTTTTTTATTCTTGCTTTTGAAAAA2530513.285138285138284No Hit
GGGCAATCCTGAGCCAAATCACGTTTTCCGAAAACAAACAACGGTTCAGA2388612.54016254016254No Hit
GGGCAATCCTGAGCCAACTCCTTTTTTCAAAAGCAAGAATAAAAAAAAAG2323712.199437199437199No Hit
GGGCAATCCTGAGCCAACTCCTTTTTTCAAAAGAAAAAAAATAAGGATTC38162.0034020034020035No Hit
CCATTGAGTCTCTGCACCTATCCTTTTTTTTTATTCTTGTTTTCGGAATC35841.8816018816018816No Hit
CCATTGAGTCTCTGCACCTATCCTTTATTTATTCTCGCTTTCTGAAACCC31031.6290766290766292No Hit
GGGCAATCCTGAGCCAAATCCCGTTTTATGAAAACAAACAAGGGTTTCAG29291.537726537726538No Hit
GGGCAATCCTGAGCCAAATCCTATTTTCCAAAAACAAACAAAGGCCCAGA21561.1319011319011318No Hit
CCATTGAGTCTCTGCACCTATCCTTTTTTCACCTTCTGGGCCTTTGTTTG19481.0227010227010227No Hit
GGGCAATCCTGAGCCAACTCCTTTTTTCAAAAGCAAGAATAAAAAAAAAA17800.9345009345009345No Hit
CCATTGAGTCTCTGCACCTATCCTTTTTTTTTTATTCTTGCTTTTGAAAA16250.8531258531258532No Hit
CCATTGAGTCTCTGCACCTATCCTTTTTTTTATTCTTGCTTTTGAAAAAA12930.6788256788256788No Hit
GGGCAATCCTGAGCCAACTCCTTTTTTCAAAAGCAAGAATAAAAAAAAGG12060.6331506331506331No Hit
CCATTGAGTCTCTGCACCTATCTCGTGGGCTCAGGATTGCCCCTGTCTCT10490.5507255507255507No Hit
GGGCAATCCTGAGCCCACGAGATAGGTGCAGAGACTCAATGGCTGTCTCT10000.5250005250005251No Hit
CCATTGAGTCTCTGCACCTATCCTTTTTTGATTTTCACTTTCTGAACTTT8070.42367542367542366No Hit
GGGCAATCCTGAGCCAAATCCTTCTTTCTGAAAACAAATAAAAGTTCAGA7340.38535038535038535No Hit
GGGCAATCCTGAGCCAACTCCTTTTTTCAAAAGCAAGAATAAAAAAAATG4770.25042525042525043No Hit
CCATTGAGTCTCTGCACCTATCCTTTTTTTTTATTCTTGCTATTGAAAAA4730.24832524832524833No Hit
GGGCAATCCTGAGCCAACTCCATTTTTCAAAAGCAAGAATAAAAAAAAAG3920.20580020580020583No Hit
CCATTGAGTCTCTGCACCTATCCCTTTTTCTTTCGGAATCTTTATTTGTT3810.20002520002520002No Hit
CCATTGAGTCTCTGCACCTATCCTTTTTTTCTGAACCTTGGTTTGTTTTC3810.20002520002520002No Hit
CCATTGAGTCTCTGCACCTATCCTTTTTTTTTATACTTGCTTTTGAAAAA3670.19267519267519267No Hit
GGGCAATCCTGAGCCAAATCCTGTTTTCTGAAAACAAACCAAGGTTCAGA3650.19162519162519162No Hit
GGGCAATCCTGAGCCAAATCCTTTTTTTTGGCAAACAAATAAAGATTCCG3560.1869001869001869No Hit
GGGCAATCCTGAGCCAACTCCTTTTTTCAATAGCAAGAATAAAAAAAAAG3540.18585018585018584No Hit
GGGCAATCCTGAGCCAAATCCTGTTTTCTCAAAACAAAAGTTCAAAAAAC3460.18165018165018165No Hit
GGTCAATCCTGAGCCAAATCACGTTTTCCGAAAACAAACAACGGTTCAGA3330.17482517482517482No Hit
GGTCAATCCTGAGCCAACTCCTTTTTTCAAAAGCAAGAATAAAAAAAAAG3190.16747516747516747No Hit
CCATTGAGTCTCTGCACCTATCCCTTTTTATTCTATTTTTTAAAACCTTG3170.16642516642516642No Hit
GGGCAATCCTGAGCCAAATCAAGTTTTCCGAAAACAAACAACGGTTCAGA3110.16327516327516328No Hit
GGGCAATCCTGAGCCAAATCTTTATTTTTTGAAAAACAAGGTTTTAAAAA3070.16117516117516117No Hit
GCGCAATCCTGAGCCAACTCCTTTTTTCAAAAGCAAGAATAAAAAAAAAG2890.15172515172515172No Hit
GCGCAATCCTGAGCCAAATCACGTTTTCCGAAAACAAACAACGGTTCAGA2880.1512001512001512No Hit
CCATTGAGTCTCTGCACCTATCCTTTTTTTTTATTCTAGCTATTGAAAAA2870.1506751506751507No Hit
CCATTGAGTCTCTGCACCTATCCCTTTTTCACCTTCTGGGCCTTTGTTTG2850.1496251496251496No Hit
CCATTGAGTCTCTGCACCTATCCATTTTTTTTATTCTTGCTTTTGAAAAA2780.14595014595014594No Hit
CCATTGAGTCTCTGCACCTATCCTTTTTGATTTTCGCTTTCAGAACCGTT2560.1344001344001344No Hit
CCATTGAGTCTCTGCACCTATCCTTTTTTATTCTCTTTTTTAAACCCTTG2460.12915012915012913No Hit
GGGCAATCCTGAGCCAACTCCTTTTTTCATAAGCAAGAATAAAAAAAAAG2420.12705012705012705No Hit
GGGCAATCCTGAGCCAACTCCTTTTTTCAAAAGCAAGAATAAAAAAAAAT2390.12547512547512546No Hit
GGGCAATCCTGAGCCAAATCTTTATTTTTTGAAAAACAAGGGTTTAAAAA2250.11812511812511813No Hit
CCATTGAGTCTCTGCACCTACCCCTTTTTTTTTTTCGGAAAAAGGATTTG2240.1176001176001176No Hit
CCATTGAGTCTCTGCACCTATCCTTTTTTTTTTATTCTTGTTTTCGGAAT2180.11445011445011447No Hit
CCATTGAGTCTCTGCACCTATCCATTTTGATTTTCGCTTTCTGAACCGTT2150.11287511287511288No Hit
CCATTGAGTCTCTGCACCTATCCTTTTTTTTTATTCTAGCTTTTGAAAAA2120.1113001113001113No Hit
GGGAAATCCTGAGCCAAATCACGTTTTCCGAAAACAAACAACGGTTCAGA1990.10447510447510447No Hit
CCATTGAGTCTCTGCACCTATCCTTTTTTTTTATTATTGCTTTTGAAAAA1950.10237510237510238No Hit
GGCCAATCCTGAGCCAAATCACGTTTTCCGAAAACAAACAACGGTTCAGA1910.10027510027510028No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)